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The map label for this gene is braC [H]

Identifier: 83595057

GI number: 83595057

Start: 4279834

End: 4281012

Strand: Direct

Name: braC [H]

Synonym: Rru_A3728

Alternate gene names: 83595057

Gene position: 4279834-4281012 (Clockwise)

Preceding gene: 83595056

Following gene: 83595058

Centisome position: 98.32

GC content: 64.72

Gene sequence:

>1179_bases
ATGCCTTTCGTTTCCACGACACGCGCCGGCCTGTTGGCGGGCGCCTTCGCCCTGGCCATCGGCTTCGCCGGGCCGGCGGC
CAGCGCCGATCCGATCAAGATCGGCGGCGCCTTCAATCTGACGGGTGGTCAGGCCTCGCTTGACGGTCCGGCGAAGAATG
GCGCCCAACTCGCCATTGATACCCTGAACGCCGCCGGCGGCGTCAATGGCACCCCGCTCGAGCTGGTGGTCTATGACGGC
AAGACCGATCCGGCCGTGGTCGCCTCGCTGGGCAGCCAGTTGATCAATTCCGATAAGGTCAGCGCGATCATCGGCTTTTC
GGATTCCGATCCGGTTCTGGCCCTTGGTCCCAATGCCCAGAAGGCCGGCGTGCCGTTCATCGCCGTCGGAGCGACCTCGC
CCAAGCTGCCCGACCAGATCGGCGACACCATGTTCCTGGCCTGCTTTGGCGATAACACCCAGGCCGCCGTCGGCGCCTCT
TTCGCCATCGACGATCTGAAGGCCAAAAGCGTCTATGTCCTTGAAGATACCGCCAATGAATACGCGACCTTGCTGTCGAA
GTATTTCCGCGAGACCTTCGAGCATCTGGGCGGCAAGGTGATCGGGCGCGACACCTATCGCACCGGCGACAAAAGCTTCA
CCGCCCAGATCACCAAGATCAAGGCGGCGGCCGAGAAGCCCGACATCCTTTACATCGCCGCCACGCCCGATTCGATCGGC
TTGGTGGTGCGTCAGGTCCGTCAGGCCGGCCTGAAGCTGCCGATCGTCGGCGGGGATGGCTATGACACGCCGCTGCTGCT
CGAAGTGGGCGGCGCTTCGGCCAATAACGTCTATTTCACCACCCATTCCTTCGTGTCCGACAGCGCGCCCGGCCCGATGA
AGACGTTCTTCGACGCCTACGGCAAGGCCTATGGCAACGCCCCCGAGAACGCCTTCGCCGCCCTTGGCTATGACACGGTG
ATGCTGGTCGCCGATGCCATCAAGCGGGCCGGTTCGGGTGATCCCGCCGCCATCCGCAAGGCCCTGACCGAGACCAAGGA
TCTGGCCGGGGTGACCGGCGCGGTCACCTTCGCCGAGGGCAGCCGCGTTCCGGTCAAGAGCGTGACCGTCATCGGCGTCA
AGGACAAGGCGCTCTACAAGGCGGCCGAGATGGCGCCGACCTTCGTGCCCAAGCCCTGA

Upstream 100 bases:

>100_bases
ATGATCTACCGGCCGCGCGGCCTTCTCGAAGACCGCGAGCTGACGTCGTTTGGCCGCAAGGGCTGACGGCGGCGTTCTCC
TCCCACTGAAGGAACATGGC

Downstream 100 bases:

>100_bases
TCGGTCGGGCTTCGCCCGTCGTCGCGCCCCGGGCCGGGATGGTCCGACCCGGGGCGGCCTTTCCGTCGTTGCGCTTTACC
GGCTCGCCACTTCCCCCATC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MPFVSTTRAGLLAGAFALAIGFAGPAASADPIKIGGAFNLTGGQASLDGPAKNGAQLAIDTLNAAGGVNGTPLELVVYDG
KTDPAVVASLGSQLINSDKVSAIIGFSDSDPVLALGPNAQKAGVPFIAVGATSPKLPDQIGDTMFLACFGDNTQAAVGAS
FAIDDLKAKSVYVLEDTANEYATLLSKYFRETFEHLGGKVIGRDTYRTGDKSFTAQITKIKAAAEKPDILYIAATPDSIG
LVVRQVRQAGLKLPIVGGDGYDTPLLLEVGGASANNVYFTTHSFVSDSAPGPMKTFFDAYGKAYGNAPENAFAALGYDTV
MLVADAIKRAGSGDPAAIRKALTETKDLAGVTGAVTFAEGSRVPVKSVTVIGVKDKALYKAAEMAPTFVPKP

Sequences:

>Translated_392_residues
MPFVSTTRAGLLAGAFALAIGFAGPAASADPIKIGGAFNLTGGQASLDGPAKNGAQLAIDTLNAAGGVNGTPLELVVYDG
KTDPAVVASLGSQLINSDKVSAIIGFSDSDPVLALGPNAQKAGVPFIAVGATSPKLPDQIGDTMFLACFGDNTQAAVGAS
FAIDDLKAKSVYVLEDTANEYATLLSKYFRETFEHLGGKVIGRDTYRTGDKSFTAQITKIKAAAEKPDILYIAATPDSIG
LVVRQVRQAGLKLPIVGGDGYDTPLLLEVGGASANNVYFTTHSFVSDSAPGPMKTFFDAYGKAYGNAPENAFAALGYDTV
MLVADAIKRAGSGDPAAIRKALTETKDLAGVTGAVTFAEGSRVPVKSVTVIGVKDKALYKAAEMAPTFVPKP
>Mature_391_residues
PFVSTTRAGLLAGAFALAIGFAGPAASADPIKIGGAFNLTGGQASLDGPAKNGAQLAIDTLNAAGGVNGTPLELVVYDGK
TDPAVVASLGSQLINSDKVSAIIGFSDSDPVLALGPNAQKAGVPFIAVGATSPKLPDQIGDTMFLACFGDNTQAAVGASF
AIDDLKAKSVYVLEDTANEYATLLSKYFRETFEHLGGKVIGRDTYRTGDKSFTAQITKIKAAAEKPDILYIAATPDSIGL
VVRQVRQAGLKLPIVGGDGYDTPLLLEVGGASANNVYFTTHSFVSDSAPGPMKTFFDAYGKAYGNAPENAFAALGYDTVM
LVADAIKRAGSGDPAAIRKALTETKDLAGVTGAVTFAEGSRVPVKSVTVIGVKDKALYKAAEMAPTFVPKP

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=334, Percent_Identity=27.8443113772455, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI48994941, Length=350, Percent_Identity=27.1428571428571, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40193; Mature: 40062

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFVSTTRAGLLAGAFALAIGFAGPAASADPIKIGGAFNLTGGQASLDGPAKNGAQLAID
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEECCCCCCCCCCCCCCCEEEEE
TLNAAGGVNGTPLELVVYDGKTDPAVVASLGSQLINSDKVSAIIGFSDSDPVLALGPNAQ
EECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCC
KAGVPFIAVGATSPKLPDQIGDTMFLACFGDNTQAAVGASFAIDDLKAKSVYVLEDTANE
CCCCCEEEECCCCCCCCHHCCCEEEEEEECCCCCHHHCCEEEECCCCCCEEEEEECCHHH
YATLLSKYFRETFEHLGGKVIGRDTYRTGDKSFTAQITKIKAAAEKPDILYIAATPDSIG
HHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEEEEEHHCCCCCEEEEEECCCHHH
LVVRQVRQAGLKLPIVGGDGYDTPLLLEVGGASANNVYFTTHSFVSDSAPGPMKTFFDAY
HHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHH
GKAYGNAPENAFAALGYDTVMLVADAIKRAGSGDPAAIRKALTETKDLAGVTGAVTFAEG
HHHCCCCCCCCEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCC
SRVPVKSVTVIGVKDKALYKAAEMAPTFVPKP
CCCCCCEEEEEEECCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PFVSTTRAGLLAGAFALAIGFAGPAASADPIKIGGAFNLTGGQASLDGPAKNGAQLAID
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEECCCCCCCCCCCCCCCEEEEE
TLNAAGGVNGTPLELVVYDGKTDPAVVASLGSQLINSDKVSAIIGFSDSDPVLALGPNAQ
EECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCC
KAGVPFIAVGATSPKLPDQIGDTMFLACFGDNTQAAVGASFAIDDLKAKSVYVLEDTANE
CCCCCEEEECCCCCCCCHHCCCEEEEEEECCCCCHHHCCEEEECCCCCCEEEEEECCHHH
YATLLSKYFRETFEHLGGKVIGRDTYRTGDKSFTAQITKIKAAAEKPDILYIAATPDSIG
HHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEEEEEHHCCCCCEEEEEECCCHHH
LVVRQVRQAGLKLPIVGGDGYDTPLLLEVGGASANNVYFTTHSFVSDSAPGPMKTFFDAY
HHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHH
GKAYGNAPENAFAALGYDTVMLVADAIKRAGSGDPAAIRKALTETKDLAGVTGAVTFAEG
HHHCCCCCCCCEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCC
SRVPVKSVTVIGVKDKALYKAAEMAPTFVPKP
CCCCCCEEEEEEECCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]