Click here to switch to the map view.

The map label for this gene is nifS [H]

Identifier: 83593362

GI number: 83593362

Start: 2340150

End: 2341256

Strand: Reverse

Name: nifS [H]

Synonym: Rru_A2027

Alternate gene names: 83593362

Gene position: 2341256-2340150 (Counterclockwise)

Preceding gene: 83593363

Following gene: 83593361

Centisome position: 53.79

GC content: 72.27

Gene sequence:

>1107_bases
GTGACCCACGCCGGCGTCTATCTCGACCACAACGCCACCACGGCGCTGCGCCCCGAAGCGGTCGCGGCGATCACCGCCGC
CCTGGAGGTTCTTGGCAATCCGTCGAGCATCCACCGCGACGGACGGCGGGCCCGCGCCCTGATCGAGGATGCCCGCGACG
CCGTCGGCGCCCTTGTCGGCGCCCGGGCCGAGCGGGTGATCTTCACCTCGGGCGGTAGCGAGGCCAATGCCCTTGGTCTG
CTGGGAAGCGGTCGTCGGCTGATCGTCGGGGCGACGGAACATCCTTGCGTGCTGCGCGCCGATCCGGCCGCCCTGCGCAT
CCGCGTCGATCGCCACGGCGTTGTCGATCTTGGCCATCTCGAGGCCTTGCTGCAGGCCGAGCGCAACCCGGCGGTGGTCG
GGCTGATGCTGGCCAATAATGAAACCGGCGTCGTCCAGCCGGTGGCCGAGGCGGTGGCCCTGGCCCATGCCCATGGCGCC
CTGGTCCATTGCGACGGGGTTCAGGCGCCCGGGCGCATGGCGCTGTCGCTGGCGGCGCTGGGCTGTGACTCGCTTGCGCT
CTCGGGTCACAAGATCGGCGGGCCGGCCGGATCGGGCGCCCTGATCCTGGCCGAGGGCCACGAGGTGAGCCCCGGTTTGC
GCGGCGGCGGCCAGGAGCGCGGCCGGCGGGCCGGAACCGAGAATCTGCTTGGCATCGTCGGTTTTGGCGCCAGCGCCGCC
ACCCTGGGCGCCCAAACGGCGGCCGAGTCCACGCGCCTTGGCGCCTTGCGTGGGCGTTTGGAGGCGGCGGTGACGGCGGC
AGTCCCCGGGGTAGTGATTTACGGCGCCGGCGTCGCCCGCCTGCCCAACACCACCTGTGTCGGGCTGCCCGGCGTCGGCG
CCGATCGTCAGGTGATGGCCCTGGATCTCGCCGGGCTGCGGGTCAGCGCCGGCAGCGCCTGTTCCAGCGGCACCCTGTCG
GCCTCGCCGGTGCTGCTGGCGATGGGAGTGGGCGAGGACGCCGCCCGTCAGGCGATCCGGGTCAGTCTTGGCTGGACGAC
CACCGAGGACGAGGTGAGCGGCTTTATCGAGGCTTATGGGGCGATGGCCGCTCGTCTGACCGCGTGA

Upstream 100 bases:

>100_bases
ATCTTTATCTCAGCAGCGTCTCGCTTGAAGATGTCACCCAGCGCCGGGTCCTTGGCACCAGCGGCCTGATCGGCGAGATG
CCCCCCATCGTCGCGGTGCA

Downstream 100 bases:

>100_bases
TTTCGTCCCCGGGGCCTTGCTCCCCGGGCTTGGGAGAGGTATATCGCCGGGCAACCTTTCGCATCCGCTCAGGAGTTGAC
ATGCCCAAGGTCATTTTTAT

Product: aminotransferase, class V

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein NifS [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MTHAGVYLDHNATTALRPEAVAAITAALEVLGNPSSIHRDGRRARALIEDARDAVGALVGARAERVIFTSGGSEANALGL
LGSGRRLIVGATEHPCVLRADPAALRIRVDRHGVVDLGHLEALLQAERNPAVVGLMLANNETGVVQPVAEAVALAHAHGA
LVHCDGVQAPGRMALSLAALGCDSLALSGHKIGGPAGSGALILAEGHEVSPGLRGGGQERGRRAGTENLLGIVGFGASAA
TLGAQTAAESTRLGALRGRLEAAVTAAVPGVVIYGAGVARLPNTTCVGLPGVGADRQVMALDLAGLRVSAGSACSSGTLS
ASPVLLAMGVGEDAARQAIRVSLGWTTTEDEVSGFIEAYGAMAARLTA

Sequences:

>Translated_368_residues
MTHAGVYLDHNATTALRPEAVAAITAALEVLGNPSSIHRDGRRARALIEDARDAVGALVGARAERVIFTSGGSEANALGL
LGSGRRLIVGATEHPCVLRADPAALRIRVDRHGVVDLGHLEALLQAERNPAVVGLMLANNETGVVQPVAEAVALAHAHGA
LVHCDGVQAPGRMALSLAALGCDSLALSGHKIGGPAGSGALILAEGHEVSPGLRGGGQERGRRAGTENLLGIVGFGASAA
TLGAQTAAESTRLGALRGRLEAAVTAAVPGVVIYGAGVARLPNTTCVGLPGVGADRQVMALDLAGLRVSAGSACSSGTLS
ASPVLLAMGVGEDAARQAIRVSLGWTTTEDEVSGFIEAYGAMAARLTA
>Mature_367_residues
THAGVYLDHNATTALRPEAVAAITAALEVLGNPSSIHRDGRRARALIEDARDAVGALVGARAERVIFTSGGSEANALGLL
GSGRRLIVGATEHPCVLRADPAALRIRVDRHGVVDLGHLEALLQAERNPAVVGLMLANNETGVVQPVAEAVALAHAHGAL
VHCDGVQAPGRMALSLAALGCDSLALSGHKIGGPAGSGALILAEGHEVSPGLRGGGQERGRRAGTENLLGIVGFGASAAT
LGAQTAAESTRLGALRGRLEAAVTAAVPGVVIYGAGVARLPNTTCVGLPGVGADRQVMALDLAGLRVSAGSACSSGTLSA
SPVLLAMGVGEDAARQAIRVSLGWTTTEDEVSGFIEAYGAMAARLTA

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=383, Percent_Identity=33.9425587467363, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI156713448, Length=410, Percent_Identity=33.4146341463415, Blast_Score=172, Evalue=4e-43,
Organism=Escherichia coli, GI48994898, Length=373, Percent_Identity=35.3887399463807, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1789175, Length=396, Percent_Identity=26.7676767676768, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787970, Length=391, Percent_Identity=26.8542199488491, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25143064, Length=371, Percent_Identity=33.1536388140162, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17533177, Length=330, Percent_Identity=29.3939393939394, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319831, Length=372, Percent_Identity=32.5268817204301, Blast_Score=176, Evalue=4e-45,
Organism=Drosophila melanogaster, GI20129463, Length=366, Percent_Identity=34.6994535519126, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 36869; Mature: 36737

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHAGVYLDHNATTALRPEAVAAITAALEVLGNPSSIHRDGRRARALIEDARDAVGALVG
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHC
ARAERVIFTSGGSEANALGLLGSGRRLIVGATEHPCVLRADPAALRIRVDRHGVVDLGHL
CCCCEEEEECCCCCCCEEEEECCCCEEEEECCCCCEEEEECCCEEEEEECCCCCEEHHHH
EALLQAERNPAVVGLMLANNETGVVQPVAEAVALAHAHGALVHCDGVQAPGRMALSLAAL
HHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHEEEHHHH
GCDSLALSGHKIGGPAGSGALILAEGHEVSPGLRGGGQERGRRAGTENLLGIVGFGASAA
CCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHCCCCCCEEEEECCCCHH
TLGAQTAAESTRLGALRGRLEAAVTAAVPGVVIYGAGVARLPNTTCVGLPGVGADRQVMA
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHCCCCCEEEECCCCCCCCEEEE
LDLAGLRVSAGSACSSGTLSASPVLLAMGVGEDAARQAIRVSLGWTTTEDEVSGFIEAYG
EEECCEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHEEEEEECCCCCHHHHHHHHHHHH
AMAARLTA
HHHHCCCC
>Mature Secondary Structure 
THAGVYLDHNATTALRPEAVAAITAALEVLGNPSSIHRDGRRARALIEDARDAVGALVG
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHC
ARAERVIFTSGGSEANALGLLGSGRRLIVGATEHPCVLRADPAALRIRVDRHGVVDLGHL
CCCCEEEEECCCCCCCEEEEECCCCEEEEECCCCCEEEEECCCEEEEEECCCCCEEHHHH
EALLQAERNPAVVGLMLANNETGVVQPVAEAVALAHAHGALVHCDGVQAPGRMALSLAAL
HHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHEEEHHHH
GCDSLALSGHKIGGPAGSGALILAEGHEVSPGLRGGGQERGRRAGTENLLGIVGFGASAA
CCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHCCCCCCEEEEECCCCHH
TLGAQTAAESTRLGALRGRLEAAVTAAVPGVVIYGAGVARLPNTTCVGLPGVGADRQVMA
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHCCCCCEEEECCCCCCCCEEEE
LDLAGLRVSAGSACSSGTLSASPVLLAMGVGEDAARQAIRVSLGWTTTEDEVSGFIEAYG
EEECCEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHEEEEEECCCCCHHHHHHHHHHHH
AMAARLTA
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA