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The map label for this gene is pckG [H]

Identifier: 83594748

GI number: 83594748

Start: 3935093

End: 3936955

Strand: Reverse

Name: pckG [H]

Synonym: Rru_A3419

Alternate gene names: 83594748

Gene position: 3936955-3935093 (Counterclockwise)

Preceding gene: 83594749

Following gene: 83594747

Centisome position: 90.45

GC content: 64.84

Gene sequence:

>1863_bases
ATGCTGGAGGAAGAGATGAGCGTCAAGCCAACCCGCAACAAGAAACTTCTCGACTGGGTCGAGGACATGACGGCCCTGTG
TAAACCCGATCGGGTTTACTGGTGCGATGGATCCCAGGAAGAATACGACCGTCTTTGTCAGGCCATGGTCGATGATGGGA
CCTTCATCCGTCTCAACCCCGAGCTGCGCCCCAACAGCTTTCTGGCCCGCTCCGATCCGGCCGACGTCGCCCGGGTCGAA
GACCGCACCTTCATTTGCGCCGCCACCGCCGACGAGGCCGGCCCGACCAACAACTGGATGGCGCCCGACGAGATGCGCGC
CAAGCTGGCCGGGTTGTTCGACGGCTGCATGAAGGGCCGCACCCTTTACGTCATCCCCTTCTCGATGGGCCCGCTGGGCA
GCCATATCGCCCAGATCGGCGTCGAGATCTCCGACAGCCCCTATGTCGCGGCCAATATGCGCATCATGACGCGCATGGGC
TCGAAGGTGCTTGACGTTCTGGGCGAGGACGGCGATTTCGTGCCGGCCATGCATTCCGTCGGCAAGCCTTTGGCCCCGGG
CGAGGTCGACAAGGGCTGGCCCTGCAATCCCGAGAACCGCTACATCGTCCACTTCCCCGACACCCGGGAAATCTGGTCCT
TCGGCTCCGGGTACGGCGGCAACGCCCTGCTGGGCAAGAAGTGCTTCGCGCTGCGCATCGCCTCGGTGATGGCGCGCGAC
GAAGGCTGGCTGGCCGAACACATGCTGATCCTTGGCGTGAAAAGCCCGACGGGCGAGAAGCGCTATGTCGGCGGCGCCTT
CCCCAGCGCCTGCGGCAAGACCAACTTCGCCATGCTGATTCCGCCCGAGGCCTATAAGGGTTGGGAAGTCACCACCATCG
GCGACGATATCGCCTGGATCAAGCCGGGCGCCGACGGGCGGCTTTACGCCATCAATCCCGAGGCCGGCTATTTCGGCGTG
GCGCCGGGCACCGGGGTTCATACCAACCCCAACGCCATGATCGCCTGCGCCAAGAACTCGATCTTCACCAATGTCGCGCT
GACCGATGACGGCGACGTGTGGTGGGAAGGCATGACCGAGAAAAAGCCGGCCCATGCCATCGACTGGAAGGGCCAGGACT
GGACCCCCGATTGCGGCCGCCCCGCCGCCCATCCCAACGCCCGCTTCACCGCTCCGGCCAATCAGGCGCCCTCGATCGAC
CCCGATTGGGAGAACCCCGACGGCGTGCCGATCAGCGCCTTCATCTTCGGCGGCCGGGTGGCGACCAACTTCCCGCTGGC
CTTCCAAAGCTTTGACTGGACCCACGGCGTTTACCTCGCCGCCACCATGGGCTCGGAAGCCACCGCCGCCGCCGATGGCC
AGTCGGGCATCCGCCGCGATCCGATGGCGATGCTGCCGTTCTGCGGCTACAACATGGCCGACTACTGGCAGCATTGGCTC
GACATGGAAGACAAGGTCGGCGCCGACAAGCTGCCGCGCATCTTCCGCGTGAACTGGTTCCGCAAGGACGAGAACGGCAA
GTTCATCTGGCCGGGCTTTGGCGAGAACATGCGCGCCCTGGAATGGATCATCGGTCGTTGCGAAGGGACGGCGGGCGCGG
TGGAAAGCCCGATCGGCTTCGTGCCGCGCCGCGAGGATCTGCGGTGGGACGGCATCGTCGGCTTCGATGTCAGTCGCTTC
TATCAGATCATGGCCATCCACAAGGATGTCGCCCGCAAGGAAGCCGAGACCCAGGACGTGTTCTTCGCCAAGTTCGGTGA
TCGCATGCCCAAGGCTCTGCTCGACCAGCGGACCGCCCTGCTCAAGCGCCTGGAAGACGCTCCCGAGGTGTGGGACCTGC
CCCGCGCCGAGGCCGCCCAGTAA

Upstream 100 bases:

>100_bases
GACTAAGAAAAAGGGCGAGCCATGCCGCCCGATGCAAATCCCGCTTACGGGATATTGCTCCAATCGCCGGAGCCCGCTAG
GGTTTCGAAAAATACGACAA

Downstream 100 bases:

>100_bases
CACGGGCCCTTTCTCTCTTCCTTCATCGCCTGCCGGCCGGCTCCCCCAAGGAGCCGGCCTTTTCTTTGACCGGAAGCTGG
GCTGGGGGTGGCGCGCCCCC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MLEEEMSVKPTRNKKLLDWVEDMTALCKPDRVYWCDGSQEEYDRLCQAMVDDGTFIRLNPELRPNSFLARSDPADVARVE
DRTFICAATADEAGPTNNWMAPDEMRAKLAGLFDGCMKGRTLYVIPFSMGPLGSHIAQIGVEISDSPYVAANMRIMTRMG
SKVLDVLGEDGDFVPAMHSVGKPLAPGEVDKGWPCNPENRYIVHFPDTREIWSFGSGYGGNALLGKKCFALRIASVMARD
EGWLAEHMLILGVKSPTGEKRYVGGAFPSACGKTNFAMLIPPEAYKGWEVTTIGDDIAWIKPGADGRLYAINPEAGYFGV
APGTGVHTNPNAMIACAKNSIFTNVALTDDGDVWWEGMTEKKPAHAIDWKGQDWTPDCGRPAAHPNARFTAPANQAPSID
PDWENPDGVPISAFIFGGRVATNFPLAFQSFDWTHGVYLAATMGSEATAAADGQSGIRRDPMAMLPFCGYNMADYWQHWL
DMEDKVGADKLPRIFRVNWFRKDENGKFIWPGFGENMRALEWIIGRCEGTAGAVESPIGFVPRREDLRWDGIVGFDVSRF
YQIMAIHKDVARKEAETQDVFFAKFGDRMPKALLDQRTALLKRLEDAPEVWDLPRAEAAQ

Sequences:

>Translated_620_residues
MLEEEMSVKPTRNKKLLDWVEDMTALCKPDRVYWCDGSQEEYDRLCQAMVDDGTFIRLNPELRPNSFLARSDPADVARVE
DRTFICAATADEAGPTNNWMAPDEMRAKLAGLFDGCMKGRTLYVIPFSMGPLGSHIAQIGVEISDSPYVAANMRIMTRMG
SKVLDVLGEDGDFVPAMHSVGKPLAPGEVDKGWPCNPENRYIVHFPDTREIWSFGSGYGGNALLGKKCFALRIASVMARD
EGWLAEHMLILGVKSPTGEKRYVGGAFPSACGKTNFAMLIPPEAYKGWEVTTIGDDIAWIKPGADGRLYAINPEAGYFGV
APGTGVHTNPNAMIACAKNSIFTNVALTDDGDVWWEGMTEKKPAHAIDWKGQDWTPDCGRPAAHPNARFTAPANQAPSID
PDWENPDGVPISAFIFGGRVATNFPLAFQSFDWTHGVYLAATMGSEATAAADGQSGIRRDPMAMLPFCGYNMADYWQHWL
DMEDKVGADKLPRIFRVNWFRKDENGKFIWPGFGENMRALEWIIGRCEGTAGAVESPIGFVPRREDLRWDGIVGFDVSRF
YQIMAIHKDVARKEAETQDVFFAKFGDRMPKALLDQRTALLKRLEDAPEVWDLPRAEAAQ
>Mature_620_residues
MLEEEMSVKPTRNKKLLDWVEDMTALCKPDRVYWCDGSQEEYDRLCQAMVDDGTFIRLNPELRPNSFLARSDPADVARVE
DRTFICAATADEAGPTNNWMAPDEMRAKLAGLFDGCMKGRTLYVIPFSMGPLGSHIAQIGVEISDSPYVAANMRIMTRMG
SKVLDVLGEDGDFVPAMHSVGKPLAPGEVDKGWPCNPENRYIVHFPDTREIWSFGSGYGGNALLGKKCFALRIASVMARD
EGWLAEHMLILGVKSPTGEKRYVGGAFPSACGKTNFAMLIPPEAYKGWEVTTIGDDIAWIKPGADGRLYAINPEAGYFGV
APGTGVHTNPNAMIACAKNSIFTNVALTDDGDVWWEGMTEKKPAHAIDWKGQDWTPDCGRPAAHPNARFTAPANQAPSID
PDWENPDGVPISAFIFGGRVATNFPLAFQSFDWTHGVYLAATMGSEATAAADGQSGIRRDPMAMLPFCGYNMADYWQHWL
DMEDKVGADKLPRIFRVNWFRKDENGKFIWPGFGENMRALEWIIGRCEGTAGAVESPIGFVPRREDLRWDGIVGFDVSRF
YQIMAIHKDVARKEAETQDVFFAKFGDRMPKALLDQRTALLKRLEDAPEVWDLPRAEAAQ

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI66346721, Length=604, Percent_Identity=51.158940397351, Blast_Score=599, Evalue=1e-171,
Organism=Homo sapiens, GI187281517, Length=600, Percent_Identity=49.8333333333333, Blast_Score=590, Evalue=1e-168,
Organism=Homo sapiens, GI66346723, Length=375, Percent_Identity=54.4, Blast_Score=394, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17555492, Length=617, Percent_Identity=47.6499189627229, Blast_Score=559, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI71989645, Length=612, Percent_Identity=48.0392156862745, Blast_Score=557, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI71989658, Length=612, Percent_Identity=48.0392156862745, Blast_Score=556, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI71989653, Length=599, Percent_Identity=48.7479131886477, Blast_Score=552, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI32564411, Length=557, Percent_Identity=49.7307001795332, Blast_Score=536, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI32564407, Length=525, Percent_Identity=50.0952380952381, Blast_Score=513, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI71989649, Length=485, Percent_Identity=51.1340206185567, Blast_Score=483, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI32564409, Length=371, Percent_Identity=46.3611859838275, Blast_Score=327, Evalue=8e-90,
Organism=Caenorhabditis elegans, GI17540956, Length=483, Percent_Identity=25.0517598343685, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24655085, Length=602, Percent_Identity=51.1627906976744, Blast_Score=595, Evalue=1e-170,
Organism=Drosophila melanogaster, GI24655082, Length=603, Percent_Identity=49.4195688225539, Blast_Score=580, Evalue=1e-165,
Organism=Drosophila melanogaster, GI161077226, Length=572, Percent_Identity=50, Blast_Score=553, Evalue=1e-157,
Organism=Drosophila melanogaster, GI24655088, Length=507, Percent_Identity=52.6627218934911, Blast_Score=520, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 68751; Mature: 68751

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEEEMSVKPTRNKKLLDWVEDMTALCKPDRVYWCDGSQEEYDRLCQAMVDDGTFIRLNP
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECC
ELRPNSFLARSDPADVARVEDRTFICAATADEAGPTNNWMAPDEMRAKLAGLFDGCMKGR
CCCCCCEECCCCCCHHEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
TLYVIPFSMGPLGSHIAQIGVEISDSPYVAANMRIMTRMGSKVLDVLGEDGDFVPAMHSV
EEEEEEECCCCHHHHHHHHCCEECCCCEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHHC
GKPLAPGEVDKGWPCNPENRYIVHFPDTREIWSFGSGYGGNALLGKKCFALRIASVMARD
CCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCC
EGWLAEHMLILGVKSPTGEKRYVGGAFPSACGKTNFAMLIPPEAYKGWEVTTIGDDIAWI
CCHHEEEEEEEEECCCCCCCCEECCCCCHHCCCCCEEEEECCCCCCCEEEEEECCCEEEE
KPGADGRLYAINPEAGYFGVAPGTGVHTNPNAMIACAKNSIFTNVALTDDGDVWWEGMTE
CCCCCCEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCEEECCCCC
KKPAHAIDWKGQDWTPDCGRPAAHPNARFTAPANQAPSIDPDWENPDGVPISAFIFGGRV
CCCCCEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEECCEE
ATNFPLAFQSFDWTHGVYLAATMGSEATAAADGQSGIRRDPMAMLPFCGYNMADYWQHWL
ECCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHC
DMEDKVGADKLPRIFRVNWFRKDENGKFIWPGFGENMRALEWIIGRCEGTAGAVESPIGF
CCCCCCCCCCCCCEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
VPRREDLRWDGIVGFDVSRFYQIMAIHKDVARKEAETQDVFFAKFGDRMPKALLDQRTAL
CCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHH
LKRLEDAPEVWDLPRAEAAQ
HHHHHCCCHHHCCCCCCCCC
>Mature Secondary Structure
MLEEEMSVKPTRNKKLLDWVEDMTALCKPDRVYWCDGSQEEYDRLCQAMVDDGTFIRLNP
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECC
ELRPNSFLARSDPADVARVEDRTFICAATADEAGPTNNWMAPDEMRAKLAGLFDGCMKGR
CCCCCCEECCCCCCHHEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
TLYVIPFSMGPLGSHIAQIGVEISDSPYVAANMRIMTRMGSKVLDVLGEDGDFVPAMHSV
EEEEEEECCCCHHHHHHHHCCEECCCCEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHHC
GKPLAPGEVDKGWPCNPENRYIVHFPDTREIWSFGSGYGGNALLGKKCFALRIASVMARD
CCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCC
EGWLAEHMLILGVKSPTGEKRYVGGAFPSACGKTNFAMLIPPEAYKGWEVTTIGDDIAWI
CCHHEEEEEEEEECCCCCCCCEECCCCCHHCCCCCEEEEECCCCCCCEEEEEECCCEEEE
KPGADGRLYAINPEAGYFGVAPGTGVHTNPNAMIACAKNSIFTNVALTDDGDVWWEGMTE
CCCCCCEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCEEECCCCC
KKPAHAIDWKGQDWTPDCGRPAAHPNARFTAPANQAPSIDPDWENPDGVPISAFIFGGRV
CCCCCEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEECCEE
ATNFPLAFQSFDWTHGVYLAATMGSEATAAADGQSGIRRDPMAMLPFCGYNMADYWQHWL
ECCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHC
DMEDKVGADKLPRIFRVNWFRKDENGKFIWPGFGENMRALEWIIGRCEGTAGAVESPIGF
CCCCCCCCCCCCCEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
VPRREDLRWDGIVGFDVSRFYQIMAIHKDVARKEAETQDVFFAKFGDRMPKALLDQRTAL
CCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHH
LKRLEDAPEVWDLPRAEAAQ
HHHHHCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA