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The map label for this gene is argF [H]

Identifier: 83594606

GI number: 83594606

Start: 3773691

End: 3774617

Strand: Reverse

Name: argF [H]

Synonym: Rru_A3276

Alternate gene names: 83594606

Gene position: 3774617-3773691 (Counterclockwise)

Preceding gene: 83594607

Following gene: 83594605

Centisome position: 86.72

GC content: 66.45

Gene sequence:

>927_bases
ATGACCGTGATGAAAACCCCCCGGCATTTCCTGGAGTTGTCGGATTTCGACGCGCCGGCCCTGCGCGCCATGCTCGATCG
CGGGCTGGCCTTCAAGGCCGAGCGGCGGGCGGGTGTGGTCTCGGCGCCCTTGGCCGGCAAGACCCTGGCGCTGATCTTCG
AAAAGCCCTCGACCCGCACCCGGGTGTCCTTCGAGGTCGGCATGCGCCAGCTTGGCGGATCGTCGATCACGCTGAGCCCG
ACGGAAACCCAATTGGGGCGCGGCGAAACCATCGCCGATACCGCCCGCGTGCTGTCGCGCTATGTCGATGGCATCATGCT
GCGCACCAACGATCCGGCCAAGTTGCGCGATCTGAGCCAGAACGCCAGCGTGCCGGTGATCAACGGCCTGACCGATGCCG
CCCATCCCTGTCAGATCATGGCCGATCTGATGACCTTCGAAGAGCATCGCGGGTCGATCACGGGCAAGGTGGTGACTTGG
GTCGGCGATGGCAACAACGTGGCCTGTTCGTGGATCAAGGCCAGCGCCCTGTTCGGCTTCACCATGCGTCTGGCCTGCCC
GGCCGAGCTTGAACCGCCGCAAGGCGTGCTCGATTGGGCGCGGGCCAATGGCGGCGCGGTCGAGGTACTGCGCGATCCGC
AAGAGGCGGTGGCCGGAGCCGATTGCGTGGTGACCGATACCTGGGTTTCGATGGGCTGCGACGGCGCCGCCGCCCGCCAT
GCCCTGTTGCGTCCCTATCAGGTGACGGCGCCGCTGATGGCTGCCGCCGCCGCCGACGCCTTGTTCATGCATTGCCTGCC
CGCCCATCGCGGCGAGGAGGTGACCGAAGACGTCATCGACGGCCCGCAGTCGGTGGTCTGGGATGAAGCGGAGAACCGTC
TGCACGCGCAGAAGGGGATCCTTGCTTGGTGTATGGCCGGCTCGTAA

Upstream 100 bases:

>100_bases
GGTTGTTGCCGCCGATGATCATCGGCGAGGCCGAGGTCACCGCCGCCGAAGAGATGATCGACCGCGCCTGCGTCCATCTG
GAACGGGAGGCGGCGTGATC

Downstream 100 bases:

>100_bases
CGCCGGCGTCCGGGGAGGGAGGCCGTCACCACGGCCGTCCCTTCGCCGCAGAAGGGGGATAACGGCGTGAGCGGACCTTC
CGACAACCTGATTTTACCCT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTVMKTPRHFLELSDFDAPALRAMLDRGLAFKAERRAGVVSAPLAGKTLALIFEKPSTRTRVSFEVGMRQLGGSSITLSP
TETQLGRGETIADTARVLSRYVDGIMLRTNDPAKLRDLSQNASVPVINGLTDAAHPCQIMADLMTFEEHRGSITGKVVTW
VGDGNNVACSWIKASALFGFTMRLACPAELEPPQGVLDWARANGGAVEVLRDPQEAVAGADCVVTDTWVSMGCDGAAARH
ALLRPYQVTAPLMAAAAADALFMHCLPAHRGEEVTEDVIDGPQSVVWDEAENRLHAQKGILAWCMAGS

Sequences:

>Translated_308_residues
MTVMKTPRHFLELSDFDAPALRAMLDRGLAFKAERRAGVVSAPLAGKTLALIFEKPSTRTRVSFEVGMRQLGGSSITLSP
TETQLGRGETIADTARVLSRYVDGIMLRTNDPAKLRDLSQNASVPVINGLTDAAHPCQIMADLMTFEEHRGSITGKVVTW
VGDGNNVACSWIKASALFGFTMRLACPAELEPPQGVLDWARANGGAVEVLRDPQEAVAGADCVVTDTWVSMGCDGAAARH
ALLRPYQVTAPLMAAAAADALFMHCLPAHRGEEVTEDVIDGPQSVVWDEAENRLHAQKGILAWCMAGS
>Mature_307_residues
TVMKTPRHFLELSDFDAPALRAMLDRGLAFKAERRAGVVSAPLAGKTLALIFEKPSTRTRVSFEVGMRQLGGSSITLSPT
ETQLGRGETIADTARVLSRYVDGIMLRTNDPAKLRDLSQNASVPVINGLTDAAHPCQIMADLMTFEEHRGSITGKVVTWV
GDGNNVACSWIKASALFGFTMRLACPAELEPPQGVLDWARANGGAVEVLRDPQEAVAGADCVVTDTWVSMGCDGAAARHA
LLRPYQVTAPLMAAAAADALFMHCLPAHRGEEVTEDVIDGPQSVVWDEAENRLHAQKGILAWCMAGS

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=293, Percent_Identity=39.9317406143345, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI18105007, Length=309, Percent_Identity=24.5954692556634, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1790703, Length=322, Percent_Identity=39.7515527950311, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI1786469, Length=322, Percent_Identity=39.4409937888199, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI2367364, Length=307, Percent_Identity=30.9446254071661, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI48994908, Length=331, Percent_Identity=27.190332326284, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI193204318, Length=282, Percent_Identity=27.6595744680851, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6322373, Length=318, Percent_Identity=34.5911949685535, Blast_Score=182, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6322331, Length=306, Percent_Identity=28.4313725490196, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24642586, Length=304, Percent_Identity=24.3421052631579, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 33025; Mature: 32894

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVMKTPRHFLELSDFDAPALRAMLDRGLAFKAERRAGVVSAPLAGKTLALIFEKPSTRT
CCCCCCCHHHHHCCCCCHHHHHHHHHCCCEEEHHHCCCEEECCCCCCEEEEEEECCCCCE
RVSFEVGMRQLGGSSITLSPTETQLGRGETIADTARVLSRYVDGIMLRTNDPAKLRDLSQ
EEEHHHHHHHHCCCEEEECCCHHHCCCCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHCC
NASVPVINGLTDAAHPCQIMADLMTFEEHRGSITGKVVTWVGDGNNVACSWIKASALFGF
CCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEEEHHHHHHHH
TMRLACPAELEPPQGVLDWARANGGAVEVLRDPQEAVAGADCVVTDTWVSMGCDGAAARH
HEEEECCCCCCCCCCHHHHHHCCCCEEEEEECHHHHHCCCCEEEEHHHHHCCCCCHHHHH
ALLRPYQVTAPLMAAAAADALFMHCLPAHRGEEVTEDVIDGPQSVVWDEAENRLHAQKGI
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCE
LAWCMAGS
EEEEECCC
>Mature Secondary Structure 
TVMKTPRHFLELSDFDAPALRAMLDRGLAFKAERRAGVVSAPLAGKTLALIFEKPSTRT
CCCCCCHHHHHCCCCCHHHHHHHHHCCCEEEHHHCCCEEECCCCCCEEEEEEECCCCCE
RVSFEVGMRQLGGSSITLSPTETQLGRGETIADTARVLSRYVDGIMLRTNDPAKLRDLSQ
EEEHHHHHHHHCCCEEEECCCHHHCCCCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHCC
NASVPVINGLTDAAHPCQIMADLMTFEEHRGSITGKVVTWVGDGNNVACSWIKASALFGF
CCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEEEHHHHHHHH
TMRLACPAELEPPQGVLDWARANGGAVEVLRDPQEAVAGADCVVTDTWVSMGCDGAAARH
HEEEECCCCCCCCCCHHHHHHCCCCEEEEEECHHHHHCCCCEEEEHHHHHCCCCCHHHHH
ALLRPYQVTAPLMAAAAADALFMHCLPAHRGEEVTEDVIDGPQSVVWDEAENRLHAQKGI
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCE
LAWCMAGS
EEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]