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The map label for this gene is nifV [H]

Identifier: 83593604

GI number: 83593604

Start: 2629099

End: 2630277

Strand: Reverse

Name: nifV [H]

Synonym: Rru_A2269

Alternate gene names: 83593604

Gene position: 2630277-2629099 (Counterclockwise)

Preceding gene: 83593605

Following gene: 83593603

Centisome position: 60.43

GC content: 68.19

Gene sequence:

>1179_bases
ATGGATTGGTCATCGGTCATCGTCAACGATACCACGCTGCGCGACGGCGAGCAGACCGCCGGGGTGATCTTCACCCTGGG
CGAAAAGGTGGCGATCGCCAAGGCCCTGGAGCGCGCCGGCGTGCCCGAGATGGAAGTCGGCATCCCGGCCATGGGGCCCG
AGGAGCAGGAGTCGATCCGCGCCGTCGTCGCCCAATGCGAGGCCAGCCGGCCGATCGCCTGGTGCCGCATGCATATGACC
GACCTTGACGCGGCGGCGGCCTGCGGCGTCGATATGGTCAATCTGTCGATCCCGGTTTCCGACCAGCAGATCAAGGGCAA
GCTGGGCCGCGATCGCGGCTGGGTGCTCGCCCATCTGGAGACCATGATCAAGACCGCCCTGGAAAAGGGGCTGGAGGTCG
CCCTGGGCGGCGAGGATTCCAGCCGCGCCGATCCCGAGTTCCTGCTTCGGGTGATCGAACGCGCCGAAGCCAGCGGCGCC
CGGCGCTTCCGCTTCGCCGATACCCTGGGGATCATGGATCCCTTCACCACCTTGCAGATGTTCAAGCGCCTGCGCGCCGC
CAGCGGCCTGGAACTGGAAATCCATGCCCACGATGATCTGGGGCTGGCGACGGCGAATTCCCTGGGCGCCGTGCTTGGCG
GGGCCACCCATGTTTCGACCACCGTCAATGGCCTGGGCGAGCGGGCCGGCAACGCCCCCCTTGAAGAGGTGGTGATGGCC
TGCCGCAATCTGTATGGCCGCCATACCGGCATCGCGCCGATGGCCCTGGCGGCGGTCTCCAAACTGGTGGCCGAGGCCTC
GGGGCGGCCGGTTCCGGCGAACAAGCCGATCGTCGGCGAGGCGATCTTCACCCATGAATCGGGGATCCATGTCAGCGGCC
TGCTGCGCGATCCCAAGAATTACGAGGCGTTGTCCCCCGCCGATCTCGGGCGTTGCCATCGTCTGGTTCTGGGCAAGCAT
TCGGGCGTCGCCAGCATCGTTCACGCCTGCAACGCCCTGGGGCTGGCCCCCGGAGAGGCCCAGGCGCGGGCCATGCTGGC
GCGCGTCCGCCTCCATGCCGGGGCGACCAAGCGCCCGCCGACGGACGCCGATCTGCGCCGCTTCTTCGACGAAACCCGCT
CGGTGGCCGAGGCCATCGAAACCCTTGATTTCAGCCGCCCGCCCCAGGCGGCGAGCTGA

Upstream 100 bases:

>100_bases
CCGCCCATGCCGTGCCCTGGGCCGGTCAGCCCCCGGTCAAAGCCGCCGCTGCCGCCACCGCGGCCTTGGCCTGAGGCGGA
CGGGCGGGGAGGATCGGAAG

Downstream 100 bases:

>100_bases
CCCGCGCAAAGGGACGCCCCGTGATGAGCCGCTTTCTCGAAGATCTGCGGAGTCTGTCGAGCGCCGAGGAGTTTTTCAGC
TTCCTCGGAGTCGAATTTAA

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MDWSSVIVNDTTLRDGEQTAGVIFTLGEKVAIAKALERAGVPEMEVGIPAMGPEEQESIRAVVAQCEASRPIAWCRMHMT
DLDAAAACGVDMVNLSIPVSDQQIKGKLGRDRGWVLAHLETMIKTALEKGLEVALGGEDSSRADPEFLLRVIERAEASGA
RRFRFADTLGIMDPFTTLQMFKRLRAASGLELEIHAHDDLGLATANSLGAVLGGATHVSTTVNGLGERAGNAPLEEVVMA
CRNLYGRHTGIAPMALAAVSKLVAEASGRPVPANKPIVGEAIFTHESGIHVSGLLRDPKNYEALSPADLGRCHRLVLGKH
SGVASIVHACNALGLAPGEAQARAMLARVRLHAGATKRPPTDADLRRFFDETRSVAEAIETLDFSRPPQAAS

Sequences:

>Translated_392_residues
MDWSSVIVNDTTLRDGEQTAGVIFTLGEKVAIAKALERAGVPEMEVGIPAMGPEEQESIRAVVAQCEASRPIAWCRMHMT
DLDAAAACGVDMVNLSIPVSDQQIKGKLGRDRGWVLAHLETMIKTALEKGLEVALGGEDSSRADPEFLLRVIERAEASGA
RRFRFADTLGIMDPFTTLQMFKRLRAASGLELEIHAHDDLGLATANSLGAVLGGATHVSTTVNGLGERAGNAPLEEVVMA
CRNLYGRHTGIAPMALAAVSKLVAEASGRPVPANKPIVGEAIFTHESGIHVSGLLRDPKNYEALSPADLGRCHRLVLGKH
SGVASIVHACNALGLAPGEAQARAMLARVRLHAGATKRPPTDADLRRFFDETRSVAEAIETLDFSRPPQAAS
>Mature_392_residues
MDWSSVIVNDTTLRDGEQTAGVIFTLGEKVAIAKALERAGVPEMEVGIPAMGPEEQESIRAVVAQCEASRPIAWCRMHMT
DLDAAAACGVDMVNLSIPVSDQQIKGKLGRDRGWVLAHLETMIKTALEKGLEVALGGEDSSRADPEFLLRVIERAEASGA
RRFRFADTLGIMDPFTTLQMFKRLRAASGLELEIHAHDDLGLATANSLGAVLGGATHVSTTVNGLGERAGNAPLEEVVMA
CRNLYGRHTGIAPMALAAVSKLVAEASGRPVPANKPIVGEAIFTHESGIHVSGLLRDPKNYEALSPADLGRCHRLVLGKH
SGVASIVHACNALGLAPGEAQARAMLARVRLHAGATKRPPTDADLRRFFDETRSVAEAIETLDFSRPPQAAS

Specific function: This protein is a Fe-Mo-cofactor biosynthetic component [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=348, Percent_Identity=37.0689655172414, Blast_Score=200, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6320071, Length=362, Percent_Identity=29.8342541436464, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6320019, Length=362, Percent_Identity=29.5580110497238, Blast_Score=165, Evalue=1e-41,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.14 [H]

Molecular weight: Translated: 41692; Mature: 41692

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWSSVIVNDTTLRDGEQTAGVIFTLGEKVAIAKALERAGVPEMEVGIPAMGPEEQESIR
CCCCCEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHH
AVVAQCEASRPIAWCRMHMTDLDAAAACGVDMVNLSIPVSDQQIKGKLGRDRGWVLAHLE
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCCCHHHHHHH
TMIKTALEKGLEVALGGEDSSRADPEFLLRVIERAEASGARRFRFADTLGIMDPFTTLQM
HHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHCCCHHHHHHH
FKRLRAASGLELEIHAHDDLGLATANSLGAVLGGATHVSTTVNGLGERAGNAPLEEVVMA
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHH
CRNLYGRHTGIAPMALAAVSKLVAEASGRPVPANKPIVGEAIFTHESGIHVSGLLRDPKN
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCC
YEALSPADLGRCHRLVLGKHSGVASIVHACNALGLAPGEAQARAMLARVRLHAGATKRPP
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCC
TDADLRRFFDETRSVAEAIETLDFSRPPQAAS
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDWSSVIVNDTTLRDGEQTAGVIFTLGEKVAIAKALERAGVPEMEVGIPAMGPEEQESIR
CCCCCEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHH
AVVAQCEASRPIAWCRMHMTDLDAAAACGVDMVNLSIPVSDQQIKGKLGRDRGWVLAHLE
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCCCHHHHHHH
TMIKTALEKGLEVALGGEDSSRADPEFLLRVIERAEASGARRFRFADTLGIMDPFTTLQM
HHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHCCCHHHHHHH
FKRLRAASGLELEIHAHDDLGLATANSLGAVLGGATHVSTTVNGLGERAGNAPLEEVVMA
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHH
CRNLYGRHTGIAPMALAAVSKLVAEASGRPVPANKPIVGEAIFTHESGIHVSGLLRDPKN
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCC
YEALSPADLGRCHRLVLGKHSGVASIVHACNALGLAPGEAQARAMLARVRLHAGATKRPP
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCC
TDADLRRFFDETRSVAEAIETLDFSRPPQAAS
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644218; 3040672 [H]