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The map label for this gene is prfC [H]

Identifier: 83593832

GI number: 83593832

Start: 2903546

End: 2905138

Strand: Reverse

Name: prfC [H]

Synonym: Rru_A2497

Alternate gene names: 83593832

Gene position: 2905138-2903546 (Counterclockwise)

Preceding gene: 83593838

Following gene: 83593831

Centisome position: 66.74

GC content: 63.28

Gene sequence:

>1593_bases
ATGACCGCGATCGAAGATGAAGTCCAGCGCCGGCGTACCTTCGCCATCATTTCCCACCCGGACGCGGGCAAAACCACGCT
GACCGAGAAGCTTTTGCTGTTCGGGGGAGCCATCCAGCTCGCCGGTGCGGTGAAGGCGCGGGGCGAGGTGCGCCGCGCGC
GTTCGGACTGGATGAAGGTGGAACAGGAGCGCGGCATTTCCGTTGCCTCCTCGGTGATGAGCTACGAATACGCCGGCCGC
GCCTTCAATCTGCTCGACACCCCCGGCCACGAGGACTTCAGCGAAGATACCTACCGCACCCTGACGGCCGTGGACAGCGC
GGTGATGGTGATCGACGCCGCCAAGGGCATCGAAGAGCAAACCCGCAAGCTGTTCGAAGTCTGTCGTCTGCGCGACGTGC
CGATCATCACCTTTATCAACAAGCTCGACCGCGAAGGCCAAGATCCCTTCGATTTGCTCGACGAGATCGAACAGACCTTG
GCGCTGGACGTGACGCCGGCCAGTTGGCCGATCGGCATGGGCCGTGACTTCCTGGGCTGCTACGACCTGTTCAACGATCG
TTTGGCGCTGATGGCCAAGGGGGCCAAGGGAAGCCTGCCGACGCCAGGGGAACGCTGTGAAGGTCTGGACGATCCCAAGC
TTGACAAGATGCTGCCCGCCGATGCGGTGGCCAAGCTGCGCGGCGATGTCGAGATGGTGCGCGGTCTCTGTCCGGCTTTC
GATGCCGAGGCCTATCGCGCCGGCACGATGACCCCGGTCTTCTTCGGCAGCGCCGTCAATAATTTCGGCGTGCGCGAACT
GCTCGAAGGCGTGGCCCATCTGGCGCCGTCGCCGCGCCCACAACCGACCACCACCCGCGACATCCAGCCGACGGAAGACA
AGGTCACCGGCTTCGTTTTCAAGATCCAGGCCAATATGGATCCCAAGCACCGCGACCGCATCGCCTTCGTGCGCCTGTGC
TCGGGTCCGTTCCGTCGCGGCATGAAGCTGTTCCATGTGCGCACCGGCAAACAGATGAACATGCATAATCCGGTGATGTT
CCTGGCCCGCGAGCGCGAACTGGCCGAAGAGGCCTTCGCCGGCGATATCATGGGCGTGCCCAACCACGGCCAGCTGCGCA
TCGGCGATACCCTGAGCGAGGGCGAGAACCTGCGGGTCACCGGCATTCCCAGCTTCGCGCCGGAAATGCTTCAGAAGGTG
CGGCCCAAGGACCCCTTGCGCGCCAAACATCTGGGGCGGGCCTTGCAGCAGATCGCCGAAGAAGGCGCCGCGCGCGTCTT
CAAGCCGATCATGGGCGCCGATTGGATCGTTGGCGTGGTTGGTCCCTTGCAGTTCGAGGTTCTGGCCGATCGCATCCGCA
CCGAATTCGATGTGCCGGTTTTGTTCGAATCGACAGCGCTGTACACGGCGCGCTGGGTGACGGCCGAGGATCCTTTGGTT
CTCAAGAAGTTCCTCGATGGCAATCAGGCTTCGCTGGCCGAGGATCATGACGGCGATCCGGTCTTCCTGGCCCGCAACGC
CTGGCACCTGGACCGCGCCGCCGAGGATTTCCCGCTGATCGTCTTCCATAAGACCAAAGAACAGGCGGTGTGA

Upstream 100 bases:

>100_bases
GACGGGGGGGCGGAAAAAAACCTCTGGCTTTCGGCGGCCGGGGGAAGCAGAGTCCGGCCCCTTGTCCAGCCCCTCTTTCC
TGTCGTCAGACCACGGATCC

Downstream 100 bases:

>100_bases
CGGGGCGGGGGCCCGCCGGTCCCCGCCGCGTTCCTTCCCTTTTTCGGATCCGCAGCCGATGTCCGGTCTTGTCGACAGGC
GCTTCAAAAGAAACGTGCTC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTAIEDEVQRRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGAVKARGEVRRARSDWMKVEQERGISVASSVMSYEYAGR
AFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEEQTRKLFEVCRLRDVPIITFINKLDREGQDPFDLLDEIEQTL
ALDVTPASWPIGMGRDFLGCYDLFNDRLALMAKGAKGSLPTPGERCEGLDDPKLDKMLPADAVAKLRGDVEMVRGLCPAF
DAEAYRAGTMTPVFFGSAVNNFGVRELLEGVAHLAPSPRPQPTTTRDIQPTEDKVTGFVFKIQANMDPKHRDRIAFVRLC
SGPFRRGMKLFHVRTGKQMNMHNPVMFLARERELAEEAFAGDIMGVPNHGQLRIGDTLSEGENLRVTGIPSFAPEMLQKV
RPKDPLRAKHLGRALQQIAEEGAARVFKPIMGADWIVGVVGPLQFEVLADRIRTEFDVPVLFESTALYTARWVTAEDPLV
LKKFLDGNQASLAEDHDGDPVFLARNAWHLDRAAEDFPLIVFHKTKEQAV

Sequences:

>Translated_530_residues
MTAIEDEVQRRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGAVKARGEVRRARSDWMKVEQERGISVASSVMSYEYAGR
AFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEEQTRKLFEVCRLRDVPIITFINKLDREGQDPFDLLDEIEQTL
ALDVTPASWPIGMGRDFLGCYDLFNDRLALMAKGAKGSLPTPGERCEGLDDPKLDKMLPADAVAKLRGDVEMVRGLCPAF
DAEAYRAGTMTPVFFGSAVNNFGVRELLEGVAHLAPSPRPQPTTTRDIQPTEDKVTGFVFKIQANMDPKHRDRIAFVRLC
SGPFRRGMKLFHVRTGKQMNMHNPVMFLARERELAEEAFAGDIMGVPNHGQLRIGDTLSEGENLRVTGIPSFAPEMLQKV
RPKDPLRAKHLGRALQQIAEEGAARVFKPIMGADWIVGVVGPLQFEVLADRIRTEFDVPVLFESTALYTARWVTAEDPLV
LKKFLDGNQASLAEDHDGDPVFLARNAWHLDRAAEDFPLIVFHKTKEQAV
>Mature_529_residues
TAIEDEVQRRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGAVKARGEVRRARSDWMKVEQERGISVASSVMSYEYAGRA
FNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEEQTRKLFEVCRLRDVPIITFINKLDREGQDPFDLLDEIEQTLA
LDVTPASWPIGMGRDFLGCYDLFNDRLALMAKGAKGSLPTPGERCEGLDDPKLDKMLPADAVAKLRGDVEMVRGLCPAFD
AEAYRAGTMTPVFFGSAVNNFGVRELLEGVAHLAPSPRPQPTTTRDIQPTEDKVTGFVFKIQANMDPKHRDRIAFVRLCS
GPFRRGMKLFHVRTGKQMNMHNPVMFLARERELAEEAFAGDIMGVPNHGQLRIGDTLSEGENLRVTGIPSFAPEMLQKVR
PKDPLRAKHLGRALQQIAEEGAARVFKPIMGADWIVGVVGPLQFEVLADRIRTEFDVPVLFESTALYTARWVTAEDPLVL
KKFLDGNQASLAEDHDGDPVFLARNAWHLDRAAEDFPLIVFHKTKEQAV

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=509, Percent_Identity=25.9332023575639, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI19923640, Length=501, Percent_Identity=24.750499001996, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI25306283, Length=351, Percent_Identity=27.6353276353276, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI25306287, Length=476, Percent_Identity=25.4201680672269, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI157426893, Length=157, Percent_Identity=35.031847133758, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI94966754, Length=150, Percent_Identity=32.6666666666667, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI4503483, Length=158, Percent_Identity=33.5443037974684, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI310132016, Length=129, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI310110807, Length=129, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI310123363, Length=129, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1790835, Length=522, Percent_Identity=51.9157088122605, Blast_Score=566, Evalue=1e-162,
Organism=Escherichia coli, GI1789738, Length=486, Percent_Identity=27.3662551440329, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1788922, Length=171, Percent_Identity=33.9181286549708, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=34.8993288590604, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=493, Percent_Identity=27.5862068965517, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17556745, Length=481, Percent_Identity=23.9085239085239, Blast_Score=115, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17557151, Length=137, Percent_Identity=35.036496350365, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=34.5864661654135, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=34.5864661654135, Blast_Score=69, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=481, Percent_Identity=29.3139293139293, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6322359, Length=560, Percent_Identity=23.3928571428571, Blast_Score=123, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6324707, Length=160, Percent_Identity=33.125, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320593, Length=160, Percent_Identity=33.125, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=176, Percent_Identity=30.1136363636364, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24582462, Length=491, Percent_Identity=26.2729124236253, Blast_Score=163, Evalue=3e-40,
Organism=Drosophila melanogaster, GI221458488, Length=391, Percent_Identity=28.9002557544757, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI78706572, Length=174, Percent_Identity=33.9080459770115, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585711, Length=159, Percent_Identity=32.7044025157233, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24585713, Length=159, Percent_Identity=32.7044025157233, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24585709, Length=159, Percent_Identity=32.7044025157233, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI28574573, Length=165, Percent_Identity=35.1515151515151, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 58943; Mature: 58812

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIEDEVQRRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGAVKARGEVRRARSDWMKV
CCCCHHHHHHHHEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
EQERGISVASSVMSYEYAGRAFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEEQ
HHHCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCHHHH
TRKLFEVCRLRDVPIITFINKLDREGQDPFDLLDEIEQTLALDVTPASWPIGMGRDFLGC
HHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHH
YDLFNDRLALMAKGAKGSLPTPGERCEGLDDPKLDKMLPADAVAKLRGDVEMVRGLCPAF
HHHHCCCEEEEECCCCCCCCCCHHHCCCCCCCCHHHCCCHHHHHHHHCCHHHHHHCCCCC
DAEAYRAGTMTPVFFGSAVNNFGVRELLEGVAHLAPSPRPQPTTTRDIQPTEDKVTGFVF
CCHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCEEEE
KIQANMDPKHRDRIAFVRLCSGPFRRGMKLFHVRTGKQMNMHNPVMFLARERELAEEAFA
EEEECCCCCHHHHHHHHHHHCCHHHCCCEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHC
GDIMGVPNHGQLRIGDTLSEGENLRVTGIPSFAPEMLQKVRPKDPLRAKHLGRALQQIAE
CCCCCCCCCCCEEECCCCCCCCCEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
EGAARVFKPIMGADWIVGVVGPLQFEVLADRIRTEFDVPVLFESTALYTARWVTAEDPLV
HHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHCCCCCCEEEECCEEEEEEEECCCCCHH
LKKFLDGNQASLAEDHDGDPVFLARNAWHLDRAAEDFPLIVFHKTKEQAV
HHHHCCCCCCCCCCCCCCCEEEEECCCHHHHCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
TAIEDEVQRRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGAVKARGEVRRARSDWMKV
CCCHHHHHHHHEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
EQERGISVASSVMSYEYAGRAFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEEQ
HHHCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCHHHH
TRKLFEVCRLRDVPIITFINKLDREGQDPFDLLDEIEQTLALDVTPASWPIGMGRDFLGC
HHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHH
YDLFNDRLALMAKGAKGSLPTPGERCEGLDDPKLDKMLPADAVAKLRGDVEMVRGLCPAF
HHHHCCCEEEEECCCCCCCCCCHHHCCCCCCCCHHHCCCHHHHHHHHCCHHHHHHCCCCC
DAEAYRAGTMTPVFFGSAVNNFGVRELLEGVAHLAPSPRPQPTTTRDIQPTEDKVTGFVF
CCHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCEEEE
KIQANMDPKHRDRIAFVRLCSGPFRRGMKLFHVRTGKQMNMHNPVMFLARERELAEEAFA
EEEECCCCCHHHHHHHHHHHCCHHHCCCEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHC
GDIMGVPNHGQLRIGDTLSEGENLRVTGIPSFAPEMLQKVRPKDPLRAKHLGRALQQIAE
CCCCCCCCCCCEEECCCCCCCCCEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
EGAARVFKPIMGADWIVGVVGPLQFEVLADRIRTEFDVPVLFESTALYTARWVTAEDPLV
HHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHCCCCCCEEEECCEEEEEEEECCCCCHH
LKKFLDGNQASLAEDHDGDPVFLARNAWHLDRAAEDFPLIVFHKTKEQAV
HHHHCCCCCCCCCCCCCCCEEEEECCCHHHHCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA