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The map label for this gene is pleD [H]

Identifier: 83593243

GI number: 83593243

Start: 2211932

End: 2213491

Strand: Reverse

Name: pleD [H]

Synonym: Rru_A1908

Alternate gene names: 83593243

Gene position: 2213491-2211932 (Counterclockwise)

Preceding gene: 83593246

Following gene: 83593240

Centisome position: 50.85

GC content: 62.69

Gene sequence:

>1560_bases
ATGGGGCGGAAACAGCTAAATCATATAAAATGTAGAGAAAATTGTTCGCTTGTCGCCAAGGCCGGATTTCGGCACCGTAG
GGTCATAATCCAGGGGATACAGTCAGCTATGGTGCAAGACAGCGACGAGATCTTGTTTGTCGAGGAAGAGGATCCCGTCG
CAGGTTCCTTGCCTTCCGATGTGTCGCCCTGGAAGGTTTTGGTCGCCGATGACGATCCCGAAGTCTTCACGGTGACCCGG
CTGGTGCTGTCGGGATTTGTTTTCGCCGGGCGTGGCGTATCCTTGCTTTACGCCGGTTCGGCGGCGGAAGCGACTGAAAT
ACTCAGGCAACAGTCCGATATCGCCGTCGCCCTGCTTGATGTGGTGATGGAAACCGAGGACGCCGGCCTAAAGCTGGTCA
AGGTTATCCGCGATGACCTGGGCAACAGTCATTTGCGGATTGTCTTGCGAACCGGCCAGCCCGGGCAGGCGCCCGAACAC
GACGTGGTTATGCGCTATGACATCAACGATTATCGCTCCAAGACCGAACTGACCGATACCAAGCTGATCACCACCCTGGT
GTCGTCCCTGCGCTCCTATCAGCAGATCGAAGCCCTTGAACGCAGCCGCGCCGAACTGCGCGAAGCCCGTGATACCCTGG
AGATCCGCGTCGCCGAACGCACCCGCGACCTGCGCGAAAGCGAGGCCCGGCTGCGCTCGATCCTCGACGCCTCGGTTTTT
CCGATCCTGGTGCTGAGCGAGGAGGGCGACCGGGTACGCTTTCTCAACGATCACGCCGGCGAGGCCCTGGGCTGGGCGCT
TCTTGAGAACACCACGTCGACGCTGCCCGACCTGTGGGAAAACCCCGTCGATGGCGCCCGTTTGCTGGCGCGCACGGTCC
AGCGCGGTCGGGTGCTCGACTGCGAATTCCGCTTGCGCGGGGCGGGCGGCGAGCCGTTCTGGGCCCTGGTCTCGACCATC
GCCATGTCCTATGACGGGCAGCCGGCGGTCTTGCTGTCGTTTAATGACATCACCGTTCACAAGCAGCGCGAGGAAGCCTG
GCAGCGCTTGGCCCTGACCGACCCGCTGACCGGCCTGGGCAATCGCCGGCTGCTGTTCGATCGCGGCATCCTCGAGGTCG
CCCGGGCGCGTCGCGGTTGTTGGCCGCTTTCGGGGCTGATGATCGATCTTGATCACTTCAAGCGGATCAATGACACCCAG
GGCCATGCGGCGGGTGATGCGATCTTGCGCACCCTGGCCGATACCCTGCGCGAAAGCCTGCGCGAGATCGATGTCATCGC
CCGCGTCGGCGGCGAGGAATTCGCCATTCTGCTGCCCGACACCTCCTTGACGGCGGCCCATGAGGTCGGCGAAAGGCTGC
GGCTGTCGTGTCAGATCCCCCAGCCCGTCGGCGGTCCGGTGACGATCAGCGTCGGCATCTCCACCCTGCATCCCGAGGAT
CGTGGGCTGGGGGATATGTTGTCGCGCGCCGACGATGCGCTCTATCGGGCCAAGCGCAACGGCCGCGACCGGGTGGAAAT
CGCCGATGACTCTGAAGACAATCAGGACGTCAGCCCCTGA

Upstream 100 bases:

>100_bases
GGCAGGGTACCCTTTCCCCGATATGGGGCGGCAAAAATGGCCCAAAAAGGGCCAAGCGGCGCAGGGTCTTTAACCCCCTC
CCGAGGAACGGGGGTTGGAT

Downstream 100 bases:

>100_bases
AAGGGGGACCGACGCCCTGGGAGAGAGCCGCTTTTTTTGCGCCCTAGTCGTCGGTCGGTGTGTGCGAAGCCGTCGCCTGG
GGGGCTTCCGGCTTGAAGTC

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MGRKQLNHIKCRENCSLVAKAGFRHRRVIIQGIQSAMVQDSDEILFVEEEDPVAGSLPSDVSPWKVLVADDDPEVFTVTR
LVLSGFVFAGRGVSLLYAGSAAEATEILRQQSDIAVALLDVVMETEDAGLKLVKVIRDDLGNSHLRIVLRTGQPGQAPEH
DVVMRYDINDYRSKTELTDTKLITTLVSSLRSYQQIEALERSRAELREARDTLEIRVAERTRDLRESEARLRSILDASVF
PILVLSEEGDRVRFLNDHAGEALGWALLENTTSTLPDLWENPVDGARLLARTVQRGRVLDCEFRLRGAGGEPFWALVSTI
AMSYDGQPAVLLSFNDITVHKQREEAWQRLALTDPLTGLGNRRLLFDRGILEVARARRGCWPLSGLMIDLDHFKRINDTQ
GHAAGDAILRTLADTLRESLREIDVIARVGGEEFAILLPDTSLTAAHEVGERLRLSCQIPQPVGGPVTISVGISTLHPED
RGLGDMLSRADDALYRAKRNGRDRVEIADDSEDNQDVSP

Sequences:

>Translated_519_residues
MGRKQLNHIKCRENCSLVAKAGFRHRRVIIQGIQSAMVQDSDEILFVEEEDPVAGSLPSDVSPWKVLVADDDPEVFTVTR
LVLSGFVFAGRGVSLLYAGSAAEATEILRQQSDIAVALLDVVMETEDAGLKLVKVIRDDLGNSHLRIVLRTGQPGQAPEH
DVVMRYDINDYRSKTELTDTKLITTLVSSLRSYQQIEALERSRAELREARDTLEIRVAERTRDLRESEARLRSILDASVF
PILVLSEEGDRVRFLNDHAGEALGWALLENTTSTLPDLWENPVDGARLLARTVQRGRVLDCEFRLRGAGGEPFWALVSTI
AMSYDGQPAVLLSFNDITVHKQREEAWQRLALTDPLTGLGNRRLLFDRGILEVARARRGCWPLSGLMIDLDHFKRINDTQ
GHAAGDAILRTLADTLRESLREIDVIARVGGEEFAILLPDTSLTAAHEVGERLRLSCQIPQPVGGPVTISVGISTLHPED
RGLGDMLSRADDALYRAKRNGRDRVEIADDSEDNQDVSP
>Mature_518_residues
GRKQLNHIKCRENCSLVAKAGFRHRRVIIQGIQSAMVQDSDEILFVEEEDPVAGSLPSDVSPWKVLVADDDPEVFTVTRL
VLSGFVFAGRGVSLLYAGSAAEATEILRQQSDIAVALLDVVMETEDAGLKLVKVIRDDLGNSHLRIVLRTGQPGQAPEHD
VVMRYDINDYRSKTELTDTKLITTLVSSLRSYQQIEALERSRAELREARDTLEIRVAERTRDLRESEARLRSILDASVFP
ILVLSEEGDRVRFLNDHAGEALGWALLENTTSTLPDLWENPVDGARLLARTVQRGRVLDCEFRLRGAGGEPFWALVSTIA
MSYDGQPAVLLSFNDITVHKQREEAWQRLALTDPLTGLGNRRLLFDRGILEVARARRGCWPLSGLMIDLDHFKRINDTQG
HAAGDAILRTLADTLRESLREIDVIARVGGEEFAILLPDTSLTAAHEVGERLRLSCQIPQPVGGPVTISVGISTLHPEDR
GLGDMLSRADDALYRAKRNGRDRVEIADDSEDNQDVSP

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=183, Percent_Identity=39.8907103825137, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787262, Length=172, Percent_Identity=35.4651162790698, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI87082007, Length=171, Percent_Identity=40.9356725146199, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI145693134, Length=164, Percent_Identity=39.6341463414634, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=38.728323699422, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788381, Length=313, Percent_Identity=28.4345047923323, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787541, Length=216, Percent_Identity=31.4814814814815, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1787816, Length=171, Percent_Identity=35.672514619883, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788085, Length=172, Percent_Identity=29.6511627906977, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87081974, Length=194, Percent_Identity=30.4123711340206, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1787802, Length=131, Percent_Identity=31.2977099236641, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI87081977, Length=162, Percent_Identity=30.8641975308642, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788956, Length=167, Percent_Identity=32.9341317365269, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 57643; Mature: 57512

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRKQLNHIKCRENCSLVAKAGFRHRRVIIQGIQSAMVQDSDEILFVEEEDPVAGSLPSD
CCCCCCCCHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC
VSPWKVLVADDDPEVFTVTRLVLSGFVFAGRGVSLLYAGSAAEATEILRQQSDIAVALLD
CCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCHHHHHHHH
VVMETEDAGLKLVKVIRDDLGNSHLRIVLRTGQPGQAPEHDVVMRYDINDYRSKTELTDT
HHHCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEECCHHHHCCCHHHHH
KLITTLVSSLRSYQQIEALERSRAELREARDTLEIRVAERTRDLRESEARLRSILDASVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHCCCC
PILVLSEEGDRVRFLNDHAGEALGWALLENTTSTLPDLWENPVDGARLLARTVQRGRVLD
EEEEECCCCCEEEEECCCCCHHHHHHHHHCCHHCCHHHHCCCCHHHHHHHHHHHCCCEEE
CEFRLRGAGGEPFWALVSTIAMSYDGQPAVLLSFNDITVHKQREEAWQRLALTDPLTGLG
EEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCEEEECHHHHHHHHHHHCCCCCCCC
NRRLLFDRGILEVARARRGCWPLSGLMIDLDHFKRINDTQGHAAGDAILRTLADTLRESL
CCEEEEHHHHHHHHHHHCCCCCCCCEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
REIDVIARVGGEEFAILLPDTSLTAAHEVGERLRLSCQIPQPVGGPVTISVGISTLHPED
HHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHEEEEEECCCCCCCCEEEEEEEEEECCCC
RGLGDMLSRADDALYRAKRNGRDRVEIADDSEDNQDVSP
CCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
GRKQLNHIKCRENCSLVAKAGFRHRRVIIQGIQSAMVQDSDEILFVEEEDPVAGSLPSD
CCCCCCCHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC
VSPWKVLVADDDPEVFTVTRLVLSGFVFAGRGVSLLYAGSAAEATEILRQQSDIAVALLD
CCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCHHHHHHHH
VVMETEDAGLKLVKVIRDDLGNSHLRIVLRTGQPGQAPEHDVVMRYDINDYRSKTELTDT
HHHCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEECCHHHHCCCHHHHH
KLITTLVSSLRSYQQIEALERSRAELREARDTLEIRVAERTRDLRESEARLRSILDASVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHCCCC
PILVLSEEGDRVRFLNDHAGEALGWALLENTTSTLPDLWENPVDGARLLARTVQRGRVLD
EEEEECCCCCEEEEECCCCCHHHHHHHHHCCHHCCHHHHCCCCHHHHHHHHHHHCCCEEE
CEFRLRGAGGEPFWALVSTIAMSYDGQPAVLLSFNDITVHKQREEAWQRLALTDPLTGLG
EEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCEEEECHHHHHHHHHHHCCCCCCCC
NRRLLFDRGILEVARARRGCWPLSGLMIDLDHFKRINDTQGHAAGDAILRTLADTLRESL
CCEEEEHHHHHHHHHHHCCCCCCCCEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
REIDVIARVGGEEFAILLPDTSLTAAHEVGERLRLSCQIPQPVGGPVTISVGISTLHPED
HHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHEEEEEECCCCCCCCEEEEEEEEEECCCC
RGLGDMLSRADDALYRAKRNGRDRVEIADDSEDNQDVSP
CCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA