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The map label for this gene is gltK [H]

Identifier: 83593424

GI number: 83593424

Start: 2417532

End: 2418224

Strand: Direct

Name: gltK [H]

Synonym: Rru_A2089

Alternate gene names: 83593424

Gene position: 2417532-2418224 (Clockwise)

Preceding gene: 83593423

Following gene: 83593425

Centisome position: 55.54

GC content: 60.89

Gene sequence:

>693_bases
ATGTTCGCAACCCTCGACTTCGATGTCATCGCCCGCTCGATCGGCTATCTGTTCTATGACGGGATGACCTTCACCCTGGG
GCTGACCTTGCTCGGCACCCTGGCCGGCGGGCTGCTTGGCACGGCCTTGGCCATGCTGCGGCTGTCCAGCCTGCCCGTTC
TTCCCCATCTGGCGACGCTCTATATCAATCTGATGCGCTCGATGCCGCTGGTTCTGTTGATTTTCTGGGTCTACTTCATG
GTCCCCTATCTGGGGCAATGGATCTTGGGAACCGATCGGCCGATGCAGGTCGGCACCTTCACCTCGTCGCTGCTAACCTT
CACCCTGTTCGAAGCGGCCTATTTCGCCGAGATCATGCGCTCGGGCATCCAATCGGTCTCGCGCGGGCAGACGGCGGCGG
CCCAGGCCCTTGGCCTCACCTATTTCCAGTCGATGGGCCATGTGGTGCTGCCCCAGGCGTTCCGTGCCATGCTGCCGGTG
CTTCTGACCCAGGTGATCGTGTTATTTCAGGACACCTCGCTGGTCTATGTGCTGTCGATCACCGATTTCCTTGGTGCGGC
GAGCAAAGTGGCGCAGCGCGATGGCCGGCTGGTCGAAATGTACCTTTTCGCCGCCGTCGTCTATTTCGCCATCTCGTTCG
CCGCCTCGCTGTTGGTCAAGCGCATTCAGGCGCGCACCGCTATCATTCGCTGA

Upstream 100 bases:

>100_bases
TCTACGTTCTGATCAACGTGGTGGTGGTCGCCGTCATGCGTGTGATCGAGCATCGTGTCGCGGTGCCCGGTTTCAACGTC
GGCAAGTAAGGAAAACGCCG

Downstream 100 bases:

>100_bases
TGAAGGCCCCAGAGATGATCGAAATCACCCACGTTGATAAATGGTATGGGACCTTTCAGGTTCTGAAGGATTGCACCACC
CAGGTTGCCAAGGGCGAGGT

Product: amino acid ABC transporter permease

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MFATLDFDVIARSIGYLFYDGMTFTLGLTLLGTLAGGLLGTALAMLRLSSLPVLPHLATLYINLMRSMPLVLLIFWVYFM
VPYLGQWILGTDRPMQVGTFTSSLLTFTLFEAAYFAEIMRSGIQSVSRGQTAAAQALGLTYFQSMGHVVLPQAFRAMLPV
LLTQVIVLFQDTSLVYVLSITDFLGAASKVAQRDGRLVEMYLFAAVVYFAISFAASLLVKRIQARTAIIR

Sequences:

>Translated_230_residues
MFATLDFDVIARSIGYLFYDGMTFTLGLTLLGTLAGGLLGTALAMLRLSSLPVLPHLATLYINLMRSMPLVLLIFWVYFM
VPYLGQWILGTDRPMQVGTFTSSLLTFTLFEAAYFAEIMRSGIQSVSRGQTAAAQALGLTYFQSMGHVVLPQAFRAMLPV
LLTQVIVLFQDTSLVYVLSITDFLGAASKVAQRDGRLVEMYLFAAVVYFAISFAASLLVKRIQARTAIIR
>Mature_230_residues
MFATLDFDVIARSIGYLFYDGMTFTLGLTLLGTLAGGLLGTALAMLRLSSLPVLPHLATLYINLMRSMPLVLLIFWVYFM
VPYLGQWILGTDRPMQVGTFTSSLLTFTLFEAAYFAEIMRSGIQSVSRGQTAAAQALGLTYFQSMGHVVLPQAFRAMLPV
LLTQVIVLFQDTSLVYVLSITDFLGAASKVAQRDGRLVEMYLFAAVVYFAISFAASLLVKRIQARTAIIR

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786873, Length=222, Percent_Identity=52.2522522522522, Blast_Score=216, Evalue=8e-58,
Organism=Escherichia coli, GI87082239, Length=212, Percent_Identity=32.0754716981132, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788226, Length=206, Percent_Identity=32.0388349514563, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787030, Length=214, Percent_Identity=29.9065420560748, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786874, Length=199, Percent_Identity=29.6482412060301, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788645, Length=217, Percent_Identity=29.0322580645161, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1787087, Length=130, Percent_Identity=32.3076923076923, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI48994930, Length=166, Percent_Identity=30.7228915662651, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 25353; Mature: 25353

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFATLDFDVIARSIGYLFYDGMTFTLGLTLLGTLAGGLLGTALAMLRLSSLPVLPHLATL
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
YINLMRSMPLVLLIFWVYFMVPYLGQWILGTDRPMQVGTFTSSLLTFTLFEAAYFAEIMR
HHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SGIQSVSRGQTAAAQALGLTYFQSMGHVVLPQAFRAMLPVLLTQVIVLFQDTSLVYVLSI
HHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHH
TDFLGAASKVAQRDGRLVEMYLFAAVVYFAISFAASLLVKRIQARTAIIR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFATLDFDVIARSIGYLFYDGMTFTLGLTLLGTLAGGLLGTALAMLRLSSLPVLPHLATL
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
YINLMRSMPLVLLIFWVYFMVPYLGQWILGTDRPMQVGTFTSSLLTFTLFEAAYFAEIMR
HHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SGIQSVSRGQTAAAQALGLTYFQSMGHVVLPQAFRAMLPVLLTQVIVLFQDTSLVYVLSI
HHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHH
TDFLGAASKVAQRDGRLVEMYLFAAVVYFAISFAASLLVKRIQARTAIIR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]