Click here to switch to the map view.

The map label for this gene is ubiF [H]

Identifier: 83595036

GI number: 83595036

Start: 4257437

End: 4258726

Strand: Direct

Name: ubiF [H]

Synonym: Rru_A3707

Alternate gene names: 83595036

Gene position: 4257437-4258726 (Clockwise)

Preceding gene: 83595033

Following gene: 83595041

Centisome position: 97.81

GC content: 69.84

Gene sequence:

>1290_bases
GTGAGCGAACCCCTGTTGCGCGGCCTTGCCGCCGGCGATCCCCCTTCGGCCACTGGGCCGGTCACTGGGTCCGCCGATAA
GGTCGCCGATGTTTTGATCGTCGGCGGTGGACTGGTCGGCGGAACCCTGGCCTGCGCCCTCGCCGAAAAAGGCGTCTCGG
TGGTGGTGATCGATGGCGAGGATCCCGAGGCCCTGCTCGCCGCCGGCTATGACGGCCGCTGCTCGGCCATCGCCCTGGCC
TGCCAGCGGTTGCTTGACACCATCGGCCTGTGGGATCTGCTGGGCGGTGAAAGCCAACCGATCCTCGACATCCGCGTCGT
CGATGGCGGCTCGCCGCTGTTTCTCCATTACGCCCAGGCCGAGGCCCAGGGCCCGATGGGCTATATGGTCGAGAACCGGT
TGCTGCGCCAAGCCATCCTGACCCGGCTTGGCCGGCTGCCCGCCGCCACCTTGCTCGCTCCGGCGCGCATGACCGCCCTG
CGCCGCGACCTCGACGGCGTCAGCGCCACCTTGTCGGATGGCCAAACGGTGCGCGCCCGGCTGGTGGTCGGCGCCGATGG
CCGGCGGTCCCAGGTGCGCGAGAGCGCCGGGATCGGCATCCGCACCCTGGGCTATGGCCAAACCGCCATCGTGCTGACGG
TCGAGCACGAGCGTTCCCATCGCGGCTGCGCGGTGGAGCATTTCCTGCCCGCCGGTCCCTTCGCCATCCTGCCGATGCCC
GGCAACCGCTCCTCGCTGGTCTGGACCGAGCGGAGCGATCTGGTGCCCGGCCTGCTCGCCCTGCCGGCTGAACACTTCCA
GGCCGAATTAGAGCGGCGCTTTGGCGATCATCTGGGCTGGGTGCGTCCGGTCGGGCCGCGCTTTTCCTATCGGCTGACCC
TGCAGGCGGCCAACCGCTATGTCGACCACCGGCTGGCCCTGGTTGGCGATGCTGCCCACGGCATGCATCCGGTGGCCGGT
CAGGGCATGAACTATGGCCTGCGCGACGTGGCGGTGCTGGCCGAACGGCTGGTCGCCGCCCAGCGCCTTGGCCTTGACCC
CGGCGCCCCCGCTCTGCTGGCCGAGTACGAGGCGCTGCGCCGCCCCGATAATTTGCTGATGCTGGCGATCACCGACGCCC
TGGTTCGGCTGTTTTCCAATGATATCGCCCCCGTCGCCCTCGCCCGCCGCCTGGGCATCGGCGCCGTCGAGCGCATGGGG
CCGCTCAAGCGGTTGTTCATGCGCCACGCCATGGGCACCTTGAAGCTGGGGCCCGAGCCGCCGCGCCTGATGCGTGGGGT
TCCCTTGTAA

Upstream 100 bases:

>100_bases
CGGCGGCGAACGCCATGTCTGAAGTATCGTTTCCCGGCGCTTCGCGCCTGTCCGGCCCCGCTTTCCCCCGGCCGATCCCC
CGATTTCGATGGAGTACGGT

Downstream 100 bases:

>100_bases
GGTCGGGCCGGCCGAGATCCGTGGTCGCCGGTTCTTCCAGAAAAGCCGTTAGCGCCGTTTTTTAGATTACGCGCCGCACT
CCGGGAACGAGGCGTAAAGC

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase / 2-octaprenyl-6-methoxyphenol hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSEPLLRGLAAGDPPSATGPVTGSADKVADVLIVGGGLVGGTLACALAEKGVSVVVIDGEDPEALLAAGYDGRCSAIALA
CQRLLDTIGLWDLLGGESQPILDIRVVDGGSPLFLHYAQAEAQGPMGYMVENRLLRQAILTRLGRLPAATLLAPARMTAL
RRDLDGVSATLSDGQTVRARLVVGADGRRSQVRESAGIGIRTLGYGQTAIVLTVEHERSHRGCAVEHFLPAGPFAILPMP
GNRSSLVWTERSDLVPGLLALPAEHFQAELERRFGDHLGWVRPVGPRFSYRLTLQAANRYVDHRLALVGDAAHGMHPVAG
QGMNYGLRDVAVLAERLVAAQRLGLDPGAPALLAEYEALRRPDNLLMLAITDALVRLFSNDIAPVALARRLGIGAVERMG
PLKRLFMRHAMGTLKLGPEPPRLMRGVPL

Sequences:

>Translated_429_residues
MSEPLLRGLAAGDPPSATGPVTGSADKVADVLIVGGGLVGGTLACALAEKGVSVVVIDGEDPEALLAAGYDGRCSAIALA
CQRLLDTIGLWDLLGGESQPILDIRVVDGGSPLFLHYAQAEAQGPMGYMVENRLLRQAILTRLGRLPAATLLAPARMTAL
RRDLDGVSATLSDGQTVRARLVVGADGRRSQVRESAGIGIRTLGYGQTAIVLTVEHERSHRGCAVEHFLPAGPFAILPMP
GNRSSLVWTERSDLVPGLLALPAEHFQAELERRFGDHLGWVRPVGPRFSYRLTLQAANRYVDHRLALVGDAAHGMHPVAG
QGMNYGLRDVAVLAERLVAAQRLGLDPGAPALLAEYEALRRPDNLLMLAITDALVRLFSNDIAPVALARRLGIGAVERMG
PLKRLFMRHAMGTLKLGPEPPRLMRGVPL
>Mature_428_residues
SEPLLRGLAAGDPPSATGPVTGSADKVADVLIVGGGLVGGTLACALAEKGVSVVVIDGEDPEALLAAGYDGRCSAIALAC
QRLLDTIGLWDLLGGESQPILDIRVVDGGSPLFLHYAQAEAQGPMGYMVENRLLRQAILTRLGRLPAATLLAPARMTALR
RDLDGVSATLSDGQTVRARLVVGADGRRSQVRESAGIGIRTLGYGQTAIVLTVEHERSHRGCAVEHFLPAGPFAILPMPG
NRSSLVWTERSDLVPGLLALPAEHFQAELERRFGDHLGWVRPVGPRFSYRLTLQAANRYVDHRLALVGDAAHGMHPVAGQ
GMNYGLRDVAVLAERLVAAQRLGLDPGAPALLAEYEALRRPDNLLMLAITDALVRLFSNDIAPVALARRLGIGAVERMGP
LKRLFMRHAMGTLKLGPEPPRLMRGVPL

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=440, Percent_Identity=31.1363636363636, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI32967307, Length=330, Percent_Identity=32.7272727272727, Blast_Score=155, Evalue=6e-38,
Organism=Escherichia coli, GI1786883, Length=390, Percent_Identity=37.6923076923077, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI1789274, Length=387, Percent_Identity=35.6589147286822, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1789273, Length=399, Percent_Identity=34.3358395989975, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI25146631, Length=435, Percent_Identity=26.8965517241379, Blast_Score=159, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6321694, Length=121, Percent_Identity=39.6694214876033, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19920760, Length=458, Percent_Identity=29.9126637554585, Blast_Score=156, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 45619; Mature: 45487

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPLLRGLAAGDPPSATGPVTGSADKVADVLIVGGGLVGGTLACALAEKGVSVVVIDGE
CCCHHHHHHCCCCCCCCCCCCCCCHHHHEEEEEECCCHHHHHHHHHHHHCCCEEEEECCC
DPEALLAAGYDGRCSAIALACQRLLDTIGLWDLLGGESQPILDIRVVDGGSPLFLHYAQA
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECC
EAQGPMGYMVENRLLRQAILTRLGRLPAATLLAPARMTALRRDLDGVSATLSDGQTVRAR
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEE
LVVGADGRRSQVRESAGIGIRTLGYGQTAIVLTVEHERSHRGCAVEHFLPAGPFAILPMP
EEECCCCCHHHHHHHCCCCEEEECCCCEEEEEEEECCCCCCCCCHHHCCCCCCEEEEECC
GNRSSLVWTERSDLVPGLLALPAEHFQAELERRFGDHLGWVRPVGPRFSYRLTLQAANRY
CCCCCEEEECCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHH
VDHRLALVGDAAHGMHPVAGQGMNYGLRDVAVLAERLVAAQRLGLDPGAPALLAEYEALR
HHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
RPDNLLMLAITDALVRLFSNDIAPVALARRLGIGAVERMGPLKRLFMRHAMGTLKLGPEP
CCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
PRLMRGVPL
HHHHCCCCC
>Mature Secondary Structure 
SEPLLRGLAAGDPPSATGPVTGSADKVADVLIVGGGLVGGTLACALAEKGVSVVVIDGE
CCHHHHHHCCCCCCCCCCCCCCCHHHHEEEEEECCCHHHHHHHHHHHHCCCEEEEECCC
DPEALLAAGYDGRCSAIALACQRLLDTIGLWDLLGGESQPILDIRVVDGGSPLFLHYAQA
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECC
EAQGPMGYMVENRLLRQAILTRLGRLPAATLLAPARMTALRRDLDGVSATLSDGQTVRAR
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEE
LVVGADGRRSQVRESAGIGIRTLGYGQTAIVLTVEHERSHRGCAVEHFLPAGPFAILPMP
EEECCCCCHHHHHHHCCCCEEEECCCCEEEEEEEECCCCCCCCCHHHCCCCCCEEEEECC
GNRSSLVWTERSDLVPGLLALPAEHFQAELERRFGDHLGWVRPVGPRFSYRLTLQAANRY
CCCCCEEEECCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHH
VDHRLALVGDAAHGMHPVAGQGMNYGLRDVAVLAERLVAAQRLGLDPGAPALLAEYEALR
HHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
RPDNLLMLAITDALVRLFSNDIAPVALARRLGIGAVERMGPLKRLFMRHAMGTLKLGPEP
CCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
PRLMRGVPL
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]