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The map label for this gene is rpoA

Identifier: 83593996

GI number: 83593996

Start: 3094597

End: 3095619

Strand: Reverse

Name: rpoA

Synonym: Rru_A2664

Alternate gene names: 83593996

Gene position: 3095619-3094597 (Counterclockwise)

Preceding gene: 83593997

Following gene: 83593995

Centisome position: 71.12

GC content: 59.92

Gene sequence:

>1023_bases
ATGTCCGTGATCCAAAAGAATTGGCAGGAACTGATTAAGCCGAACAAGCTCATGATCGAGTTGGTCGGCGGATCCAAAAA
AGTCGCCACGGTCGTGGCCGAGCCGCTGGAGCGTGGATTCGGTTTGACCCTTGGCAACGCCCTGCGTCGCGTGTTGCTGT
CGTCCCTTCAGGGCGCGGCGGTGACCGCCATCCAGATCGAGGGCGTTCTGCACGAGTTCTCGTCGATCCCCGGCGTCCGC
GAGGATGTCACCGACGTGATCCTCAACATCAAGGGCTTGGCGCTCCTGATGCATTCCGAGGGTCCGCGCCGGATGACGCT
GAAGGCGAGCGGCCCCGGTGAGGTGACCGCCGCTCAGATCGAGATGGGGTCCGATATCGAGATCATGAATCCCGATCTGG
TGATCTGCCACCTCGACGAAGGCGCGACCTTGTCGATGGAGTTCACCGTCGGCATGGGCAAGGGCTATGTTCCCTCGACC
CAGAACCGTCCCGAGGACGCCCCGATCGGCTATATTCCGATCGACAGCATCTTCTCGCCGGTGACCAAGGTCTCCTATCG
CGTCGAGAACAGCCGTGTCGGTCAGGTCACCGACTATGACAAGCTGTCGATGGTGGTCGAGACCGATGGTTCGGTCGGTC
CCGAGGACGCGGTGGCTTTGGCCGCGCGCATTCTCCAGGACCAGCTTCAGCTGTTCATCAACTTCGAGGAGCCCCAGGCG
GTTTCTGAGGAGAAGAAGGACGACGAGCTGCCGTTCAACAAGAACCTGCTGCGCAAGGTCGACGAACTGGAGCTTTCGGT
CCGTTCGGCGAACTGCCTGAAGAACGACAACATCATCTATATCGGCGATCTGGTTCAGAAGACCGAGGCCGAGATGCTGC
GGACGCCGAATTTCGGCCGCAAGTCGCTCAACGAGATCAAGGAAGTGCTGGCCCAGATGGGGCTGCACCTGGGCATGGAA
ATTTCCAACTGGCCGCCCGAGAATATCGAGGATCTGGCCAAGAAGCTGGAAGAGCCCTACTGA

Upstream 100 bases:

>100_bases
GGGCCGCCGGAACCCCACCGAAGGGGTTCGGCGGTTCGTCCCTTGAGATCAGGGGCGGGCTTTCGGAAACAACCGCCGAC
CCCGATGCCAGGAAGCCGCG

Downstream 100 bases:

>100_bases
TCAGCTGATCCAAGCCGGGTCCCTGGAGATCGCCAGGGATCGGCCTGAAAGCGACCCGCGATAGGGCGGGTCGTCGTTCG
AATGCTTGACCGGAACCCTT

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MSVIQKNWQELIKPNKLMIELVGGSKKVATVVAEPLERGFGLTLGNALRRVLLSSLQGAAVTAIQIEGVLHEFSSIPGVR
EDVTDVILNIKGLALLMHSEGPRRMTLKASGPGEVTAAQIEMGSDIEIMNPDLVICHLDEGATLSMEFTVGMGKGYVPST
QNRPEDAPIGYIPIDSIFSPVTKVSYRVENSRVGQVTDYDKLSMVVETDGSVGPEDAVALAARILQDQLQLFINFEEPQA
VSEEKKDDELPFNKNLLRKVDELELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKSLNEIKEVLAQMGLHLGME
ISNWPPENIEDLAKKLEEPY

Sequences:

>Translated_340_residues
MSVIQKNWQELIKPNKLMIELVGGSKKVATVVAEPLERGFGLTLGNALRRVLLSSLQGAAVTAIQIEGVLHEFSSIPGVR
EDVTDVILNIKGLALLMHSEGPRRMTLKASGPGEVTAAQIEMGSDIEIMNPDLVICHLDEGATLSMEFTVGMGKGYVPST
QNRPEDAPIGYIPIDSIFSPVTKVSYRVENSRVGQVTDYDKLSMVVETDGSVGPEDAVALAARILQDQLQLFINFEEPQA
VSEEKKDDELPFNKNLLRKVDELELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKSLNEIKEVLAQMGLHLGME
ISNWPPENIEDLAKKLEEPY
>Mature_339_residues
SVIQKNWQELIKPNKLMIELVGGSKKVATVVAEPLERGFGLTLGNALRRVLLSSLQGAAVTAIQIEGVLHEFSSIPGVRE
DVTDVILNIKGLALLMHSEGPRRMTLKASGPGEVTAAQIEMGSDIEIMNPDLVICHLDEGATLSMEFTVGMGKGYVPSTQ
NRPEDAPIGYIPIDSIFSPVTKVSYRVENSRVGQVTDYDKLSMVVETDGSVGPEDAVALAARILQDQLQLFINFEEPQAV
SEEKKDDELPFNKNLLRKVDELELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKSLNEIKEVLAQMGLHLGMEI
SNWPPENIEDLAKKLEEPY

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=332, Percent_Identity=50, Blast_Score=319, Evalue=2e-88,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_RHORT (Q2RQY4)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_427748.1
- HSSP:   P20429
- ProteinModelPortal:   Q2RQY4
- STRING:   Q2RQY4
- GeneID:   3836099
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A2664
- NMPDR:   fig|1085.1.peg.314
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- PhylomeDB:   Q2RQY4
- ProtClustDB:   PRK05182
- BioCyc:   RRUB269796:RRU_A2664-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37454; Mature: 37322

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIQKNWQELIKPNKLMIELVGGSKKVATVVAEPLERGFGLTLGNALRRVLLSSLQGAA
CCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCE
VTAIQIEGVLHEFSSIPGVREDVTDVILNIKGLALLMHSEGPRRMTLKASGPGEVTAAQI
EEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCEEEEEE
EMGSDIEIMNPDLVICHLDEGATLSMEFTVGMGKGYVPSTQNRPEDAPIGYIPIDSIFSP
ECCCCEEEECCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHH
VTKVSYRVENSRVGQVTDYDKLSMVVETDGSVGPEDAVALAARILQDQLQLFINFEEPQA
HHHHHEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCH
VSEEKKDDELPFNKNLLRKVDELELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGR
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCH
KSLNEIKEVLAQMGLHLGMEISNWPPENIEDLAKKLEEPY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
SVIQKNWQELIKPNKLMIELVGGSKKVATVVAEPLERGFGLTLGNALRRVLLSSLQGAA
CHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCE
VTAIQIEGVLHEFSSIPGVREDVTDVILNIKGLALLMHSEGPRRMTLKASGPGEVTAAQI
EEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCEEEEEE
EMGSDIEIMNPDLVICHLDEGATLSMEFTVGMGKGYVPSTQNRPEDAPIGYIPIDSIFSP
ECCCCEEEECCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHH
VTKVSYRVENSRVGQVTDYDKLSMVVETDGSVGPEDAVALAARILQDQLQLFINFEEPQA
HHHHHEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCH
VSEEKKDDELPFNKNLLRKVDELELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGR
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCH
KSLNEIKEVLAQMGLHLGMEISNWPPENIEDLAKKLEEPY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA