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The map label for this gene is rho [H]

Identifier: 83594948

GI number: 83594948

Start: 4157912

End: 4159168

Strand: Direct

Name: rho [H]

Synonym: Rru_A3619

Alternate gene names: 83594948

Gene position: 4157912-4159168 (Clockwise)

Preceding gene: 83594947

Following gene: 83594952

Centisome position: 95.52

GC content: 59.43

Gene sequence:

>1257_bases
ATGCACCTTCAGGAACTGAAAAGCAAATCTCCGGCCGATCTTTTGGCTTTCGCCGAAGAACTGGAGATCGAGAACGCGAG
CAACCTGCGCAAGCAGGACATGATGTTCGCGATCTTGAAGCAACTCGCCGAAAATGATACCGCAATCTTTGGCGAGGGGG
TTCTGGAAATTCTTCAGGACGGCTTCGGCTTCCTGCGGGCGCCCGAGTCCAATTACCTGCCCGGGCCCGATGATATTTAT
GTCTCGCCCAGTCAGGTCCGTCGCTTCGGTCTGCGCACCGGCGATACGGTCGAGGGCCAGATCCGCGCCCCCAAGGATGG
CGAGCGCTATTTCGCCCTGCTCAAGGTCAATAAGATCAACTTTGAGGCGCCGGAATCCGTCCGTCACCGCATCAATTTCG
ACAATCTGACGCCGCTCTATCCCGATGAGCGGTTGAAGCTGGAAATCGACGATCCGACCCGCAAGGAATTCACCACCCGG
ATCATCGATCTCATCACGCCGCTGGGCAAGGGCCAGCGCGCCCTGATCGTGGCGCCGCCGCGCACCGGTAAAACGGTGAT
GCTGCAGAACGTCGCCCATGCGATTTCGGCCAATAATCCGGAAGTCTATCTGATCGTGCTGCTCATCGACGAGCGGCCCG
AGGAAGTGACCGACATGGCTCGCTCGGTGAACGGCGAGGTGGTCAGTTCCACCTTCGACGAACCGGCCTCGCGCCACGTT
CAGGTCGCCGAGATGGTGATCGAAAAGGCCAAGCGCCTGGTCGAGCACAAACGCGACGTCGTCATCCTTCTTGATTCGAT
CACCCGTCTCGCCCGCGCCTATAACACCGTGGTGCCCAGTTCGGGCAAGGTGCTGACCGGTGGTGTCGACGCCAATGCCC
TGCAGCGCCCCAAGCGCTTCTTCGGCGCCGCCCGTAATATCGAAGAGGGCGGATCGCTGACCATCATCGCCACGGCGCTG
ATCGATACCGGATCGCGCATGGACGAGGTGATCTTCGAAGAGTTCAAGGGGACCGGTAACTCGGAAATCATCCTGGACCG
CAAGCTCGCCGACCGCCGCGTCTTCCCGTCGATCGATATCGCCCGCTCGGGCACCCGGAAGGAAGAACTTCTCGTCCGCA
AGGATGTGCTGTCGAAGATGTGGGTGCTGCGCCGGATCCTCTCGCCGATGGGCGTTCAGGACGCCATGGAATTCCTTCTT
GGCAAGATGAAGGAAACCAAGAACAACGACGATTTCTTCGACGCGATGAATTCCTGA

Upstream 100 bases:

>100_bases
TTCTGGCTATTTGACCGGGTTCTCGACCGGCTCCGCAAGCCGGGCATCATCGGCCTTCCGCTCGCTCTTCCACGATATCC
TCTCTGTTCGAGACGACTCC

Downstream 100 bases:

>100_bases
TTCAGGCGCTTATCGCTTTTAAAATTTGGGGCTCCCTGCAGACTGTCTGCAGGGGGCCCCTTTTTTTGTTTCACGTGAAA
CAGCGCTGCAGGCGCGAAGC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MHLQELKSKSPADLLAFAEELEIENASNLRKQDMMFAILKQLAENDTAIFGEGVLEILQDGFGFLRAPESNYLPGPDDIY
VSPSQVRRFGLRTGDTVEGQIRAPKDGERYFALLKVNKINFEAPESVRHRINFDNLTPLYPDERLKLEIDDPTRKEFTTR
IIDLITPLGKGQRALIVAPPRTGKTVMLQNVAHAISANNPEVYLIVLLIDERPEEVTDMARSVNGEVVSSTFDEPASRHV
QVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATAL
IDTGSRMDEVIFEEFKGTGNSEIILDRKLADRRVFPSIDIARSGTRKEELLVRKDVLSKMWVLRRILSPMGVQDAMEFLL
GKMKETKNNDDFFDAMNS

Sequences:

>Translated_418_residues
MHLQELKSKSPADLLAFAEELEIENASNLRKQDMMFAILKQLAENDTAIFGEGVLEILQDGFGFLRAPESNYLPGPDDIY
VSPSQVRRFGLRTGDTVEGQIRAPKDGERYFALLKVNKINFEAPESVRHRINFDNLTPLYPDERLKLEIDDPTRKEFTTR
IIDLITPLGKGQRALIVAPPRTGKTVMLQNVAHAISANNPEVYLIVLLIDERPEEVTDMARSVNGEVVSSTFDEPASRHV
QVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATAL
IDTGSRMDEVIFEEFKGTGNSEIILDRKLADRRVFPSIDIARSGTRKEELLVRKDVLSKMWVLRRILSPMGVQDAMEFLL
GKMKETKNNDDFFDAMNS
>Mature_418_residues
MHLQELKSKSPADLLAFAEELEIENASNLRKQDMMFAILKQLAENDTAIFGEGVLEILQDGFGFLRAPESNYLPGPDDIY
VSPSQVRRFGLRTGDTVEGQIRAPKDGERYFALLKVNKINFEAPESVRHRINFDNLTPLYPDERLKLEIDDPTRKEFTTR
IIDLITPLGKGQRALIVAPPRTGKTVMLQNVAHAISANNPEVYLIVLLIDERPEEVTDMARSVNGEVVSSTFDEPASRHV
QVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATAL
IDTGSRMDEVIFEEFKGTGNSEIILDRKLADRRVFPSIDIARSGTRKEELLVRKDVLSKMWVLRRILSPMGVQDAMEFLL
GKMKETKNNDDFFDAMNS

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=416, Percent_Identity=68.75, Blast_Score=607, Evalue=1e-175,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 46894; Mature: 46894

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLQELKSKSPADLLAFAEELEIENASNLRKQDMMFAILKQLAENDTAIFGEGVLEILQD
CCHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHC
GFGFLRAPESNYLPGPDDIYVSPSQVRRFGLRTGDTVEGQIRAPKDGERYFALLKVNKIN
CCCEEECCCCCCCCCCCCEEECHHHHHHHCCCCCCCCCCEEECCCCCCEEEEEEEEEECC
FEAPESVRHRINFDNLTPLYPDERLKLEIDDPTRKEFTTRIIDLITPLGKGQRALIVAPP
CCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEECC
RTGKTVMLQNVAHAISANNPEVYLIVLLIDERPEEVTDMARSVNGEVVSSTFDEPASRHV
CCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHCCHHHHHH
QVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNTVVPSSGKVLTGGVDANALQRPKRF
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHH
FGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNSEIILDRKLADRRVFPSIDI
HHHHCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCCEEEEECCHHCCCCCCCCCH
ARSGTRKEELLVRKDVLSKMWVLRRILSPMGVQDAMEFLLGKMKETKNNDDFFDAMNS
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCC
>Mature Secondary Structure
MHLQELKSKSPADLLAFAEELEIENASNLRKQDMMFAILKQLAENDTAIFGEGVLEILQD
CCHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHC
GFGFLRAPESNYLPGPDDIYVSPSQVRRFGLRTGDTVEGQIRAPKDGERYFALLKVNKIN
CCCEEECCCCCCCCCCCCEEECHHHHHHHCCCCCCCCCCEEECCCCCCEEEEEEEEEECC
FEAPESVRHRINFDNLTPLYPDERLKLEIDDPTRKEFTTRIIDLITPLGKGQRALIVAPP
CCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEECC
RTGKTVMLQNVAHAISANNPEVYLIVLLIDERPEEVTDMARSVNGEVVSSTFDEPASRHV
CCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHCCHHHHHH
QVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNTVVPSSGKVLTGGVDANALQRPKRF
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHH
FGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNSEIILDRKLADRRVFPSIDI
HHHHCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCCEEEEECCHHCCCCCCCCCH
ARSGTRKEELLVRKDVLSKMWVLRRILSPMGVQDAMEFLLGKMKETKNNDDFFDAMNS
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606169 [H]