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The map label for this gene is pchF [H]

Identifier: 83594442

GI number: 83594442

Start: 3588701

End: 3590299

Strand: Reverse

Name: pchF [H]

Synonym: Rru_A3112

Alternate gene names: 83594442

Gene position: 3590299-3588701 (Counterclockwise)

Preceding gene: 83594443

Following gene: 83594441

Centisome position: 82.48

GC content: 68.98

Gene sequence:

>1599_bases
ATGCTGGAGGATCGGGTCTTGACCCTTCACGCCTTCCTTGAGGGCGCGCGCGCCCTTCTTCCCCCGGCCGCCATTCTTTT
CGGCGAGGCGGTGACCCCTTTTCTTTCCTCGACCAGCGGCCATCGCCGGCGGGTGCCGCTGCTCGTGCGGCCGACAAACA
CCGGCGAGGTTCAGGCCCTCGCCCGCCTTGCCACCACCTGCGCGGTGACGCTCTATCCGATCAGCAAGGGGCGCAACTGG
GGCCTGGGCTCGCGGCTGCCCGGCTGCGAGGATTGCGTGATCCTCGATCTGGGCGGGCTCGACCGCATCAATGCCATCGA
TGAACGGTTCGGCATCGCCGTCATCGAACCCGGGGTGACGCAGGCCGCCCTGGCCGACGCTCTGGCGGCGCGGGGCTCGG
CCTTCTTCCTTGATGTGACCGGATCGGGGCGCGAGACCAGCGTGCTTGGCAATACCCTGGAACGCGGGGTGGCCTATAAT
TCGCTGCGCGCCGAATTGGTGCAGTCGCTCGAGGTGGTCCTGGCCGATGGCAGCTTGCTGCGCACCGGCTTCGCCCATTA
TCCGACCAGCCGCCTGGGCGGGCTCAGCCGCTTCGCCCCCGGGCCCGATCTGAGCGGGCTGTTCGTTCAATCCAACCTGG
GCATCGTCGTTGGCGGAGCGGTGGCGCTGTTGCCAAGGCCCGAGCGCCAGATGACCTTCATGGTCTCGGTGAAGGACGAG
GCGCGGCTTCCCGCGTTCTTCGACGCCCTGCGGGCCCTGCGCCGCGAGGGAACCCTGAGCAGCGTCGTTCATGTCGGCAA
CCGTCGGCGCAGCGAAATCACCCTGACGCCGCTGGTCCATGCCGAGATGGCGGCGCGCGGGCGCGATCCGACGCGGGCCG
AGGCCCAAAAGCTGACCGATCGCTTCCTGACCGGCCGCTGGAGCGCCATCGGCTCGGTGATGGGGCCGGCCGCCCAGGTT
CGGGTAGCGCGCCGCCGCATCGCCCGGGCTTTGGGCGGCCTTGGCGCCGTGCGCTTCCTGTCGCCGGGCTTTCGCCGCTT
CGCCAAGGCGCTCAGCGCCCGGATCCCGGGGCTGGGCGACGTCAATTGTTTCCTCTGCGCCGTCGATCCCTTGCTTGACC
TCACCAGCGGCCGGCCGACCAACGCGGCGCTGCACAGCACCTATTGGCCCCATGCCGATCAATCCGAGGCGGCCGCCGAT
CCCGATCGCGGGCCGGGCGGCATCGTCTTCGCCGCCCCCGTGGTGCCCTTGGACGGCGCGGCGGTGCGCGAGGCCGTCGA
CCTGACCTATGAGCTTTGCCGCGCCCACGGCTTCGAAGCGGCGATCACCCTTAATCTGATGAACGACCGCACCCTGGAAG
GCGTGGTCAGCATCGATTTTCGCCGCGACGACCCCGAGAACTTGGCCGCCGCCCATCGCTGCCTGCGGGCGCTCAACCAG
GGTTATGTGGAAAACGGCTTCACCCCCTATCGGGTCGATATCGATTCGATGGATCTGGTCGTCGATCCGGCCGATCCCTT
CTGGGCCACGGTGTCGCGCCTCAAGCAGGCCCTGGATCCGGCGGGGGTGGTGGCGCCGGGCCGCTATTGCCCGCCATGA

Upstream 100 bases:

>100_bases
AAATATCGGGCGATGATCGCCCTTTGGCGGCGACTGCCCCTTGCTGTGGCCAATACGCTTGGCCCTTGGATCAGCCGCCA
AGTCGGCTGATCGTCGTTTT

Downstream 100 bases:

>100_bases
GCAACGAAGGCGGACTGCTTGCCCTGCTGCGCGGCTGGTTCGGCGGCGTCTCGGCCAAGCGCTGGCGGACGCCGCCCTCG
ACCCGGCTTTACGCGGTGGG

Product: 4-cresol dehydrogenase

Products: NA

Alternate protein names: P-cresol methylhydroxylase; PCMH [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MLEDRVLTLHAFLEGARALLPPAAILFGEAVTPFLSSTSGHRRRVPLLVRPTNTGEVQALARLATTCAVTLYPISKGRNW
GLGSRLPGCEDCVILDLGGLDRINAIDERFGIAVIEPGVTQAALADALAARGSAFFLDVTGSGRETSVLGNTLERGVAYN
SLRAELVQSLEVVLADGSLLRTGFAHYPTSRLGGLSRFAPGPDLSGLFVQSNLGIVVGGAVALLPRPERQMTFMVSVKDE
ARLPAFFDALRALRREGTLSSVVHVGNRRRSEITLTPLVHAEMAARGRDPTRAEAQKLTDRFLTGRWSAIGSVMGPAAQV
RVARRRIARALGGLGAVRFLSPGFRRFAKALSARIPGLGDVNCFLCAVDPLLDLTSGRPTNAALHSTYWPHADQSEAAAD
PDRGPGGIVFAAPVVPLDGAAVREAVDLTYELCRAHGFEAAITLNLMNDRTLEGVVSIDFRRDDPENLAAAHRCLRALNQ
GYVENGFTPYRVDIDSMDLVVDPADPFWATVSRLKQALDPAGVVAPGRYCPP

Sequences:

>Translated_532_residues
MLEDRVLTLHAFLEGARALLPPAAILFGEAVTPFLSSTSGHRRRVPLLVRPTNTGEVQALARLATTCAVTLYPISKGRNW
GLGSRLPGCEDCVILDLGGLDRINAIDERFGIAVIEPGVTQAALADALAARGSAFFLDVTGSGRETSVLGNTLERGVAYN
SLRAELVQSLEVVLADGSLLRTGFAHYPTSRLGGLSRFAPGPDLSGLFVQSNLGIVVGGAVALLPRPERQMTFMVSVKDE
ARLPAFFDALRALRREGTLSSVVHVGNRRRSEITLTPLVHAEMAARGRDPTRAEAQKLTDRFLTGRWSAIGSVMGPAAQV
RVARRRIARALGGLGAVRFLSPGFRRFAKALSARIPGLGDVNCFLCAVDPLLDLTSGRPTNAALHSTYWPHADQSEAAAD
PDRGPGGIVFAAPVVPLDGAAVREAVDLTYELCRAHGFEAAITLNLMNDRTLEGVVSIDFRRDDPENLAAAHRCLRALNQ
GYVENGFTPYRVDIDSMDLVVDPADPFWATVSRLKQALDPAGVVAPGRYCPP
>Mature_532_residues
MLEDRVLTLHAFLEGARALLPPAAILFGEAVTPFLSSTSGHRRRVPLLVRPTNTGEVQALARLATTCAVTLYPISKGRNW
GLGSRLPGCEDCVILDLGGLDRINAIDERFGIAVIEPGVTQAALADALAARGSAFFLDVTGSGRETSVLGNTLERGVAYN
SLRAELVQSLEVVLADGSLLRTGFAHYPTSRLGGLSRFAPGPDLSGLFVQSNLGIVVGGAVALLPRPERQMTFMVSVKDE
ARLPAFFDALRALRREGTLSSVVHVGNRRRSEITLTPLVHAEMAARGRDPTRAEAQKLTDRFLTGRWSAIGSVMGPAAQV
RVARRRIARALGGLGAVRFLSPGFRRFAKALSARIPGLGDVNCFLCAVDPLLDLTSGRPTNAALHSTYWPHADQSEAAAD
PDRGPGGIVFAAPVVPLDGAAVREAVDLTYELCRAHGFEAAITLNLMNDRTLEGVVSIDFRRDDPENLAAAHRCLRALNQ
GYVENGFTPYRVDIDSMDLVVDPADPFWATVSRLKQALDPAGVVAPGRYCPP

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR006094
- InterPro:   IPR016170
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: =1.17.99.1 [H]

Molecular weight: Translated: 56948; Mature: 56948

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEDRVLTLHAFLEGARALLPPAAILFGEAVTPFLSSTSGHRRRVPLLVRPTNTGEVQAL
CCCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHH
ARLATTCAVTLYPISKGRNWGLGSRLPGCEDCVILDLGGLDRINAIDERFGIAVIEPGVT
HHHHHHHEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCEEEECCCCH
QAALADALAARGSAFFLDVTGSGRETSVLGNTLERGVAYNSLRAELVQSLEVVLADGSLL
HHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RTGFAHYPTSRLGGLSRFAPGPDLSGLFVQSNLGIVVGGAVALLPRPERQMTFMVSVKDE
HCCHHHCCHHHHCCCHHCCCCCCCCCEEEECCCCEEECCEEEECCCCCCCEEEEEEECCC
ARLPAFFDALRALRREGTLSSVVHVGNRRRSEITLTPLVHAEMAARGRDPTRAEAQKLTD
CCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHH
RFLTGRWSAIGSVMGPAAQVRVARRRIARALGGLGAVRFLSPGFRRFAKALSARIPGLGD
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHCCCCCCC
VNCFLCAVDPLLDLTSGRPTNAALHSTYWPHADQSEAAADPDRGPGGIVFAAPVVPLDGA
CCEEEEEHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCH
AVREAVDLTYELCRAHGFEAAITLNLMNDRTLEGVVSIDFRRDDPENLAAAHRCLRALNQ
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHC
GYVENGFTPYRVDIDSMDLVVDPADPFWATVSRLKQALDPAGVVAPGRYCPP
CHHHCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLEDRVLTLHAFLEGARALLPPAAILFGEAVTPFLSSTSGHRRRVPLLVRPTNTGEVQAL
CCCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHH
ARLATTCAVTLYPISKGRNWGLGSRLPGCEDCVILDLGGLDRINAIDERFGIAVIEPGVT
HHHHHHHEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCEEEECCCCH
QAALADALAARGSAFFLDVTGSGRETSVLGNTLERGVAYNSLRAELVQSLEVVLADGSLL
HHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RTGFAHYPTSRLGGLSRFAPGPDLSGLFVQSNLGIVVGGAVALLPRPERQMTFMVSVKDE
HCCHHHCCHHHHCCCHHCCCCCCCCCEEEECCCCEEECCEEEECCCCCCCEEEEEEECCC
ARLPAFFDALRALRREGTLSSVVHVGNRRRSEITLTPLVHAEMAARGRDPTRAEAQKLTD
CCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHH
RFLTGRWSAIGSVMGPAAQVRVARRRIARALGGLGAVRFLSPGFRRFAKALSARIPGLGD
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHCCCCCCC
VNCFLCAVDPLLDLTSGRPTNAALHSTYWPHADQSEAAADPDRGPGGIVFAAPVVPLDGA
CCEEEEEHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCH
AVREAVDLTYELCRAHGFEAAITLNLMNDRTLEGVVSIDFRRDDPENLAAAHRCLRALNQ
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHC
GYVENGFTPYRVDIDSMDLVVDPADPFWATVSRLKQALDPAGVVAPGRYCPP
CHHHCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7929007; 10565539; 3790500; 1846290; 10623531 [H]