Click here to switch to the map view.

The map label for this gene is pleC [H]

Identifier: 83594543

GI number: 83594543

Start: 3703369

End: 3705705

Strand: Direct

Name: pleC [H]

Synonym: Rru_A3213

Alternate gene names: 83594543

Gene position: 3703369-3705705 (Clockwise)

Preceding gene: 83594540

Following gene: 83594545

Centisome position: 85.08

GC content: 67.35

Gene sequence:

>2337_bases
GTGTCGGCGATAGACGGACCAGGATTGGACTCGCGACTAATAGATATATTCGACCACCTGGACGAGGGGGTGGCCCTCTA
TGGTCCCGATCATCGTCTGATCCGTTTCAACCGGCCTTTCGCCAGCCTTGTCGAAACCGCCGGCGGGCGTCCCGCCCCAG
GCCAAAGGATGGTCGAAATCCTGCGCGATTTGGTCGCGCCGGACACCGGGGAGGAGAATCGGGCCGACTCCGGCCCCGAC
GACGACGGGCGAGGGACCGAGGACGACGCCGCGGAGCGCCTGGAAGGAGCCCTGCTGGGAACGCCCACCGAGATCCGCCT
GGGCCGGGATCGATGGGGTCTGGTCCATGGTCACCGCCTGAAGGACGGCGCCCTGTTTTGCGTTCTCCGCGATATCACCG
CGTCAAAAGCCGGGGCCGCACGGATCGCCGAGAGCAACCGCCGCATCCGCGAGGCCGAAAGCCGCCTGGAACGCGCCCTC
GAGTCGATCGACCAGGGGTTCTATCTGTGCGACGCCAGCGACACGGTGGTGCTGCGCAACGCCAAGCTCGCCCATCTGCT
GCCGGGCTTGCCCCCCCTCGAGCCGGGCACGACCTATGGCGCGATGATGGCCGCCGCCTGTGACAACGGCGCCATCCCCG
CCGCCACGCGCCACCGCACCCGCTGGCTGAGCCTGTGCATGGCCCGCCACCATCTGCCGCGCGGCGCCTGGGACTTCGAG
AACGGCCGGGGCCGCTGGGTTCGGGTTTCCGAATCCCTTACCCCCGAGGGCGGACGGGTCGGCCTGCTGACCGACATCAC
CGAGACCCGCAAGGCCTCCGAACAGGTGCGCCAGCGCGAACAGCGCCTTGACGGCATCATGAAGACCGTCGTCGACGGCA
TCGTCGTCATCGATGAAAACGGCATTATCGAATCGTTCAATCCCGCCGCCGAACGGCTTTTCGGCTATCGGGCCGAAGAG
GTGGTGGGCGCGTCGATCAACCGGCTGATCCCGGGCGGCGAGAGCGGCCGCCACGACCGCTCCCTGCGGCGCTATCTGCA
AACCGGCCAGGCCGGCATCATCGGCCGGGGCCGCGAGGAACTGGCCCTGCGCCGCGACGGCTCGCTGCTTCCCGTCGAAC
TGGCGATCAGCGAATTGCGGCTGGACGACCGGCGCATCTTCACCGGCCTGGTGCGCGACATCAGCGCCCGCAAGGAAGCC
GATCGCCAGCTGCGCGAAAACGAGGAACGCTACGCCCTGGCCATCGCCGGCACCCATGAGGCCATCGTCGATTGGGACAT
CGACAGCGACACCATCACCTATTCCGACCGCATCACCGCCATCGTCGGCCTTGATCCCAAGGATCTGGACCGCTCGGCGA
GCTGGATCGCGCTGATCCATCCCGACCATCGCAAGGATTACTTGACGCGGATGGACGCCCTGCTGCGCGGCGAGAGTTCA
TTCTTTTCGTGCGATTTCCGTCTGGCCGGACGGGCGGGAGCCGAGGAACGCTGGCTGCGCCACCGGGCCACCGCGCTGCG
CCGCCCCGATGGCCAGATCTATCGCCTGTCGGGCGCGGTCGGCGACATCACCGACAGCCGCCGCGGCGAGGATCGCCTGC
GCGAGGCCAAGGAGGCCGCCGAATTCGCCAATCGGGCCAAAAGCGAATTCCTGACCAATATGAGCCACGAGCTGCGCACG
CCGCTCAACGCCATCATCGGCTTCTCGGAGGTCCTTCACAGCGAGATGTTCGGACCGCTGGGCGCGACGCAATACCTGGA
CTATGCCGCCAGCATCCGCGATTCGGGGCGCCATCTGCTCGACGTGATCAACGATATCCTCGATGTCAGCCGCATCGAGG
CCGGGCGGGCGACGCTGTTTCCCGAACCGCTTGATTTCGCCGAGATCGTCGCCTCGGTGCACCGGCTGGTCAACCAGAGG
GCCGAGGCCGCCGGCGTCCGCCTGAAGATCACCCTTGATCCGGCGCTGCCGCCCCTGCTTGGCGAGGCCCGCCGGCTCAA
GCAGGTTCTGATCAACCTTCTGGGCAACGCCATCAAATTCACCCCGGCCGGCGGCGAGATCGCCCTGTCGGCCAGGGGCG
ATGAGGACAAAGGCCAGATGGTGATCGAGGTCAGCGATTCGGGGATCGGCATGGCGCCCGAGGATATCCCGCTCGCCCTT
GAGCCCTTCCGTCAGATCGATGGCCAACTGGCCCGGCGCTACGAGGGGACCGGCCTGGGACTGCCGCTGTCAAAAGCCTT
CGTCGAACTGCACGGCGGGACGCTCCGAGTCGACAGCACGCCGGGCGCGGGCACCCGCATCACCGTGCGCCTGCCCGCCG
GAGCGGTGGAATCCTAA

Upstream 100 bases:

>100_bases
CTGTTAAAATCCTGTCTTGAGAAAGTCATATCAGTGAAATTCCCGAATCAGGCGAGAGAGGACTCCCTCCAACCCTTCGG
ATGAAACCCATGGGACTGCC

Downstream 100 bases:

>100_bases
AAGGTCCACCGCCCCGCGCGGATCGGACGGCCGTATCGTCAACCGCCGGCGGCGTCGAGGGCGGCGCCGATGTCGGCGGC
CAGATCCTCGGGGTCTTCCA

Product: PAS/PAC sensor Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 778; Mature: 777

Protein sequence:

>778_residues
MSAIDGPGLDSRLIDIFDHLDEGVALYGPDHRLIRFNRPFASLVETAGGRPAPGQRMVEILRDLVAPDTGEENRADSGPD
DDGRGTEDDAAERLEGALLGTPTEIRLGRDRWGLVHGHRLKDGALFCVLRDITASKAGAARIAESNRRIREAESRLERAL
ESIDQGFYLCDASDTVVLRNAKLAHLLPGLPPLEPGTTYGAMMAAACDNGAIPAATRHRTRWLSLCMARHHLPRGAWDFE
NGRGRWVRVSESLTPEGGRVGLLTDITETRKASEQVRQREQRLDGIMKTVVDGIVVIDENGIIESFNPAAERLFGYRAEE
VVGASINRLIPGGESGRHDRSLRRYLQTGQAGIIGRGREELALRRDGSLLPVELAISELRLDDRRIFTGLVRDISARKEA
DRQLRENEERYALAIAGTHEAIVDWDIDSDTITYSDRITAIVGLDPKDLDRSASWIALIHPDHRKDYLTRMDALLRGESS
FFSCDFRLAGRAGAEERWLRHRATALRRPDGQIYRLSGAVGDITDSRRGEDRLREAKEAAEFANRAKSEFLTNMSHELRT
PLNAIIGFSEVLHSEMFGPLGATQYLDYAASIRDSGRHLLDVINDILDVSRIEAGRATLFPEPLDFAEIVASVHRLVNQR
AEAAGVRLKITLDPALPPLLGEARRLKQVLINLLGNAIKFTPAGGEIALSARGDEDKGQMVIEVSDSGIGMAPEDIPLAL
EPFRQIDGQLARRYEGTGLGLPLSKAFVELHGGTLRVDSTPGAGTRITVRLPAGAVES

Sequences:

>Translated_778_residues
MSAIDGPGLDSRLIDIFDHLDEGVALYGPDHRLIRFNRPFASLVETAGGRPAPGQRMVEILRDLVAPDTGEENRADSGPD
DDGRGTEDDAAERLEGALLGTPTEIRLGRDRWGLVHGHRLKDGALFCVLRDITASKAGAARIAESNRRIREAESRLERAL
ESIDQGFYLCDASDTVVLRNAKLAHLLPGLPPLEPGTTYGAMMAAACDNGAIPAATRHRTRWLSLCMARHHLPRGAWDFE
NGRGRWVRVSESLTPEGGRVGLLTDITETRKASEQVRQREQRLDGIMKTVVDGIVVIDENGIIESFNPAAERLFGYRAEE
VVGASINRLIPGGESGRHDRSLRRYLQTGQAGIIGRGREELALRRDGSLLPVELAISELRLDDRRIFTGLVRDISARKEA
DRQLRENEERYALAIAGTHEAIVDWDIDSDTITYSDRITAIVGLDPKDLDRSASWIALIHPDHRKDYLTRMDALLRGESS
FFSCDFRLAGRAGAEERWLRHRATALRRPDGQIYRLSGAVGDITDSRRGEDRLREAKEAAEFANRAKSEFLTNMSHELRT
PLNAIIGFSEVLHSEMFGPLGATQYLDYAASIRDSGRHLLDVINDILDVSRIEAGRATLFPEPLDFAEIVASVHRLVNQR
AEAAGVRLKITLDPALPPLLGEARRLKQVLINLLGNAIKFTPAGGEIALSARGDEDKGQMVIEVSDSGIGMAPEDIPLAL
EPFRQIDGQLARRYEGTGLGLPLSKAFVELHGGTLRVDSTPGAGTRITVRLPAGAVES
>Mature_777_residues
SAIDGPGLDSRLIDIFDHLDEGVALYGPDHRLIRFNRPFASLVETAGGRPAPGQRMVEILRDLVAPDTGEENRADSGPDD
DGRGTEDDAAERLEGALLGTPTEIRLGRDRWGLVHGHRLKDGALFCVLRDITASKAGAARIAESNRRIREAESRLERALE
SIDQGFYLCDASDTVVLRNAKLAHLLPGLPPLEPGTTYGAMMAAACDNGAIPAATRHRTRWLSLCMARHHLPRGAWDFEN
GRGRWVRVSESLTPEGGRVGLLTDITETRKASEQVRQREQRLDGIMKTVVDGIVVIDENGIIESFNPAAERLFGYRAEEV
VGASINRLIPGGESGRHDRSLRRYLQTGQAGIIGRGREELALRRDGSLLPVELAISELRLDDRRIFTGLVRDISARKEAD
RQLRENEERYALAIAGTHEAIVDWDIDSDTITYSDRITAIVGLDPKDLDRSASWIALIHPDHRKDYLTRMDALLRGESSF
FSCDFRLAGRAGAEERWLRHRATALRRPDGQIYRLSGAVGDITDSRRGEDRLREAKEAAEFANRAKSEFLTNMSHELRTP
LNAIIGFSEVLHSEMFGPLGATQYLDYAASIRDSGRHLLDVINDILDVSRIEAGRATLFPEPLDFAEIVASVHRLVNQRA
EAAGVRLKITLDPALPPLLGEARRLKQVLINLLGNAIKFTPAGGEIALSARGDEDKGQMVIEVSDSGIGMAPEDIPLALE
PFRQIDGQLARRYEGTGLGLPLSKAFVELHGGTLRVDSTPGAGTRITVRLPAGAVES

Specific function: Member of the two-component regulatory system involved in the regulation of polar organelle development. PleC functions as a membrane-associated protein kinase that transfers phosphate to the response regulator pleD, leading to its activation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=233, Percent_Identity=38.1974248927039, Blast_Score=147, Evalue=3e-36,
Organism=Escherichia coli, GI145693157, Length=258, Percent_Identity=36.4341085271318, Blast_Score=145, Evalue=1e-35,
Organism=Escherichia coli, GI87081816, Length=242, Percent_Identity=39.6694214876033, Blast_Score=143, Evalue=4e-35,
Organism=Escherichia coli, GI48994928, Length=271, Percent_Identity=34.3173431734317, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI1788713, Length=289, Percent_Identity=32.8719723183391, Blast_Score=126, Evalue=6e-30,
Organism=Escherichia coli, GI1786600, Length=227, Percent_Identity=29.5154185022026, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI1786912, Length=243, Percent_Identity=28.8065843621399, Blast_Score=91, Evalue=4e-19,
Organism=Escherichia coli, GI1788549, Length=376, Percent_Identity=26.063829787234, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1790436, Length=219, Percent_Identity=29.6803652968037, Blast_Score=90, Evalue=6e-19,
Organism=Escherichia coli, GI1786783, Length=229, Percent_Identity=29.6943231441048, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1788393, Length=234, Percent_Identity=28.2051282051282, Blast_Score=88, Evalue=3e-18,
Organism=Escherichia coli, GI1790346, Length=227, Percent_Identity=28.6343612334802, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI87082128, Length=229, Percent_Identity=27.5109170305677, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI87081921, Length=239, Percent_Identity=27.6150627615063, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1790300, Length=285, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1790861, Length=277, Percent_Identity=27.7978339350181, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 85497; Mature: 85366

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIDGPGLDSRLIDIFDHLDEGVALYGPDHRLIRFNRPFASLVETAGGRPAPGQRMVEI
CCCCCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCHHHHHHHCCCCCCCHHHHHHH
LRDLVAPDTGEENRADSGPDDDGRGTEDDAAERLEGALLGTPTEIRLGRDRWGLVHGHRL
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHCCCCCCEEECCCHHCCCCCCEE
KDGALFCVLRDITASKAGAARIAESNRRIREAESRLERALESIDQGFYLCDASDTVVLRN
CCCEEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEC
AKLAHLLPGLPPLEPGTTYGAMMAAACDNGAIPAATRHRTRWLSLCMARHHLPRGAWDFE
CHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
NGRGRWVRVSESLTPEGGRVGLLTDITETRKASEQVRQREQRLDGIMKTVVDGIVVIDEN
CCCCCEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC
GIIESFNPAAERLFGYRAEEVVGASINRLIPGGESGRHDRSLRRYLQTGQAGIIGRGREE
CCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHH
LALRRDGSLLPVELAISELRLDDRRIFTGLVRDISARKEADRQLRENEERYALAIAGTHE
HHHHCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
AIVDWDIDSDTITYSDRITAIVGLDPKDLDRSASWIALIHPDHRKDYLTRMDALLRGESS
EEEEECCCCCEEEECCCEEEEEECCHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCC
FFSCDFRLAGRAGAEERWLRHRATALRRPDGQIYRLSGAVGDITDSRRGEDRLREAKEAA
EEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHH
EFANRAKSEFLTNMSHELRTPLNAIIGFSEVLHSEMFGPLGATQYLDYAASIRDSGRHLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHH
DVINDILDVSRIEAGRATLFPEPLDFAEIVASVHRLVNQRAEAAGVRLKITLDPALPPLL
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHH
GEARRLKQVLINLLGNAIKFTPAGGEIALSARGDEDKGQMVIEVSDSGIGMAPEDIPLAL
HHHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCHH
EPFRQIDGQLARRYEGTGLGLPLSKAFVELHGGTLRVDSTPGAGTRITVRLPAGAVES
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
SAIDGPGLDSRLIDIFDHLDEGVALYGPDHRLIRFNRPFASLVETAGGRPAPGQRMVEI
CCCCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCHHHHHHHCCCCCCCHHHHHHH
LRDLVAPDTGEENRADSGPDDDGRGTEDDAAERLEGALLGTPTEIRLGRDRWGLVHGHRL
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHCCCCCCEEECCCHHCCCCCCEE
KDGALFCVLRDITASKAGAARIAESNRRIREAESRLERALESIDQGFYLCDASDTVVLRN
CCCEEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEC
AKLAHLLPGLPPLEPGTTYGAMMAAACDNGAIPAATRHRTRWLSLCMARHHLPRGAWDFE
CHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
NGRGRWVRVSESLTPEGGRVGLLTDITETRKASEQVRQREQRLDGIMKTVVDGIVVIDEN
CCCCCEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC
GIIESFNPAAERLFGYRAEEVVGASINRLIPGGESGRHDRSLRRYLQTGQAGIIGRGREE
CCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHH
LALRRDGSLLPVELAISELRLDDRRIFTGLVRDISARKEADRQLRENEERYALAIAGTHE
HHHHCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
AIVDWDIDSDTITYSDRITAIVGLDPKDLDRSASWIALIHPDHRKDYLTRMDALLRGESS
EEEEECCCCCEEEECCCEEEEEECCHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCC
FFSCDFRLAGRAGAEERWLRHRATALRRPDGQIYRLSGAVGDITDSRRGEDRLREAKEAA
EEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHH
EFANRAKSEFLTNMSHELRTPLNAIIGFSEVLHSEMFGPLGATQYLDYAASIRDSGRHLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHH
DVINDILDVSRIEAGRATLFPEPLDFAEIVASVHRLVNQRAEAAGVRLKITLDPALPPLL
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHH
GEARRLKQVLINLLGNAIKFTPAGGEIALSARGDEDKGQMVIEVSDSGIGMAPEDIPLAL
HHHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCHH
EPFRQIDGQLARRYEGTGLGLPLSKAFVELHGGTLRVDSTPGAGTRITVRLPAGAVES
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8421698; 11259647 [H]