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The map label for this gene is ycgF [H]

Identifier: 83594266

GI number: 83594266

Start: 3379759

End: 3380517

Strand: Reverse

Name: ycgF [H]

Synonym: Rru_A2934

Alternate gene names: 83594266

Gene position: 3380517-3379759 (Counterclockwise)

Preceding gene: 83594267

Following gene: 83594264

Centisome position: 77.66

GC content: 64.43

Gene sequence:

>759_bases
ATGCCCGAGACGCCGGCCTGCGGGGGAGAGTGCAAGCGATCCCTGGGCTTCGAGTTCACCATGGCCTTTCAGCCCGTCGT
CGATCTGCCCACGCGGCGCATTTATGCCTATGAGGCCTTGGTGCGCGGCGTGGACGGCGAGGGGGCCCAGGCGGTTTTGG
CGCGGGTTACCCCCGAGACCCTTTACGCCTTCGATCAGGCCTGCCGAACCAAGGCGATCGAACTGGCGGTCGCCCAGGCG
ATGACCGCCCGCCTCAATATCAATTTCCTGCCCAATGCGGTCTACCACGCGGAAACCTGTTTGCGGCTGACCTTGGACAC
CGCCCGCCGCCTGGGCTTTCCTATGGACCGCATCACCTTCGAGTTCACCGAGAACGAAAGGGTGATGGATCGCGACCATC
TGCGCGCCATCGTCGCCACCTACCACCGCTTCGGCTTTCAGACGGCGCTGGATGATTTCGGAACGGGCTATGCCGGTTTG
GGGCTGCTGGCCGATTTCCAGCCCGATTGCCTGAAGATCGACCGTCTTCTGGTCGAGGGCATCGGCGAGGATCGGGTGCG
GCAGGCCATCGTCGGCGGACTTGTCGGCATCTGCGAGCGGCTGGCCATCGATGTGGTGGCCGAGGGCGTGGAAACCCTTG
GCGAATTGCGTGCCCTGGTCGCCATGGGCATCACCCGTTTCCAGGGCTACCTGTTCGCCCGGCCCAAGCTTGGCCGGTTG
ATCACCGAGGCCGAGATCGTCTTTCCCGACCTGGGGTGA

Upstream 100 bases:

>100_bases
AGGGATTCCGCCCCAGCGCCGGGGCGCCTGAGTCGCGCCCCGCTTTTGTCAGAGCCCCCCTTTTGTCAGAGCCCCCCTTT
TGTCAGAGAAGGACTGCGCG

Downstream 100 bases:

>100_bases
CCTATCCCGAGCCGGGATAACCCTTGGAAAACGCGCAAAAGAAAGGCCCCCGTGAGGGGGCCTGATCTTCCTTCGGATGG
CGCGAGTGACCGGACTTGAA

Product: diguanylate phosphodiesterase

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MPETPACGGECKRSLGFEFTMAFQPVVDLPTRRIYAYEALVRGVDGEGAQAVLARVTPETLYAFDQACRTKAIELAVAQA
MTARLNINFLPNAVYHAETCLRLTLDTARRLGFPMDRITFEFTENERVMDRDHLRAIVATYHRFGFQTALDDFGTGYAGL
GLLADFQPDCLKIDRLLVEGIGEDRVRQAIVGGLVGICERLAIDVVAEGVETLGELRALVAMGITRFQGYLFARPKLGRL
ITEAEIVFPDLG

Sequences:

>Translated_252_residues
MPETPACGGECKRSLGFEFTMAFQPVVDLPTRRIYAYEALVRGVDGEGAQAVLARVTPETLYAFDQACRTKAIELAVAQA
MTARLNINFLPNAVYHAETCLRLTLDTARRLGFPMDRITFEFTENERVMDRDHLRAIVATYHRFGFQTALDDFGTGYAGL
GLLADFQPDCLKIDRLLVEGIGEDRVRQAIVGGLVGICERLAIDVVAEGVETLGELRALVAMGITRFQGYLFARPKLGRL
ITEAEIVFPDLG
>Mature_251_residues
PETPACGGECKRSLGFEFTMAFQPVVDLPTRRIYAYEALVRGVDGEGAQAVLARVTPETLYAFDQACRTKAIELAVAQAM
TARLNINFLPNAVYHAETCLRLTLDTARRLGFPMDRITFEFTENERVMDRDHLRAIVATYHRFGFQTALDDFGTGYAGLG
LLADFQPDCLKIDRLLVEGIGEDRVRQAIVGGLVGICERLAIDVVAEGVETLGELRALVAMGITRFQGYLFARPKLGRLI
TEAEIVFPDLG

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=232, Percent_Identity=34.4827586206897, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788502, Length=231, Percent_Identity=30.7359307359307, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI87081980, Length=124, Percent_Identity=36.2903225806452, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=33.9285714285714, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790496, Length=227, Percent_Identity=25.9911894273128, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI226510982, Length=235, Percent_Identity=27.6595744680851, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082096, Length=220, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786507, Length=140, Percent_Identity=33.5714285714286, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87081845, Length=231, Percent_Identity=30.3030303030303, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081743, Length=229, Percent_Identity=27.0742358078603, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787055, Length=250, Percent_Identity=28.4, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788849, Length=162, Percent_Identity=29.0123456790123, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081921, Length=236, Percent_Identity=25.4237288135593, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 27840; Mature: 27708

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETPACGGECKRSLGFEFTMAFQPVVDLPTRRIYAYEALVRGVDGEGAQAVLARVTPET
CCCCCCCCHHHHHHCCCEEEEEECHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCHHH
LYAFDQACRTKAIELAVAQAMTARLNINFLPNAVYHAETCLRLTLDTARRLGFPMDRITF
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHEEE
EFTENERVMDRDHLRAIVATYHRFGFQTALDDFGTGYAGLGLLADFQPDCLKIDRLLVEG
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHC
IGEDRVRQAIVGGLVGICERLAIDVVAEGVETLGELRALVAMGITRFQGYLFARPKLGRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHH
ITEAEIVFPDLG
HHHHHEECCCCC
>Mature Secondary Structure 
PETPACGGECKRSLGFEFTMAFQPVVDLPTRRIYAYEALVRGVDGEGAQAVLARVTPET
CCCCCCCHHHHHHCCCEEEEEECHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCHHH
LYAFDQACRTKAIELAVAQAMTARLNINFLPNAVYHAETCLRLTLDTARRLGFPMDRITF
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHEEE
EFTENERVMDRDHLRAIVATYHRFGFQTALDDFGTGYAGLGLLADFQPDCLKIDRLLVEG
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHC
IGEDRVRQAIVGGLVGICERLAIDVVAEGVETLGELRALVAMGITRFQGYLFARPKLGRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHH
ITEAEIVFPDLG
HHHHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]