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The map label for this gene is rph

Identifier: 83594970

GI number: 83594970

Start: 4182078

End: 4182800

Strand: Reverse

Name: rph

Synonym: Rru_A3641

Alternate gene names: 83594970

Gene position: 4182800-4182078 (Counterclockwise)

Preceding gene: 83594973

Following gene: 83594969

Centisome position: 96.09

GC content: 67.22

Gene sequence:

>723_bases
ATGAGACCCTCCGGTCGCGCCGTCGACAGCCTGCGCTCCATCGTTCTCGAGCCGGGCTATTCCAAGCATGCCGAAGGATC
GTGCATGGCCCGCTTCGGCGATACCCATGTGCTGTGCACCGCCTCGATCGAGGAGCGGGTGCCGGGCTGGATGCGCGGCG
GCGGCAAGGGCTGGGTGACCGCCGAATACGGCATGCTGCCGCGCTCGACCCATACCCGCACCGACCGCGAGGCGGCGCGC
GGGCGCCAAAGCGGCCGCACCCAGGAAATTCAGCGCCTGATCGGCCGCTCGCTGCGCTCAGTCACCGACCTGAAAAAGAT
CGGCGAAATCCAGATCCGCCTGGATTGCGATGTCATCCAGGCCGATGGCGGCACCCGCACCGCCGCCATCACCGGATCGT
GGGTGGCGCTTTATCAGGCGCTGGCGCGCTTCGAGAAGCTGGGCCTTGGCGCCCTGCCGCCGCTGACCGACCATGTGGCG
GCGGTGTCTTGCGGCCTGTGCGATGGCGTGGCCGTGCTCGATCTTGATTACGCCGAGGACAGCACGGCCCAGGCCGATGC
CAATTTCGTTTTGACCGGTACCGGCGGCATCGTCGAGATCCAGGGCACGGCCGAAACCTCGCCCTTCGCCCAGGCCCAGT
TCATGGAGTTGCTGGCCCTGGCGCAAAAAGGCATCACCGAGTTGGTCGCCCTGCAGAAGGCGGCCCTCGCCAGCTTGCGC
TAA

Upstream 100 bases:

>100_bases
CGCTTCTCCCCATCGGCCGGGCGAAACCCTTGCGGCGGCCGGCGCGGACCTCTAGCTTGCCGGCAAACATCCCCCTTTCC
TCCTCGTAAGGACCATCTCC

Downstream 100 bases:

>100_bases
TTCTTTTCCGTTTCCCCCGATCAGGACCTGATGGCCATGTCGCGTCGCCTTGTTGAAAGTCCGCTGGTCGTTGCCAGCCA
TAACGCCGGAAAGGTCCGCG

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MRPSGRAVDSLRSIVLEPGYSKHAEGSCMARFGDTHVLCTASIEERVPGWMRGGGKGWVTAEYGMLPRSTHTRTDREAAR
GRQSGRTQEIQRLIGRSLRSVTDLKKIGEIQIRLDCDVIQADGGTRTAAITGSWVALYQALARFEKLGLGALPPLTDHVA
AVSCGLCDGVAVLDLDYAEDSTAQADANFVLTGTGGIVEIQGTAETSPFAQAQFMELLALAQKGITELVALQKAALASLR

Sequences:

>Translated_240_residues
MRPSGRAVDSLRSIVLEPGYSKHAEGSCMARFGDTHVLCTASIEERVPGWMRGGGKGWVTAEYGMLPRSTHTRTDREAAR
GRQSGRTQEIQRLIGRSLRSVTDLKKIGEIQIRLDCDVIQADGGTRTAAITGSWVALYQALARFEKLGLGALPPLTDHVA
AVSCGLCDGVAVLDLDYAEDSTAQADANFVLTGTGGIVEIQGTAETSPFAQAQFMELLALAQKGITELVALQKAALASLR
>Mature_240_residues
MRPSGRAVDSLRSIVLEPGYSKHAEGSCMARFGDTHVLCTASIEERVPGWMRGGGKGWVTAEYGMLPRSTHTRTDREAAR
GRQSGRTQEIQRLIGRSLRSVTDLKKIGEIQIRLDCDVIQADGGTRTAAITGSWVALYQALARFEKLGLGALPPLTDHVA
AVSCGLCDGVAVLDLDYAEDSTAQADANFVLTGTGGIVEIQGTAETSPFAQAQFMELLALAQKGITELVALQKAALASLR

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=59.2417061611374, Blast_Score=259, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI71981632, Length=186, Percent_Identity=27.9569892473118, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_RHORT (Q2RN60)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428722.1
- ProteinModelPortal:   Q2RN60
- SMR:   Q2RN60
- STRING:   Q2RN60
- GeneID:   3837097
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3641
- NMPDR:   fig|1085.1.peg.1275
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   Q2RN60
- ProtClustDB:   PRK00173
- BioCyc:   RRUB269796:RRU_A3641-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25601; Mature: 25601

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSGRAVDSLRSIVLEPGYSKHAEGSCMARFGDTHVLCTASIEERVPGWMRGGGKGWVT
CCCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEECCHHHHCCHHHCCCCCCEEE
AEYGMLPRSTHTRTDREAARGRQSGRTQEIQRLIGRSLRSVTDLKKIGEIQIRLDCDVIQ
EECCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTAAITGSWVALYQALARFEKLGLGALPPLTDHVAAVSCGLCDGVAVLDLDYAED
CCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCC
STAQADANFVLTGTGGIVEIQGTAETSPFAQAQFMELLALAQKGITELVALQKAALASLR
CCCCCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRPSGRAVDSLRSIVLEPGYSKHAEGSCMARFGDTHVLCTASIEERVPGWMRGGGKGWVT
CCCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEECCHHHHCCHHHCCCCCCEEE
AEYGMLPRSTHTRTDREAARGRQSGRTQEIQRLIGRSLRSVTDLKKIGEIQIRLDCDVIQ
EECCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTAAITGSWVALYQALARFEKLGLGALPPLTDHVAAVSCGLCDGVAVLDLDYAED
CCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCC
STAQADANFVLTGTGGIVEIQGTAETSPFAQAQFMELLALAQKGITELVALQKAALASLR
CCCCCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA