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The map label for this gene is nusA [H]

Identifier: 83595108

GI number: 83595108

Start: 4327448

End: 4328998

Strand: Direct

Name: nusA [H]

Synonym: Rru_A3779

Alternate gene names: 83595108

Gene position: 4327448-4328998 (Clockwise)

Preceding gene: 83595107

Following gene: 83595109

Centisome position: 99.42

GC content: 64.28

Gene sequence:

>1551_bases
ATGGAACGAACCGTTGGCATGAGCCGGCCCGAACTAGTCCAGGTGGCCGATACCGTGGCCCGCGACAAGAGCATCGAGCG
CGAGGAAGTGTTCGCCGCCATGGAGCAGGCCATTCAGAAGGCCGGTCGCTCCAAATACGGCCATGAACACGATATCCGGG
CGCGCATCGATCGCAAGACCGGCGAAATCCGTCTGGCCCGTTACATCGAAGTGGTCGAGGAGGTGGAGAACGAATTCACC
CAGATCACCCTGGCCGGCGCCAAGCGCAAGAAAGCCGATATCGAACTGGGTGAATTCCTGGTCGATCCCCTGCCCCCCAT
CGACTTCGGGCGGATCGCCGCCCAGACCGCCAAGCAGGTGATCGTTCAGAAGGTCCGCGACGCCGAGCGCGAGCGCCAGT
TCGCCGAATACAAGGATCGCCTGGGCGAGATCATCAATGGTCTGGTCAAGCGCGTCGAATTCGGCAATGTCATCGTCGAC
ATGGGCCGGGCCGAAGCGCTGCTGCGCCGGGACGAGGTCATCCCGCGCGAGCACTTCAAGAACGGCGACCGCATCCGCGC
CTATATCATGGACGTTCGCCAGGAACCCCGCGGCCCGCAGATCTTCCTGTCGCGGACCCACGAGCAGTTCATGGCCAAGC
TGTTCGCCCAGGAAGTGCCCGAAATCTACGACGGCATCATCGAGATCAAATCGGTGGCGCGCGATCCCGGATCGCGCGCC
AAGATCGCCGTGCTGTCCAACGACCACGCCATCGATCCGGTCGGCGCCTGCGTCGGCATGCGCGGCAGCCGCGTTCAGGC
GGTGGTCGCCGAGCTTCAGGGCGAGAAGATCGACATCATTCAATGGGCGACCGATCCGGCGACCTTCGTCGTCAACGCCC
TGGCCCCGGCCGAGGTCGCCAAGGTCGTGCTTGACGAGGAAGCCAACCGCATCGAAGTGGTGGTGCCCGACGAGCAATTG
TCGCTGGCCATCGGCCGGCGCGGGCAGAACGTCCGTCTGGCCTCGAAGCTGACCGGCTGGGATATCGATATCCTGACCGA
GGCCGAGGAAAGCGAACGCCGCCAGGACGAATTCCGCATCCGCACCCAGGCCTTCATCGAAGCCCTGGACGTTGACGACG
TCATCGCCCACCTTCTGGTGACCGAAGGCTTCACCACCGTCGAGGACGTGGCCTTCGTGGCCATTGCCGAGTTGAGCGAG
ATCGAAGGCTTCGACGAGGACGTGGCCTCCGAGCTGCAGAACCGCGCCCAGGTCTTCCTGGCCGAACGCGAGCGTTTGAA
CGAAGAGCGCCGCAAGGAACTGGGCGTCGTCGACGAGATGGCCGAAATCGAGGGGCTGACCGCCGCCATGCTGGTCGCCC
TGGGCGAAAAGGGCGTGAAAACCCTCGACGATCTCGGCGATCTGGCCAGCGACGAATTGATCGAGATCGTTGGCGGCCTG
AATGAGGACGACGCCAACGCCGTGATCATGGCGGCGCGCGCCCATTGGTTCGGCGAAGCCGGCGACTCGGCCGACGACGC
GACGGCGAAGACCCCGGAGCAGGATGCCTGA

Upstream 100 bases:

>100_bases
AGGGAGAGGCCGCCGGTGGCGCGGTCGAGGGCGGCGTAGCCTGACCAACGGCCCGCCCGCCGACCGAACAATCAGGTCGA
ACAACCAGGATAGCGAAACG

Downstream 100 bases:

>100_bases
TCCGATGGACGATCCCGCCCTGGACGACGACTCCGCCGACGACACGGCGCCGCCCGCCTCCGGGAAGCGTGTGCCCGCCG
ACGATCGCGAGCCCGAAGGG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MERTVGMSRPELVQVADTVARDKSIEREEVFAAMEQAIQKAGRSKYGHEHDIRARIDRKTGEIRLARYIEVVEEVENEFT
QITLAGAKRKKADIELGEFLVDPLPPIDFGRIAAQTAKQVIVQKVRDAERERQFAEYKDRLGEIINGLVKRVEFGNVIVD
MGRAEALLRRDEVIPREHFKNGDRIRAYIMDVRQEPRGPQIFLSRTHEQFMAKLFAQEVPEIYDGIIEIKSVARDPGSRA
KIAVLSNDHAIDPVGACVGMRGSRVQAVVAELQGEKIDIIQWATDPATFVVNALAPAEVAKVVLDEEANRIEVVVPDEQL
SLAIGRRGQNVRLASKLTGWDIDILTEAEESERRQDEFRIRTQAFIEALDVDDVIAHLLVTEGFTTVEDVAFVAIAELSE
IEGFDEDVASELQNRAQVFLAERERLNEERRKELGVVDEMAEIEGLTAAMLVALGEKGVKTLDDLGDLASDELIEIVGGL
NEDDANAVIMAARAHWFGEAGDSADDATAKTPEQDA

Sequences:

>Translated_516_residues
MERTVGMSRPELVQVADTVARDKSIEREEVFAAMEQAIQKAGRSKYGHEHDIRARIDRKTGEIRLARYIEVVEEVENEFT
QITLAGAKRKKADIELGEFLVDPLPPIDFGRIAAQTAKQVIVQKVRDAERERQFAEYKDRLGEIINGLVKRVEFGNVIVD
MGRAEALLRRDEVIPREHFKNGDRIRAYIMDVRQEPRGPQIFLSRTHEQFMAKLFAQEVPEIYDGIIEIKSVARDPGSRA
KIAVLSNDHAIDPVGACVGMRGSRVQAVVAELQGEKIDIIQWATDPATFVVNALAPAEVAKVVLDEEANRIEVVVPDEQL
SLAIGRRGQNVRLASKLTGWDIDILTEAEESERRQDEFRIRTQAFIEALDVDDVIAHLLVTEGFTTVEDVAFVAIAELSE
IEGFDEDVASELQNRAQVFLAERERLNEERRKELGVVDEMAEIEGLTAAMLVALGEKGVKTLDDLGDLASDELIEIVGGL
NEDDANAVIMAARAHWFGEAGDSADDATAKTPEQDA
>Mature_516_residues
MERTVGMSRPELVQVADTVARDKSIEREEVFAAMEQAIQKAGRSKYGHEHDIRARIDRKTGEIRLARYIEVVEEVENEFT
QITLAGAKRKKADIELGEFLVDPLPPIDFGRIAAQTAKQVIVQKVRDAERERQFAEYKDRLGEIINGLVKRVEFGNVIVD
MGRAEALLRRDEVIPREHFKNGDRIRAYIMDVRQEPRGPQIFLSRTHEQFMAKLFAQEVPEIYDGIIEIKSVARDPGSRA
KIAVLSNDHAIDPVGACVGMRGSRVQAVVAELQGEKIDIIQWATDPATFVVNALAPAEVAKVVLDEEANRIEVVVPDEQL
SLAIGRRGQNVRLASKLTGWDIDILTEAEESERRQDEFRIRTQAFIEALDVDDVIAHLLVTEGFTTVEDVAFVAIAELSE
IEGFDEDVASELQNRAQVFLAERERLNEERRKELGVVDEMAEIEGLTAAMLVALGEKGVKTLDDLGDLASDELIEIVGGL
NEDDANAVIMAARAHWFGEAGDSADDATAKTPEQDA

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=491, Percent_Identity=44.806517311609, Blast_Score=410, Evalue=1e-116,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 57469; Mature: 57469

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERTVGMSRPELVQVADTVARDKSIEREEVFAAMEQAIQKAGRSKYGHEHDIRARIDRKT
CCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCC
GEIRLARYIEVVEEVENEFTQITLAGAKRKKADIELGEFLVDPLPPIDFGRIAAQTAKQV
CHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHH
IVQKVRDAERERQFAEYKDRLGEIINGLVKRVEFGNVIVDMGRAEALLRRDEVIPREHFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECHHHHHHHHHHCCCCHHHCC
NGDRIRAYIMDVRQEPRGPQIFLSRTHEQFMAKLFAQEVPEIYDGIIEIKSVARDPGSRA
CCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KIAVLSNDHAIDPVGACVGMRGSRVQAVVAELQGEKIDIIQWATDPATFVVNALAPAEVA
EEEEECCCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCHHHHH
KVVLDEEANRIEVVVPDEQLSLAIGRRGQNVRLASKLTGWDIDILTEAEESERRQDEFRI
HHHHCCCCCEEEEEECCHHHHHHHCCCCCCEEEHHHCCCCCEEEEECCHHHHHHHHHHHH
RTQAFIEALDVDDVIAHLLVTEGFTTVEDVAFVAIAELSEIEGFDEDVASELQNRAQVFL
HHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AERERLNEERRKELGVVDEMAEIEGLTAAMLVALGEKGVKTLDDLGDLASDELIEIVGGL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
NEDDANAVIMAARAHWFGEAGDSADDATAKTPEQDA
CCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MERTVGMSRPELVQVADTVARDKSIEREEVFAAMEQAIQKAGRSKYGHEHDIRARIDRKT
CCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCC
GEIRLARYIEVVEEVENEFTQITLAGAKRKKADIELGEFLVDPLPPIDFGRIAAQTAKQV
CHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHH
IVQKVRDAERERQFAEYKDRLGEIINGLVKRVEFGNVIVDMGRAEALLRRDEVIPREHFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECHHHHHHHHHHCCCCHHHCC
NGDRIRAYIMDVRQEPRGPQIFLSRTHEQFMAKLFAQEVPEIYDGIIEIKSVARDPGSRA
CCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KIAVLSNDHAIDPVGACVGMRGSRVQAVVAELQGEKIDIIQWATDPATFVVNALAPAEVA
EEEEECCCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCHHHHH
KVVLDEEANRIEVVVPDEQLSLAIGRRGQNVRLASKLTGWDIDILTEAEESERRQDEFRI
HHHHCCCCCEEEEEECCHHHHHHHCCCCCCEEEHHHCCCCCEEEEECCHHHHHHHHHHHH
RTQAFIEALDVDDVIAHLLVTEGFTTVEDVAFVAIAELSEIEGFDEDVASELQNRAQVFL
HHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AERERLNEERRKELGVVDEMAEIEGLTAAMLVALGEKGVKTLDDLGDLASDELIEIVGGL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
NEDDANAVIMAARAHWFGEAGDSADDATAKTPEQDA
CCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]