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The map label for this gene is dosC [H]

Identifier: 83593926

GI number: 83593926

Start: 3015117

End: 3016865

Strand: Reverse

Name: dosC [H]

Synonym: Rru_A2594

Alternate gene names: 83593926

Gene position: 3016865-3015117 (Counterclockwise)

Preceding gene: 83593927

Following gene: 83593925

Centisome position: 69.31

GC content: 57.8

Gene sequence:

>1749_bases
ATGCTCGCCTTGACGCGCCTACTGCTTTTGGTCCTCGTGGTGGCCTGCGCCACGCCCGCTTTCGCCGAGGAGAGGGGGGC
AGATCACATCCTCGCCCGGGCTTTCCTCGAAGACCCGGCCGGTGTTCTGACGATCGACGAGGTCGCCCGGGGCGACTTCA
CCCCCTTCGGGCCGAACTTCTCGAAAGGCTATACCCAATCGGTTTATTGGGTGCGCCTGCTTGTCCGCGCCTCCGCCGCG
CCCGAGAAGACGGTGCTGTTCATCCGCCCAAGTTTTCTCAACGAGGTCCGCTTGTTCTACCGGGATCGCGCGTCTCCCGA
TGGATGGGCGACGCGGGTGAGCGGAAACCGCTATCCCTTTTCCGAGCGTGATCGCAAGAGCGTCGCATTGGGCTTCGTCG
TCGATGTGACGCATCCGCAAGAGGAATTCTATCTGCGGATAAAAACGAATACCCAAATGCAAATCGATGTTCAGGCGGTT
TCTCCCGACGTTGCGGCGGATACGGATTCGCGGCGCGACATGGTGATGGTGTTTTTCGTCACATCGATGATTCTTATGAT
ATTTTGGGGGGTCAACGAATATAGTTTTGATAGATATAGAATTTTTGCTCTTTTTTGCATTCATCAATTGGCGTTTACGC
TGTTTGGAATATCGGCGGTCGGCTATTTTTCTCCGTTTTGGGTTATGATTTTCCCTGGAATAGTTGATCGTGCTTTCTAT
TTTCTATATATGTCAATATCTTTTACATTGATTTTATTCTGCCGAGAGCTTTTTAAGCCTTATCAACCCCACCCTGTGCT
GGCAAAGGGGCTTGATTTGGCCCGCTGGGCCTTTCCGCTGCAAATCATAGCGCTGATCGCCGGCTATGATGCCCTGGCCG
TGATCAGCAATGCGCTTCTGATCAAGGGAGCGCTGTTCTATTTCGTGTTTGTCGCCTTTTCTTTGAAGGTCGATCTCGAT
CCGACGCGGAGGACCTTGAGGATTTTCTTCATCGGGATCGCCCTGATCAATCTGATCTTCTGGATGACGCCACGGGTTGG
CGGCGAGTTGAAATGGTTGGGGTTGAGCGCGGTTCAGGCCTTGGTGATCGACGGGTTCGTCATGGGGATTCTGTTCGCCT
GGCTGGCCCATGGGCGGGGCCGGCAAGTCCGCGAGGACGCCCAGCGCAAGATCCGGCTTGTCAAGGAAAGCTATCTTGCG
GAACGCGATCTGAAGGCCCGGGCCGAGCGGGAAGCCAGGGTCGATTATCTGACGGGCGTGTTAAACCGCCGCTCCTTTTT
CGAGTTGGCCGAGGGGGAATTGGCGCGATCAATCCGCTTTGGGCGGCCTTTGGCTGTTTTGATGATCGATATCGACTATT
TCAAAGCGGTCAATGACACCTGGGGGCACGGCGTCGGCGATGAGGTGTTGCAAAACGTCGCCGGGCTGATCGGCCAGACC
CTGCGCGGCGCCGATATTTTCGGCCGCACCGGCGGCGAGGAATTCACCGCCGTGATCGTCGAGGCCAGCGGATCGGAGGC
CCTGGAGGTCGCCCAACGGCTTTGCGCCGTGGTGGCCCAAGGCAAGATTGTTGGCCCCGGGGGGGAGGCGATCGGCGTCA
CCCTGTCGATCGGCGTCAGTCTGTTGCGCGGGCGCGACATGGCCTTCGAGATCCTCTCAAGGGAGGCCGACCAAGCCATG
TATGGCGCCAAACGGGCGGGACGCAACCGGGTCGTGGTTCATCGGGACGCCTGGGGCGCGCTCGGCTAG

Upstream 100 bases:

>100_bases
GTCTTTGCGAGGCGGATCCCATGGACCGATCGTCCCGGTTTGGCGCGGCCACAGGCCGGCGCGCACCCTTATCCCACCGT
CCCTTCTAAGCCGGGTCGCG

Downstream 100 bases:

>100_bases
GCCTTGATGGGGTGTTTGTCCTGTCCCGGCGATCGGGAATGGAATGGATCTAAGATCTGGGGTATCGTGGAGCGTGTGCG
GGGAGGGATGTCTCCGAGGA

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MLALTRLLLLVLVVACATPAFAEERGADHILARAFLEDPAGVLTIDEVARGDFTPFGPNFSKGYTQSVYWVRLLVRASAA
PEKTVLFIRPSFLNEVRLFYRDRASPDGWATRVSGNRYPFSERDRKSVALGFVVDVTHPQEEFYLRIKTNTQMQIDVQAV
SPDVAADTDSRRDMVMVFFVTSMILMIFWGVNEYSFDRYRIFALFCIHQLAFTLFGISAVGYFSPFWVMIFPGIVDRAFY
FLYMSISFTLILFCRELFKPYQPHPVLAKGLDLARWAFPLQIIALIAGYDALAVISNALLIKGALFYFVFVAFSLKVDLD
PTRRTLRIFFIGIALINLIFWMTPRVGGELKWLGLSAVQALVIDGFVMGILFAWLAHGRGRQVREDAQRKIRLVKESYLA
ERDLKARAEREARVDYLTGVLNRRSFFELAEGELARSIRFGRPLAVLMIDIDYFKAVNDTWGHGVGDEVLQNVAGLIGQT
LRGADIFGRTGGEEFTAVIVEASGSEALEVAQRLCAVVAQGKIVGPGGEAIGVTLSIGVSLLRGRDMAFEILSREADQAM
YGAKRAGRNRVVVHRDAWGALG

Sequences:

>Translated_582_residues
MLALTRLLLLVLVVACATPAFAEERGADHILARAFLEDPAGVLTIDEVARGDFTPFGPNFSKGYTQSVYWVRLLVRASAA
PEKTVLFIRPSFLNEVRLFYRDRASPDGWATRVSGNRYPFSERDRKSVALGFVVDVTHPQEEFYLRIKTNTQMQIDVQAV
SPDVAADTDSRRDMVMVFFVTSMILMIFWGVNEYSFDRYRIFALFCIHQLAFTLFGISAVGYFSPFWVMIFPGIVDRAFY
FLYMSISFTLILFCRELFKPYQPHPVLAKGLDLARWAFPLQIIALIAGYDALAVISNALLIKGALFYFVFVAFSLKVDLD
PTRRTLRIFFIGIALINLIFWMTPRVGGELKWLGLSAVQALVIDGFVMGILFAWLAHGRGRQVREDAQRKIRLVKESYLA
ERDLKARAEREARVDYLTGVLNRRSFFELAEGELARSIRFGRPLAVLMIDIDYFKAVNDTWGHGVGDEVLQNVAGLIGQT
LRGADIFGRTGGEEFTAVIVEASGSEALEVAQRLCAVVAQGKIVGPGGEAIGVTLSIGVSLLRGRDMAFEILSREADQAM
YGAKRAGRNRVVVHRDAWGALG
>Mature_582_residues
MLALTRLLLLVLVVACATPAFAEERGADHILARAFLEDPAGVLTIDEVARGDFTPFGPNFSKGYTQSVYWVRLLVRASAA
PEKTVLFIRPSFLNEVRLFYRDRASPDGWATRVSGNRYPFSERDRKSVALGFVVDVTHPQEEFYLRIKTNTQMQIDVQAV
SPDVAADTDSRRDMVMVFFVTSMILMIFWGVNEYSFDRYRIFALFCIHQLAFTLFGISAVGYFSPFWVMIFPGIVDRAFY
FLYMSISFTLILFCRELFKPYQPHPVLAKGLDLARWAFPLQIIALIAGYDALAVISNALLIKGALFYFVFVAFSLKVDLD
PTRRTLRIFFIGIALINLIFWMTPRVGGELKWLGLSAVQALVIDGFVMGILFAWLAHGRGRQVREDAQRKIRLVKESYLA
ERDLKARAEREARVDYLTGVLNRRSFFELAEGELARSIRFGRPLAVLMIDIDYFKAVNDTWGHGVGDEVLQNVAGLIGQT
LRGADIFGRTGGEEFTAVIVEASGSEALEVAQRLCAVVAQGKIVGPGGEAIGVTLSIGVSLLRGRDMAFEILSREADQAM
YGAKRAGRNRVVVHRDAWGALG

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=190, Percent_Identity=40, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87082007, Length=187, Percent_Identity=37.9679144385027, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1787262, Length=168, Percent_Identity=39.2857142857143, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=35.0282485875706, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=35.4651162790698, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788085, Length=171, Percent_Identity=32.7485380116959, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1787816, Length=163, Percent_Identity=34.9693251533742, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1787802, Length=190, Percent_Identity=30.5263157894737, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788381, Length=189, Percent_Identity=31.7460317460317, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1787541, Length=173, Percent_Identity=30.0578034682081, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=167, Percent_Identity=26.3473053892216, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788956, Length=175, Percent_Identity=31.4285714285714, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI87081977, Length=161, Percent_Identity=27.9503105590062, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 65119; Mature: 65119

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLALTRLLLLVLVVACATPAFAEERGADHILARAFLEDPAGVLTIDEVARGDFTPFGPNF
CHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHCCCCCEEEHHHHHCCCCCCCCCCC
SKGYTQSVYWVRLLVRASAAPEKTVLFIRPSFLNEVRLFYRDRASPDGWATRVSGNRYPF
CCCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCEEECCCCCCCC
SERDRKSVALGFVVDVTHPQEEFYLRIKTNTQMQIDVQAVSPDVAADTDSRRDMVMVFFV
CCCCCCEEEEEEEEECCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCCEEHHHHH
TSMILMIFWGVNEYSFDRYRIFALFCIHQLAFTLFGISAVGYFSPFWVMIFPGIVDRAFY
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
FLYMSISFTLILFCRELFKPYQPHPVLAKGLDLARWAFPLQIIALIAGYDALAVISNALL
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
IKGALFYFVFVAFSLKVDLDPTRRTLRIFFIGIALINLIFWMTPRVGGELKWLGLSAVQA
HHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHH
LVIDGFVMGILFAWLAHGRGRQVREDAQRKIRLVKESYLAERDLKARAEREARVDYLTGV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNRRSFFELAEGELARSIRFGRPLAVLMIDIDYFKAVNDTWGHGVGDEVLQNVAGLIGQT
HCCHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LRGADIFGRTGGEEFTAVIVEASGSEALEVAQRLCAVVAQGKIVGPGGEAIGVTLSIGVS
HCCCHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCEEECCCCCEEEEEHHHHHH
LLRGRDMAFEILSREADQAMYGAKRAGRNRVVVHRDAWGALG
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MLALTRLLLLVLVVACATPAFAEERGADHILARAFLEDPAGVLTIDEVARGDFTPFGPNF
CHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHCCCCCEEEHHHHHCCCCCCCCCCC
SKGYTQSVYWVRLLVRASAAPEKTVLFIRPSFLNEVRLFYRDRASPDGWATRVSGNRYPF
CCCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCEEECCCCCCCC
SERDRKSVALGFVVDVTHPQEEFYLRIKTNTQMQIDVQAVSPDVAADTDSRRDMVMVFFV
CCCCCCEEEEEEEEECCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCCEEHHHHH
TSMILMIFWGVNEYSFDRYRIFALFCIHQLAFTLFGISAVGYFSPFWVMIFPGIVDRAFY
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
FLYMSISFTLILFCRELFKPYQPHPVLAKGLDLARWAFPLQIIALIAGYDALAVISNALL
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
IKGALFYFVFVAFSLKVDLDPTRRTLRIFFIGIALINLIFWMTPRVGGELKWLGLSAVQA
HHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHH
LVIDGFVMGILFAWLAHGRGRQVREDAQRKIRLVKESYLAERDLKARAEREARVDYLTGV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNRRSFFELAEGELARSIRFGRPLAVLMIDIDYFKAVNDTWGHGVGDEVLQNVAGLIGQT
HCCHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LRGADIFGRTGGEEFTAVIVEASGSEALEVAQRLCAVVAQGKIVGPGGEAIGVTLSIGVS
HCCCHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCEEECCCCCEEEEEHHHHHH
LLRGRDMAFEILSREADQAMYGAKRAGRNRVVVHRDAWGALG
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]