Click here to switch to the map view.

The map label for this gene is thlA [H]

Identifier: 83593281

GI number: 83593281

Start: 2254521

End: 2255741

Strand: Reverse

Name: thlA [H]

Synonym: Rru_A1946

Alternate gene names: 83593281

Gene position: 2255741-2254521 (Counterclockwise)

Preceding gene: 83593282

Following gene: 83593280

Centisome position: 51.82

GC content: 69.86

Gene sequence:

>1221_bases
ATGTCGTCTGCCGCCCCCAGCGCCTTCCCGTTTTCCGCCGGTGATCCGGTGGTGATTGTCGGCGCCAAACGCACGCCGCT
TGGCGCCTTCATGGGATCGCTGGGGTCCTTGCGCGCGCCCGAACTGGGGGCGAGGGCGATCAGCGGCGCGCTGGACGGGC
TGGAGGGGGCGGAGCATCGCGTTGACGGGGTGATCATGGGCTGCGTTCTGCCCGCCGGCCAGGGGCAGGCGCCGGCCCGT
CAGGCCGCCCATGCGGCGGGGCTGTCGTGGGCGGTGCCCTGCGCCACGGTCAACAAGATGTGCGGGTCGGGGATGGAGGC
GGCGATCCTGGCTTGCAATACCCTGGCCGCCGGCGCCGCCGAGGTGATGGTGGCCGGCGGCATGGAAAGCATGACCAACG
CCCCCTATCTTCTGGACAAGGCACGCGGCGGCCATCGGTTGGGCCATGGTCAGGTGGTCGATCACATGTTCCTTGATGGA
CTGGAAGACGCCTATGACCGGGGCCGGCTGATGGGAACCTTCGCCGAGGATACGGCGCAAAGCTATCGGTTCAGTCGCGA
GGAGCAGGACGCCTTCGCCCTCGAGTCGCTGTTGCGGGCCAAGCGCGCCATCGCCGACGGCGTGATGGCGCGGGAATGCG
TGAGCGTGGTCGTGCGCGAGCGCGGCGAGGATCATCTGGTCGAGACCGACGAGGCGCCGATCAAGGCCAATCCCGACCGC
ATCCCCCACTTGAAGCCGGCCTTCCGCGAGGGCGGGACGGTGACGGCGGCCAATAGCTCGTCGATCTCGGATGGGGCGGC
GGCGCTGGTGCTGACCCGGGCCTCGGTGGCCGACCGGGCGGGATGGAGGCCCTTGGCCGTGGTGCGCGGCCATGCGGCCT
ATGCGGCCGAACCCGAATTGTTTCCAACCGCGCCGGTCGGGGCGATCGAACGGATGCTGGCGCGGGTGGGCTGGCGGCGC
GACGAGGTGGATTTGTGGGAGATCAACGAAGCCTTCGCCGTTGTCGCCCTGGTGGCGCTGCGCGACCTCGGTCTCGACCC
CGGGCGTTTGAACGTTCATGGTGGAGCCTGCGCCCTGGGCCATCCGATCGGCGCCTCGGGCGCGCGGATCATCGTCACCT
TGCTCAACGCCCTGCGCCAGCGGGGTTTGACGCGGGGGGTGGCCTCGCTGTGCATCGGTGGCGGCGAGGCGACGGCGATG
GCCGTGGAACTTGCGTCATAG

Upstream 100 bases:

>100_bases
ATGACCACCCGCCACTTGCCGAGCTTGACGGGCTTTATCGCAGCCTTTTCGCAGCGCCCGCCCGCCGCTGAGCCGCGTTT
TTCTGACGCAAGGAGCCGTC

Downstream 100 bases:

>100_bases
CGCTTTGGGCGGAAAAAGCGACCGTCGGCGTTCCCCGCCCGCAGCGCGCCGCCGCCTCCCCATCGGTCCGCTTCTCAAGG
AGACGCCCATGTCGACCATT

Product: acetyl-CoA C-acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSSAAPSAFPFSAGDPVVIVGAKRTPLGAFMGSLGSLRAPELGARAISGALDGLEGAEHRVDGVIMGCVLPAGQGQAPAR
QAAHAAGLSWAVPCATVNKMCGSGMEAAILACNTLAAGAAEVMVAGGMESMTNAPYLLDKARGGHRLGHGQVVDHMFLDG
LEDAYDRGRLMGTFAEDTAQSYRFSREEQDAFALESLLRAKRAIADGVMARECVSVVVRERGEDHLVETDEAPIKANPDR
IPHLKPAFREGGTVTAANSSSISDGAAALVLTRASVADRAGWRPLAVVRGHAAYAAEPELFPTAPVGAIERMLARVGWRR
DEVDLWEINEAFAVVALVALRDLGLDPGRLNVHGGACALGHPIGASGARIIVTLLNALRQRGLTRGVASLCIGGGEATAM
AVELAS

Sequences:

>Translated_406_residues
MSSAAPSAFPFSAGDPVVIVGAKRTPLGAFMGSLGSLRAPELGARAISGALDGLEGAEHRVDGVIMGCVLPAGQGQAPAR
QAAHAAGLSWAVPCATVNKMCGSGMEAAILACNTLAAGAAEVMVAGGMESMTNAPYLLDKARGGHRLGHGQVVDHMFLDG
LEDAYDRGRLMGTFAEDTAQSYRFSREEQDAFALESLLRAKRAIADGVMARECVSVVVRERGEDHLVETDEAPIKANPDR
IPHLKPAFREGGTVTAANSSSISDGAAALVLTRASVADRAGWRPLAVVRGHAAYAAEPELFPTAPVGAIERMLARVGWRR
DEVDLWEINEAFAVVALVALRDLGLDPGRLNVHGGACALGHPIGASGARIIVTLLNALRQRGLTRGVASLCIGGGEATAM
AVELAS
>Mature_405_residues
SSAAPSAFPFSAGDPVVIVGAKRTPLGAFMGSLGSLRAPELGARAISGALDGLEGAEHRVDGVIMGCVLPAGQGQAPARQ
AAHAAGLSWAVPCATVNKMCGSGMEAAILACNTLAAGAAEVMVAGGMESMTNAPYLLDKARGGHRLGHGQVVDHMFLDGL
EDAYDRGRLMGTFAEDTAQSYRFSREEQDAFALESLLRAKRAIADGVMARECVSVVVRERGEDHLVETDEAPIKANPDRI
PHLKPAFREGGTVTAANSSSISDGAAALVLTRASVADRAGWRPLAVVRGHAAYAAEPELFPTAPVGAIERMLARVGWRRD
EVDLWEINEAFAVVALVALRDLGLDPGRLNVHGGACALGHPIGASGARIIVTLLNALRQRGLTRGVASLCIGGGEATAMA
VELAS

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4557237, Length=389, Percent_Identity=45.5012853470437, Blast_Score=322, Evalue=5e-88,
Organism=Homo sapiens, GI148539872, Length=392, Percent_Identity=43.3673469387755, Blast_Score=295, Evalue=4e-80,
Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=35.73264781491, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI4501853, Length=413, Percent_Identity=34.3825665859564, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI4504327, Length=421, Percent_Identity=30.8788598574822, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI194353979, Length=407, Percent_Identity=27.7641277641278, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1788554, Length=389, Percent_Identity=48.8431876606684, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI87082165, Length=388, Percent_Identity=44.3298969072165, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1787663, Length=391, Percent_Identity=36.5728900255754, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI48994986, Length=406, Percent_Identity=35.4679802955665, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1788683, Length=403, Percent_Identity=29.5285359801489, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25147385, Length=385, Percent_Identity=39.7402597402597, Blast_Score=280, Evalue=7e-76,
Organism=Caenorhabditis elegans, GI17535921, Length=389, Percent_Identity=39.3316195372751, Blast_Score=250, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI133906874, Length=392, Percent_Identity=37.7551020408163, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17535917, Length=388, Percent_Identity=32.7319587628866, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17551802, Length=421, Percent_Identity=27.790973871734, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6325229, Length=393, Percent_Identity=45.8015267175573, Blast_Score=313, Evalue=4e-86,
Organism=Saccharomyces cerevisiae, GI6322031, Length=386, Percent_Identity=31.0880829015544, Blast_Score=174, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24655093, Length=391, Percent_Identity=46.0358056265985, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24640423, Length=389, Percent_Identity=44.987146529563, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI17648125, Length=392, Percent_Identity=33.9285714285714, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI17137578, Length=421, Percent_Identity=30.4038004750594, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 42037; Mature: 41906

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAAPSAFPFSAGDPVVIVGAKRTPLGAFMGSLGSLRAPELGARAISGALDGLEGAEHR
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHH
VDGVIMGCVLPAGQGQAPARQAAHAAGLSWAVPCATVNKMCGSGMEAAILACNTLAAGAA
HCEEEEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCHH
EVMVAGGMESMTNAPYLLDKARGGHRLGHGQVVDHMFLDGLEDAYDRGRLMGTFAEDTAQ
HHHEECCHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHH
SYRFSREEQDAFALESLLRAKRAIADGVMARECVSVVVRERGEDHLVETDEAPIKANPDR
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCC
IPHLKPAFREGGTVTAANSSSISDGAAALVLTRASVADRAGWRPLAVVRGHAAYAAEPEL
CCCCCHHHHCCCEEEECCCCCCCCCCEEEEEEHHHHHHHCCCCCEEEEECCCCCCCCCCC
FPTAPVGAIERMLARVGWRRDEVDLWEINEAFAVVALVALRDLGLDPGRLNVHGGACALG
CCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEECC
HPIGASGARIIVTLLNALRQRGLTRGVASLCIGGGEATAMAVELAS
CCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEECC
>Mature Secondary Structure 
SSAAPSAFPFSAGDPVVIVGAKRTPLGAFMGSLGSLRAPELGARAISGALDGLEGAEHR
CCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHH
VDGVIMGCVLPAGQGQAPARQAAHAAGLSWAVPCATVNKMCGSGMEAAILACNTLAAGAA
HCEEEEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCHH
EVMVAGGMESMTNAPYLLDKARGGHRLGHGQVVDHMFLDGLEDAYDRGRLMGTFAEDTAQ
HHHEECCHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHH
SYRFSREEQDAFALESLLRAKRAIADGVMARECVSVVVRERGEDHLVETDEAPIKANPDR
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCC
IPHLKPAFREGGTVTAANSSSISDGAAALVLTRASVADRAGWRPLAVVRGHAAYAAEPEL
CCCCCHHHHCCCEEEECCCCCCCCCCEEEEEEHHHHHHHCCCCCEEEEECCCCCCCCCCC
FPTAPVGAIERMLARVGWRRDEVDLWEINEAFAVVALVALRDLGLDPGRLNVHGGACALG
CCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEECC
HPIGASGARIIVTLLNALRQRGLTRGVASLCIGGGEATAMAVELAS
CCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]