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The map label for this gene is asnB [H]

Identifier: 83593324

GI number: 83593324

Start: 2299706

End: 2301481

Strand: Reverse

Name: asnB [H]

Synonym: Rru_A1989

Alternate gene names: 83593324

Gene position: 2301481-2299706 (Counterclockwise)

Preceding gene: 83593325

Following gene: 83593323

Centisome position: 52.87

GC content: 62.95

Gene sequence:

>1776_bases
ATGTGTGGAGTGTGTGGAGAAATCCGGTTTGACGGCCAGTTCGCCGGAACAACCGCCGTAGACCGGATGACGGCCAAACT
GGCGCCCCGTGGTCCCGATGACGGAGGTATCCTGGTCCACGGACCGGTGGCGCTTGGGCATCGCCGGCTTAGCATCATCG
ATCTTTCGCCCAAGGCGGCTCAGCCGATGGTCGATTCCGATCTTGGTCTCAGCATCGTTTTCAACGGCTGCATCTATAAT
TATCCGGCGCTGCGCTCCGAGTTGGAGGCCCTTGGCTACCGGTTTTTCTCGGGCGGTGATACGGAGGTCATCCTCAAGGC
CTATCACGCCTGGGGAACGGCTTGCGTCAAGCGCTTCAACGGCATGTTCGCCTTCGCCATTCACGAACGCGACAGCGGCA
AGGTGGTTCTGGCCCGGGATCGCTTCGGCATCAAACCGCTGTATCTGGCCGAGGTCGACGGCGGTTTGCTGTTCGCGTCC
TCCTTGCCGGCCTTGCTGGCGACCGGACGCGTCGATAAGGCGATCGATCCGCTGGCCTTGCGGTTCTATATAAGCTTTCA
CGCCGTGGTGCCGGCGCCGCGCACCATCCTCAAGGGCGTGAGAAAACTGCCGCCGGCGACCTTGCGCGTTGTGTCGGCCG
ATGGCTCCAGCTTTGACGAGGTCTATTGGCGGCTTGATTTCCGCGCCTTGCCCGGAGAGGACGGTCGCTCGGCCAAGGAC
TGGGAGGATGGCCTGTTCGAGGCGGTGCGGGCGGCGGTGGCGCGGCGGATAATCGCCGATGTGCCCGTGGGCGTCTTGCT
TTCGGGCGGGCTGGATTCCAGCCTGATCGTCGGTTTGCTGGCCGAGGCCGGACAAAGGGATGTGGAAACCTTTTCCATCG
GCTTTGAAGAGGCCAACGGTGAAAAGGGCGACGAGTTTTTCTATTCCGACCTGATCGCCGAACGCTACGCCACCCGCCAC
CACAAGATCTTCATTCCCGAAGCCGACATGATCGCCGCCTTGCCCGGGGCGATCGCCGCGATGTCCGAGCCGATGGTCTC
CTATGACAACGTGGCGTTCTATCTGCTGGCCCGCGAGGTGTCCAAGCGGGTGAAGGTCGTTCTGTCGGGCCAGGGCGCCG
ATGAGATCTTTGGCGGCTATCACTGGTATCGGCCGCTTGTCGGCTCGACCGATCCGGTCGCCGATTACGCCGCCCATTTC
TTCGACCGCACCCCGGAGCGGCTGGCCCGCCACCTTTCCCCGGCTTGGCTGGGGACGACCGACGAGGCGCGGGCCTTCGT
CGCCGCCCATTTCGCCGAGGACGGAGCCCCTGATCCGGTCGATAAGGCGCTGCGCCTCGATAGCACCGTCATGCTGGTCG
AGGATCCGGTCAAACGCGTTGACAACATGACCATGGCCTTTGGCCTGGAAGCCCGCGTGCCGTTTCTCGATCATCAACTG
GCCGAATTCGCCGGGCGCATTCCCTCGTCGCTCAAGCTGGCCCAGAACGGTAAGGGCATTCTCAAGGAGGTCGCCCGCCG
GGTGGTTCCCGCCGCCGTGATCGATCGCGAGAAGGGCTATTTTCCGGTGCCGGGGCTGAAATACATCGAAGGATCGCGCC
TGGATTATGTGCGCGATGGCCTGTCAAGCCGGGCGGCGCGCCAGCGCGGCCTGTTTCGCGAGGACTATCTGGCCAGCCTT
TATGAAAATCCGCGCGACGCCATCACCCCCTTGCGCGGATCGGAGCTTTGGCAGGTCGGGCTTCTTGAACTTTGGCTGCA
AGAGCATGGGATTTGA

Upstream 100 bases:

>100_bases
TTTTGTTGGATTTTCTATAAAAAATCGTCGGATACACCAATAATTACTAAGAAATCATACCTGTATTTCTCTGGCGATTG
TTTGTTGTATTGGAATTCTT

Downstream 100 bases:

>100_bases
AGACCATGGACGATCTTTCCCACGAGTTCAAAAAAGAGGCCTCGATTGATTGCGGCTGGGGCCGGCTGGTCTTTGGTCAG
ACCTTCGACGGCCCCGGGCG

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MCGVCGEIRFDGQFAGTTAVDRMTAKLAPRGPDDGGILVHGPVALGHRRLSIIDLSPKAAQPMVDSDLGLSIVFNGCIYN
YPALRSELEALGYRFFSGGDTEVILKAYHAWGTACVKRFNGMFAFAIHERDSGKVVLARDRFGIKPLYLAEVDGGLLFAS
SLPALLATGRVDKAIDPLALRFYISFHAVVPAPRTILKGVRKLPPATLRVVSADGSSFDEVYWRLDFRALPGEDGRSAKD
WEDGLFEAVRAAVARRIIADVPVGVLLSGGLDSSLIVGLLAEAGQRDVETFSIGFEEANGEKGDEFFYSDLIAERYATRH
HKIFIPEADMIAALPGAIAAMSEPMVSYDNVAFYLLAREVSKRVKVVLSGQGADEIFGGYHWYRPLVGSTDPVADYAAHF
FDRTPERLARHLSPAWLGTTDEARAFVAAHFAEDGAPDPVDKALRLDSTVMLVEDPVKRVDNMTMAFGLEARVPFLDHQL
AEFAGRIPSSLKLAQNGKGILKEVARRVVPAAVIDREKGYFPVPGLKYIEGSRLDYVRDGLSSRAARQRGLFREDYLASL
YENPRDAITPLRGSELWQVGLLELWLQEHGI

Sequences:

>Translated_591_residues
MCGVCGEIRFDGQFAGTTAVDRMTAKLAPRGPDDGGILVHGPVALGHRRLSIIDLSPKAAQPMVDSDLGLSIVFNGCIYN
YPALRSELEALGYRFFSGGDTEVILKAYHAWGTACVKRFNGMFAFAIHERDSGKVVLARDRFGIKPLYLAEVDGGLLFAS
SLPALLATGRVDKAIDPLALRFYISFHAVVPAPRTILKGVRKLPPATLRVVSADGSSFDEVYWRLDFRALPGEDGRSAKD
WEDGLFEAVRAAVARRIIADVPVGVLLSGGLDSSLIVGLLAEAGQRDVETFSIGFEEANGEKGDEFFYSDLIAERYATRH
HKIFIPEADMIAALPGAIAAMSEPMVSYDNVAFYLLAREVSKRVKVVLSGQGADEIFGGYHWYRPLVGSTDPVADYAAHF
FDRTPERLARHLSPAWLGTTDEARAFVAAHFAEDGAPDPVDKALRLDSTVMLVEDPVKRVDNMTMAFGLEARVPFLDHQL
AEFAGRIPSSLKLAQNGKGILKEVARRVVPAAVIDREKGYFPVPGLKYIEGSRLDYVRDGLSSRAARQRGLFREDYLASL
YENPRDAITPLRGSELWQVGLLELWLQEHGI
>Mature_591_residues
MCGVCGEIRFDGQFAGTTAVDRMTAKLAPRGPDDGGILVHGPVALGHRRLSIIDLSPKAAQPMVDSDLGLSIVFNGCIYN
YPALRSELEALGYRFFSGGDTEVILKAYHAWGTACVKRFNGMFAFAIHERDSGKVVLARDRFGIKPLYLAEVDGGLLFAS
SLPALLATGRVDKAIDPLALRFYISFHAVVPAPRTILKGVRKLPPATLRVVSADGSSFDEVYWRLDFRALPGEDGRSAKD
WEDGLFEAVRAAVARRIIADVPVGVLLSGGLDSSLIVGLLAEAGQRDVETFSIGFEEANGEKGDEFFYSDLIAERYATRH
HKIFIPEADMIAALPGAIAAMSEPMVSYDNVAFYLLAREVSKRVKVVLSGQGADEIFGGYHWYRPLVGSTDPVADYAAHF
FDRTPERLARHLSPAWLGTTDEARAFVAAHFAEDGAPDPVDKALRLDSTVMLVEDPVKRVDNMTMAFGLEARVPFLDHQL
AEFAGRIPSSLKLAQNGKGILKEVARRVVPAAVIDREKGYFPVPGLKYIEGSRLDYVRDGLSSRAARQRGLFREDYLASL
YENPRDAITPLRGSELWQVGLLELWLQEHGI

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=548, Percent_Identity=28.4671532846715, Blast_Score=168, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71993933, Length=549, Percent_Identity=26.2295081967213, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25147557, Length=549, Percent_Identity=26.2295081967213, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17560178, Length=515, Percent_Identity=26.6019417475728, Blast_Score=111, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6325403, Length=571, Percent_Identity=25.569176882662, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321563, Length=594, Percent_Identity=24.9158249158249, Blast_Score=123, Evalue=8e-29,
Organism=Drosophila melanogaster, GI45553209, Length=552, Percent_Identity=27.7173913043478, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 64772; Mature: 64772

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVCGEIRFDGQFAGTTAVDRMTAKLAPRGPDDGGILVHGPVALGHRRLSIIDLSPKAA
CCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCHHHCCCEEEEEECCCHHC
QPMVDSDLGLSIVFNGCIYNYPALRSELEALGYRFFSGGDTEVILKAYHAWGTACVKRFN
CCCCCCCCCEEEEECCHHCCCHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHC
GMFAFAIHERDSGKVVLARDRFGIKPLYLAEVDGGLLFASSLPALLATGRVDKAIDPLAL
CEEEEEEEECCCCCEEEEECCCCCCEEEEEECCCCEEEHHCCHHHHHCCCHHHCCCHHEE
RFYISFHAVVPAPRTILKGVRKLPPATLRVVSADGSSFDEVYWRLDFRALPGEDGRSAKD
EEEEEEHEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEEEECCCCCCCCCCC
WEDGLFEAVRAAVARRIIADVPVGVLLSGGLDSSLIVGLLAEAGQRDVETFSIGFEEANG
HHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCC
EKGDEFFYSDLIAERYATRHHKIFIPEADMIAALPGAIAAMSEPMVSYDNVAFYLLAREV
CCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHH
SKRVKVVLSGQGADEIFGGYHWYRPLVGSTDPVADYAAHFFDRTPERLARHLSPAWLGTT
HHHEEEEEECCCCHHHHCCHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCC
DEARAFVAAHFAEDGAPDPVDKALRLDSTVMLVEDPVKRVDNMTMAFGLEARVPFLDHQL
HHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEEECHHHHHHCCCEEEECCCCCCCHHHHHH
AEFAGRIPSSLKLAQNGKGILKEVARRVVPAAVIDREKGYFPVPGLKYIEGSRLDYVRDG
HHHHCCCCCCEEECCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
LSSRAARQRGLFREDYLASLYENPRDAITPLRGSELWQVGLLELWLQEHGI
HHHHHHHHHCCHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCGVCGEIRFDGQFAGTTAVDRMTAKLAPRGPDDGGILVHGPVALGHRRLSIIDLSPKAA
CCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCHHHCCCEEEEEECCCHHC
QPMVDSDLGLSIVFNGCIYNYPALRSELEALGYRFFSGGDTEVILKAYHAWGTACVKRFN
CCCCCCCCCEEEEECCHHCCCHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHC
GMFAFAIHERDSGKVVLARDRFGIKPLYLAEVDGGLLFASSLPALLATGRVDKAIDPLAL
CEEEEEEEECCCCCEEEEECCCCCCEEEEEECCCCEEEHHCCHHHHHCCCHHHCCCHHEE
RFYISFHAVVPAPRTILKGVRKLPPATLRVVSADGSSFDEVYWRLDFRALPGEDGRSAKD
EEEEEEHEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEEEECCCCCCCCCCC
WEDGLFEAVRAAVARRIIADVPVGVLLSGGLDSSLIVGLLAEAGQRDVETFSIGFEEANG
HHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCC
EKGDEFFYSDLIAERYATRHHKIFIPEADMIAALPGAIAAMSEPMVSYDNVAFYLLAREV
CCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHH
SKRVKVVLSGQGADEIFGGYHWYRPLVGSTDPVADYAAHFFDRTPERLARHLSPAWLGTT
HHHEEEEEECCCCHHHHCCHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCC
DEARAFVAAHFAEDGAPDPVDKALRLDSTVMLVEDPVKRVDNMTMAFGLEARVPFLDHQL
HHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEEECHHHHHHCCCEEEECCCCCCCHHHHHH
AEFAGRIPSSLKLAQNGKGILKEVARRVVPAAVIDREKGYFPVPGLKYIEGSRLDYVRDG
HHHHCCCCCCEEECCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
LSSRAARQRGLFREDYLASLYENPRDAITPLRGSELWQVGLLELWLQEHGI
HHHHHHHHHCCHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]