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The map label for this gene is grpE [H]

Identifier: 83594972

GI number: 83594972

Start: 4184109

End: 4184774

Strand: Direct

Name: grpE [H]

Synonym: Rru_A3643

Alternate gene names: 83594972

Gene position: 4184109-4184774 (Clockwise)

Preceding gene: 83594971

Following gene: 83594975

Centisome position: 96.12

GC content: 67.27

Gene sequence:

>666_bases
ATGCCGCAGCACAACGACACGCCCGAAAGCCGGACCCCTCAGGACTCGTCCGCTCCCGCCTCTCCCGCCGCCGCCGCCGA
TGACACCACCGCGGCGGTCGCCGAGGCGGCCGAGGCGATCGCCCGGGCCGACCTCGAGGAGCGGATCACCGCCCTGGAAG
ACGATAATCGTCGCTTGAAGGAAGAGTATCTGCGCGCCCTGGCCGAGGCTCAGAACGCCAAGCGGATGGCCGACAAGCGC
ATCGAGGATAACAGCCGCTACGCGGTATCGAACTTCGCCAAGGCCGTTCTTGGCGTCGCCGATAACCTCGGCCGCGCCCT
GCTGTCCGTTCCGGAGGAGGCGCGCGGCGGCAACGAGATGGTCAAGAACCTCGCCTTCGGCGTGGAACTGACGGCCAAGG
AACTGGAAAACGCCCTGGCCCAATATCAAATCCGCCGGGTCGAGGCGCTGAACGAGGCCTTCGATCCGCATTTCCATCAG
GCGGTGCAGGAAGTCGAAAACACGGCGGTGCCCAATGCCACCATCGTCTCGGTGCTGCAGGACGGCTATGTCATCCATGA
CCGCCTGCTGCGTCCGGCGATGGTCGTGGTGTCGCGGGGCGGCCCCAAGCGCGAGCCCAAGCCCGCCGCCGACAGCGCCG
ACGGCGGCGTCAACACCACCGTCTAG

Upstream 100 bases:

>100_bases
CATTCCCTTGGTGGATTACACCTCCAAGGTGATCAGCCGGATGATCGGCTGAAGCGGCTTTCCTTGAAACCCTGAAGACG
ACTGGAAGACGGAACGCGCC

Downstream 100 bases:

>100_bases
AAAAAATCTCCGAGCGCTCTGGACCAGAGTGCGACGACGATTTTCTTCACCATCCCTCTATTTGCGAGCCTTTATCGGCT
TGATCGACAGCGGCGGGCGC

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MPQHNDTPESRTPQDSSAPASPAAAADDTTAAVAEAAEAIARADLEERITALEDDNRRLKEEYLRALAEAQNAKRMADKR
IEDNSRYAVSNFAKAVLGVADNLGRALLSVPEEARGGNEMVKNLAFGVELTAKELENALAQYQIRRVEALNEAFDPHFHQ
AVQEVENTAVPNATIVSVLQDGYVIHDRLLRPAMVVVSRGGPKREPKPAADSADGGVNTTV

Sequences:

>Translated_221_residues
MPQHNDTPESRTPQDSSAPASPAAAADDTTAAVAEAAEAIARADLEERITALEDDNRRLKEEYLRALAEAQNAKRMADKR
IEDNSRYAVSNFAKAVLGVADNLGRALLSVPEEARGGNEMVKNLAFGVELTAKELENALAQYQIRRVEALNEAFDPHFHQ
AVQEVENTAVPNATIVSVLQDGYVIHDRLLRPAMVVVSRGGPKREPKPAADSADGGVNTTV
>Mature_220_residues
PQHNDTPESRTPQDSSAPASPAAAADDTTAAVAEAAEAIARADLEERITALEDDNRRLKEEYLRALAEAQNAKRMADKRI
EDNSRYAVSNFAKAVLGVADNLGRALLSVPEEARGGNEMVKNLAFGVELTAKELENALAQYQIRRVEALNEAFDPHFHQA
VQEVENTAVPNATIVSVLQDGYVIHDRLLRPAMVVVSRGGPKREPKPAADSADGGVNTTV

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=162, Percent_Identity=31.4814814814815, Blast_Score=84, Evalue=8e-17,
Organism=Homo sapiens, GI40255109, Length=150, Percent_Identity=27.3333333333333, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1788967, Length=143, Percent_Identity=30.7692307692308, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17552458, Length=152, Percent_Identity=34.2105263157895, Blast_Score=83, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6324806, Length=156, Percent_Identity=32.0512820512821, Blast_Score=91, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24653432, Length=155, Percent_Identity=33.5483870967742, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 23836; Mature: 23704

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQHNDTPESRTPQDSSAPASPAAAADDTTAAVAEAAEAIARADLEERITALEDDNRRLK
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
EEYLRALAEAQNAKRMADKRIEDNSRYAVSNFAKAVLGVADNLGRALLSVPEEARGGNEM
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHH
VKNLAFGVELTAKELENALAQYQIRRVEALNEAFDPHFHQAVQEVENTAVPNATIVSVLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHH
DGYVIHDRLLRPAMVVVSRGGPKREPKPAADSADGGVNTTV
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PQHNDTPESRTPQDSSAPASPAAAADDTTAAVAEAAEAIARADLEERITALEDDNRRLK
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
EEYLRALAEAQNAKRMADKRIEDNSRYAVSNFAKAVLGVADNLGRALLSVPEEARGGNEM
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHH
VKNLAFGVELTAKELENALAQYQIRRVEALNEAFDPHFHQAVQEVENTAVPNATIVSVLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHH
DGYVIHDRLLRPAMVVVSRGGPKREPKPAADSADGGVNTTV
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA