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The map label for this gene is vraB [H]

Identifier: 83594716

GI number: 83594716

Start: 3903858

End: 3905018

Strand: Direct

Name: vraB [H]

Synonym: Rru_A3387

Alternate gene names: 83594716

Gene position: 3903858-3905018 (Clockwise)

Preceding gene: 83594715

Following gene: 83594717

Centisome position: 89.69

GC content: 72.61

Gene sequence:

>1161_bases
ATGACCGATGCGGTGATCATCGCCGCCCGCCGAACCGCCGTGGGGCGGACGGCGGGCCTGTTCCGGGCGCGCAGCGCCCC
CCAATTGGCCGCGCCGGTCATCAACGCCCTGCTCGCCGATTGCCAGCTTGATGGCGCCGATATCGACGAGGTGATCTTGG
GCAATGTTCTGCAGGGGGGCAATGTCGCGCGCTCCTGCGCCCTCGAGGCCGGTCTGCCGCCGGCGGTGCCGGCGCTCAGC
GTCGATCGCCAATGCGGCTCGGGGCTTGAAGCGATCTTGCAAGCGGCCTGGAAGATCCGCGCCGGGGCGGCCAGGGTTAT
CCTGGCCGGGGGGGTGGAAAGCACCAGTTGCGCGCCCTGGCGGGTGGAACGGCCGTCCTCGCCCACCGATTTGCCGCGCT
TCGTCGCCCAGGCGCCGTTTTCGGGCGGCGGGCACCGCGATCCGTCGATGATCGAAGGCGCCGATGCGGTGGCCGAGACC
TGCGGCATCGGCCGCGCCGCCCAGGACGCCTATGCCGCCGGCAGCCATGCCCGCGCCCTGGCCGCCCAGGCGGCCGGCCG
CTTCGCCGGGGAATTGGTGTCGGTCTATGGCCCACCCGAGGCCGACGAGGGCCCGCGCCCCGGGCTGACCGCCGCCCGGC
TTGGCCGCTTGAAGCCGCTGCGGGCGGGGGGCACCGTCACCGTGGGCAATTCCTGCGCCACCAATGACTGCGCCGCCCTG
CTGATGGTGGTCGAGGCCGGCTTCGCCCGCCGCTTGGGGGTAACCCACGCCTTGCGCATCGTTGATGGCGCGGCGGGCGG
CGGCGATCCGGCCTTTCCCGGGCTTGGCGCGGTGCCGGCGGCCCGCCATCTGGCCCGGCGGGCGGGGGGGCTGGTCTTCG
CCACGCTTGATCAGATCGAATTCAACGAGGCCTTCGCCGCCCAGACCCTGGCCTGTCTGGCCCAGCTTTCGATCCCCGAG
GCCCTGGTCTGCCCGGGGGGTGGGGCTTTGGCCCTTGGTCATCCCTATGGCGCCTCCGGGGCGATCTTGATGACCCGGCT
GTTCCATGACCTGACGGGCGCCCTGCCGACGGGGGAGCAGCGCCGGGGCATGGCCTTGCTGTCGATCGCCGGTGGCCTGG
GGTTGGCGGCCACGGTCGAAACCGTGACCTTCCCGCGATGA

Upstream 100 bases:

>100_bases
GCCGTTGTCGCCGGTCTGGTGGCGGTGACCGTGCGTCGGGGCTATCCGGCGCTGTCGTCGCGCTGACCCCCCTCCTCCCT
TGGCTCTCCAGGATCCCTCC

Downstream 100 bases:

>100_bases
AGGACGATCGCCCCCCGCCGACGCCCCAGCGCCCGCCGTCACCTTTGGTGTGGTGGGCGGCCGCGCGCCCACGCACCCTG
GGGCTGTCGCTTGCGCCGGT

Product: acetyl-CoA C-acetyltransferase

Products: Acetoacetyl-CoA; Coenzyme A [C]

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTDAVIIAARRTAVGRTAGLFRARSAPQLAAPVINALLADCQLDGADIDEVILGNVLQGGNVARSCALEAGLPPAVPALS
VDRQCGSGLEAILQAAWKIRAGAARVILAGGVESTSCAPWRVERPSSPTDLPRFVAQAPFSGGGHRDPSMIEGADAVAET
CGIGRAAQDAYAAGSHARALAAQAAGRFAGELVSVYGPPEADEGPRPGLTAARLGRLKPLRAGGTVTVGNSCATNDCAAL
LMVVEAGFARRLGVTHALRIVDGAAGGGDPAFPGLGAVPAARHLARRAGGLVFATLDQIEFNEAFAAQTLACLAQLSIPE
ALVCPGGGALALGHPYGASGAILMTRLFHDLTGALPTGEQRRGMALLSIAGGLGLAATVETVTFPR

Sequences:

>Translated_386_residues
MTDAVIIAARRTAVGRTAGLFRARSAPQLAAPVINALLADCQLDGADIDEVILGNVLQGGNVARSCALEAGLPPAVPALS
VDRQCGSGLEAILQAAWKIRAGAARVILAGGVESTSCAPWRVERPSSPTDLPRFVAQAPFSGGGHRDPSMIEGADAVAET
CGIGRAAQDAYAAGSHARALAAQAAGRFAGELVSVYGPPEADEGPRPGLTAARLGRLKPLRAGGTVTVGNSCATNDCAAL
LMVVEAGFARRLGVTHALRIVDGAAGGGDPAFPGLGAVPAARHLARRAGGLVFATLDQIEFNEAFAAQTLACLAQLSIPE
ALVCPGGGALALGHPYGASGAILMTRLFHDLTGALPTGEQRRGMALLSIAGGLGLAATVETVTFPR
>Mature_385_residues
TDAVIIAARRTAVGRTAGLFRARSAPQLAAPVINALLADCQLDGADIDEVILGNVLQGGNVARSCALEAGLPPAVPALSV
DRQCGSGLEAILQAAWKIRAGAARVILAGGVESTSCAPWRVERPSSPTDLPRFVAQAPFSGGGHRDPSMIEGADAVAETC
GIGRAAQDAYAAGSHARALAAQAAGRFAGELVSVYGPPEADEGPRPGLTAARLGRLKPLRAGGTVTVGNSCATNDCAALL
MVVEAGFARRLGVTHALRIVDGAAGGGDPAFPGLGAVPAARHLARRAGGLVFATLDQIEFNEAFAAQTLACLAQLSIPEA
LVCPGGGALALGHPYGASGAILMTRLFHDLTGALPTGEQRRGMALLSIAGGLGLAATVETVTFPR

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=33.6683417085427, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI167614485, Length=396, Percent_Identity=32.3232323232323, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI4501853, Length=400, Percent_Identity=33.5, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI4557237, Length=390, Percent_Identity=33.5897435897436, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI194353979, Length=382, Percent_Identity=28.0104712041885, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI4504327, Length=388, Percent_Identity=27.319587628866, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI87082165, Length=395, Percent_Identity=37.9746835443038, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1787663, Length=408, Percent_Identity=38.9705882352941, Blast_Score=194, Evalue=6e-51,
Organism=Escherichia coli, GI1788554, Length=400, Percent_Identity=38.5, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI48994986, Length=401, Percent_Identity=36.6583541147132, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1788683, Length=418, Percent_Identity=29.1866028708134, Blast_Score=122, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI133906874, Length=393, Percent_Identity=33.3333333333333, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25147385, Length=362, Percent_Identity=30.6629834254144, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17535921, Length=378, Percent_Identity=30.6878306878307, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17551802, Length=436, Percent_Identity=29.1284403669725, Blast_Score=111, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6322031, Length=399, Percent_Identity=31.5789473684211, Blast_Score=155, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6325229, Length=372, Percent_Identity=31.4516129032258, Blast_Score=126, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24655093, Length=397, Percent_Identity=35.5163727959698, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI17648125, Length=394, Percent_Identity=32.2335025380711, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24640423, Length=366, Percent_Identity=31.1475409836066, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17137578, Length=416, Percent_Identity=28.6057692307692, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.9 [C]

Molecular weight: Translated: 38905; Mature: 38774

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAVIIAARRTAVGRTAGLFRARSAPQLAAPVINALLADCQLDGADIDEVILGNVLQGG
CCCCEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
NVARSCALEAGLPPAVPALSVDRQCGSGLEAILQAAWKIRAGAARVILAGGVESTSCAPW
CHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCE
RVERPSSPTDLPRFVAQAPFSGGGHRDPSMIEGADAVAETCGIGRAAQDAYAAGSHARAL
EECCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHH
AAQAAGRFAGELVSVYGPPEADEGPRPGLTAARLGRLKPLRAGGTVTVGNSCATNDCAAL
HHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHCCCCEECCCCEEEECCCCCCHHHHHH
LMVVEAGFARRLGVTHALRIVDGAAGGGDPAFPGLGAVPAARHLARRAGGLVFATLDQIE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEECHHH
FNEAFAAQTLACLAQLSIPEALVCPGGGALALGHPYGASGAILMTRLFHDLTGALPTGEQ
HHHHHHHHHHHHHHHHCCCCEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCH
RRGMALLSIAGGLGLAATVETVTFPR
HCCEEEEEECCCCCHHEEEHHCCCCC
>Mature Secondary Structure 
TDAVIIAARRTAVGRTAGLFRARSAPQLAAPVINALLADCQLDGADIDEVILGNVLQGG
CCCEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
NVARSCALEAGLPPAVPALSVDRQCGSGLEAILQAAWKIRAGAARVILAGGVESTSCAPW
CHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCE
RVERPSSPTDLPRFVAQAPFSGGGHRDPSMIEGADAVAETCGIGRAAQDAYAAGSHARAL
EECCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHH
AAQAAGRFAGELVSVYGPPEADEGPRPGLTAARLGRLKPLRAGGTVTVGNSCATNDCAAL
HHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHCCCCEECCCCEEEECCCCCCHHHHHH
LMVVEAGFARRLGVTHALRIVDGAAGGGDPAFPGLGAVPAARHLARRAGGLVFATLDQIE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEECHHH
FNEAFAAQTLACLAQLSIPEALVCPGGGALALGHPYGASGAILMTRLFHDLTGALPTGEQ
HHHHHHHHHHHHHHHHCCCCEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCH
RRGMALLSIAGGLGLAATVETVTFPR
HCCEEEEEECCCCCHHEEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acetyl-CoA [C]

Specific reaction: (2) Acetyl-CoA <==> Acetoacetyl-CoA + Coenzyme A [C]

General reaction: Acyl group transfer; Condensation; Thiolytic cleavage [C]

Inhibitor: Acetoacetyl-CoA; Borohydride; Iodoacetamide; N-Ethyl maleimide; p-Chloromercuribenzoate; Sulfhydryl reagents [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]