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The map label for this gene is lcfB [H]

Identifier: 83594464

GI number: 83594464

Start: 3613334

End: 3614950

Strand: Direct

Name: lcfB [H]

Synonym: Rru_A3134

Alternate gene names: 83594464

Gene position: 3613334-3614950 (Clockwise)

Preceding gene: 83594463

Following gene: 83594465

Centisome position: 83.01

GC content: 71.06

Gene sequence:

>1617_bases
GTGAGAGCGCTTTTTGGTCTGAGCGATCTTCTCGCCCGCGCCTGCGACCACACCCCGGCGGCCACCGCCCTGTCACAAGC
CGGCGCGATCAGCCGCTCAGCGCCGCCCTGCCTGACCTATGAAGGGTTGTTCAACCGGGTGTGCCGTTTGGCCTCCGGCT
TCAAAGCCTTGGGGCTGAGGCCGGGCGAGCGGGTGGCGGTGATCGCCGAAAAACGCTTTGACGCGGTGGCGGCGATGTTT
GCCGTCGCCCATGCCGGGGGCGTTTTCGTTCCGATCAACCCGGTGCTGAAAAGCCCGCAGATCGTCCATATCCTGGCCGA
TTCGGCAGCGAAAATCCTGGTCGCCCCGGCTCTGCGGCTGGCCTTGCTCGACCATACGCCGCCGCCCTCGCTCACCACCC
TCCTCCGCTTCGGCGACCCGGCGCCCGCCTCCCCCGATCCGCCCCCCGGGGGGGAGAGCCTTGATCTCGACGCGCTGGCC
GGGGCTGGCGATCCGATCGCCGCCCACCCCGTCGTCGATGACGATCCGGCCTGCTTTTTCTATACCTCGGGCTCCACCGG
CCTGCCCAAGGCGGTGGTCGTCACCCACCGCAACCTGATCGCCGGGGCGCAAAGCGTCGCCAGCCGCCTGGACAACCGCC
CCGATGACCGCCTGCTCGCCGCCCTGCCCCTGGGCTTTGACGCCGGCTTCAGTCAACTGACCACCGCCTTCGCCGTCGGC
GCCGAGGCCGTTTTGCATGACTACCTGCTGCCCCAGGATGTGATCACCGCCTGCGCCCACCACCGCATCACCGGGCTGAC
CGGGGTTCCGCCGCTCTGGGCCCAACTCGCCCGCCTCGACTGGCCGCCCGCCGCCACCGCCTCCTTGCGTTTTCTCGCCA
GCACCGGCGGAGCGATGCCCGCAGCGGTGCTGGCCCGCCTGCGCGCCCTGGCGCCCCTGGCGCGCATCCATCTGATGTAT
GGCCTGACCGAGGCTTTCCGCTCGACGACGCTCGATCCAGAGCGCGTTGGCCGCAAACCCGGCTCGGTCGGCCGGGCGGT
CGCCAATGCCGAGGTTCTGGTGCTGCACCCCGATGGCGGCCGCTGCGCCCCGAACGAGATCGGCGAGATCGTCCATCGCG
GCGCCTTCGTCGCCAAGGGCTATTGGAACGATCCCGAGGGCACGGCGCGGCGCTTCCGCCCGATCCCCCCCCACCCCGGC
GAGCCGTCGCGCCCGGGCCTGGCCGTCTGGTCGGGGGATTTGGGCTGGCAAGACGACGAGGGCGATTTGACCATCGTCGG
CCGCTCGGAAGGGCTGATCAAGACCTCGGGCTATCGGGTCAGCCCGACCGAGATCGAGGCGCCGGCCCATGCCAGCGGCC
TGATCGAAGACGCCGTGGCCTTCGGCCTGCCCGACCCGCTTCTGGGCGAGCGGCTGGCCCTGGTGGTCACCGCCCCGCCC
GGCGGCGCGCCCGTCGACCTCGACGCCTTGCGCCGCCATCTGCGCGCCCAGTTGCCAGCCTATCTGGTACCGGCCCTGCT
GACCCAGGTGGACAGCCTGCCGCGCACGGCCAGCGGCAAGGCCGATCGGGCGGCGGCGCGGGCGAGGATGCTCAAGGACC
AGGAGTCTCCGCCATGA

Upstream 100 bases:

>100_bases
TGCGCGCCGTGGACGAGCCTTCCGCCGACAGGGACGACAAGCCCCGCTCCCTCCCGCCGCGCCGCCCGACCCGCCCCGGC
GCCGGAGGGGTTGGCCACCC

Downstream 100 bases:

>100_bases
CCGCCTCCCCGCCCCCCGGCTTCGGCGTGAGCGGAAGCGACTTGACAATCGGCGGCCAAAGGGTGTCGCACCTGGCGCGG
ATCGCCGGGGGCACGCCGTT

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MRALFGLSDLLARACDHTPAATALSQAGAISRSAPPCLTYEGLFNRVCRLASGFKALGLRPGERVAVIAEKRFDAVAAMF
AVAHAGGVFVPINPVLKSPQIVHILADSAAKILVAPALRLALLDHTPPPSLTTLLRFGDPAPASPDPPPGGESLDLDALA
GAGDPIAAHPVVDDDPACFFYTSGSTGLPKAVVVTHRNLIAGAQSVASRLDNRPDDRLLAALPLGFDAGFSQLTTAFAVG
AEAVLHDYLLPQDVITACAHHRITGLTGVPPLWAQLARLDWPPAATASLRFLASTGGAMPAAVLARLRALAPLARIHLMY
GLTEAFRSTTLDPERVGRKPGSVGRAVANAEVLVLHPDGGRCAPNEIGEIVHRGAFVAKGYWNDPEGTARRFRPIPPHPG
EPSRPGLAVWSGDLGWQDDEGDLTIVGRSEGLIKTSGYRVSPTEIEAPAHASGLIEDAVAFGLPDPLLGERLALVVTAPP
GGAPVDLDALRRHLRAQLPAYLVPALLTQVDSLPRTASGKADRAAARARMLKDQESPP

Sequences:

>Translated_538_residues
MRALFGLSDLLARACDHTPAATALSQAGAISRSAPPCLTYEGLFNRVCRLASGFKALGLRPGERVAVIAEKRFDAVAAMF
AVAHAGGVFVPINPVLKSPQIVHILADSAAKILVAPALRLALLDHTPPPSLTTLLRFGDPAPASPDPPPGGESLDLDALA
GAGDPIAAHPVVDDDPACFFYTSGSTGLPKAVVVTHRNLIAGAQSVASRLDNRPDDRLLAALPLGFDAGFSQLTTAFAVG
AEAVLHDYLLPQDVITACAHHRITGLTGVPPLWAQLARLDWPPAATASLRFLASTGGAMPAAVLARLRALAPLARIHLMY
GLTEAFRSTTLDPERVGRKPGSVGRAVANAEVLVLHPDGGRCAPNEIGEIVHRGAFVAKGYWNDPEGTARRFRPIPPHPG
EPSRPGLAVWSGDLGWQDDEGDLTIVGRSEGLIKTSGYRVSPTEIEAPAHASGLIEDAVAFGLPDPLLGERLALVVTAPP
GGAPVDLDALRRHLRAQLPAYLVPALLTQVDSLPRTASGKADRAAARARMLKDQESPP
>Mature_538_residues
MRALFGLSDLLARACDHTPAATALSQAGAISRSAPPCLTYEGLFNRVCRLASGFKALGLRPGERVAVIAEKRFDAVAAMF
AVAHAGGVFVPINPVLKSPQIVHILADSAAKILVAPALRLALLDHTPPPSLTTLLRFGDPAPASPDPPPGGESLDLDALA
GAGDPIAAHPVVDDDPACFFYTSGSTGLPKAVVVTHRNLIAGAQSVASRLDNRPDDRLLAALPLGFDAGFSQLTTAFAVG
AEAVLHDYLLPQDVITACAHHRITGLTGVPPLWAQLARLDWPPAATASLRFLASTGGAMPAAVLARLRALAPLARIHLMY
GLTEAFRSTTLDPERVGRKPGSVGRAVANAEVLVLHPDGGRCAPNEIGEIVHRGAFVAKGYWNDPEGTARRFRPIPPHPG
EPSRPGLAVWSGDLGWQDDEGDLTIVGRSEGLIKTSGYRVSPTEIEAPAHASGLIEDAVAFGLPDPLLGERLALVVTAPP
GGAPVDLDALRRHLRAQLPAYLVPALLTQVDSLPRTASGKADRAAARARMLKDQESPP

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=532, Percent_Identity=23.4962406015038, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI187761345, Length=536, Percent_Identity=27.4253731343284, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI187761343, Length=536, Percent_Identity=27.4253731343284, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI58082049, Length=498, Percent_Identity=25.5020080321285, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI122937307, Length=535, Percent_Identity=25.7943925233645, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI42544132, Length=493, Percent_Identity=23.5294117647059, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI157311624, Length=498, Percent_Identity=25.1004016064257, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI157311622, Length=498, Percent_Identity=25.1004016064257, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI115511026, Length=514, Percent_Identity=25.2918287937743, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI38505220, Length=493, Percent_Identity=25.1521298174442, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI40807357, Length=544, Percent_Identity=24.4485294117647, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI13325057, Length=513, Percent_Identity=24.9512670565302, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI13236579, Length=494, Percent_Identity=24.8987854251012, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI38524616, Length=510, Percent_Identity=24.1176470588235, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI42794754, Length=196, Percent_Identity=29.0816326530612, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI42794752, Length=196, Percent_Identity=29.0816326530612, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI145693145, Length=505, Percent_Identity=27.9207920792079, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1788107, Length=507, Percent_Identity=26.6272189349112, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI221142682, Length=536, Percent_Identity=26.4925373134328, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786801, Length=544, Percent_Identity=26.6544117647059, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1786810, Length=504, Percent_Identity=25.3968253968254, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789201, Length=368, Percent_Identity=25.2717391304348, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1788595, Length=417, Percent_Identity=26.378896882494, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI32563687, Length=498, Percent_Identity=25.9036144578313, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17560308, Length=529, Percent_Identity=24.952741020794, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17558820, Length=516, Percent_Identity=24.4186046511628, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17531443, Length=513, Percent_Identity=24.5614035087719, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71994703, Length=546, Percent_Identity=22.8937728937729, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71994694, Length=546, Percent_Identity=22.8937728937729, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71994690, Length=546, Percent_Identity=22.8937728937729, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17559526, Length=555, Percent_Identity=24.8648648648649, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17538037, Length=513, Percent_Identity=23.3918128654971, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI115534440, Length=518, Percent_Identity=24.3243243243243, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17557194, Length=502, Percent_Identity=23.3067729083665, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17560140, Length=496, Percent_Identity=22.5806451612903, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=366, Percent_Identity=23.224043715847, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=353, Percent_Identity=27.4787535410765, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319264, Length=538, Percent_Identity=23.4200743494424, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24581924, Length=531, Percent_Identity=25.8003766478343, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI21355181, Length=501, Percent_Identity=26.7465069860279, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI19922652, Length=542, Percent_Identity=25.2767527675277, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24648260, Length=504, Percent_Identity=25.5952380952381, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21356441, Length=522, Percent_Identity=23.7547892720307, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24656500, Length=512, Percent_Identity=24.8046875, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24653035, Length=516, Percent_Identity=24.8062015503876, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281365686, Length=360, Percent_Identity=23.6111111111111, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI21358303, Length=560, Percent_Identity=21.9642857142857, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24648253, Length=524, Percent_Identity=22.9007633587786, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI20130357, Length=517, Percent_Identity=24.1779497098646, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648255, Length=524, Percent_Identity=22.9007633587786, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24583465, Length=542, Percent_Identity=22.6937269372694, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24583463, Length=542, Percent_Identity=22.6937269372694, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281364798, Length=542, Percent_Identity=22.6937269372694, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI161076582, Length=523, Percent_Identity=24.0917782026769, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI18859661, Length=491, Percent_Identity=25.2545824847251, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21356947, Length=515, Percent_Identity=23.1067961165049, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648257, Length=371, Percent_Identity=25.3369272237197, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 56309; Mature: 56309

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00105 AA_TRANSFER_CLASS_1 ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALFGLSDLLARACDHTPAATALSQAGAISRSAPPCLTYEGLFNRVCRLASGFKALGLR
CCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHCCCHHHCCC
PGERVAVIAEKRFDAVAAMFAVAHAGGVFVPINPVLKSPQIVHILADSAAKILVAPALRL
CCCEEEEEEHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCEEEEECCCCHHHHHHHHHHH
ALLDHTPPPSLTTLLRFGDPAPASPDPPPGGESLDLDALAGAGDPIAAHPVVDDDPACFF
HHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEECCCCCCCCCEEE
YTSGSTGLPKAVVVTHRNLIAGAQSVASRLDNRPDDRLLAALPLGFDAGFSQLTTAFAVG
EECCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
AEAVLHDYLLPQDVITACAHHRITGLTGVPPLWAQLARLDWPPAATASLRFLASTGGAMP
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCH
AAVLARLRALAPLARIHLMYGLTEAFRSTTLDPERVGRKPGSVGRAVANAEVLVLHPDGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHCCCEEEEEECCCC
RCAPNEIGEIVHRGAFVAKGYWNDPEGTARRFRPIPPHPGEPSRPGLAVWSGDLGWQDDE
CCCHHHHHHHHHCCCEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
GDLTIVGRSEGLIKTSGYRVSPTEIEAPAHASGLIEDAVAFGLPDPLLGERLALVVTAPP
CCEEEEECCCCEEEECCEEECCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCEEEEEECCC
GGAPVDLDALRRHLRAQLPAYLVPALLTQVDSLPRTASGKADRAAARARMLKDQESPP
CCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRALFGLSDLLARACDHTPAATALSQAGAISRSAPPCLTYEGLFNRVCRLASGFKALGLR
CCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHCCCHHHCCC
PGERVAVIAEKRFDAVAAMFAVAHAGGVFVPINPVLKSPQIVHILADSAAKILVAPALRL
CCCEEEEEEHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCEEEEECCCCHHHHHHHHHHH
ALLDHTPPPSLTTLLRFGDPAPASPDPPPGGESLDLDALAGAGDPIAAHPVVDDDPACFF
HHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEECCCCCCCCCEEE
YTSGSTGLPKAVVVTHRNLIAGAQSVASRLDNRPDDRLLAALPLGFDAGFSQLTTAFAVG
EECCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
AEAVLHDYLLPQDVITACAHHRITGLTGVPPLWAQLARLDWPPAATASLRFLASTGGAMP
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCH
AAVLARLRALAPLARIHLMYGLTEAFRSTTLDPERVGRKPGSVGRAVANAEVLVLHPDGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHCCCEEEEEECCCC
RCAPNEIGEIVHRGAFVAKGYWNDPEGTARRFRPIPPHPGEPSRPGLAVWSGDLGWQDDE
CCCHHHHHHHHHCCCEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
GDLTIVGRSEGLIKTSGYRVSPTEIEAPAHASGLIEDAVAFGLPDPLLGERLALVVTAPP
CCEEEEECCCCEEEECCEEECCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCEEEEEECCC
GGAPVDLDALRRHLRAQLPAYLVPALLTQVDSLPRTASGKADRAAARARMLKDQESPP
CCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]