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The map label for this gene is xthA [H]

Identifier: 83595025

GI number: 83595025

Start: 4242744

End: 4243493

Strand: Reverse

Name: xthA [H]

Synonym: Rru_A3696

Alternate gene names: 83595025

Gene position: 4243493-4242744 (Counterclockwise)

Preceding gene: 83595026

Following gene: 83595022

Centisome position: 97.49

GC content: 66.8

Gene sequence:

>750_bases
GTGGCGCGGCTGGTTGAAAGCCGGGCGATCGACGTGTTGTGTCTCCAGGAGATCAAGGCGCGGCCCGACCATGTGCCGAC
CGCCGCCCTGGCCGAGATGGGGCTGGTCCATCAGGCCCTTCACACCATGCCCGGCTATAACGGCGTCGGCATCTTCTCGC
GCCTACCCCTGGAAGACAGCACGCCCTTTGACTGGTGCGACCGCGTCGATTGCCGCCACCTGCGCACCCGGGTCGCCGGC
TTCACCCTCCACTGCCTTTATATCCCGGCCGGCGGCGATATCCCCGATCCCGAGGAAAATCCCCGCTTCCGCCACAAGCT
GGCGTTCTTCGACGCCCTTACCGACTGGTTCGACCGCACCCTTGATCCCGACATGCCGGCGATGGTGGTGGGCGATTTCA
ATGTCGCCCCGCTCGAGACCGATGTCTGGGATCACCGCAAGATGCGCGGCGTGGTCAGCCATACGCCGGTCGAGGTCGCC
AAGCTGGGCGCCCTGCAGGCCAGCGGGCCGTGGATCGACGCCATGCGCCGCTTCGTGCCGCCCGAGGAAAAGCTGTTCAC
CTGGTGGAGCTACCGGGCCAAGGACTGGCGGCTGACCAATCGCGGCCGGCGGCTTGATCACATCTGGGTCACCCCCGCCC
TGGTCGATCGCCTGCGCGGCCTGGAGGTGATGGACGACACCCGCGACTGGACCCCGCCCTCGGACCATGTGCCGGTGATC
GTGCATATCGACCCGCCAACAAAGGGCTAA

Upstream 100 bases:

>100_bases
CCCTCGTCGCCGGACCGGTCCTCTGAGCCCCCCGGCTTCGCTCCGACCGCTGGCGATCGCCACCTGGAACATCAACTCGC
TGCGCCTGCGCCTGGATCAG

Downstream 100 bases:

>100_bases
GCCCTATCCCGCCAGTTCGGGGCGTCCGGCGAAGTCGTCGAGCGCCTCGGGGTTGGCCAGGGCCTCGCGGTTGCGCACCG
GCCGGCCGTGGACGACCTCG

Product: exodeoxyribonuclease III xth

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MARLVESRAIDVLCLQEIKARPDHVPTAALAEMGLVHQALHTMPGYNGVGIFSRLPLEDSTPFDWCDRVDCRHLRTRVAG
FTLHCLYIPAGGDIPDPEENPRFRHKLAFFDALTDWFDRTLDPDMPAMVVGDFNVAPLETDVWDHRKMRGVVSHTPVEVA
KLGALQASGPWIDAMRRFVPPEEKLFTWWSYRAKDWRLTNRGRRLDHIWVTPALVDRLRGLEVMDDTRDWTPPSDHVPVI
VHIDPPTKG

Sequences:

>Translated_249_residues
MARLVESRAIDVLCLQEIKARPDHVPTAALAEMGLVHQALHTMPGYNGVGIFSRLPLEDSTPFDWCDRVDCRHLRTRVAG
FTLHCLYIPAGGDIPDPEENPRFRHKLAFFDALTDWFDRTLDPDMPAMVVGDFNVAPLETDVWDHRKMRGVVSHTPVEVA
KLGALQASGPWIDAMRRFVPPEEKLFTWWSYRAKDWRLTNRGRRLDHIWVTPALVDRLRGLEVMDDTRDWTPPSDHVPVI
VHIDPPTKG
>Mature_248_residues
ARLVESRAIDVLCLQEIKARPDHVPTAALAEMGLVHQALHTMPGYNGVGIFSRLPLEDSTPFDWCDRVDCRHLRTRVAGF
TLHCLYIPAGGDIPDPEENPRFRHKLAFFDALTDWFDRTLDPDMPAMVVGDFNVAPLETDVWDHRKMRGVVSHTPVEVAK
LGALQASGPWIDAMRRFVPPEEKLFTWWSYRAKDWRLTNRGRRLDHIWVTPALVDRLRGLEVMDDTRDWTPPSDHVPVIV
HIDPPTKG

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=247, Percent_Identity=29.9595141700405, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI18375503, Length=247, Percent_Identity=29.9595141700405, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI18375501, Length=247, Percent_Identity=29.9595141700405, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1788046, Length=248, Percent_Identity=26.2096774193548, Blast_Score=77, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221330655, Length=247, Percent_Identity=27.1255060728745, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17136678, Length=247, Percent_Identity=27.1255060728745, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 28468; Mature: 28337

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLVESRAIDVLCLQEIKARPDHVPTAALAEMGLVHQALHTMPGYNGVGIFSRLPLEDS
CCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCC
TPFDWCDRVDCRHLRTRVAGFTLHCLYIPAGGDIPDPEENPRFRHKLAFFDALTDWFDRT
CCCCHHHCCHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
LDPDMPAMVVGDFNVAPLETDVWDHRKMRGVVSHTPVEVAKLGALQASGPWIDAMRRFVP
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHCC
PEEKLFTWWSYRAKDWRLTNRGRRLDHIWVTPALVDRLRGLEVMDDTRDWTPPSDHVPVI
CHHHEEEEECCCCCCEEECCCCCEEEEEEECHHHHHHHCCCEEECCCCCCCCCCCCCEEE
VHIDPPTKG
EEECCCCCC
>Mature Secondary Structure 
ARLVESRAIDVLCLQEIKARPDHVPTAALAEMGLVHQALHTMPGYNGVGIFSRLPLEDS
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCC
TPFDWCDRVDCRHLRTRVAGFTLHCLYIPAGGDIPDPEENPRFRHKLAFFDALTDWFDRT
CCCCHHHCCHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
LDPDMPAMVVGDFNVAPLETDVWDHRKMRGVVSHTPVEVAKLGALQASGPWIDAMRRFVP
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHCC
PEEKLFTWWSYRAKDWRLTNRGRRLDHIWVTPALVDRLRGLEVMDDTRDWTPPSDHVPVI
CHHHEEEEECCCCCCEEECCCCCEEEEEEECHHHHHHHCCCEEECCCCCCCCCCCCCEEE
VHIDPPTKG
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]