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The map label for this gene is rne [H]

Identifier: 83593484

GI number: 83593484

Start: 2496163

End: 2498832

Strand: Direct

Name: rne [H]

Synonym: Rru_A2149

Alternate gene names: 83593484

Gene position: 2496163-2498832 (Clockwise)

Preceding gene: 83593481

Following gene: 83593485

Centisome position: 57.35

GC content: 67.34

Gene sequence:

>2670_bases
ATGACCAAGCGCATGCTTATCGATGCGAACCACCCCGAAGAAACGCGGGTCGTGGTTCTGGACGGTACCCGTCTCGACGA
GTTTGACGTCGAGACTTCAACGAAGAAACAGCTTAAGGGAAACATCTACCTCGCCAAAGTCGTCCGCGTCGAACCCTCGC
TCCAGGCCGCTTTCGTCGAATACGGCGGCAATCGCCATGGCTTCCTGGCCTTCAACGAGATCCACCCGGACTATTACCAG
ATCCCCGTCGCCGACCGCGAAAAGCTGATCGCCGAGGAGGCGCTTGAAGCCGCCGCCGATGACGATGACGAGGCCGACAG
CGTCGAGACCCTGGGCGGCGATGAAGCCGAAGACTCGGCCCCCGCTCCGCGCGCCAAATCGCGGGTCAGCCGCAGCTATA
AGATCCAAGAGGTGATCAAGCGCCGGCAGATCATGCTGGTGCAGGTCGTCAAGGAGGAGCGCGGCAACAAGGGCGCGGCT
CTGACCACCTATCTGTCGCTGGCCGGGCGCTATTGCGTGTTCATGCCCAATACCACGCGCGGCGGCGGCGTTTCGCGCAA
GATCACCAGCGCCACCGATCGCAAGCGCTTGAAGTCGATCATGGGCGAATTGATCGGCCCCAAGGGCAGCGCCGTCATCT
TGCGCACGGCGGGCAGCGAGCGGTCGCGGGCCGAAATCCGCCGCGACCATGACTATCTGCTGCGCACCTGGGATTCCATC
CGGCAGACGACGCTGGCCTCGCGCGCCCCTTGCCTGATCCACGAGGAAGGCAGCCTGATCAAGCGCGCCATCCGCGATAT
CTATTCCCGCGACATCGAGGAAATCCTGGTCGAAGGCGATGCCGGTTACCGCACCGCCAAGGATTTCATGAAGATGCTGA
CGCCTTCGCACGCCAAGAAGGTCCAGCCCTACAAAGACGAGATCATGCCGCTTTTCCACCGCTATCAGGTGGAAAGCCAG
ATGGATGCCATGCATTCGCCGATCGCCCAGTTGCGCTCGGGCGGCTATCTGGTCATCAACCAAACCGAGGCGCTGGTCGC
CATCGACGTCAATTCGGGCCGCTCGACGCGCGAGCGCAATATCGAAGAGACCGCCTATAAGACCAATCTGGAAGCCGCCG
ACGAAATCGCCCGCCAGCTGCGCCTGCGCGACCTGTCCGGGCTGATCGTCATCGACTTCATCGATATGGACGATCCGCGC
TCCAACGCCAATGTCGAGCGCCGGGTCAAGGACGCGCTGCGCAACGACCGCGCCCGCATCCAGCTTGGCCGCATCAGCCA
TTTCGGCCTGCTTGAAATGTCGCGCCAGCGGCTGCGCCCCAGCCTGATGGAAACCAGCTTCCAGCCCTGCCCGCATTGCC
ATGGCTCGGGGTCGCTGCGCTCGGTCGAATCCACGGCCATCCATATCCTGCGCATCATCGAGGAAGAGGGCGTGCGCCGG
CGTTCGAGCGAGGTCACGGTCACCGTGCCGACGGCGATCGCCCTTTACATCCTAAACAACAAGCGCACCGTTCTGGCCGC
CATCGAGGCGCGCTATGACTTCCAGGTGTTCTTGCTGGGCGACGACACGCTGATCGTTCCCGATTATCGCATGGACCGGG
TGAAGGCCGAGCGCGAGGGCTTGGATGCCCTGCCCGCCGCCGCCCCCCCGCCGACCGCGCCCGTCGCCGCCCTGCCGGCG
CCCGAGGTCCTGGAAGACGAGGACGAGGACGAGGGCACCGTCGAGGACGAGGCCTTCGCCGAAGAGAACGGCCAAAACGG
CGCCGATGCCGATGGACAGGGCCGCAAGCGCCGCCGCCGTCGCCGCCGTCGCCGCAAGGATCAGGACGGCGTGGAGATCG
AAGGCTCGTCCGTCGACGACGAAGAGGGCGCGCCCGAAGGCACGGCTGACTCGACCGAGGACGAGGGCGCCGATGACGAG
GGTGAGACCGGCGACGAGGCCGAGGACACGGGCGACGAGTTGGACCCGGCCGACGGGTCGCTGAGCGATCGTGATGGCCA
GTCGCGCCGCCGCCGCCGTCGCCGCGGCCGTCGCGGTCGTCGCGACGACACGGGCAACGGCTTGCCCGAGCCCGCCGTGC
CGGCCTCGACCGCCGATACCCTGCCCCTTGATGACGACTCGATCGAAAGCGCCGAGATCGACGACGATCCGACCGCCGAG
GACGAGATCATCTCGTCGACCGACGCGGACAGCGCCCCGGCCGGCGAGGACTCTGCGGCGGCGATCACCGATGAGCTTGG
CCGCGAGGGTCGCCGTCCGCCGCGTCGCCGTCGCCCCCGCCGCAAGGATCGGGGCGTCGAGACCAACCGCGCCATCGCCC
TTGACGCCAGCGACGCCGAAGCCGGCCCGGCGACCGCCGAAACGGATCTCGCCGCCTTCGACGACGCCCCGGACGCCGAG
GCGCCCTTCGCTGAGCCCTTGGGCGATGAGCGGTTGCTCGACGAGACCCCCGCCGAGATCGCCGAGGCCGAGGCCGAAGT
CGAGACCGCCGAGACGGACATCGACCGCGACCCCGCCGACGGGGAACCGCCGACCGCACCCGTCGCGCCGCCGCCGGTCG
CCGTCGTCAAGACCCCCGACCCCGCGGCCGCCGCCGCCCCGCCGGCCGCGGTCCCGTCTTCCGACCAACCCCGCCGCGTT
GGCTGGTGGTCGAAACTGATGTCGTCCTGA

Upstream 100 bases:

>100_bases
CAGCCCTCCGGCATCAAGAAGGCCCGCCGATCCCATCTGATCGACCGGCCACCCGTGGCGAAAAGCCCTGGTGGACCGGC
CTCGCGGAGTATCCTTCCGA

Downstream 100 bases:

>100_bases
TCGCGGCGCCGCCCGCCGACGATCACCCGATGACGGAGGGGGACCGGAGCAATCCGGTCCCCCTTTTAGTTGACGGCCCA
TGCAGACAGCCGATCTGGAC

Product: RNAse E

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 889; Mature: 888

Protein sequence:

>889_residues
MTKRMLIDANHPEETRVVVLDGTRLDEFDVETSTKKQLKGNIYLAKVVRVEPSLQAAFVEYGGNRHGFLAFNEIHPDYYQ
IPVADREKLIAEEALEAAADDDDEADSVETLGGDEAEDSAPAPRAKSRVSRSYKIQEVIKRRQIMLVQVVKEERGNKGAA
LTTYLSLAGRYCVFMPNTTRGGGVSRKITSATDRKRLKSIMGELIGPKGSAVILRTAGSERSRAEIRRDHDYLLRTWDSI
RQTTLASRAPCLIHEEGSLIKRAIRDIYSRDIEEILVEGDAGYRTAKDFMKMLTPSHAKKVQPYKDEIMPLFHRYQVESQ
MDAMHSPIAQLRSGGYLVINQTEALVAIDVNSGRSTRERNIEETAYKTNLEAADEIARQLRLRDLSGLIVIDFIDMDDPR
SNANVERRVKDALRNDRARIQLGRISHFGLLEMSRQRLRPSLMETSFQPCPHCHGSGSLRSVESTAIHILRIIEEEGVRR
RSSEVTVTVPTAIALYILNNKRTVLAAIEARYDFQVFLLGDDTLIVPDYRMDRVKAEREGLDALPAAAPPPTAPVAALPA
PEVLEDEDEDEGTVEDEAFAEENGQNGADADGQGRKRRRRRRRRRKDQDGVEIEGSSVDDEEGAPEGTADSTEDEGADDE
GETGDEAEDTGDELDPADGSLSDRDGQSRRRRRRRGRRGRRDDTGNGLPEPAVPASTADTLPLDDDSIESAEIDDDPTAE
DEIISSTDADSAPAGEDSAAAITDELGREGRRPPRRRRPRRKDRGVETNRAIALDASDAEAGPATAETDLAAFDDAPDAE
APFAEPLGDERLLDETPAEIAEAEAEVETAETDIDRDPADGEPPTAPVAPPPVAVVKTPDPAAAAAPPAAVPSSDQPRRV
GWWSKLMSS

Sequences:

>Translated_889_residues
MTKRMLIDANHPEETRVVVLDGTRLDEFDVETSTKKQLKGNIYLAKVVRVEPSLQAAFVEYGGNRHGFLAFNEIHPDYYQ
IPVADREKLIAEEALEAAADDDDEADSVETLGGDEAEDSAPAPRAKSRVSRSYKIQEVIKRRQIMLVQVVKEERGNKGAA
LTTYLSLAGRYCVFMPNTTRGGGVSRKITSATDRKRLKSIMGELIGPKGSAVILRTAGSERSRAEIRRDHDYLLRTWDSI
RQTTLASRAPCLIHEEGSLIKRAIRDIYSRDIEEILVEGDAGYRTAKDFMKMLTPSHAKKVQPYKDEIMPLFHRYQVESQ
MDAMHSPIAQLRSGGYLVINQTEALVAIDVNSGRSTRERNIEETAYKTNLEAADEIARQLRLRDLSGLIVIDFIDMDDPR
SNANVERRVKDALRNDRARIQLGRISHFGLLEMSRQRLRPSLMETSFQPCPHCHGSGSLRSVESTAIHILRIIEEEGVRR
RSSEVTVTVPTAIALYILNNKRTVLAAIEARYDFQVFLLGDDTLIVPDYRMDRVKAEREGLDALPAAAPPPTAPVAALPA
PEVLEDEDEDEGTVEDEAFAEENGQNGADADGQGRKRRRRRRRRRKDQDGVEIEGSSVDDEEGAPEGTADSTEDEGADDE
GETGDEAEDTGDELDPADGSLSDRDGQSRRRRRRRGRRGRRDDTGNGLPEPAVPASTADTLPLDDDSIESAEIDDDPTAE
DEIISSTDADSAPAGEDSAAAITDELGREGRRPPRRRRPRRKDRGVETNRAIALDASDAEAGPATAETDLAAFDDAPDAE
APFAEPLGDERLLDETPAEIAEAEAEVETAETDIDRDPADGEPPTAPVAPPPVAVVKTPDPAAAAAPPAAVPSSDQPRRV
GWWSKLMSS
>Mature_888_residues
TKRMLIDANHPEETRVVVLDGTRLDEFDVETSTKKQLKGNIYLAKVVRVEPSLQAAFVEYGGNRHGFLAFNEIHPDYYQI
PVADREKLIAEEALEAAADDDDEADSVETLGGDEAEDSAPAPRAKSRVSRSYKIQEVIKRRQIMLVQVVKEERGNKGAAL
TTYLSLAGRYCVFMPNTTRGGGVSRKITSATDRKRLKSIMGELIGPKGSAVILRTAGSERSRAEIRRDHDYLLRTWDSIR
QTTLASRAPCLIHEEGSLIKRAIRDIYSRDIEEILVEGDAGYRTAKDFMKMLTPSHAKKVQPYKDEIMPLFHRYQVESQM
DAMHSPIAQLRSGGYLVINQTEALVAIDVNSGRSTRERNIEETAYKTNLEAADEIARQLRLRDLSGLIVIDFIDMDDPRS
NANVERRVKDALRNDRARIQLGRISHFGLLEMSRQRLRPSLMETSFQPCPHCHGSGSLRSVESTAIHILRIIEEEGVRRR
SSEVTVTVPTAIALYILNNKRTVLAAIEARYDFQVFLLGDDTLIVPDYRMDRVKAEREGLDALPAAAPPPTAPVAALPAP
EVLEDEDEDEGTVEDEAFAEENGQNGADADGQGRKRRRRRRRRRKDQDGVEIEGSSVDDEEGAPEGTADSTEDEGADDEG
ETGDEAEDTGDELDPADGSLSDRDGQSRRRRRRRGRRGRRDDTGNGLPEPAVPASTADTLPLDDDSIESAEIDDDPTAED
EIISSTDADSAPAGEDSAAAITDELGREGRRPPRRRRPRRKDRGVETNRAIALDASDAEAGPATAETDLAAFDDAPDAEA
PFAEPLGDERLLDETPAEIAEAEAEVETAETDIDRDPADGEPPTAPVAPPPVAVVKTPDPAAAAAPPAAVPSSDQPRRVG
WWSKLMSS

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=535, Percent_Identity=45.2336448598131, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI87082235, Length=471, Percent_Identity=33.1210191082803, Blast_Score=238, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 97892; Mature: 97761

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRMLIDANHPEETRVVVLDGTRLDEFDVETSTKKQLKGNIYLAKVVRVEPSLQAAFVE
CCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHCCCEEEEEEEEECCCHHHHHHH
YGGNRHGFLAFNEIHPDYYQIPVADREKLIAEEALEAAADDDDEADSVETLGGDEAEDSA
HCCCCCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCC
PAPRAKSRVSRSYKIQEVIKRRQIMLVQVVKEERGNKGAALTTYLSLAGRYCVFMPNTTR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCCC
GGGVSRKITSATDRKRLKSIMGELIGPKGSAVILRTAGSERSRAEIRRDHDYLLRTWDSI
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
RQTTLASRAPCLIHEEGSLIKRAIRDIYSRDIEEILVEGDAGYRTAKDFMKMLTPSHAKK
HHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHH
VQPYKDEIMPLFHRYQVESQMDAMHSPIAQLRSGGYLVINQTEALVAIDVNSGRSTRERN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCHHHCC
IEETAYKTNLEAADEIARQLRLRDLSGLIVIDFIDMDDPRSNANVERRVKDALRNDRARI
HHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEE
QLGRISHFGLLEMSRQRLRPSLMETSFQPCPHCHGSGSLRSVESTAIHILRIIEEEGVRR
EECCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RSSEVTVTVPTAIALYILNNKRTVLAAIEARYDFQVFLLGDDTLIVPDYRMDRVKAEREG
CCCCEEEEECCEEEEEEECCCCEEEEEEECCCCEEEEEECCCEEEECCCHHHHHHHHHCC
LDALPAAAPPPTAPVAALPAPEVLEDEDEDEGTVEDEAFAEENGQNGADADGQGRKRRRR
CCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHH
RRRRRKDQDGVEIEGSSVDDEEGAPEGTADSTEDEGADDEGETGDEAEDTGDELDPADGS
HHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCC
LSDRDGQSRRRRRRRGRRGRRDDTGNGLPEPAVPASTADTLPLDDDSIESAEIDDDPTAE
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
DEIISSTDADSAPAGEDSAAAITDELGREGRRPPRRRRPRRKDRGVETNRAIALDASDAE
HHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHCCCCHHHCCCCCCCEEEEECCCCC
AGPATAETDLAAFDDAPDAEAPFAEPLGDERLLDETPAEIAEAEAEVETAETDIDRDPAD
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCC
GEPPTAPVAPPPVAVVKTPDPAAAAAPPAAVPSSDQPRRVGWWSKLMSS
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TKRMLIDANHPEETRVVVLDGTRLDEFDVETSTKKQLKGNIYLAKVVRVEPSLQAAFVE
CCEEEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHCCCEEEEEEEEECCCHHHHHHH
YGGNRHGFLAFNEIHPDYYQIPVADREKLIAEEALEAAADDDDEADSVETLGGDEAEDSA
HCCCCCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCC
PAPRAKSRVSRSYKIQEVIKRRQIMLVQVVKEERGNKGAALTTYLSLAGRYCVFMPNTTR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCCC
GGGVSRKITSATDRKRLKSIMGELIGPKGSAVILRTAGSERSRAEIRRDHDYLLRTWDSI
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
RQTTLASRAPCLIHEEGSLIKRAIRDIYSRDIEEILVEGDAGYRTAKDFMKMLTPSHAKK
HHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHH
VQPYKDEIMPLFHRYQVESQMDAMHSPIAQLRSGGYLVINQTEALVAIDVNSGRSTRERN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCHHHCC
IEETAYKTNLEAADEIARQLRLRDLSGLIVIDFIDMDDPRSNANVERRVKDALRNDRARI
HHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEE
QLGRISHFGLLEMSRQRLRPSLMETSFQPCPHCHGSGSLRSVESTAIHILRIIEEEGVRR
EECCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RSSEVTVTVPTAIALYILNNKRTVLAAIEARYDFQVFLLGDDTLIVPDYRMDRVKAEREG
CCCCEEEEECCEEEEEEECCCCEEEEEEECCCCEEEEEECCCEEEECCCHHHHHHHHHCC
LDALPAAAPPPTAPVAALPAPEVLEDEDEDEGTVEDEAFAEENGQNGADADGQGRKRRRR
CCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHH
RRRRRKDQDGVEIEGSSVDDEEGAPEGTADSTEDEGADDEGETGDEAEDTGDELDPADGS
HHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCC
LSDRDGQSRRRRRRRGRRGRRDDTGNGLPEPAVPASTADTLPLDDDSIESAEIDDDPTAE
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
DEIISSTDADSAPAGEDSAAAITDELGREGRRPPRRRRPRRKDRGVETNRAIALDASDAE
HHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHCCCCHHHCCCCCCCEEEEECCCCC
AGPATAETDLAAFDDAPDAEAPFAEPLGDERLLDETPAEIAEAEAEVETAETDIDRDPAD
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCC
GEPPTAPVAPPPVAVVKTPDPAAAAAPPAAVPSSDQPRRVGWWSKLMSS
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1447789; 1704367; 2011493; 8415644 [H]