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The map label for this gene is oprM [H]

Identifier: 83594258

GI number: 83594258

Start: 3368729

End: 3370147

Strand: Reverse

Name: oprM [H]

Synonym: Rru_A2926

Alternate gene names: 83594258

Gene position: 3370147-3368729 (Counterclockwise)

Preceding gene: 83594259

Following gene: 83594257

Centisome position: 77.42

GC content: 68.36

Gene sequence:

>1419_bases
ATGCGCCTTCCTAGTTTGTCCGTGCTGATCCTGACCCTGTCGGCCTGCTCCCTCATTCCGCCGTTGGAGACGCCCAAACC
GCCGGTTCCGCCGGTCTTCCCTCAGAGCGATGGGGTGGCCGAGGGCGCGGGCGTGGCCGGATTGCCCCTTGCCGGGCTGG
TCCGCGACCCCCGCTTGCTGCGGATCGTCGATCTGGCCCTGGAGAACAACCGCGACCTGCGGCTGGCCCTTCTCAATGTC
GAGGAGGCCCGGTCGCAATATGGCGTTCAAGACGCGGATACCTTGCCCGGCGTCGACACCCAGGCCTCCTACAGCCGCGA
ACGGACCCCCGGTTCCGGGGGCAGATCGGCGACGGTCGCCGATCAGCATGGGATGACCTTGGGCTTGAACGCCTTCGAGA
TCGACTTGTTCGGTCGCGTCCGCGCCCTTTCCGCCGCCGCCCTGGAGCGCTATCTCGCCAGCGGCGAGGGCTACCGGGCG
GCGCGCCTCACCTTGGTCGGGGCGGTTGCCGACGCCTATTTCGCCGAGCGGCTGGCCGTTGAACAACGCGCCCTCGCCGA
ACGGACCTTGGCCGATTGGCGTCAATCCCTGGAACTGGCGCGCGATCTCCGGCGCAGTGGGCAGAGCGGCGAGGGGGATA
TGGTCCAGGCCGAGGCTCAGGTGGCTTTGGCCGAGGCCGATCTCCGGTCGCGGGAACGGGCCGTTTCCCAGGCCGGCAAC
GCCCTTCGCCTCGTCGTCGGAACCGATCTTCCCGCCGATCTGCCCGCCGCTTTGCGCCTGGACGAGCAACCGGTGACGAC
ACGGCTGCCCGCCGGTCTTCCGTCCGATCTTTTGTTGAACCGGCCCGACATCCGCCAAGCGGAGCGGACTCTCGCCGCCG
CCAACGCCGATGTCGGCGCCGCCCGGGCCGCGTTCTTCCCCCGACTCTCGCTTACCGCCGCCTTCGGCTTCGCCTCGCCG
ACCCTGGGGGCGCTGTTTCAGTCTTCGCACAACGCCTGGAACGTCTCCCCCCGTCTGGTTTTGCCGATTTTTGACGGTGG
GCGGCTTGATGCCGAATTGGATTTGGCGAAGGCCCGGGAAAAAGAGGCGGTCGCCCACTACGAAAAGGCGATCCAGACCG
CCTTTCGCGAAGTCGCCGATGGTCTTGCCGGAAGCGCCACGCTCGACAGACAGATCGAGGCGCAGAGCCGAAGCGTCGCG
GGGACGGCGCGCGCCGTCGCCTTGGCCGAGGCGCGCTATCGCGCCGGGCTGGAGGGCCGGCTGGCCTTGCTTGATGCCCA
ACGCCAGCTTCAAGCCGGTCAACAGACCCTGCTAAGCTTGCGCCGCGAGGAAATCGCCAACGCCGTGGCCCTCTACAAGG
CCTTGGGCGGCGGATCGACCGACGTCCGAGCGCCGGGAATGGGCGGGCCGGTCCAATGA

Upstream 100 bases:

>100_bases
CGTCCCCGTCCCCGTGCTGTCGATCCCAGCGCGCCCCGATCCTCCCCCGGTGAGGACAGCTTCTTGTATCGGGCCTGTTT
CTGGCTCAAGGACCAAGTCA

Downstream 100 bases:

>100_bases
ATGCGGACCAAGCGCCCGCGCCTTTGGAAAAGGACTCTGTGATCATGATGTCCGATCCCGGCGAGGGGCGATGGCGGCTG
CGCTTCTGGACGATTTTCGG

Product: RND efflux system, outer membrane lipoprotein, NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MRLPSLSVLILTLSACSLIPPLETPKPPVPPVFPQSDGVAEGAGVAGLPLAGLVRDPRLLRIVDLALENNRDLRLALLNV
EEARSQYGVQDADTLPGVDTQASYSRERTPGSGGRSATVADQHGMTLGLNAFEIDLFGRVRALSAAALERYLASGEGYRA
ARLTLVGAVADAYFAERLAVEQRALAERTLADWRQSLELARDLRRSGQSGEGDMVQAEAQVALAEADLRSRERAVSQAGN
ALRLVVGTDLPADLPAALRLDEQPVTTRLPAGLPSDLLLNRPDIRQAERTLAAANADVGAARAAFFPRLSLTAAFGFASP
TLGALFQSSHNAWNVSPRLVLPIFDGGRLDAELDLAKAREKEAVAHYEKAIQTAFREVADGLAGSATLDRQIEAQSRSVA
GTARAVALAEARYRAGLEGRLALLDAQRQLQAGQQTLLSLRREEIANAVALYKALGGGSTDVRAPGMGGPVQ

Sequences:

>Translated_472_residues
MRLPSLSVLILTLSACSLIPPLETPKPPVPPVFPQSDGVAEGAGVAGLPLAGLVRDPRLLRIVDLALENNRDLRLALLNV
EEARSQYGVQDADTLPGVDTQASYSRERTPGSGGRSATVADQHGMTLGLNAFEIDLFGRVRALSAAALERYLASGEGYRA
ARLTLVGAVADAYFAERLAVEQRALAERTLADWRQSLELARDLRRSGQSGEGDMVQAEAQVALAEADLRSRERAVSQAGN
ALRLVVGTDLPADLPAALRLDEQPVTTRLPAGLPSDLLLNRPDIRQAERTLAAANADVGAARAAFFPRLSLTAAFGFASP
TLGALFQSSHNAWNVSPRLVLPIFDGGRLDAELDLAKAREKEAVAHYEKAIQTAFREVADGLAGSATLDRQIEAQSRSVA
GTARAVALAEARYRAGLEGRLALLDAQRQLQAGQQTLLSLRREEIANAVALYKALGGGSTDVRAPGMGGPVQ
>Mature_472_residues
MRLPSLSVLILTLSACSLIPPLETPKPPVPPVFPQSDGVAEGAGVAGLPLAGLVRDPRLLRIVDLALENNRDLRLALLNV
EEARSQYGVQDADTLPGVDTQASYSRERTPGSGGRSATVADQHGMTLGLNAFEIDLFGRVRALSAAALERYLASGEGYRA
ARLTLVGAVADAYFAERLAVEQRALAERTLADWRQSLELARDLRRSGQSGEGDMVQAEAQVALAEADLRSRERAVSQAGN
ALRLVVGTDLPADLPAALRLDEQPVTTRLPAGLPSDLLLNRPDIRQAERTLAAANADVGAARAAFFPRLSLTAAFGFASP
TLGALFQSSHNAWNVSPRLVLPIFDGGRLDAELDLAKAREKEAVAHYEKAIQTAFREVADGLAGSATLDRQIEAQSRSVA
GTARAVALAEARYRAGLEGRLALLDAQRQLQAGQQTLLSLRREEIANAVALYKALGGGSTDVRAPGMGGPVQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=452, Percent_Identity=40.2654867256637, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI2367348, Length=466, Percent_Identity=28.755364806867, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50046; Mature: 50046

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPSLSVLILTLSACSLIPPLETPKPPVPPVFPQSDGVAEGAGVAGLPLAGLVRDPRLL
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHE
RIVDLALENNRDLRLALLNVEEARSQYGVQDADTLPGVDTQASYSRERTPGSGGRSATVA
EHHHHHHCCCCCEEEEEECHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
DQHGMTLGLNAFEIDLFGRVRALSAAALERYLASGEGYRAARLTLVGAVADAYFAERLAV
CCCCCEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
EQRALAERTLADWRQSLELARDLRRSGQSGEGDMVQAEAQVALAEADLRSRERAVSQAGN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCC
ALRLVVGTDLPADLPAALRLDEQPVTTRLPAGLPSDLLLNRPDIRQAERTLAAANADVGA
EEEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCH
ARAAFFPRLSLTAAFGFASPTLGALFQSSHNAWNVSPRLVLPIFDGGRLDAELDLAKARE
HHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHH
KEAVAHYEKAIQTAFREVADGLAGSATLDRQIEAQSRSVAGTARAVALAEARYRAGLEGR
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LALLDAQRQLQAGQQTLLSLRREEIANAVALYKALGGGSTDVRAPGMGGPVQ
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRLPSLSVLILTLSACSLIPPLETPKPPVPPVFPQSDGVAEGAGVAGLPLAGLVRDPRLL
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHE
RIVDLALENNRDLRLALLNVEEARSQYGVQDADTLPGVDTQASYSRERTPGSGGRSATVA
EHHHHHHCCCCCEEEEEECHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
DQHGMTLGLNAFEIDLFGRVRALSAAALERYLASGEGYRAARLTLVGAVADAYFAERLAV
CCCCCEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
EQRALAERTLADWRQSLELARDLRRSGQSGEGDMVQAEAQVALAEADLRSRERAVSQAGN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCC
ALRLVVGTDLPADLPAALRLDEQPVTTRLPAGLPSDLLLNRPDIRQAERTLAAANADVGA
EEEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCH
ARAAFFPRLSLTAAFGFASPTLGALFQSSHNAWNVSPRLVLPIFDGGRLDAELDLAKARE
HHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHH
KEAVAHYEKAIQTAFREVADGLAGSATLDRQIEAQSRSVAGTARAVALAEARYRAGLEGR
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LALLDAQRQLQAGQQTLLSLRREEIANAVALYKALGGGSTDVRAPGMGGPVQ
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]