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The map label for this gene is dnaG [H]

Identifier: 83594215

GI number: 83594215

Start: 3324658

End: 3326520

Strand: Reverse

Name: dnaG [H]

Synonym: Rru_A2883

Alternate gene names: 83594215

Gene position: 3326520-3324658 (Counterclockwise)

Preceding gene: 83594216

Following gene: 83594214

Centisome position: 76.42

GC content: 68.55

Gene sequence:

>1863_bases
ATGGCCTTGCCAAATGCCTTTCTCGATGAATTGCGCGCCCGGCTGACCCTGTCCTCGGTGGTCGGCCGGCGCGTGAAGCT
TGCCCGCAAGGGCCGCGAATTCCTTGGGCTCTGTCCCTTCCATAACGAAAAATCGCCCTCGTTCACCGTCAATGACGAGA
AGGGGTTCTTCCACTGCTTTGGCTGTGGCGCCCATGGCGACGTTATCGGATTCGAGATGCGCGCCGCCAACATCGCCTTT
CGCGAGGCCGTCGAACGGCTGTCGGCCGAGGCCGGGCTGGAGATGCCGCGCGAAACCGCCGAGGACCGCGATCGCTCGCG
TCAGGCCGCGAGCCACCGTGAGATCATGGAGATGGCCTGCGCCTTCTATCAGAAGGCCCTGCGCCTGCCCGAGGGACGCG
GCGCCATGGAGTATTTGCGCGGTCGCGGCCTGGATGACGCCACCCTGGCGCGGTTCCGCCTGGGGTTTTCGCCCCAGGGC
GGGGCGCTGAAGGCGGCGCTGGCCCGCGAGGGCGTGGACGAGCAGGCGATGGTCGACCTTGGGCTGATCCTGCGCTCCGA
CGACGGCCGGCCCGCCTTCGATTACTTCCGTGGGCGGGTGATGTTCCCGATCGGCGATCCGGCCGGGCGGGTGGTGGCCT
TCGGCGCCCGCATCCTCGGCGAGGGGCAGCCCAAATACCTGAATTCGCCCGATACCCCGCTCTTTCACAAGGGCCACAGC
CTTTACAATCTGGGGCCGGCGCGCGAGGCGGCGCGCCAGAGCGGCGCCATCATCGTCGCCGAAGGCTATATGGACGTCAT
CGCCCTGGCCCGCGCCGGGCTGGGTTGGGCGGTGGCCCCCCTGGGCACGGCGCTGACCGAGGCCCAGATGGAGGCGCTGT
GGCGCACCATCCCCGAACCGGTGCTGTGCTTCGATGGCGACGGCGCCGGGCGGCGGGCGGCGGCGCGGGCGATGGAACGG
GCCCTGCCGTTGATCGGCCCCGGGCGCTCGTTGCGCTTCGCCCTGTTGCCCGGTGGACGCGATCCCGACGATCTGTTGCG
CGAAGGCGGCCCCGTCGCCCTGCGTCAGGTGCTTGATGTGGCCGTGCCGTTTTCGGAAATGGCTTGGCGCGAGTTGAGCG
AGGGGCAGGCCCTGACCACCCCCGAGCAGCGCGCCGCCCTGGAGGCCGCCATCGACGCCTTCGTCGCCAAAATCGCCGAC
CGGTCGCTGCAGGGCCATTACCGTCAGGCGCTGCGCGACCGCTTCTGGACGGTATTGCGGGGCGACTCCGCGCGCTCACG
GGCCGAGCGGGCGCGCAAGGACCCCCGAAAGGAGCGCAAGGACGGTGGGCGCTGGCGCCCGGGCGACCGCCCGCCGGCGC
CCGGTCTGCCGGTCGCCGCCCCGGCCGATCCGGCGCGGTTGCGCGAACGCCTGCTGCTGGCGGCGGTCTTGAATCATCCC
GAGGTTTTCGATCATGTCGGCGAGCGCCTGGGGGCGATGATTCTGGGGGAGGGGGCGCTTGACTCCTTGCGACAGGACGT
CGTAATGATCCTCGGGGGTGGGAAAAGCCTTGACATGAATGGTCTTTGGAGCCAGTTGAGCGCCAGGGGATACGCGGCCG
ACGTGGCCGCCCTGACGCGGCCGGAACTGTTGATCCATGGGAAATTCGCACGCCCGGGCGCAGATCCGGGCTTGGTCAAG
GCCGGATGGGACCATACATATGATCTCCATCAACGCGGAGCGTTGCAGGCCGACTGTCAAAGGGCCAATGAGCGGTTGGG
AGAACACATGACTCAAGAGGCCCTTGAGCATTTCCTGGCCTTCAAGCAGCAGGACAAATGGGCCGACGAGCCGGTGCTCG
ATGGCCCGGTCCCCGAGGGATGA

Upstream 100 bases:

>100_bases
GATTTGCGCCGCTCCCAGACCCTCGAAAGCGGTTGCGCCGCGGATCTTGGGGCGAGGAAGCGGTTGCGCCGCGGATCTTG
GGGCGAGGAAAGGGCGTTTC

Downstream 100 bases:

>100_bases
GCCTCCAGGGGATCAGATCCCGGCTGATCGGACGGACGGTCCGGCGCCGGGACACGTGAGTTTTCCCTTGGAGGTCGCGG
ACTTGAGGGTCCGTGGATTT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 620; Mature: 619

Protein sequence:

>620_residues
MALPNAFLDELRARLTLSSVVGRRVKLARKGREFLGLCPFHNEKSPSFTVNDEKGFFHCFGCGAHGDVIGFEMRAANIAF
REAVERLSAEAGLEMPRETAEDRDRSRQAASHREIMEMACAFYQKALRLPEGRGAMEYLRGRGLDDATLARFRLGFSPQG
GALKAALAREGVDEQAMVDLGLILRSDDGRPAFDYFRGRVMFPIGDPAGRVVAFGARILGEGQPKYLNSPDTPLFHKGHS
LYNLGPAREAARQSGAIIVAEGYMDVIALARAGLGWAVAPLGTALTEAQMEALWRTIPEPVLCFDGDGAGRRAAARAMER
ALPLIGPGRSLRFALLPGGRDPDDLLREGGPVALRQVLDVAVPFSEMAWRELSEGQALTTPEQRAALEAAIDAFVAKIAD
RSLQGHYRQALRDRFWTVLRGDSARSRAERARKDPRKERKDGGRWRPGDRPPAPGLPVAAPADPARLRERLLLAAVLNHP
EVFDHVGERLGAMILGEGALDSLRQDVVMILGGGKSLDMNGLWSQLSARGYAADVAALTRPELLIHGKFARPGADPGLVK
AGWDHTYDLHQRGALQADCQRANERLGEHMTQEALEHFLAFKQQDKWADEPVLDGPVPEG

Sequences:

>Translated_620_residues
MALPNAFLDELRARLTLSSVVGRRVKLARKGREFLGLCPFHNEKSPSFTVNDEKGFFHCFGCGAHGDVIGFEMRAANIAF
REAVERLSAEAGLEMPRETAEDRDRSRQAASHREIMEMACAFYQKALRLPEGRGAMEYLRGRGLDDATLARFRLGFSPQG
GALKAALAREGVDEQAMVDLGLILRSDDGRPAFDYFRGRVMFPIGDPAGRVVAFGARILGEGQPKYLNSPDTPLFHKGHS
LYNLGPAREAARQSGAIIVAEGYMDVIALARAGLGWAVAPLGTALTEAQMEALWRTIPEPVLCFDGDGAGRRAAARAMER
ALPLIGPGRSLRFALLPGGRDPDDLLREGGPVALRQVLDVAVPFSEMAWRELSEGQALTTPEQRAALEAAIDAFVAKIAD
RSLQGHYRQALRDRFWTVLRGDSARSRAERARKDPRKERKDGGRWRPGDRPPAPGLPVAAPADPARLRERLLLAAVLNHP
EVFDHVGERLGAMILGEGALDSLRQDVVMILGGGKSLDMNGLWSQLSARGYAADVAALTRPELLIHGKFARPGADPGLVK
AGWDHTYDLHQRGALQADCQRANERLGEHMTQEALEHFLAFKQQDKWADEPVLDGPVPEG
>Mature_619_residues
ALPNAFLDELRARLTLSSVVGRRVKLARKGREFLGLCPFHNEKSPSFTVNDEKGFFHCFGCGAHGDVIGFEMRAANIAFR
EAVERLSAEAGLEMPRETAEDRDRSRQAASHREIMEMACAFYQKALRLPEGRGAMEYLRGRGLDDATLARFRLGFSPQGG
ALKAALAREGVDEQAMVDLGLILRSDDGRPAFDYFRGRVMFPIGDPAGRVVAFGARILGEGQPKYLNSPDTPLFHKGHSL
YNLGPAREAARQSGAIIVAEGYMDVIALARAGLGWAVAPLGTALTEAQMEALWRTIPEPVLCFDGDGAGRRAAARAMERA
LPLIGPGRSLRFALLPGGRDPDDLLREGGPVALRQVLDVAVPFSEMAWRELSEGQALTTPEQRAALEAAIDAFVAKIADR
SLQGHYRQALRDRFWTVLRGDSARSRAERARKDPRKERKDGGRWRPGDRPPAPGLPVAAPADPARLRERLLLAAVLNHPE
VFDHVGERLGAMILGEGALDSLRQDVVMILGGGKSLDMNGLWSQLSARGYAADVAALTRPELLIHGKFARPGADPGLVKA
GWDHTYDLHQRGALQADCQRANERLGEHMTQEALEHFLAFKQQDKWADEPVLDGPVPEG

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=418, Percent_Identity=42.1052631578947, Blast_Score=341, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 67777; Mature: 67646

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALPNAFLDELRARLTLSSVVGRRVKLARKGREFLGLCPFHNEKSPSFTVNDEKGFFHCF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCEEECCCCCEEEEE
GCGAHGDVIGFEMRAANIAFREAVERLSAEAGLEMPRETAEDRDRSRQAASHREIMEMAC
ECCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AFYQKALRLPEGRGAMEYLRGRGLDDATLARFRLGFSPQGGALKAALAREGVDEQAMVDL
HHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHH
GLILRSDDGRPAFDYFRGRVMFPIGDPAGRVVAFGARILGEGQPKYLNSPDTPLFHKGHS
HHEEECCCCCCHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEECCCC
LYNLGPAREAARQSGAIIVAEGYMDVIALARAGLGWAVAPLGTALTEAQMEALWRTIPEP
CCCCCHHHHHHHHCCCEEEECCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCC
VLCFDGDGAGRRAAARAMERALPLIGPGRSLRFALLPGGRDPDDLLREGGPVALRQVLDV
EEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHHH
AVPFSEMAWRELSEGQALTTPEQRAALEAAIDAFVAKIADRSLQGHYRQALRDRFWTVLR
HCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDSARSRAERARKDPRKERKDGGRWRPGDRPPAPGLPVAAPADPARLRERLLLAAVLNHP
CCCHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCH
EVFDHVGERLGAMILGEGALDSLRQDVVMILGGGKSLDMNGLWSQLSARGYAADVAALTR
HHHHHHHHHHHHHHCCCHHHHHHHHCEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHCC
PELLIHGKFARPGADPGLVKAGWDHTYDLHQRGALQADCQRANERLGEHMTQEALEHFLA
CCEEEEECCCCCCCCCCCEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FKQQDKWADEPVLDGPVPEG
HHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ALPNAFLDELRARLTLSSVVGRRVKLARKGREFLGLCPFHNEKSPSFTVNDEKGFFHCF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCEEECCCCCEEEEE
GCGAHGDVIGFEMRAANIAFREAVERLSAEAGLEMPRETAEDRDRSRQAASHREIMEMAC
ECCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AFYQKALRLPEGRGAMEYLRGRGLDDATLARFRLGFSPQGGALKAALAREGVDEQAMVDL
HHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHH
GLILRSDDGRPAFDYFRGRVMFPIGDPAGRVVAFGARILGEGQPKYLNSPDTPLFHKGHS
HHEEECCCCCCHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEECCCC
LYNLGPAREAARQSGAIIVAEGYMDVIALARAGLGWAVAPLGTALTEAQMEALWRTIPEP
CCCCCHHHHHHHHCCCEEEECCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCC
VLCFDGDGAGRRAAARAMERALPLIGPGRSLRFALLPGGRDPDDLLREGGPVALRQVLDV
EEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHHH
AVPFSEMAWRELSEGQALTTPEQRAALEAAIDAFVAKIADRSLQGHYRQALRDRFWTVLR
HCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDSARSRAERARKDPRKERKDGGRWRPGDRPPAPGLPVAAPADPARLRERLLLAAVLNHP
CCCHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCH
EVFDHVGERLGAMILGEGALDSLRQDVVMILGGGKSLDMNGLWSQLSARGYAADVAALTR
HHHHHHHHHHHHHHCCCHHHHHHHHCEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHCC
PELLIHGKFARPGADPGLVKAGWDHTYDLHQRGALQADCQRANERLGEHMTQEALEHFLA
CCEEEEECCCCCCCCCCCEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FKQQDKWADEPVLDGPVPEG
HHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]