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The map label for this gene is folC [H]

Identifier: 83594760

GI number: 83594760

Start: 3948781

End: 3950088

Strand: Direct

Name: folC [H]

Synonym: Rru_A3431

Alternate gene names: 83594760

Gene position: 3948781-3950088 (Clockwise)

Preceding gene: 83594759

Following gene: 83594768

Centisome position: 90.72

GC content: 72.02

Gene sequence:

>1308_bases
ATGAACGCTCCCGATGGGGTGCTCGCCCGCTTGATGGGCCTGCACCCCAAGGTGATCGACCTGTCGCTCGGCCGCGTCGA
GCGGTTGCTGGCCGCCCTGGGCCACCCCGAACGGGCCCTGCCGCCGGTCATCCATGTCGCGGGCACCAATGGCAAGGGAT
CGGTGGTCGCCACCTGCCGGGCCCTTCTGGAGGCGGCGGGCTACCGGGTCCATGTCTATACCAGCCCCCATTTGGTGCGC
TTTGGCGAACGCATCCGGCTGGCCGGCACCCTGATCGCCGACGAGGCGCTCCGTCGGCTGCTTGAAGAGGTGGAAAGCGC
CAATGCCAAGGCGCCGATCACCTTTTTCGAGGTGACGACGGCGGCGGCCTTCCTGGCCTTTTCGCGAAGCCCCGCCGATG
TGACCTTGCTCGAGGTCGGCCTGGGCGGGCGCCTTGACGCCACCAATGTCATCGCCGATCCCGCCGTTTGCGCGGTGACG
CCGATCGATCTTGATCATCAGCAGTATCTGGGATCAACGCTGACGGCCATCGCCGGGGAAAAGGCCGGGATTCTCAAGGC
CGGAGCCCCCGCCGTCATCGCCCCCCAACCGCCCGAGGCCGCCCAAACCCTCGCCGCCCGCGCCACCGCCGTCGGCGCGC
CGACCCTCTGGGCCGGGCGCGACTGGTCGTGGGAGCGCACGACGACGGGCTGGCGTTTCGCCGGGCTCGATCTGCCGCTC
CCCGTGCTTGCCGGTCCCCATCAACTGGGCAACGCCGCCCAGGCCCTGGCGGTGATCGACGCCCTGGAGGCACGGACCGG
CCTGCGGGTCGAGGGCGTGGCGCGGGCCAGGGGGCTGCGCGCCGTCGACTGGCCGGCCCGCCTGCAACGGTTAGACAGCG
GCCCCTTGGTCGAGAGACTGGCCCCGGGAACCACCTTGTGGCTGGACGGTGGCCATAATCCCCATGCCGGCCGCGCCCTG
GCCGCCGCCCTGGGCGACGATGCCAGCGGCCCGCTTGACGTGATTTGCGGCATGCTGGACACCAAGGACGCCGGGGGCTT
CCTTGGCGCCCTGGCGCCGCTGGTGCGGCGTTTGCGGTGCGTGGCCGTTCCCGATGGCCAGACCGGCGCCGGCCTGGCCC
CCGATGCCCTGGCCGGCCACGCCCGCGCCGCCGGCATCGCCGATGCCCGTTCCTGCGCCTCGGTAGCGGAGGCGCTGACC
GACCTCGGCGCGGGCACGACACCGCCCCCCGCCGCCCGCATCCTGATTTGCGGCTCGCTCTATCTGGCCGGCACCGTGCT
CGCCGAAAACGGCACTCCGCCGCGCTGA

Upstream 100 bases:

>100_bases
GAGGCGGTGGCCCGCAAGGTCGATGACGCCGACTTCGACGGGGCGGACGCCCCGCCACCGCCCGCGCCCAAGCCGGCGAA
GCCTTAGGCGGACCGTTGGC

Downstream 100 bases:

>100_bases
GGGGTCATCCCTCGACCACGCCCAAGACTCGCAGCCAGGGCAGGATATCGGTGATCAGGGGGGAAAGGCGGTCGGTCAGG
GCCTGGGTGGTGGCCGGGCC

Product: folylpolyglutamate synthetase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MNAPDGVLARLMGLHPKVIDLSLGRVERLLAALGHPERALPPVIHVAGTNGKGSVVATCRALLEAAGYRVHVYTSPHLVR
FGERIRLAGTLIADEALRRLLEEVESANAKAPITFFEVTTAAAFLAFSRSPADVTLLEVGLGGRLDATNVIADPAVCAVT
PIDLDHQQYLGSTLTAIAGEKAGILKAGAPAVIAPQPPEAAQTLAARATAVGAPTLWAGRDWSWERTTTGWRFAGLDLPL
PVLAGPHQLGNAAQALAVIDALEARTGLRVEGVARARGLRAVDWPARLQRLDSGPLVERLAPGTTLWLDGGHNPHAGRAL
AAALGDDASGPLDVICGMLDTKDAGGFLGALAPLVRRLRCVAVPDGQTGAGLAPDALAGHARAAGIADARSCASVAEALT
DLGAGTTPPPAARILICGSLYLAGTVLAENGTPPR

Sequences:

>Translated_435_residues
MNAPDGVLARLMGLHPKVIDLSLGRVERLLAALGHPERALPPVIHVAGTNGKGSVVATCRALLEAAGYRVHVYTSPHLVR
FGERIRLAGTLIADEALRRLLEEVESANAKAPITFFEVTTAAAFLAFSRSPADVTLLEVGLGGRLDATNVIADPAVCAVT
PIDLDHQQYLGSTLTAIAGEKAGILKAGAPAVIAPQPPEAAQTLAARATAVGAPTLWAGRDWSWERTTTGWRFAGLDLPL
PVLAGPHQLGNAAQALAVIDALEARTGLRVEGVARARGLRAVDWPARLQRLDSGPLVERLAPGTTLWLDGGHNPHAGRAL
AAALGDDASGPLDVICGMLDTKDAGGFLGALAPLVRRLRCVAVPDGQTGAGLAPDALAGHARAAGIADARSCASVAEALT
DLGAGTTPPPAARILICGSLYLAGTVLAENGTPPR
>Mature_435_residues
MNAPDGVLARLMGLHPKVIDLSLGRVERLLAALGHPERALPPVIHVAGTNGKGSVVATCRALLEAAGYRVHVYTSPHLVR
FGERIRLAGTLIADEALRRLLEEVESANAKAPITFFEVTTAAAFLAFSRSPADVTLLEVGLGGRLDATNVIADPAVCAVT
PIDLDHQQYLGSTLTAIAGEKAGILKAGAPAVIAPQPPEAAQTLAARATAVGAPTLWAGRDWSWERTTTGWRFAGLDLPL
PVLAGPHQLGNAAQALAVIDALEARTGLRVEGVARARGLRAVDWPARLQRLDSGPLVERLAPGTTLWLDGGHNPHAGRAL
AAALGDDASGPLDVICGMLDTKDAGGFLGALAPLVRRLRCVAVPDGQTGAGLAPDALAGHARAAGIADARSCASVAEALT
DLGAGTTPPPAARILICGSLYLAGTVLAENGTPPR

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=306, Percent_Identity=32.3529411764706, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI66932984, Length=306, Percent_Identity=32.3529411764706, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1788654, Length=430, Percent_Identity=35.3488372093023, Blast_Score=197, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI17553148, Length=296, Percent_Identity=31.7567567567568, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI71984923, Length=296, Percent_Identity=31.7567567567568, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17553150, Length=296, Percent_Identity=31.7567567567568, Blast_Score=148, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6323760, Length=428, Percent_Identity=30.3738317757009, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6324815, Length=315, Percent_Identity=29.8412698412698, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6322718, Length=287, Percent_Identity=28.2229965156794, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24641571, Length=212, Percent_Identity=32.5471698113208, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24581568, Length=198, Percent_Identity=31.8181818181818, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 44709; Mature: 44709

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPDGVLARLMGLHPKVIDLSLGRVERLLAALGHPERALPPVIHVAGTNGKGSVVATCR
CCCCHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHH
ALLEAAGYRVHVYTSPHLVRFGERIRLAGTLIADEALRRLLEEVESANAKAPITFFEVTT
HHHHHCCCEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHH
AAAFLAFSRSPADVTLLEVGLGGRLDATNVIADPAVCAVTPIDLDHQQYLGSTLTAIAGE
HHHHHHHCCCCCCEEEEEECCCCCCCCCHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCC
KAGILKAGAPAVIAPQPPEAAQTLAARATAVGAPTLWAGRDWSWERTTTGWRFAGLDLPL
CCCEEECCCCEEECCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCEEEECCCCCC
PVLAGPHQLGNAAQALAVIDALEARTGLRVEGVARARGLRAVDWPARLQRLDSGPLVERL
HHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCEECCCHHHHHHCCCCCHHHHH
APGTTLWLDGGHNPHAGRALAAALGDDASGPLDVICGMLDTKDAGGFLGALAPLVRRLRC
CCCCEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHEE
VAVPDGQTGAGLAPDALAGHARAAGIADARSCASVAEALTDLGAGTTPPPAARILICGSL
EECCCCCCCCCCCCHHHHCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHH
YLAGTVLAENGTPPR
HHHEEEEECCCCCCC
>Mature Secondary Structure
MNAPDGVLARLMGLHPKVIDLSLGRVERLLAALGHPERALPPVIHVAGTNGKGSVVATCR
CCCCHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHH
ALLEAAGYRVHVYTSPHLVRFGERIRLAGTLIADEALRRLLEEVESANAKAPITFFEVTT
HHHHHCCCEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHH
AAAFLAFSRSPADVTLLEVGLGGRLDATNVIADPAVCAVTPIDLDHQQYLGSTLTAIAGE
HHHHHHHCCCCCCEEEEEECCCCCCCCCHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCC
KAGILKAGAPAVIAPQPPEAAQTLAARATAVGAPTLWAGRDWSWERTTTGWRFAGLDLPL
CCCEEECCCCEEECCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCEEEECCCCCC
PVLAGPHQLGNAAQALAVIDALEARTGLRVEGVARARGLRAVDWPARLQRLDSGPLVERL
HHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCEECCCHHHHHHCCCCCHHHHH
APGTTLWLDGGHNPHAGRALAAALGDDASGPLDVICGMLDTKDAGGFLGALAPLVRRLRC
CCCCEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHEE
VAVPDGQTGAGLAPDALAGHARAAGIADARSCASVAEALTDLGAGTTPPPAARILICGSL
EECCCCCCCCCCCCHHHHCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHH
YLAGTVLAENGTPPR
HHHEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]