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The map label for this gene is acrA [H]

Identifier: 83593385

GI number: 83593385

Start: 2370148

End: 2371428

Strand: Direct

Name: acrA [H]

Synonym: Rru_A2050

Alternate gene names: 83593385

Gene position: 2370148-2371428 (Clockwise)

Preceding gene: 83593377

Following gene: 83593386

Centisome position: 54.45

GC content: 66.9

Gene sequence:

>1281_bases
ATGTGTACTGTACCGTACAGTTTACTGACCGTCCACGCCTTTCGCCGTGACGGCACACCGACACACCAAGAGATGCCCCC
CATGATGCTCAGCAAGACATTCCTGCGCCTGATGACGACCGCCGCCCTGTCGGCGGTTCTATGGAGCTGCGACTCGCAGG
AAGGGGGGCAGGCGGCGGGTGGCGCCCGACCGGCCCCGGAAGTCACGGTGATCACCGCCGCTCCGCAAACCTTGCGGATC
ACCGAACGGCTTCCCGGTCGCACGGTGGCCTACCGCAGCGCCGAGGTCCGGCCCCAGGTCAACGGCATCATCCTCAAGCG
CCTGTTCGAGGAGGGCACCGAGGTCACCGAGGGCCAGCAGCTTTATCAGATCGACCCGGCCACCTATAAGGCGGCCCTCG
ATAGCGCCAAGGCCCAGCTCGCCAAGGCCGACGCCACGGTCAAATCGGCCCGGGCCAAGGCGGCGCGCTATGGCGAGTTG
GTCAAGCTCAAGGCGGTCAGCCGTCAGGATTATGACGACGTCACCGCCACCCTGGCCGAAAGCGTCGCCTCGGTCGCCGA
TGCCCAGGCGACCGTTGACGCCGCCGAAATCAATTTGGCCTATACCCGGGTCACCGCGCCGATTTCCGGGCGGATCGGCC
GCTCGGCGGTCACCGAGGGCGCCCTGGTGACGGCCAATCAGGCCACGACCCTGGCGACGATCACCCAGCTTGATCCCATC
AACGTCGATTTGACCCAGTCGAGCGACAAACTGCTCAAGCTGCGCGCCCAGATGGCCAATGGCACGGTTCAGGCCCCGGA
GCGGGTTCCCGTTACCCTGCAGATCGATAATTCCGGCACGCCCTACGGCCTGACCGGAACCCTGCAGTTTTCAGAGGTCG
TGGTTGACGAAACCACGGGCACGGTGCGCCTGCGCGCCACCTTCCCCAATCCCAACCGGATCTTACTGCCCGGATTGTTC
GTACGCGCCACCGTTGATTTCGGCAGCCGCGAGAACGTCTTTCTGATTCCCCAGCGCGCCGTCGTCCGTGACGCCTCGGG
CAAGGCCGCCCTATGGGTGATCGGCGACGGCGACAAGGCCGAACGCCGGCCGGTTACCGCCGAAACCACCCAGGGCACCA
ATTGGGTGGTCACCGAGGGCCTGAAGGGCGGCGAGCGCATCGTCATCGCCGGATTGCAAAGCATGGCGCCCGGCGCCCAG
GTCAAGGCCGTCGCCGCCCCGCCCGCGGGCCAGGAGGCGGGGCAACCCGCCGGCGGCCAGCCCGCGCCCGCCGCGAAATA
A

Upstream 100 bases:

>100_bases
CGCGCGTCTCCCCGCCCGCAGGGAGATCCCCTTTTTTCCAACGCCTCGTCACCTCCTTTTTCACCGCCGCGACTTTTTGG
CTCTTGTACGCCGCGACAGG

Downstream 100 bases:

>100_bases
TCGGGGACCCCGTCCATGGCCAGTTTCTTTATCGATCGCCCCGTCTTCGCATGGGTCATCGCCATCATCATCATGCTCTC
CGGGGCGCTCTCCATCCTCT

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MCTVPYSLLTVHAFRRDGTPTHQEMPPMMLSKTFLRLMTTAALSAVLWSCDSQEGGQAAGGARPAPEVTVITAAPQTLRI
TERLPGRTVAYRSAEVRPQVNGIILKRLFEEGTEVTEGQQLYQIDPATYKAALDSAKAQLAKADATVKSARAKAARYGEL
VKLKAVSRQDYDDVTATLAESVASVADAQATVDAAEINLAYTRVTAPISGRIGRSAVTEGALVTANQATTLATITQLDPI
NVDLTQSSDKLLKLRAQMANGTVQAPERVPVTLQIDNSGTPYGLTGTLQFSEVVVDETTGTVRLRATFPNPNRILLPGLF
VRATVDFGSRENVFLIPQRAVVRDASGKAALWVIGDGDKAERRPVTAETTQGTNWVVTEGLKGGERIVIAGLQSMAPGAQ
VKAVAAPPAGQEAGQPAGGQPAPAAK

Sequences:

>Translated_426_residues
MCTVPYSLLTVHAFRRDGTPTHQEMPPMMLSKTFLRLMTTAALSAVLWSCDSQEGGQAAGGARPAPEVTVITAAPQTLRI
TERLPGRTVAYRSAEVRPQVNGIILKRLFEEGTEVTEGQQLYQIDPATYKAALDSAKAQLAKADATVKSARAKAARYGEL
VKLKAVSRQDYDDVTATLAESVASVADAQATVDAAEINLAYTRVTAPISGRIGRSAVTEGALVTANQATTLATITQLDPI
NVDLTQSSDKLLKLRAQMANGTVQAPERVPVTLQIDNSGTPYGLTGTLQFSEVVVDETTGTVRLRATFPNPNRILLPGLF
VRATVDFGSRENVFLIPQRAVVRDASGKAALWVIGDGDKAERRPVTAETTQGTNWVVTEGLKGGERIVIAGLQSMAPGAQ
VKAVAAPPAGQEAGQPAGGQPAPAAK
>Mature_426_residues
MCTVPYSLLTVHAFRRDGTPTHQEMPPMMLSKTFLRLMTTAALSAVLWSCDSQEGGQAAGGARPAPEVTVITAAPQTLRI
TERLPGRTVAYRSAEVRPQVNGIILKRLFEEGTEVTEGQQLYQIDPATYKAALDSAKAQLAKADATVKSARAKAARYGEL
VKLKAVSRQDYDDVTATLAESVASVADAQATVDAAEINLAYTRVTAPISGRIGRSAVTEGALVTANQATTLATITQLDPI
NVDLTQSSDKLLKLRAQMANGTVQAPERVPVTLQIDNSGTPYGLTGTLQFSEVVVDETTGTVRLRATFPNPNRILLPGLF
VRATVDFGSRENVFLIPQRAVVRDASGKAALWVIGDGDKAERRPVTAETTQGTNWVVTEGLKGGERIVIAGLQSMAPGAQ
VKAVAAPPAGQEAGQPAGGQPAPAAK

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=368, Percent_Identity=51.6304347826087, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1789665, Length=376, Percent_Identity=47.8723404255319, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1789929, Length=363, Percent_Identity=48.4848484848485, Blast_Score=329, Evalue=2e-91,
Organism=Escherichia coli, GI87082036, Length=326, Percent_Identity=31.9018404907975, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI87081796, Length=356, Percent_Identity=27.5280898876405, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44980; Mature: 44980

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTVPYSLLTVHAFRRDGTPTHQEMPPMMLSKTFLRLMTTAALSAVLWSCDSQEGGQAAG
CCCCCHHHHEEEEHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GARPAPEVTVITAAPQTLRITERLPGRTVAYRSAEVRPQVNGIILKRLFEEGTEVTEGQQ
CCCCCCCEEEEECCCCEEEEHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCE
LYQIDPATYKAALDSAKAQLAKADATVKSARAKAARYGELVKLKAVSRQDYDDVTATLAE
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHH
SVASVADAQATVDAAEINLAYTRVTAPISGRIGRSAVTEGALVTANQATTLATITQLDPI
HHHHHHHHHHEEEHHHEEEEEEEEECCCCCCCCCHHHCCCEEEECCCCEEEEEEEECCCC
NVDLTQSSDKLLKLRAQMANGTVQAPERVPVTLQIDNSGTPYGLTGTLQFSEVVVDETTG
EEEEECCCHHHHHHHHHHCCCCEECCCCCEEEEEECCCCCCEEEEEEEEEEEEEEECCCC
TVRLRATFPNPNRILLPGLFVRATVDFGSRENVFLIPQRAVVRDASGKAALWVIGDGDKA
EEEEEEECCCCCEEEECCEEEEEEECCCCCCCEEEECCHHEEECCCCCEEEEEEECCCCC
ERRPVTAETTQGTNWVVTEGLKGGERIVIAGLQSMAPGAQVKAVAAPPAGQEAGQPAGGQ
CCCCCCEECCCCCCEEEECCCCCCCEEEEEEHHHCCCCCEEEEEECCCCCCCCCCCCCCC
PAPAAK
CCCCCC
>Mature Secondary Structure
MCTVPYSLLTVHAFRRDGTPTHQEMPPMMLSKTFLRLMTTAALSAVLWSCDSQEGGQAAG
CCCCCHHHHEEEEHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GARPAPEVTVITAAPQTLRITERLPGRTVAYRSAEVRPQVNGIILKRLFEEGTEVTEGQQ
CCCCCCCEEEEECCCCEEEEHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCE
LYQIDPATYKAALDSAKAQLAKADATVKSARAKAARYGELVKLKAVSRQDYDDVTATLAE
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHH
SVASVADAQATVDAAEINLAYTRVTAPISGRIGRSAVTEGALVTANQATTLATITQLDPI
HHHHHHHHHHEEEHHHEEEEEEEEECCCCCCCCCHHHCCCEEEECCCCEEEEEEEECCCC
NVDLTQSSDKLLKLRAQMANGTVQAPERVPVTLQIDNSGTPYGLTGTLQFSEVVVDETTG
EEEEECCCHHHHHHHHHHCCCCEECCCCCEEEEEECCCCCCEEEEEEEEEEEEEEECCCC
TVRLRATFPNPNRILLPGLFVRATVDFGSRENVFLIPQRAVVRDASGKAALWVIGDGDKA
EEEEEEECCCCCEEEECCEEEEEEECCCCCCCEEEECCHHEEECCCCCEEEEEEECCCCC
ERRPVTAETTQGTNWVVTEGLKGGERIVIAGLQSMAPGAQVKAVAAPPAGQEAGQPAGGQ
CCCCCCEECCCCCCEEEECCCCCCCEEEEEEHHHCCCCCEEEEEECCCCCCCCCCCCCCC
PAPAAK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]