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The map label for this gene is asnB [H]

Identifier: 83594269

GI number: 83594269

Start: 3384570

End: 3386330

Strand: Reverse

Name: asnB [H]

Synonym: Rru_A2937

Alternate gene names: 83594269

Gene position: 3386330-3384570 (Counterclockwise)

Preceding gene: 83594270

Following gene: 83594268

Centisome position: 77.8

GC content: 69.45

Gene sequence:

>1761_bases
ATGTGTGGCATAGCGGGCATGGTGACCAAACTCGGGCGGAGCGTGCCCGAAGGACTGGCCGACCGATTCGCGCAAAAGCT
GGCCCATCGCGGGCCCGATGGCAAAGGGGTGTTCGAGGCGCCCGGTGTGCTGCTGGTTCAGGCCCGGCTGGCGATCATCG
ATCTGTTGACCGGCGATCAGCCGCTGCATGGCCCCGACGGACTGGTGCTGGTGGCCAATGGCGAGGTCTATAACTACCGC
GAACTGCGCGCCGAATTGGGCGAGGGGGTGTTCCTGACCCGCTCGGACTGCGAACCGCCCTTGCACCTTTACGCCCGTCG
CGGGCTGGCCGGGTTCGATGTGCTGCGCGGCATGTATGCCATCGCCCTGTGGGATCCGCCGCGCCGCCGGCTGGTGCTGT
GCCGCGATCCCTTTGGCATCAAGCCGCTTTATTACGTGGAAACCAACGATGGCGTGGCCTTCGCCAGCGAGCCCCAGGCG
CTGATCGGCGCCGGTCTGCTGCCCGCCCGGGAAGACGCCGCCGCCGTCGACGAGCTTTTGCAGCTGCAGTTCACCTGCGG
GGCGCGGACGATCTTCCAGGGGATCCGCCGGGTTTTGCCCGGGGAAACCCTGGTCTTCGAAGACGCCCAATTGGTCGAGC
GCCACCGGCGCCCGGCGCTGCCCGCCGTCCGCGAGCGGGTCAGCCCGGCCGATCCTTTGGCCCTGCTGGACCATGTGCTT
GAAGACAGCCTTGATCTTCATCAGCGCGCCGACGTGCCCTATGGGCTGTTCCTGTCGGGCGGCATCGATTCATCGTCGGT
GCTGGCGATGATGGCCCGGCTCAACGAACGCCCGGTGCTGGCCTTCACCGCCGGCTTTCCCGGCACCGAGGTCGCCGACG
AGCGGGCCGATGCCCGGGCGGCGACCAGGGCGGTTGGCGCCCGGCATGTGGAGGTGGCGGTCAGCGAGGGCGATTTCCTG
GAGCATCTGCCGGCGATCGCCGCCGCCGTCGATGATCCGGTGGCCGATTACGCCATCGTCCCAACCTGGCTGCTCGCCCG
CGAGGCGGCGAAATCGGTCAAGGTCGTGCTGTGCGGCGAGGGTGGCGACGAACTGCTGGGCGGCTATGGCCGCTATCGTA
GCGCCATGCGCCCCTGGCCGCTGGCCCGTCCCTTGCGCCGCAAGGGCCTGCTTGATGGCCTGGGGCTGCTGCGCGGCGGC
GGTGGACGAAACTGGCGCGCCGGCATCGCCCGGGCCGAGGTCGAGGCCCGGGCCCAGGGCGGAAGCCGGCTGATCCGCGC
CCAGCGCGTTGATTGCGCCGATTGGCTGCCCCACGACCTGCTCAACAAGCTTGACCGCTGCTTGATGGCCCATGGCCTGG
AGGGGCGGGTGCCCTTCGTCGATGTCGATGTCGCCCGCTTCTGTCTGCCGCTGGCCGATGGATTGAAAGTGCGCGACGGC
ATGGGCAAATGGATCTTGCGCTCCTGGCTGGAACGCACCCTGCCCGAGGCCCGGCCCTTCGCCCCCAAACGCGGCTTCAG
CGTGCCGGTCGGCGAATGGATGAGCCGCCATGGCGATCGCCTGGGGCCGCTGGTCGCCGCCTCGCCGGCGGTGGCGGCGC
GGTGCCACGGCGAGGCCGTGCGCGCCCTGTTCACCCATCCGCGCAAGGAGACGGCGCTCGCCGCCTGGATCTTGCTTTAC
TATGCGCTGTGGCACCGCCGCCATATCGTCGGGCTGGCGCCCGAGGGCGATGTGTTCCACTGCCTGTCCACCGCCGCCTG
A

Upstream 100 bases:

>100_bases
GGAGAAGGTGGTGGCGAGCTACCGGAGTTTTTTTGAAACCATCGTCGCCGAGCGCTCCGGCGCCGTCGACCGTGGGTGAG
CGGACGGGGACCGGCGCGGC

Downstream 100 bases:

>100_bases
GGCGGCCGGCGGGGAGATCTTTGGCATCCCTCCTTTGAAGAAAAAGACCAAGAGCGCGACGCCTTTTTCGAACCGAAGAC
GCTATCTGTGGAGAGACGAG

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MCGIAGMVTKLGRSVPEGLADRFAQKLAHRGPDGKGVFEAPGVLLVQARLAIIDLLTGDQPLHGPDGLVLVANGEVYNYR
ELRAELGEGVFLTRSDCEPPLHLYARRGLAGFDVLRGMYAIALWDPPRRRLVLCRDPFGIKPLYYVETNDGVAFASEPQA
LIGAGLLPAREDAAAVDELLQLQFTCGARTIFQGIRRVLPGETLVFEDAQLVERHRRPALPAVRERVSPADPLALLDHVL
EDSLDLHQRADVPYGLFLSGGIDSSSVLAMMARLNERPVLAFTAGFPGTEVADERADARAATRAVGARHVEVAVSEGDFL
EHLPAIAAAVDDPVADYAIVPTWLLAREAAKSVKVVLCGEGGDELLGGYGRYRSAMRPWPLARPLRRKGLLDGLGLLRGG
GGRNWRAGIARAEVEARAQGGSRLIRAQRVDCADWLPHDLLNKLDRCLMAHGLEGRVPFVDVDVARFCLPLADGLKVRDG
MGKWILRSWLERTLPEARPFAPKRGFSVPVGEWMSRHGDRLGPLVAASPAVAARCHGEAVRALFTHPRKETALAAWILLY
YALWHRRHIVGLAPEGDVFHCLSTAA

Sequences:

>Translated_586_residues
MCGIAGMVTKLGRSVPEGLADRFAQKLAHRGPDGKGVFEAPGVLLVQARLAIIDLLTGDQPLHGPDGLVLVANGEVYNYR
ELRAELGEGVFLTRSDCEPPLHLYARRGLAGFDVLRGMYAIALWDPPRRRLVLCRDPFGIKPLYYVETNDGVAFASEPQA
LIGAGLLPAREDAAAVDELLQLQFTCGARTIFQGIRRVLPGETLVFEDAQLVERHRRPALPAVRERVSPADPLALLDHVL
EDSLDLHQRADVPYGLFLSGGIDSSSVLAMMARLNERPVLAFTAGFPGTEVADERADARAATRAVGARHVEVAVSEGDFL
EHLPAIAAAVDDPVADYAIVPTWLLAREAAKSVKVVLCGEGGDELLGGYGRYRSAMRPWPLARPLRRKGLLDGLGLLRGG
GGRNWRAGIARAEVEARAQGGSRLIRAQRVDCADWLPHDLLNKLDRCLMAHGLEGRVPFVDVDVARFCLPLADGLKVRDG
MGKWILRSWLERTLPEARPFAPKRGFSVPVGEWMSRHGDRLGPLVAASPAVAARCHGEAVRALFTHPRKETALAAWILLY
YALWHRRHIVGLAPEGDVFHCLSTAA
>Mature_586_residues
MCGIAGMVTKLGRSVPEGLADRFAQKLAHRGPDGKGVFEAPGVLLVQARLAIIDLLTGDQPLHGPDGLVLVANGEVYNYR
ELRAELGEGVFLTRSDCEPPLHLYARRGLAGFDVLRGMYAIALWDPPRRRLVLCRDPFGIKPLYYVETNDGVAFASEPQA
LIGAGLLPAREDAAAVDELLQLQFTCGARTIFQGIRRVLPGETLVFEDAQLVERHRRPALPAVRERVSPADPLALLDHVL
EDSLDLHQRADVPYGLFLSGGIDSSSVLAMMARLNERPVLAFTAGFPGTEVADERADARAATRAVGARHVEVAVSEGDFL
EHLPAIAAAVDDPVADYAIVPTWLLAREAAKSVKVVLCGEGGDELLGGYGRYRSAMRPWPLARPLRRKGLLDGLGLLRGG
GGRNWRAGIARAEVEARAQGGSRLIRAQRVDCADWLPHDLLNKLDRCLMAHGLEGRVPFVDVDVARFCLPLADGLKVRDG
MGKWILRSWLERTLPEARPFAPKRGFSVPVGEWMSRHGDRLGPLVAASPAVAARCHGEAVRALFTHPRKETALAAWILLY
YALWHRRHIVGLAPEGDVFHCLSTAA

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=540, Percent_Identity=26.1111111111111, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI168229252, Length=540, Percent_Identity=26.1111111111111, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI168229250, Length=540, Percent_Identity=26.1111111111111, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI296010848, Length=515, Percent_Identity=26.2135922330097, Blast_Score=110, Evalue=4e-24,
Organism=Escherichia coli, GI1786889, Length=536, Percent_Identity=28.9179104477612, Blast_Score=171, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI25147557, Length=540, Percent_Identity=26.8518518518519, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71993933, Length=540, Percent_Identity=26.8518518518519, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17560178, Length=547, Percent_Identity=25.0457038391225, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI25147560, Length=327, Percent_Identity=29.0519877675841, Blast_Score=96, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6321563, Length=618, Percent_Identity=26.537216828479, Blast_Score=157, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6325403, Length=623, Percent_Identity=26.645264847512, Blast_Score=156, Evalue=8e-39,
Organism=Drosophila melanogaster, GI45553209, Length=396, Percent_Identity=31.5656565656566, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 63815; Mature: 63815

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGMVTKLGRSVPEGLADRFAQKLAHRGPDGKGVFEAPGVLLVQARLAIIDLLTGDQ
CCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
PLHGPDGLVLVANGEVYNYRELRAELGEGVFLTRSDCEPPLHLYARRGLAGFDVLRGMYA
CCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCHHHHHHHHEE
IALWDPPRRRLVLCRDPFGIKPLYYVETNDGVAFASEPQALIGAGLLPAREDAAAVDELL
EEEECCCCCCEEEEECCCCCCEEEEEECCCCEEECCCCHHHHHCCCCCCCCHHHHHHHHH
QLQFTCGARTIFQGIRRVLPGETLVFEDAQLVERHRRPALPAVRERVSPADPLALLDHVL
HHHHHCCHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHH
EDSLDLHQRADVPYGLFLSGGIDSSSVLAMMARLNERPVLAFTAGFPGTEVADERADARA
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHH
ATRAVGARHVEVAVSEGDFLEHLPAIAAAVDDPVADYAIVPTWLLAREAAKSVKVVLCGE
HHHHHCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEECC
GGDELLGGYGRYRSAMRPWPLARPLRRKGLLDGLGLLRGGGGRNWRAGIARAEVEARAQG
CCHHHHCCHHHHHHCCCCCCCHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
GSRLIRAQRVDCADWLPHDLLNKLDRCLMAHGLEGRVPFVDVDVARFCLPLADGLKVRDG
CHHHHHHHCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCEECCC
MGKWILRSWLERTLPEARPFAPKRGFSVPVGEWMSRHGDRLGPLVAASPAVAARCHGEAV
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHH
RALFTHPRKETALAAWILLYYALWHRRHIVGLAPEGDVFHCLSTAA
HHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHCCC
>Mature Secondary Structure
MCGIAGMVTKLGRSVPEGLADRFAQKLAHRGPDGKGVFEAPGVLLVQARLAIIDLLTGDQ
CCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
PLHGPDGLVLVANGEVYNYRELRAELGEGVFLTRSDCEPPLHLYARRGLAGFDVLRGMYA
CCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCHHHHHHHHEE
IALWDPPRRRLVLCRDPFGIKPLYYVETNDGVAFASEPQALIGAGLLPAREDAAAVDELL
EEEECCCCCCEEEEECCCCCCEEEEEECCCCEEECCCCHHHHHCCCCCCCCHHHHHHHHH
QLQFTCGARTIFQGIRRVLPGETLVFEDAQLVERHRRPALPAVRERVSPADPLALLDHVL
HHHHHCCHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHH
EDSLDLHQRADVPYGLFLSGGIDSSSVLAMMARLNERPVLAFTAGFPGTEVADERADARA
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHH
ATRAVGARHVEVAVSEGDFLEHLPAIAAAVDDPVADYAIVPTWLLAREAAKSVKVVLCGE
HHHHHCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEECC
GGDELLGGYGRYRSAMRPWPLARPLRRKGLLDGLGLLRGGGGRNWRAGIARAEVEARAQG
CCHHHHCCHHHHHHCCCCCCCHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
GSRLIRAQRVDCADWLPHDLLNKLDRCLMAHGLEGRVPFVDVDVARFCLPLADGLKVRDG
CHHHHHHHCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCEECCC
MGKWILRSWLERTLPEARPFAPKRGFSVPVGEWMSRHGDRLGPLVAASPAVAARCHGEAV
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHH
RALFTHPRKETALAAWILLYYALWHRRHIVGLAPEGDVFHCLSTAA
HHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]