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The map label for this gene is rluE [H]

Identifier: 83593274

GI number: 83593274

Start: 2246881

End: 2247480

Strand: Direct

Name: rluE [H]

Synonym: Rru_A1939

Alternate gene names: 83593274

Gene position: 2246881-2247480 (Clockwise)

Preceding gene: 83593273

Following gene: 83593284

Centisome position: 51.62

GC content: 70.33

Gene sequence:

>600_bases
ATGCCCCGGCTGATCCTTCTCAATAAGCCCTATGACGTGCTCAGTCAGTTCACTGACCGGGCCGAGGGCCGGGCCACCCT
CGCCTCGCTGGTGCCGATCCCCGGGGTTTATGTCTGCGGGCGCCTCGATCGCGATAGCGAAGGCTTGCTGCTGCTGACCG
ACAACGGCCCCTTGCAGCACCACATCAGCCATCCGCGCTCCAAGGTGCCCAAGACCTATCGGGCCCAGGTCGAAGGGGTC
GCCGACAAGGCCGCGCTTGATCGCTTGCGCCAGGGGGTGACGCTGCGCGACGGCCCGGCCCGCGCCGTGACGGTCGAGGC
GATCGACGAGCCGGCCGGGCTGTGGCCGCGCGATCCACCGATCCGCTGGCGGGCGAACATCCCGACGAGTTGGATCGAGC
TGACCATCGACGAGGGCCGCAATCGCATGGTCCGGCGGATGACGGCGGCGGTCGGCCTGCCAACCCTGCGGCTGATCCGC
TGGTCGATCGGCGATTGGACCTTGGAGAACCTGCCGCCGGGAAGCTGGCGCGAGGAGACCGCGAGCCTGCCGGCGCGTCG
CCGCCCCGCCGCCAAACCGCCGCGCCCTCCCCGCCGTTAG

Upstream 100 bases:

>100_bases
CCGCCCTGCCCGAGGTGCTGCCCTGGCTGGCCGGGGTGCTGGCCGAGCCCGGCGGCGGCGACTGGCGTCTTGACTGGTTG
ATCGAGGAGAGGATGGGACC

Downstream 100 bases:

>100_bases
CCGCCACGCCCTTTCCTCGCTGCCAGCGCGCGGGCGGCGCGCGAGGCCGGGGGGCGCCCCAGGGTCTGGCAAAGCGCCAG
ACCGGCGAGGCGCAGCTTCT

Product: ribosomal large subunit pseudouridine synthase E

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MPRLILLNKPYDVLSQFTDRAEGRATLASLVPIPGVYVCGRLDRDSEGLLLLTDNGPLQHHISHPRSKVPKTYRAQVEGV
ADKAALDRLRQGVTLRDGPARAVTVEAIDEPAGLWPRDPPIRWRANIPTSWIELTIDEGRNRMVRRMTAAVGLPTLRLIR
WSIGDWTLENLPPGSWREETASLPARRRPAAKPPRPPRR

Sequences:

>Translated_199_residues
MPRLILLNKPYDVLSQFTDRAEGRATLASLVPIPGVYVCGRLDRDSEGLLLLTDNGPLQHHISHPRSKVPKTYRAQVEGV
ADKAALDRLRQGVTLRDGPARAVTVEAIDEPAGLWPRDPPIRWRANIPTSWIELTIDEGRNRMVRRMTAAVGLPTLRLIR
WSIGDWTLENLPPGSWREETASLPARRRPAAKPPRPPRR
>Mature_198_residues
PRLILLNKPYDVLSQFTDRAEGRATLASLVPIPGVYVCGRLDRDSEGLLLLTDNGPLQHHISHPRSKVPKTYRAQVEGVA
DKAALDRLRQGVTLRDGPARAVTVEAIDEPAGLWPRDPPIRWRANIPTSWIELTIDEGRNRMVRRMTAAVGLPTLRLIRW
SIGDWTLENLPPGSWREETASLPARRRPAAKPPRPPRR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=60.6741573033708, Blast_Score=222, Evalue=1e-59,
Organism=Escherichia coli, GI1787524, Length=182, Percent_Identity=36.2637362637363, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI1788510, Length=190, Percent_Identity=35.7894736842105, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 22372; Mature: 22241

Theoretical pI: Translated: 11.26; Mature: 11.26

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLILLNKPYDVLSQFTDRAEGRATLASLVPIPGVYVCGRLDRDSEGLLLLTDNGPLQH
CCCEEEEECCHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCCHH
HISHPRSKVPKTYRAQVEGVADKAALDRLRQGVTLRDGPARAVTVEAIDEPAGLWPRDPP
HHCCCHHHCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
IRWRANIPTSWIELTIDEGRNRMVRRMTAAVGLPTLRLIRWSIGDWTLENLPPGSWREET
CEEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHHEEECCCCEEECCCCCCCCCHHH
ASLPARRRPAAKPPRPPRR
HCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PRLILLNKPYDVLSQFTDRAEGRATLASLVPIPGVYVCGRLDRDSEGLLLLTDNGPLQH
CCEEEEECCHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCCHH
HISHPRSKVPKTYRAQVEGVADKAALDRLRQGVTLRDGPARAVTVEAIDEPAGLWPRDPP
HHCCCHHHCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
IRWRANIPTSWIELTIDEGRNRMVRRMTAAVGLPTLRLIRWSIGDWTLENLPPGSWREET
CEEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHHEEECCCCEEECCCCCCCCCHHH
ASLPARRRPAAKPPRPPRR
HCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]