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The map label for this gene is trpE(G) [H]

Identifier: 83594875

GI number: 83594875

Start: 4083456

End: 4085642

Strand: Direct

Name: trpE(G) [H]

Synonym: Rru_A3546

Alternate gene names: 83594875

Gene position: 4083456-4085642 (Clockwise)

Preceding gene: 83594874

Following gene: 83594876

Centisome position: 93.81

GC content: 71.19

Gene sequence:

>2187_bases
ATGCAAACATCGACCTTCACCACCGCCGGCGGTTTTACCATCAGCAGTTGCCTACGCCCGCTTGCCGGGGCGGATGGCGC
CTTCGAGGCGCTGATCGACCGACTCGATCGCCATCGCGGCATGGTGATCGCCTCGACCTATGAGTATCCCGGCCGCTATC
GCCGCCACGCCCTGGGGTTTTGCGATCCGCCGCTGGTGCTGGAGGGCAAGGGGCGCGAGGCCCATCTGCTGGCGCTCAAT
CGCCGGGGGCGGGCCCTGCTGCCGGCCCTGGCCGTTGGCTTGGAGGGGGCGGCCGGGCTGGAGGGCCTGCGCCGCGAGGC
CGACCGGCTGGTCTTGCGTGTCAAGGCCATGGCGCCCTGGTTCCCCGAGGAGGAGCGCTCGCGCCAACCCTCGCTGATGT
CGGTGGTGCGCGCCCTCGCCGGGCTGTTCGCCGCCGAAAACGACCCGTTTTTCGGGCTTGTCGGCGCCTTTGGCTATGAC
CTGGGCTTGGCTTTCGAGCGCCTGCCCCATGCCCGGCCGCGATCGGCCGATCACCGCGATCTGGTGCTTTACCTGCCCGA
CCGTCTGCTGATCGACGATCCCGAAGCCGGCGGCCTTGCCGAACGCCTTTACGACATCACCGCGGCCGATGGGGCAAGCA
CCGCCGGCTTGGCGCGGGAAACCGCCGCTTACACCGCCGACCACCCCGCCGGCGGCGTGCCGATCGAGGATGATATGCCC
CCGGGCGCTTACGGGGCGATCGTCCGTGGGCTGAAGGAGGCCTTCGCCGCCGGGGATCTGTTCGAGGCGGTGCCCTCGCG
CGCCCTGCGCCGGCCTTGCGCCGAGGCGCCGAGCCGTTTGTACCGGCGGCTGCGCGCGGCCAATCCGGCGCCCTATCTGT
TCCTGGCCAATCTGGGGGCGGGCGAACATCTGATCGGCGCCTCGCCCGAAATGTTCGTGCGCGTCGGTGGAGCGCCGGGG
GCGCGGCGGGTGGAAACCTGCCCGATCTCGGGCACCATCGCCCGGGGGCCCGACGCCCTGGGCGATGCCGAGGCCATCCG
CACCCTGCTCAATTCGACCAAGGACGAGGCCGAATTGACCATGTGCACCGATGTCGACCGCAACGACAAGGCGCGGGTCT
GCGTGGCCGGCAGCGTTACCGTCATCGGTCGCCGCCAGATCGAGCTATATTCCCGGCTGATCCACACGGTCGACCATGTG
GAGGGCCGGCTGCGCCCCGAGCTTGACGCCCTTGACGCCTTTCTCAGCCACTGCTGGGCGGTGACGGTGACCGGAGCGCC
CAAGCGCGCCGCCATGGCCGCCGTCGAAGCCGTGGAGCGGGCGCCGCGCGCCTGGTATGGCGGCGCCATCGGCCGCCTGG
GCTTCGACGGTACCCTCGACACCGGACTGGTGCTGCGCACCATCCGCCTGCGTCACGGCGTCGCCGAGGTGCGGGTGGGG
GCGACGCTGCTCCACCGTTCCGATCCCGAGGAGGAGGAGGCCGAAACCCTGCTCAAGGCCTCGGCCCTGCTCGCCCTGCT
CGATGCCACCACCCCGGCCAAGCCGAATGCCCCGCATCTTCCGTTGCGCGGCCGGGCGCCGCGCGTGCTGGTCATCGACC
ACGAGGACAGCTTCGTCCATACCCTGGCGTCTTATCTGCGCAATGCCGGGGCCGAGACCACCGTGCTGCGCTGGGACGTG
CCGGCGGCGGTGCGCGCCGGCGTCGAGGCCGATCTGCTGGTGCTGTCGCCGGGACCGGGCACGCCGTCGCGCTTCGCCCT
GGGGGCCAGCCTGGACTGGGCGGTGGCGCGCGGCTTGCCGGTCTTCGGCGTCTGCCTGGGGCTGCAGGGCATCGTCGAAC
AGGCCGGGGGCCGCCTTGCCCGGCTGGCGGTTCCCGCCCATGGCATGGCCTCGACGCTGCGGCTGGTCGCCCCCGGGGAC
CCGCTGTTCGCCGGCCTGCCCACGACCATGAGAGTGGGCCGCTACCACAGCCTGCACGCCGAGCGCGCCAGCCTGCCCGA
CAGCCTGGAGATCCTGGCCGAAAGCGACGACGGGGTGATCATGGCGCTGCGCCACCGCCTGCTGCCCTTCAGCGCCGTGC
AATTCCACCCCGAAAGCCTGATGACCCTGGACGGCGGCGCCGGACCCCGATTGATCGCCAATCTTCTGGAAACCCTAAGC
GTCCCGCGAACGCGCCACGCCGCCTAA

Upstream 100 bases:

>100_bases
CCCCGAAGCCCCGGTGTCGCGCCCTTTCCCCTGATCCCCACCGGTCGCCCCCCCTCCGTCTCCTCGCCAGAGTCCGCCGT
TGATCCAACGGAGAGGACCC

Downstream 100 bases:

>100_bases
GAGTGTTTGCCCGGACGCTTGTGCACCCTTACATGGAGGAGGGGGCGGGGGGTACAACGGGCCCGCCCCGCCCGTCCTGG
AGACGTCATGGAACCCAAAG

Product: anthranilate synthase

Products: NA

Alternate protein names: Glutamine amidotransferase [H]

Number of amino acids: Translated: 728; Mature: 728

Protein sequence:

>728_residues
MQTSTFTTAGGFTISSCLRPLAGADGAFEALIDRLDRHRGMVIASTYEYPGRYRRHALGFCDPPLVLEGKGREAHLLALN
RRGRALLPALAVGLEGAAGLEGLRREADRLVLRVKAMAPWFPEEERSRQPSLMSVVRALAGLFAAENDPFFGLVGAFGYD
LGLAFERLPHARPRSADHRDLVLYLPDRLLIDDPEAGGLAERLYDITAADGASTAGLARETAAYTADHPAGGVPIEDDMP
PGAYGAIVRGLKEAFAAGDLFEAVPSRALRRPCAEAPSRLYRRLRAANPAPYLFLANLGAGEHLIGASPEMFVRVGGAPG
ARRVETCPISGTIARGPDALGDAEAIRTLLNSTKDEAELTMCTDVDRNDKARVCVAGSVTVIGRRQIELYSRLIHTVDHV
EGRLRPELDALDAFLSHCWAVTVTGAPKRAAMAAVEAVERAPRAWYGGAIGRLGFDGTLDTGLVLRTIRLRHGVAEVRVG
ATLLHRSDPEEEEAETLLKASALLALLDATTPAKPNAPHLPLRGRAPRVLVIDHEDSFVHTLASYLRNAGAETTVLRWDV
PAAVRAGVEADLLVLSPGPGTPSRFALGASLDWAVARGLPVFGVCLGLQGIVEQAGGRLARLAVPAHGMASTLRLVAPGD
PLFAGLPTTMRVGRYHSLHAERASLPDSLEILAESDDGVIMALRHRLLPFSAVQFHPESLMTLDGGAGPRLIANLLETLS
VPRTRHAA

Sequences:

>Translated_728_residues
MQTSTFTTAGGFTISSCLRPLAGADGAFEALIDRLDRHRGMVIASTYEYPGRYRRHALGFCDPPLVLEGKGREAHLLALN
RRGRALLPALAVGLEGAAGLEGLRREADRLVLRVKAMAPWFPEEERSRQPSLMSVVRALAGLFAAENDPFFGLVGAFGYD
LGLAFERLPHARPRSADHRDLVLYLPDRLLIDDPEAGGLAERLYDITAADGASTAGLARETAAYTADHPAGGVPIEDDMP
PGAYGAIVRGLKEAFAAGDLFEAVPSRALRRPCAEAPSRLYRRLRAANPAPYLFLANLGAGEHLIGASPEMFVRVGGAPG
ARRVETCPISGTIARGPDALGDAEAIRTLLNSTKDEAELTMCTDVDRNDKARVCVAGSVTVIGRRQIELYSRLIHTVDHV
EGRLRPELDALDAFLSHCWAVTVTGAPKRAAMAAVEAVERAPRAWYGGAIGRLGFDGTLDTGLVLRTIRLRHGVAEVRVG
ATLLHRSDPEEEEAETLLKASALLALLDATTPAKPNAPHLPLRGRAPRVLVIDHEDSFVHTLASYLRNAGAETTVLRWDV
PAAVRAGVEADLLVLSPGPGTPSRFALGASLDWAVARGLPVFGVCLGLQGIVEQAGGRLARLAVPAHGMASTLRLVAPGD
PLFAGLPTTMRVGRYHSLHAERASLPDSLEILAESDDGVIMALRHRLLPFSAVQFHPESLMTLDGGAGPRLIANLLETLS
VPRTRHAA
>Mature_728_residues
MQTSTFTTAGGFTISSCLRPLAGADGAFEALIDRLDRHRGMVIASTYEYPGRYRRHALGFCDPPLVLEGKGREAHLLALN
RRGRALLPALAVGLEGAAGLEGLRREADRLVLRVKAMAPWFPEEERSRQPSLMSVVRALAGLFAAENDPFFGLVGAFGYD
LGLAFERLPHARPRSADHRDLVLYLPDRLLIDDPEAGGLAERLYDITAADGASTAGLARETAAYTADHPAGGVPIEDDMP
PGAYGAIVRGLKEAFAAGDLFEAVPSRALRRPCAEAPSRLYRRLRAANPAPYLFLANLGAGEHLIGASPEMFVRVGGAPG
ARRVETCPISGTIARGPDALGDAEAIRTLLNSTKDEAELTMCTDVDRNDKARVCVAGSVTVIGRRQIELYSRLIHTVDHV
EGRLRPELDALDAFLSHCWAVTVTGAPKRAAMAAVEAVERAPRAWYGGAIGRLGFDGTLDTGLVLRTIRLRHGVAEVRVG
ATLLHRSDPEEEEAETLLKASALLALLDATTPAKPNAPHLPLRGRAPRVLVIDHEDSFVHTLASYLRNAGAETTVLRWDV
PAAVRAGVEADLLVLSPGPGTPSRFALGASLDWAVARGLPVFGVCLGLQGIVEQAGGRLARLAVPAHGMASTLRLVAPGD
PLFAGLPTTMRVGRYHSLHAERASLPDSLEILAESDDGVIMALRHRLLPFSAVQFHPESLMTLDGGAGPRLIANLLETLS
VPRTRHAA

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=415, Percent_Identity=33.0120481927711, Blast_Score=169, Evalue=6e-43,
Organism=Escherichia coli, GI1788114, Length=367, Percent_Identity=29.700272479564, Blast_Score=133, Evalue=5e-32,
Organism=Escherichia coli, GI1789760, Length=192, Percent_Identity=37.5, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1787517, Length=181, Percent_Identity=37.5690607734807, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI1786809, Length=233, Percent_Identity=30.4721030042918, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320935, Length=269, Percent_Identity=36.8029739776952, Blast_Score=160, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6322638, Length=191, Percent_Identity=36.1256544502618, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324361, Length=123, Percent_Identity=30.8943089430894, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR006805
- InterPro:   IPR006220
- InterPro:   IPR010112
- InterPro:   IPR015890
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind; PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 77755; Mature: 77755

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTSTFTTAGGFTISSCLRPLAGADGAFEALIDRLDRHRGMVIASTYEYPGRYRRHALGF
CCCCCEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHCCC
CDPPLVLEGKGREAHLLALNRRGRALLPALAVGLEGAAGLEGLRREADRLVLRVKAMAPW
CCCCEEEECCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHEEHHHHCCC
FPEEERSRQPSLMSVVRALAGLFAAENDPFFGLVGAFGYDLGLAFERLPHARPRSADHRD
CCCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCE
LVLYLPDRLLIDDPEAGGLAERLYDITAADGASTAGLARETAAYTADHPAGGVPIEDDMP
EEEEECCEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
PGAYGAIVRGLKEAFAAGDLFEAVPSRALRRPCAEAPSRLYRRLRAANPAPYLFLANLGA
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEECCC
GEHLIGASPEMFVRVGGAPGARRVETCPISGTIARGPDALGDAEAIRTLLNSTKDEAELT
CCCCCCCCCCEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEE
MCTDVDRNDKARVCVAGSVTVIGRRQIELYSRLIHTVDHVEGRLRPELDALDAFLSHCWA
EEECCCCCCCCEEEEECCEEEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEE
VTVTGAPKRAAMAAVEAVERAPRAWYGGAIGRLGFDGTLDTGLVLRTIRLRHGVAEVRVG
EEECCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHH
ATLLHRSDPEEEEAETLLKASALLALLDATTPAKPNAPHLPLRGRAPRVLVIDHEDSFVH
HHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHH
TLASYLRNAGAETTVLRWDVPAAVRAGVEADLLVLSPGPGTPSRFALGASLDWAVARGLP
HHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEECCCCCCCCCEEECCCCHHHHHCCCH
VFGVCLGLQGIVEQAGGRLARLAVPAHGMASTLRLVAPGDPLFAGLPTTMRVGRYHSLHA
HHHHHHHHHHHHHHCCCCEEEEECCCCCCHHEEEEECCCCCCEECCCHHHHHCCHHHHHH
ERASLPDSLEILAESDDGVIMALRHRLLPFSAVQFHPESLMTLDGGAGPRLIANLLETLS
HHHCCCCCHHEEECCCCCEEHHHHHHHCCCHHEEECCCCEEEECCCCCHHHHHHHHHHHC
VPRTRHAA
CCCCCCCC
>Mature Secondary Structure
MQTSTFTTAGGFTISSCLRPLAGADGAFEALIDRLDRHRGMVIASTYEYPGRYRRHALGF
CCCCCEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHCCC
CDPPLVLEGKGREAHLLALNRRGRALLPALAVGLEGAAGLEGLRREADRLVLRVKAMAPW
CCCCEEEECCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHEEHHHHCCC
FPEEERSRQPSLMSVVRALAGLFAAENDPFFGLVGAFGYDLGLAFERLPHARPRSADHRD
CCCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCE
LVLYLPDRLLIDDPEAGGLAERLYDITAADGASTAGLARETAAYTADHPAGGVPIEDDMP
EEEEECCEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
PGAYGAIVRGLKEAFAAGDLFEAVPSRALRRPCAEAPSRLYRRLRAANPAPYLFLANLGA
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEECCC
GEHLIGASPEMFVRVGGAPGARRVETCPISGTIARGPDALGDAEAIRTLLNSTKDEAELT
CCCCCCCCCCEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEE
MCTDVDRNDKARVCVAGSVTVIGRRQIELYSRLIHTVDHVEGRLRPELDALDAFLSHCWA
EEECCCCCCCCEEEEECCEEEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEE
VTVTGAPKRAAMAAVEAVERAPRAWYGGAIGRLGFDGTLDTGLVLRTIRLRHGVAEVRVG
EEECCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHH
ATLLHRSDPEEEEAETLLKASALLALLDATTPAKPNAPHLPLRGRAPRVLVIDHEDSFVH
HHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHH
TLASYLRNAGAETTVLRWDVPAAVRAGVEADLLVLSPGPGTPSRFALGASLDWAVARGLP
HHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEECCCCCCCCCEEECCCCHHHHHCCCH
VFGVCLGLQGIVEQAGGRLARLAVPAHGMASTLRLVAPGDPLFAGLPTTMRVGRYHSLHA
HHHHHHHHHHHHHHCCCCEEEEECCCCCCHHEEEEECCCCCCEECCCHHHHHCCHHHHHH
ERASLPDSLEILAESDDGVIMALRHRLLPFSAVQFHPESLMTLDGGAGPRLIANLLETLS
HHHCCCCCHHEEECCCCCEEHHHHHHHCCCHHEEECCCCEEEECCCCCHHHHHHHHHHHC
VPRTRHAA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8939798 [H]