Click here to switch to the map view.

The map label for this gene is yhfT [H]

Identifier: 83593852

GI number: 83593852

Start: 2927279

End: 2928658

Strand: Reverse

Name: yhfT [H]

Synonym: Rru_A2517

Alternate gene names: 83593852

Gene position: 2928658-2927279 (Counterclockwise)

Preceding gene: 83593854

Following gene: 83593849

Centisome position: 67.28

GC content: 70.8

Gene sequence:

>1380_bases
ATGACAAGCCCTAGCAAGACCCCCAATCTGTTCGCCCCCCTTGCCGAGATCGACGCCGAACGACCGGCGCTGATCAGTGA
AGAGGGACTCCTGACCCATGGCGCCCTGCGGCGCCGGGTGGGCGCCGTCCAGCGTTGGCTGGCCGATCAACCCGCCGAAC
GGCCGTTGCTGCTGAGCGCCACCGCCGCGTCCGACCTGCTGCCCGCCTTCATCGCCGCCGGCGCCCTGGGGCGAACGGCG
GCGGTGGGCGATCGCCGGTGGTCCGAGGCGGTGCGGGCGCGGGTGGTCGCCGCCCTTGGTCAGCCGTTGATCGGCGAGTC
CCTTCCGGCTTTCCCAACCGGAGAGGCGGCCGACGACCCGCCGCGCTTCGCGCCCAATCCCTCCCGGCCCTTCTATGTCG
GCTTCACCTCGGGCACCACCGGCCTGCCCAAGGCCTATGCGCGCAGCCATCGCTCGTGGATCGAAACGATGGTGGCCGGG
CGCGATGGCTTCGCCCTGGACGCCGCGGATCGCATTCTGGTGTTGGGGCCGCTCGTCCATTCCCTGGCGCTCTATGGCGC
GCTTCAGGCCCTTTGGCTGGGCGGGGCGGCGATCGTCGACGGGATGGGGGGATGGGGCGTCGATCAGGGGGCGACGGTGG
TGATCGGGGTGCCGCCGCTGCTGGCCGCCCTGGCCCGCACCGGCCCCCATTCGGCGATCCGCACGGTTCTTTCGTCGGGC
CAAGCCCTTGATCCCACCACCGAGGCGGTGCTGCGTCGCGGCTTTCCCAAGGCCGTCCTCGTTGATTTCTATGGAACCTC
CGAACAGAGCTTCATCGCTTGGCGCGCCGTGGGCGAGGAGGCGACCCGGGGCACGGTCGGCCGCCCCTTCGCCGGGGTCG
AGGCCGAAATCCGCGATGCCGAGGGTCGGCCCGTCGCCCCAGGGGTCCAGGGCCGCTTGCATGTGAAAAGCCCGATGGTC
TTCGACGGCTATCTTGAAGGCCTTGATCGGGGAGGCTTTTCCAGCAAGGACGGCTGGTCGAGCGTTGGCGATCTCGGCTG
GCTGGGCGAGGATGGAACCCTGACCCTGGCCGGGCGCGAGGGCGGCATGGTGGTGGTTCGCGGGGTCAATGTCTTTCCCC
AGGAGGTCGAGGCGGCGCTTAAGGCCCTGCCCGGGATCCTCGATGCCGGGGTCGTCGGTCTGCCCGACGCCGGACGCGGC
GCCTTGCTGGTCGCCCTGGTGGAGGGCGAAGGCGTGGTCGATCCCGCCGCCCTGGGGGCCTTGCGCCCGGAGAAACGGCC
GCGGCGGATTATCGGCGTTAGCGCCCTGCCTCGCACCCTGACGGGGAAAATCGACCGCCGGGCCCTGGCCGAGCTGGCGG
CCGGCTTGATCGCCCGATAA

Upstream 100 bases:

>100_bases
CCGAAGCGGGAGTGACGCTCTCCCCGCAAGTGGGGGGAGGGGAGGGCCGAGGCAAGCCGGGGGTTGAAAGCCTGCCAGTG
CATGGCCGATAAAGAGCCCC

Downstream 100 bases:

>100_bases
GGCGGTCAGGCCGTCGCCGCTCCGCTGTCCTTGATCGGCTGGGGAGACGGGACGGCTTGAACGCCCCGGGGGGCCTTGAT
CGGGAAAACCACGGTGAAGG

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MTSPSKTPNLFAPLAEIDAERPALISEEGLLTHGALRRRVGAVQRWLADQPAERPLLLSATAASDLLPAFIAAGALGRTA
AVGDRRWSEAVRARVVAALGQPLIGESLPAFPTGEAADDPPRFAPNPSRPFYVGFTSGTTGLPKAYARSHRSWIETMVAG
RDGFALDAADRILVLGPLVHSLALYGALQALWLGGAAIVDGMGGWGVDQGATVVIGVPPLLAALARTGPHSAIRTVLSSG
QALDPTTEAVLRRGFPKAVLVDFYGTSEQSFIAWRAVGEEATRGTVGRPFAGVEAEIRDAEGRPVAPGVQGRLHVKSPMV
FDGYLEGLDRGGFSSKDGWSSVGDLGWLGEDGTLTLAGREGGMVVVRGVNVFPQEVEAALKALPGILDAGVVGLPDAGRG
ALLVALVEGEGVVDPAALGALRPEKRPRRIIGVSALPRTLTGKIDRRALAELAAGLIAR

Sequences:

>Translated_459_residues
MTSPSKTPNLFAPLAEIDAERPALISEEGLLTHGALRRRVGAVQRWLADQPAERPLLLSATAASDLLPAFIAAGALGRTA
AVGDRRWSEAVRARVVAALGQPLIGESLPAFPTGEAADDPPRFAPNPSRPFYVGFTSGTTGLPKAYARSHRSWIETMVAG
RDGFALDAADRILVLGPLVHSLALYGALQALWLGGAAIVDGMGGWGVDQGATVVIGVPPLLAALARTGPHSAIRTVLSSG
QALDPTTEAVLRRGFPKAVLVDFYGTSEQSFIAWRAVGEEATRGTVGRPFAGVEAEIRDAEGRPVAPGVQGRLHVKSPMV
FDGYLEGLDRGGFSSKDGWSSVGDLGWLGEDGTLTLAGREGGMVVVRGVNVFPQEVEAALKALPGILDAGVVGLPDAGRG
ALLVALVEGEGVVDPAALGALRPEKRPRRIIGVSALPRTLTGKIDRRALAELAAGLIAR
>Mature_458_residues
TSPSKTPNLFAPLAEIDAERPALISEEGLLTHGALRRRVGAVQRWLADQPAERPLLLSATAASDLLPAFIAAGALGRTAA
VGDRRWSEAVRARVVAALGQPLIGESLPAFPTGEAADDPPRFAPNPSRPFYVGFTSGTTGLPKAYARSHRSWIETMVAGR
DGFALDAADRILVLGPLVHSLALYGALQALWLGGAAIVDGMGGWGVDQGATVVIGVPPLLAALARTGPHSAIRTVLSSGQ
ALDPTTEAVLRRGFPKAVLVDFYGTSEQSFIAWRAVGEEATRGTVGRPFAGVEAEIRDAEGRPVAPGVQGRLHVKSPMVF
DGYLEGLDRGGFSSKDGWSSVGDLGWLGEDGTLTLAGREGGMVVVRGVNVFPQEVEAALKALPGILDAGVVGLPDAGRGA
LLVALVEGEGVVDPAALGALRPEKRPRRIIGVSALPRTLTGKIDRRALAELAAGLIAR

Specific function: May be involved in fatty acid metabolism [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=358, Percent_Identity=26.2569832402235, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI122937307, Length=357, Percent_Identity=26.6106442577031, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI157311624, Length=353, Percent_Identity=26.9121813031161, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI157311622, Length=353, Percent_Identity=26.9121813031161, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI156151445, Length=352, Percent_Identity=25.5681818181818, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI42544132, Length=352, Percent_Identity=22.7272727272727, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI187761345, Length=422, Percent_Identity=28.9099526066351, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI187761343, Length=422, Percent_Identity=28.9099526066351, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI115511026, Length=365, Percent_Identity=24.6575342465753, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI58082049, Length=353, Percent_Identity=26.0623229461756, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788107, Length=349, Percent_Identity=27.2206303724928, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI145693145, Length=342, Percent_Identity=28.3625730994152, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790505, Length=387, Percent_Identity=26.3565891472868, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786810, Length=350, Percent_Identity=24.8571428571429, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789201, Length=312, Percent_Identity=26.6025641025641, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786801, Length=387, Percent_Identity=26.0981912144703, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI221142682, Length=339, Percent_Identity=27.4336283185841, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=339, Percent_Identity=26.8436578171091, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI32563687, Length=355, Percent_Identity=27.6056338028169, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=377, Percent_Identity=26.790450928382, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=361, Percent_Identity=25.4847645429363, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71994694, Length=361, Percent_Identity=25.4847645429363, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17560308, Length=364, Percent_Identity=26.3736263736264, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71994703, Length=361, Percent_Identity=25.4847645429363, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17538037, Length=390, Percent_Identity=26.9230769230769, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17559526, Length=429, Percent_Identity=23.7762237762238, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25141359, Length=338, Percent_Identity=25.4437869822485, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17531443, Length=350, Percent_Identity=22.2857142857143, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=340, Percent_Identity=27.6470588235294, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24581924, Length=354, Percent_Identity=27.9661016949153, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI18859661, Length=352, Percent_Identity=27.5568181818182, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20130357, Length=339, Percent_Identity=24.7787610619469, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355181, Length=338, Percent_Identity=24.5562130177515, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21356947, Length=353, Percent_Identity=23.2294617563739, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355225, Length=377, Percent_Identity=23.342175066313, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 47731; Mature: 47600

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPSKTPNLFAPLAEIDAERPALISEEGLLTHGALRRRVGAVQRWLADQPAERPLLLSA
CCCCCCCCCHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
TAASDLLPAFIAAGALGRTAAVGDRRWSEAVRARVVAALGQPLIGESLPAFPTGEAADDP
CHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCC
PRFAPNPSRPFYVGFTSGTTGLPKAYARSHRSWIETMVAGRDGFALDAADRILVLGPLVH
CCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCEEEHHHHHH
SLALYGALQALWLGGAAIVDGMGGWGVDQGATVVIGVPPLLAALARTGPHSAIRTVLSSG
HHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHCCCCHHHHHHHHHCC
QALDPTTEAVLRRGFPKAVLVDFYGTSEQSFIAWRAVGEEATRGTVGRPFAGVEAEIRDA
CCCCHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCHHHCCCCCCCCCCCCCEEECC
EGRPVAPGVQGRLHVKSPMVFDGYLEGLDRGGFSSKDGWSSVGDLGWLGEDGTLTLAGRE
CCCCCCCCCCCEEEECCCCEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
GGMVVVRGVNVFPQEVEAALKALPGILDAGVVGLPDAGRGALLVALVEGEGVVDPAALGA
CCEEEEECCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHHCC
LRPEKRPRRIIGVSALPRTLTGKIDRRALAELAAGLIAR
CCCCCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSPSKTPNLFAPLAEIDAERPALISEEGLLTHGALRRRVGAVQRWLADQPAERPLLLSA
CCCCCCCCHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
TAASDLLPAFIAAGALGRTAAVGDRRWSEAVRARVVAALGQPLIGESLPAFPTGEAADDP
CHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCC
PRFAPNPSRPFYVGFTSGTTGLPKAYARSHRSWIETMVAGRDGFALDAADRILVLGPLVH
CCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCEEEHHHHHH
SLALYGALQALWLGGAAIVDGMGGWGVDQGATVVIGVPPLLAALARTGPHSAIRTVLSSG
HHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHCCCCHHHHHHHHHCC
QALDPTTEAVLRRGFPKAVLVDFYGTSEQSFIAWRAVGEEATRGTVGRPFAGVEAEIRDA
CCCCHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCHHHCCCCCCCCCCCCCEEECC
EGRPVAPGVQGRLHVKSPMVFDGYLEGLDRGGFSSKDGWSSVGDLGWLGEDGTLTLAGRE
CCCCCCCCCCCEEEECCCCEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
GGMVVVRGVNVFPQEVEAALKALPGILDAGVVGLPDAGRGALLVALVEGEGVVDPAALGA
CCEEEEECCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHHCC
LRPEKRPRRIIGVSALPRTLTGKIDRRALAELAAGLIAR
CCCCCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]