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The map label for this gene is yhfE [H]

Identifier: 83593322

GI number: 83593322

Start: 2296765

End: 2297928

Strand: Reverse

Name: yhfE [H]

Synonym: Rru_A1987

Alternate gene names: 83593322

Gene position: 2297928-2296765 (Counterclockwise)

Preceding gene: 83593323

Following gene: 83593321

Centisome position: 52.79

GC content: 65.64

Gene sequence:

>1164_bases
ATGACGCGTCTGGCGATCGATACGGACTATCTGGCCCGGACTCTGGTTCGCTTGTTAGCCACCCCCAGCCCGACCGGCTA
TACCGATACCGTCGTCCGCGAAACCTGTGCCGAGTTGGAAAGCCTGGGCCTGACCCCGACCCTGACCCGACGGGGGGCGG
TTTGCGTGGTGTTGCGCGGACGGGAAGCCCGGCCGGCCCGCGCCATCGTTTCCCATCTCGACACGCTGGGCGCTCAGGTC
AAGCAGCTCAAAGACAACGGCCGCCTGGAACTGGTGCCGATCGGCACCTGGTCGGCGCGCTTCGCCGAGGGGGCGCGGGT
CACGGTGTTCACCGATCGCGGCGCGGTTCGCGGCACGATTTTGCCGCTGAAGGCCTCGGGCCACATCTTTAACGAAGAGA
TCGACAGCCTGCCGATCGGCTGGCCGATGACCGAGTTGCGGGTCGATGCCCGGGTTCATAGCAAGGCCGATCTGATCGCC
CTGGGTATCGAGGTTGGCGATATCGTCGCCATCGACCCCCAGCCGGAATTCCTAGCCAACGGCTATATCGTGTCCCGCCA
TCTTGATGACAAAGCCGGGGTGGCGCTGATGCTCGCGGCTTTGAAGGCCCTGACCGCCCATAACGAACCGCCGCCCGTCG
ATGTCCATTTCATCTTCACCATCGCCGAGGAAGTCGGCGTCGGCGCGTCTTCGGCGCTGACCGATGACGTCGCCTCGGTG
GTCGCCGTCGATAACGGCACCAGCGGACCCGGCCAGAACTCGGCCGAATTCGGCGTTACCATCGCCATGGCCGACCAGAC
CGGCCCCTTTGATTATCATCTGACCCGGGCGCTGATCCGGCTCTGCCGCGACGAGGACATCATCTTTCGCAAGGATGTGT
TCCGCTACTACCGCTCCGACGCCGCCTCGGCGGTGGTCGCCGGCCACGATGTGCGCAACGCGCTGGTCACCTTTGGCATC
GACGCCTCGCATGGCTATGAGCGCATCCATATGCACGCCCTGCGGTCGGTGGCCGAATTGCTGAGCGCCTATGCGCTGAG
CCCGGTGGAGATCCGCCGAGACGCCGTGGAGACCGCCCGCGGTCTGGCCGGCTTCACCCGCCAGCCACCCCCCGAACCCA
TCGCCGAGGACCTAGCCGTTTCGCAAGAGGGCCCTCTTGCGTGA

Upstream 100 bases:

>100_bases
AAGCCTCGATGATCTAGATGTTTGAGAGGGAAGCAAATCGTCGTCGCGCTCTGATTCCGAGCGCTCGGGATTTGCTTAAA
CGGATGCGAAGGGATTTTTC

Downstream 100 bases:

>100_bases
CGTGGGGCGTCAAGACGCTTTTGTGGGGCGGTGGTATGGGTCCGGCCCCGCTTGAGAGGGAAAAAAGCGGACGTCGATAC
CAAACAAGATTACAAGTTCT

Product: peptidase M42

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MTRLAIDTDYLARTLVRLLATPSPTGYTDTVVRETCAELESLGLTPTLTRRGAVCVVLRGREARPARAIVSHLDTLGAQV
KQLKDNGRLELVPIGTWSARFAEGARVTVFTDRGAVRGTILPLKASGHIFNEEIDSLPIGWPMTELRVDARVHSKADLIA
LGIEVGDIVAIDPQPEFLANGYIVSRHLDDKAGVALMLAALKALTAHNEPPPVDVHFIFTIAEEVGVGASSALTDDVASV
VAVDNGTSGPGQNSAEFGVTIAMADQTGPFDYHLTRALIRLCRDEDIIFRKDVFRYYRSDAASAVVAGHDVRNALVTFGI
DASHGYERIHMHALRSVAELLSAYALSPVEIRRDAVETARGLAGFTRQPPPEPIAEDLAVSQEGPLA

Sequences:

>Translated_387_residues
MTRLAIDTDYLARTLVRLLATPSPTGYTDTVVRETCAELESLGLTPTLTRRGAVCVVLRGREARPARAIVSHLDTLGAQV
KQLKDNGRLELVPIGTWSARFAEGARVTVFTDRGAVRGTILPLKASGHIFNEEIDSLPIGWPMTELRVDARVHSKADLIA
LGIEVGDIVAIDPQPEFLANGYIVSRHLDDKAGVALMLAALKALTAHNEPPPVDVHFIFTIAEEVGVGASSALTDDVASV
VAVDNGTSGPGQNSAEFGVTIAMADQTGPFDYHLTRALIRLCRDEDIIFRKDVFRYYRSDAASAVVAGHDVRNALVTFGI
DASHGYERIHMHALRSVAELLSAYALSPVEIRRDAVETARGLAGFTRQPPPEPIAEDLAVSQEGPLA
>Mature_386_residues
TRLAIDTDYLARTLVRLLATPSPTGYTDTVVRETCAELESLGLTPTLTRRGAVCVVLRGREARPARAIVSHLDTLGAQVK
QLKDNGRLELVPIGTWSARFAEGARVTVFTDRGAVRGTILPLKASGHIFNEEIDSLPIGWPMTELRVDARVHSKADLIAL
GIEVGDIVAIDPQPEFLANGYIVSRHLDDKAGVALMLAALKALTAHNEPPPVDVHFIFTIAEEVGVGASSALTDDVASVV
AVDNGTSGPGQNSAEFGVTIAMADQTGPFDYHLTRALIRLCRDEDIIFRKDVFRYYRSDAASAVVAGHDVRNALVTFGID
ASHGYERIHMHALRSVAELLSAYALSPVEIRRDAVETARGLAGFTRQPPPEPIAEDLAVSQEGPLA

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=234, Percent_Identity=24.7863247863248, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: NA

Molecular weight: Translated: 41639; Mature: 41508

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLAIDTDYLARTLVRLLATPSPTGYTDTVVRETCAELESLGLTPTLTRRGAVCVVLRG
CCEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEC
REARPARAIVSHLDTLGAQVKQLKDNGRLELVPIGTWSARFAEGARVTVFTDRGAVRGTI
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCEEEEEECCCCCEEEE
LPLKASGHIFNEEIDSLPIGWPMTELRVDARVHSKADLIALGIEVGDIVAIDPQPEFLAN
EEEECCCCEEHHHHCCCCCCCCHHHHEEHHHHCCCCCEEEEEEEECCEEEECCCCCHHHC
GYIVSRHLDDKAGVALMLAALKALTAHNEPPPVDVHFIFTIAEEVGVGASSALTDDVASV
CEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEHHHHCCCCCHHHHHHHHHE
VAVDNGTSGPGQNSAEFGVTIAMADQTGPFDYHLTRALIRLCRDEDIIFRKDVFRYYRSD
EEECCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AASAVVAGHDVRNALVTFGIDASHGYERIHMHALRSVAELLSAYALSPVEIRRDAVETAR
HHHEEHCCCHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GLAGFTRQPPPEPIAEDLAVSQEGPLA
HHCCCCCCCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
TRLAIDTDYLARTLVRLLATPSPTGYTDTVVRETCAELESLGLTPTLTRRGAVCVVLRG
CEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEC
REARPARAIVSHLDTLGAQVKQLKDNGRLELVPIGTWSARFAEGARVTVFTDRGAVRGTI
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCEEEEEECCCCCEEEE
LPLKASGHIFNEEIDSLPIGWPMTELRVDARVHSKADLIALGIEVGDIVAIDPQPEFLAN
EEEECCCCEEHHHHCCCCCCCCHHHHEEHHHHCCCCCEEEEEEEECCEEEECCCCCHHHC
GYIVSRHLDDKAGVALMLAALKALTAHNEPPPVDVHFIFTIAEEVGVGASSALTDDVASV
CEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEHHHHCCCCCHHHHHHHHHE
VAVDNGTSGPGQNSAEFGVTIAMADQTGPFDYHLTRALIRLCRDEDIIFRKDVFRYYRSD
EEECCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AASAVVAGHDVRNALVTFGIDASHGYERIHMHALRSVAELLSAYALSPVEIRRDAVETAR
HHHEEHCCCHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GLAGFTRQPPPEPIAEDLAVSQEGPLA
HHCCCCCCCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]