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The map label for this gene is pheS

Identifier: 83594852

GI number: 83594852

Start: 4054235

End: 4055311

Strand: Direct

Name: pheS

Synonym: Rru_A3523

Alternate gene names: 83594852

Gene position: 4054235-4055311 (Clockwise)

Preceding gene: 83594851

Following gene: 83594853

Centisome position: 93.14

GC content: 65.65

Gene sequence:

>1077_bases
ATGGATAATGTGGAAGACCTGCGCGGCACCCTGCTTTCCGCCGTCGCCGAGGCCGGGGATCTGGACGCGCTCGACGCCAT
CCGGGTTTCCGCCCTGGGCAAGAAGGGCCAGATCACCGGGCTGATGAAAACCCTGGGCGCCATGGATCCCGAGGCCCGCA
AGGCCGCCGGTCAGGCGCTCAATCTGGTCAAGGACGAGATCGCCGCCGCCCTTGACGCCCGCAAGGCCGATCTCGCCGCC
GCCGCCCTTGAAGCCAAGCTGGCCCGCGAGCGCCTTGATGTCTCGCTGGCCCCGGCCCCCGAGGCGACCGGCGCCATCCA
TCCGATCAGCCAGACCTGGGAAGAGGTGGTCGCTATCTTCGCCCAGATGGGCTTCGAGGTGGCCGAGGGCCCCGAGATCG
AGGATGATTTCCATAATTTCACGGCGCTGAATTTTCCGCCCGGCCATCCGGCGCGCGCCATGCACGACACCTTTTTCCTG
CCGACCCGCGAAGACGGCAGCCGCCACCTGCTGCGCACCCATACCTCGCCGGTGCAGATCCGCACGATGATGGGCCGCAA
GCCGCCGATCCGCATCCTGGCGCCCGGCCGTACCTACCGCTGCGATTCCGACATGACGCACACGCCGATGTTCCACCAGT
TCGAAGGGCTGGTCATCGACAAGGCGACCCATTTCGGCCATTTGAAAGGCTGTCTGCACGAATTCGTCCGCGCCTATTTC
GAGGTCGACGATCTGCCGATGCGCTTCCGCCCCAGCTTCTTCCCCTTCACCGAGCCCTCGGCCGAGGTCGATATCGGCTG
TTCGCGCAAGGGCGGCGCGTTGAAGATCGGCGCGGGCGACTCGTGGCTGGAGATTCTCGGCTGCGGCATGGTCCATCCCA
ATGTGCTGACCGCCTGCGGCCTTGATCCCGAGGAGTATCAGGGCTTCGCCTTCGGCATGGGCCTGGAGCGCATCGCCATG
CTGAAATACGGCATCCCCGATCTGCGGACCTTCTTCGAGTCCGATCTGCGCTGGCTGCGCCATTACGGCTTCGCCGCGCT
TGATCTTCCCACCCTGCACGGAGGGCTGTCGCGATGA

Upstream 100 bases:

>100_bases
GGGCGGCCCCCGTTTTTCTTGCGCCCGCGTCCGGCGGCCGTTCCCCGGGAGCGGACCATCGGGGGTGGGACGGCACCAGG
ACAGGACCAGGACAGACGTC

Downstream 100 bases:

>100_bases
AGTTCACCATCGGCTGGCTCAAGGACCACCTCGACACCCAGGCCAGCGTGGCCGATATCGCCGAAAAGCTGACGGCGCTC
GGCCTGGAGGTCGACGCCAT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MDNVEDLRGTLLSAVAEAGDLDALDAIRVSALGKKGQITGLMKTLGAMDPEARKAAGQALNLVKDEIAAALDARKADLAA
AALEAKLARERLDVSLAPAPEATGAIHPISQTWEEVVAIFAQMGFEVAEGPEIEDDFHNFTALNFPPGHPARAMHDTFFL
PTREDGSRHLLRTHTSPVQIRTMMGRKPPIRILAPGRTYRCDSDMTHTPMFHQFEGLVIDKATHFGHLKGCLHEFVRAYF
EVDDLPMRFRPSFFPFTEPSAEVDIGCSRKGGALKIGAGDSWLEILGCGMVHPNVLTACGLDPEEYQGFAFGMGLERIAM
LKYGIPDLRTFFESDLRWLRHYGFAALDLPTLHGGLSR

Sequences:

>Translated_358_residues
MDNVEDLRGTLLSAVAEAGDLDALDAIRVSALGKKGQITGLMKTLGAMDPEARKAAGQALNLVKDEIAAALDARKADLAA
AALEAKLARERLDVSLAPAPEATGAIHPISQTWEEVVAIFAQMGFEVAEGPEIEDDFHNFTALNFPPGHPARAMHDTFFL
PTREDGSRHLLRTHTSPVQIRTMMGRKPPIRILAPGRTYRCDSDMTHTPMFHQFEGLVIDKATHFGHLKGCLHEFVRAYF
EVDDLPMRFRPSFFPFTEPSAEVDIGCSRKGGALKIGAGDSWLEILGCGMVHPNVLTACGLDPEEYQGFAFGMGLERIAM
LKYGIPDLRTFFESDLRWLRHYGFAALDLPTLHGGLSR
>Mature_358_residues
MDNVEDLRGTLLSAVAEAGDLDALDAIRVSALGKKGQITGLMKTLGAMDPEARKAAGQALNLVKDEIAAALDARKADLAA
AALEAKLARERLDVSLAPAPEATGAIHPISQTWEEVVAIFAQMGFEVAEGPEIEDDFHNFTALNFPPGHPARAMHDTFFL
PTREDGSRHLLRTHTSPVQIRTMMGRKPPIRILAPGRTYRCDSDMTHTPMFHQFEGLVIDKATHFGHLKGCLHEFVRAYF
EVDDLPMRFRPSFFPFTEPSAEVDIGCSRKGGALKIGAGDSWLEILGCGMVHPNVLTACGLDPEEYQGFAFGMGLERIAM
LKYGIPDLRTFFESDLRWLRHYGFAALDLPTLHGGLSR

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=268, Percent_Identity=33.5820895522388, Blast_Score=128, Evalue=7e-30,
Organism=Homo sapiens, GI5729820, Length=258, Percent_Identity=28.2945736434109, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1788007, Length=342, Percent_Identity=54.3859649122807, Blast_Score=383, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32563657, Length=282, Percent_Identity=31.5602836879433, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17508957, Length=282, Percent_Identity=31.5602836879433, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32566635, Length=262, Percent_Identity=29.3893129770992, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321087, Length=374, Percent_Identity=27.5401069518717, Blast_Score=123, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6325304, Length=100, Percent_Identity=37, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI18858079, Length=310, Percent_Identity=30.9677419354839, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI17137424, Length=263, Percent_Identity=29.6577946768061, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_RHORT (Q2RNH8)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428604.1
- ProteinModelPortal:   Q2RNH8
- SMR:   Q2RNH8
- STRING:   Q2RNH8
- GeneID:   3836977
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3523
- NMPDR:   fig|1085.1.peg.1975
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   Q2RNH8
- ProtClustDB:   PRK00488
- BioCyc:   RRUB269796:RRU_A3523-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 39265; Mature: 39265

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNVEDLRGTLLSAVAEAGDLDALDAIRVSALGKKGQITGLMKTLGAMDPEARKAAGQAL
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
NLVKDEIAAALDARKADLAAAALEAKLARERLDVSLAPAPEATGAIHPISQTWEEVVAIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCHHHHHHHHHHHH
AQMGFEVAEGPEIEDDFHNFTALNFPPGHPARAMHDTFFLPTREDGSRHLLRTHTSPVQI
HHHCCCCCCCCCCCHHHCCCEEECCCCCCCCHHHHCEEECCCCCCCCCHHHHCCCCCEEH
RTMMGRKPPIRILAPGRTYRCDSDMTHTPMFHQFEGLVIDKATHFGHLKGCLHEFVRAYF
HHHHCCCCCEEEECCCCCEECCCCCCCCCHHHHHCCEEEECHHHHHHHHHHHHHHHHHHH
EVDDLPMRFRPSFFPFTEPSAEVDIGCSRKGGALKIGAGDSWLEILGCGMVHPNVLTACG
CCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEEECCCCHHHHHHHCCCCCCCHHHHCC
LDPEEYQGFAFGMGLERIAMLKYGIPDLRTFFESDLRWLRHYGFAALDLPTLHGGLSR
CCHHHHCCCHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCCC
>Mature Secondary Structure
MDNVEDLRGTLLSAVAEAGDLDALDAIRVSALGKKGQITGLMKTLGAMDPEARKAAGQAL
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
NLVKDEIAAALDARKADLAAAALEAKLARERLDVSLAPAPEATGAIHPISQTWEEVVAIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCHHHHHHHHHHHH
AQMGFEVAEGPEIEDDFHNFTALNFPPGHPARAMHDTFFLPTREDGSRHLLRTHTSPVQI
HHHCCCCCCCCCCCHHHCCCEEECCCCCCCCHHHHCEEECCCCCCCCCHHHHCCCCCEEH
RTMMGRKPPIRILAPGRTYRCDSDMTHTPMFHQFEGLVIDKATHFGHLKGCLHEFVRAYF
HHHHCCCCCEEEECCCCCEECCCCCCCCCHHHHHCCEEEECHHHHHHHHHHHHHHHHHHH
EVDDLPMRFRPSFFPFTEPSAEVDIGCSRKGGALKIGAGDSWLEILGCGMVHPNVLTACG
CCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEEECCCCHHHHHHHCCCCCCCHHHHCC
LDPEEYQGFAFGMGLERIAMLKYGIPDLRTFFESDLRWLRHYGFAALDLPTLHGGLSR
CCHHHHCCCHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA