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The map label for this gene is rna [H]

Identifier: 83594779

GI number: 83594779

Start: 3973519

End: 3974589

Strand: Direct

Name: rna [H]

Synonym: Rru_A3450

Alternate gene names: 83594779

Gene position: 3973519-3974589 (Clockwise)

Preceding gene: 83594768

Following gene: 83594780

Centisome position: 91.29

GC content: 71.8

Gene sequence:

>1071_bases
ATGATCATGGGATTGCTTGGCAGGGGTTTGCTCGGCCGGGGTTTTCTTGGCCGTTTTCTTGGGGCGGGGTGGCTGCTGGC
CGCGCCCGCCCTCTCTCCCGCCCTGGCCGAAGAGCCGCTTGTGGGGAGCTTCCTTGCCGAACGCGCCTGCCCGGCCTTTG
TCTCCTTGCGCAAGCAAACCAATCCCGGCGAGGTCCGCCTTGAGGTCGGGCGGGCCTACCCGGTGACGGCGGCCAATCGC
GCGGCAGCCACCCATGTTCTGCTCACCGTCGCCGAAGCCACCCCGACGCGGCGCTGGGCGGCCCTGGACTGCGGACAATT
GGCGGTGGAAGCCGGCGCGGCCCCGTCTTCGGCCCCCTCTTCGGCCCCCTCTTCGACCCCGTCTTCGGCCGCGTCTTCGA
CCTCGGCCTTCGTTCTGGCCCTGAGCTGGCAACCGGCCTTTTGCGAAGGCCGGCCGACCAAGCCCGAATGCGCCAGCCAG
ACCGCCGATCGCCTTGATGCCGATCACTTCGCCCTGCACGGCCTATGGCCCCAGCCGCGCGGCCGGGCCTATTGCGGTGT
CGATGCGGCGGCGGTCGCCCTGGATAAGGAGGGCGCCTGGGACCGCCTGCCCGCCGTAGCGCTGAGCGAGCCGACGCGCC
GGGCCCTTGTCCGGGCGATGCCCGGCACCGCCTCGTTCCTCGATCGCCACGAATGGATCAAGCATGGCACCTGCGCCGAC
GCCGGGGCCGGGCCGGGGGCCGAGGCCTATTACGCCGCCTCCCTCGCCCTGCTTGAGGCGATTAACGCCTCGCCGGTGCG
CGCCCTGGTCGCCGGGCGGATCGGCGGCCGGCTGGAGACGGCGGACTTGCGTCAGGCCTTCGACGCCGCTTTCGGAGCCG
GGGCCGGTCAGCGGGTCAGCGTGTCTTGCGTCCGCGATGGCGGGCGATCGCTGATCGGCGAGATCAAGCTAGCCCTGGCC
GGTCCCCTTGGCCCGGAGGTGGCCGGAGTTTTGGCGGCGCGCATCGCCGCCGTTCCCCCGGTCGATCCGGGCTGCCCCGG
CGGCATCGTCGATGCGGTGGGGCAGCAGTGA

Upstream 100 bases:

>100_bases
CCGGGGTCAAGGGACGGGAAGTCTTTGAAAAGATCCCCGGCGACGGGTATTGGGGGAGGCTAAGATCGCGCCGGCACCGG
CGAAGCGAGCGAGGGGAATG

Downstream 100 bases:

>100_bases
GCGGCTTGCGGCCGATCCCGGGCGCCGCGGACCCCAAGGCGACGACGCTGTGGACGGTTTGCTTGCCCACCCGGAAAACC
GCCCATCCCCTAAAAAGCCG

Product: ribonuclease T2

Products: NA

Alternate protein names: RNase I; Enterobacter ribonuclease [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MIMGLLGRGLLGRGFLGRFLGAGWLLAAPALSPALAEEPLVGSFLAERACPAFVSLRKQTNPGEVRLEVGRAYPVTAANR
AAATHVLLTVAEATPTRRWAALDCGQLAVEAGAAPSSAPSSAPSSTPSSAASSTSAFVLALSWQPAFCEGRPTKPECASQ
TADRLDADHFALHGLWPQPRGRAYCGVDAAAVALDKEGAWDRLPAVALSEPTRRALVRAMPGTASFLDRHEWIKHGTCAD
AGAGPGAEAYYAASLALLEAINASPVRALVAGRIGGRLETADLRQAFDAAFGAGAGQRVSVSCVRDGGRSLIGEIKLALA
GPLGPEVAGVLAARIAAVPPVDPGCPGGIVDAVGQQ

Sequences:

>Translated_356_residues
MIMGLLGRGLLGRGFLGRFLGAGWLLAAPALSPALAEEPLVGSFLAERACPAFVSLRKQTNPGEVRLEVGRAYPVTAANR
AAATHVLLTVAEATPTRRWAALDCGQLAVEAGAAPSSAPSSAPSSTPSSAASSTSAFVLALSWQPAFCEGRPTKPECASQ
TADRLDADHFALHGLWPQPRGRAYCGVDAAAVALDKEGAWDRLPAVALSEPTRRALVRAMPGTASFLDRHEWIKHGTCAD
AGAGPGAEAYYAASLALLEAINASPVRALVAGRIGGRLETADLRQAFDAAFGAGAGQRVSVSCVRDGGRSLIGEIKLALA
GPLGPEVAGVLAARIAAVPPVDPGCPGGIVDAVGQQ
>Mature_356_residues
MIMGLLGRGLLGRGFLGRFLGAGWLLAAPALSPALAEEPLVGSFLAERACPAFVSLRKQTNPGEVRLEVGRAYPVTAANR
AAATHVLLTVAEATPTRRWAALDCGQLAVEAGAAPSSAPSSAPSSTPSSAASSTSAFVLALSWQPAFCEGRPTKPECASQ
TADRLDADHFALHGLWPQPRGRAYCGVDAAAVALDKEGAWDRLPAVALSEPTRRALVRAMPGTASFLDRHEWIKHGTCAD
AGAGPGAEAYYAASLALLEAINASPVRALVAGRIGGRLETADLRQAFDAAFGAGAGQRVSVSCVRDGGRSLIGEIKLALA
GPLGPEVAGVLAARIAAVPPVDPGCPGGIVDAVGQQ

Specific function: One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid [H]

COG id: COG3719

COG function: function code J; Ribonuclease I

Gene ontology:

Cell location: Periplasm. Cytoplasm. Note=RNase I (periplasmic) and RNase I* (cytoplasmic) appear to be isoforms apparently encoded by the same gene. The cytoplasmic form is less active towards natural polymer RNA [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase T2 family [H]

Homologues:

Organism=Escherichia coli, GI1786828, Length=212, Percent_Identity=29.2452830188679, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001568
- InterPro:   IPR018188 [H]

Pfam domain/function: PF00445 Ribonuclease_T2 [H]

EC number: =3.1.27.6 [H]

Molecular weight: Translated: 36334; Mature: 36334

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS00530 RNASE_T2_1 ; PS00531 RNASE_T2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMGLLGRGLLGRGFLGRFLGAGWLLAAPALSPALAEEPLVGSFLAERACPAFVSLRKQT
CCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHCCC
NPGEVRLEVGRAYPVTAANRAAATHVLLTVAEATPTRRWAALDCGQLAVEAGAAPSSAPS
CCCEEEEEECCCCCCCCCCHHHHHHHEEEEECCCCCCCEECCCHHHHHHHCCCCCCCCCC
SAPSSTPSSAASSTSAFVLALSWQPAFCEGRPTKPECASQTADRLDADHFALHGLWPQPR
CCCCCCCCHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCC
GRAYCGVDAAAVALDKEGAWDRLPAVALSEPTRRALVRAMPGTASFLDRHEWIKHGTCAD
CCEEECCCHHHEEECCCCCHHHCCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
AGAGPGAEAYYAASLALLEAINASPVRALVAGRIGGRLETADLRQAFDAAFGAGAGQRVS
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEE
VSCVRDGGRSLIGEIKLALAGPLGPEVAGVLAARIAAVPPVDPGCPGGIVDAVGQQ
EEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure
MIMGLLGRGLLGRGFLGRFLGAGWLLAAPALSPALAEEPLVGSFLAERACPAFVSLRKQT
CCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHCCC
NPGEVRLEVGRAYPVTAANRAAATHVLLTVAEATPTRRWAALDCGQLAVEAGAAPSSAPS
CCCEEEEEECCCCCCCCCCHHHHHHHEEEEECCCCCCCEECCCHHHHHHHCCCCCCCCCC
SAPSSTPSSAASSTSAFVLALSWQPAFCEGRPTKPECASQTADRLDADHFALHGLWPQPR
CCCCCCCCHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCC
GRAYCGVDAAAVALDKEGAWDRLPAVALSEPTRRALVRAMPGTASFLDRHEWIKHGTCAD
CCEEECCCHHHEEECCCCCHHHCCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
AGAGPGAEAYYAASLALLEAINASPVRALVAGRIGGRLETADLRQAFDAAFGAGAGQRVS
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEE
VSCVRDGGRSLIGEIKLALAGPLGPEVAGVLAARIAAVPPVDPGCPGGIVDAVGQQ
EEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2253883; 8905232; 9278503; 8188249 [H]