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The map label for this gene is fabI2 [H]

Identifier: 83594556

GI number: 83594556

Start: 3720086

End: 3720859

Strand: Reverse

Name: fabI2 [H]

Synonym: Rru_A3226

Alternate gene names: 83594556

Gene position: 3720859-3720086 (Counterclockwise)

Preceding gene: 83594557

Following gene: 83594555

Centisome position: 85.48

GC content: 62.4

Gene sequence:

>774_bases
ATGGACACTCCATTCAGCCTGCGGGGCAAGAAGGGGCTGGTCGTGGGCATCGCCAACGACCAGAGCATCGCCTTTGGCTG
TGCGCGCTATTTCAAGAAGATGGGCGCCGATATCGCCGTCACCTATCTGAACGAAAAGGCCAAGCCCTATGTCGAGCCTT
TGGCTGGGGATCTGGACGCCTTGCTGCTTGGTCCCTGCGACGTGCGCGAATCCGGCCAGCTCAAGGGCGTTTTCGATCGC
ATCGCCGAGGAGTGGGGCCAACTGGATTTCTTCCTGCATTCGATCGCCTTCGCCCCCAAGGAAGACCTGCACGCCCCGGT
CATCGATTGTTCGCGCGAAGGCTTCTCGCTGGCGATGGATGTCTCGTGCCATTCTTTCGTGCGCATGGCCAAATACGCCG
AGCCGCTGATGAAAAACGGCGGCTCGATGCTGACCGTCACCTTCTATGGCGGGTTGAAGGTGGTCGAGCATTACAACATG
ATGGGACCGGTCAAGGCGGCGCTTGAGGGGGTGACGCGCTATCTGGCCGCCGATCTCGGTCCCAAGGGGATCCGCGTCAA
CGCCATTTCGCCGGGGCCTTTGCGCACGCGGGCGGCTTCGGGCCTCGATCGCTTCGACGAACTGCTGGAAGCCGCGGCCA
AGCGCGCCCCCCAGCACCAGTTGGTGACCATCGATGATTGCGGGGCGCTGGCCGCCTTCCTGGTTGGCGATGGCGCCAAG
GCGATGACCGGGGCGACCTATTTCGTCGACGGCGGCTATAACATCATGGGCTGA

Upstream 100 bases:

>100_bases
ACACCCGAACCCAGTCCTTATATTGCGTTGCAGCAATCCCTTTTCCGACGGTGGGCGGCAAGGGGGCTTGCCGTTTTCCA
CTTTGCAAGGGATCGCATCT

Downstream 100 bases:

>100_bases
GGCCAAGCCGGTCGTGGCGGGGAGGCGGCGCGCGCCGTCTCCCTTGCCGAGGCCCTTGCCAAACGCCCGCGTCTCGCTTA
TATCCCTTGGCCGACGCGGG

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 2 [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MDTPFSLRGKKGLVVGIANDQSIAFGCARYFKKMGADIAVTYLNEKAKPYVEPLAGDLDALLLGPCDVRESGQLKGVFDR
IAEEWGQLDFFLHSIAFAPKEDLHAPVIDCSREGFSLAMDVSCHSFVRMAKYAEPLMKNGGSMLTVTFYGGLKVVEHYNM
MGPVKAALEGVTRYLAADLGPKGIRVNAISPGPLRTRAASGLDRFDELLEAAAKRAPQHQLVTIDDCGALAAFLVGDGAK
AMTGATYFVDGGYNIMG

Sequences:

>Translated_257_residues
MDTPFSLRGKKGLVVGIANDQSIAFGCARYFKKMGADIAVTYLNEKAKPYVEPLAGDLDALLLGPCDVRESGQLKGVFDR
IAEEWGQLDFFLHSIAFAPKEDLHAPVIDCSREGFSLAMDVSCHSFVRMAKYAEPLMKNGGSMLTVTFYGGLKVVEHYNM
MGPVKAALEGVTRYLAADLGPKGIRVNAISPGPLRTRAASGLDRFDELLEAAAKRAPQHQLVTIDDCGALAAFLVGDGAK
AMTGATYFVDGGYNIMG
>Mature_257_residues
MDTPFSLRGKKGLVVGIANDQSIAFGCARYFKKMGADIAVTYLNEKAKPYVEPLAGDLDALLLGPCDVRESGQLKGVFDR
IAEEWGQLDFFLHSIAFAPKEDLHAPVIDCSREGFSLAMDVSCHSFVRMAKYAEPLMKNGGSMLTVTFYGGLKVVEHYNM
MGPVKAALEGVTRYLAADLGPKGIRVNAISPGPLRTRAASGLDRFDELLEAAAKRAPQHQLVTIDDCGALAAFLVGDGAK
AMTGATYFVDGGYNIMG

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=238, Percent_Identity=24.7899159663866, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI66933014, Length=234, Percent_Identity=26.0683760683761, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI10190704, Length=259, Percent_Identity=25.0965250965251, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787545, Length=250, Percent_Identity=41.2, Blast_Score=200, Evalue=8e-53,
Organism=Escherichia coli, GI87082100, Length=258, Percent_Identity=26.3565891472868, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789208, Length=259, Percent_Identity=25.8687258687259, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1789378, Length=199, Percent_Identity=26.1306532663317, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI2367365, Length=254, Percent_Identity=26.7716535433071, Blast_Score=60, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI71994600, Length=248, Percent_Identity=26.6129032258064, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324126, Length=259, Percent_Identity=26.6409266409266, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355319, Length=252, Percent_Identity=28.1746031746032, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24644337, Length=199, Percent_Identity=30.1507537688442, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357041, Length=264, Percent_Identity=25.7575757575758, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI23397609, Length=181, Percent_Identity=24.8618784530387, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27606; Mature: 27606

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTPFSLRGKKGLVVGIANDQSIAFGCARYFKKMGADIAVTYLNEKAKPYVEPLAGDLDA
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCE
LLLGPCDVRESGQLKGVFDRIAEEWGQLDFFLHSIAFAPKEDLHAPVIDCSREGFSLAMD
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHCCCCCCEEEEE
VSCHSFVRMAKYAEPLMKNGGSMLTVTFYGGLKVVEHYNMMGPVKAALEGVTRYLAADLG
CHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHCCC
PKGIRVNAISPGPLRTRAASGLDRFDELLEAAAKRAPQHQLVTIDDCGALAAFLVGDGAK
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHCCCCH
AMTGATYFVDGGYNIMG
HHCCEEEEEECCEEECC
>Mature Secondary Structure
MDTPFSLRGKKGLVVGIANDQSIAFGCARYFKKMGADIAVTYLNEKAKPYVEPLAGDLDA
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCE
LLLGPCDVRESGQLKGVFDRIAEEWGQLDFFLHSIAFAPKEDLHAPVIDCSREGFSLAMD
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHCCCCCCEEEEE
VSCHSFVRMAKYAEPLMKNGGSMLTVTFYGGLKVVEHYNMMGPVKAALEGVTRYLAADLG
CHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHCCC
PKGIRVNAISPGPLRTRAASGLDRFDELLEAAAKRAPQHQLVTIDDCGALAAFLVGDGAK
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHCCCCH
AMTGATYFVDGGYNIMG
HHCCEEEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]