Click here to switch to the map view.

The map label for this gene is pdxJ

Identifier: 83593189

GI number: 83593189

Start: 2152562

End: 2153311

Strand: Reverse

Name: pdxJ

Synonym: Rru_A1854

Alternate gene names: 83593189

Gene position: 2153311-2152562 (Counterclockwise)

Preceding gene: 83593190

Following gene: 83593188

Centisome position: 49.47

GC content: 69.87

Gene sequence:

>750_bases
ATGAGCCGGGTGTTGAGGCTGGGTGTGAACATCGACCATGTGGCGACGATTCGCAACGCCAGGGGCGGGGACCATCCCGA
CCCGCTGCGCGCGGCCAAGATCGCAGCAGAGGCCGGGGCCGATGGCATCACCGCCCATCTGCGCGAGGATCGCCGCCATA
TCGGCGATCGCGATATCGCCCGCCTGCGTGACGAGATCGATCTGCCGCTGAACCTGGAAATGGCCGCGACCGCCGAGATG
CTGGCCATCGCCCTGCGCCATCGCCCCCATGCCGCCTGCATCGTGCCCGAAAAGCGCGAGGAGCGGACGACCGAGGGCGG
GCTGGACGTTGTTGGCGGGGCCGTCCATCTGGCGCCGATCATCACAGCCCTGGGCGAGGCCGGGGTGCGGGTGTCGCTGT
TTATCGAACCCGATCTCCGCCAGCTCGACGCCGCCCGCGCCTTGGGCGCTCCGGTGGTGGAACTCCATACCGGCGCCTAT
TGCGACGCCGCCGCCGGTCCGGCGCGCGAGGCCCAGTTCCTGCGCATCGACAAGGCGGCCCGCCATGCCCAGGCCCTGGG
GCTGGAATGCCATGCCGGCCACGGTCTGACCTATGACACGGTCGAGCCGGTGGCGGCGCTGCCCGCCATCGCCGAGCTCA
ATATCGGCCATTTCCTGATCGGCGAGGCGATCTTCGTCGGCTTGCATCAGGCGATCGCCCGCATGCGCGCCCATATGGAC
GCGGCGCTGCGCGGCGGGGTGGCGGCGTAA

Upstream 100 bases:

>100_bases
AGGCGCGGATCCGCGAGGAGAAGTTGGCCGCCGCCGAAGGCGGCGTGCCGCTGTCGCGACAGCAACCCTGATCCCCCTTG
GGCCCCTTAAGGAGAGAACG

Downstream 100 bases:

>100_bases
GCGGCGCGCTCCGCTGGCGGGGGGACCCCATGCCAGCCTTGACATTGGCCGGGCGCGGGGGCTTCATCTGGCGGCCGGAC
TCCGTCGAGGAGGCCGCGTT

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSRVLRLGVNIDHVATIRNARGGDHPDPLRAAKIAAEAGADGITAHLREDRRHIGDRDIARLRDEIDLPLNLEMAATAEM
LAIALRHRPHAACIVPEKREERTTEGGLDVVGGAVHLAPIITALGEAGVRVSLFIEPDLRQLDAARALGAPVVELHTGAY
CDAAAGPAREAQFLRIDKAARHAQALGLECHAGHGLTYDTVEPVAALPAIAELNIGHFLIGEAIFVGLHQAIARMRAHMD
AALRGGVAA

Sequences:

>Translated_249_residues
MSRVLRLGVNIDHVATIRNARGGDHPDPLRAAKIAAEAGADGITAHLREDRRHIGDRDIARLRDEIDLPLNLEMAATAEM
LAIALRHRPHAACIVPEKREERTTEGGLDVVGGAVHLAPIITALGEAGVRVSLFIEPDLRQLDAARALGAPVVELHTGAY
CDAAAGPAREAQFLRIDKAARHAQALGLECHAGHGLTYDTVEPVAALPAIAELNIGHFLIGEAIFVGLHQAIARMRAHMD
AALRGGVAA
>Mature_248_residues
SRVLRLGVNIDHVATIRNARGGDHPDPLRAAKIAAEAGADGITAHLREDRRHIGDRDIARLRDEIDLPLNLEMAATAEML
AIALRHRPHAACIVPEKREERTTEGGLDVVGGAVHLAPIITALGEAGVRVSLFIEPDLRQLDAARALGAPVVELHTGAYC
DAAAGPAREAQFLRIDKAARHAQALGLECHAGHGLTYDTVEPVAALPAIAELNIGHFLIGEAIFVGLHQAIARMRAHMDA
ALRGGVAA

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=235, Percent_Identity=55.7446808510638, Blast_Score=258, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_RHORT (Q2RT91)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426941.1
- ProteinModelPortal:   Q2RT91
- SMR:   Q2RT91
- STRING:   Q2RT91
- GeneID:   3835278
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1854
- NMPDR:   fig|1085.1.peg.2428
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- PhylomeDB:   Q2RT91
- ProtClustDB:   PRK05265
- BioCyc:   RRUB269796:RRU_A1854-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 26413; Mature: 26281

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: ACT_SITE 46-46 ACT_SITE 73-73 ACT_SITE 194-194 BINDING 10-10 BINDING 21-21 BINDING 48-48 BINDING 53-53 BINDING 103-103 BINDING 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRVLRLGVNIDHVATIRNARGGDHPDPLRAAKIAAEAGADGITAHLREDRRHIGDRDIA
CCCEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHH
RLRDEIDLPLNLEMAATAEMLAIALRHRPHAACIVPEKREERTTEGGLDVVGGAVHLAPI
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHCCCCCCCHHHCHHHHHHHH
ITALGEAGVRVSLFIEPDLRQLDAARALGAPVVELHTGAYCDAAAGPAREAQFLRIDKAA
HHHHCCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHH
RHAQALGLECHAGHGLTYDTVEPVAALPAIAELNIGHFLIGEAIFVGLHQAIARMRAHMD
HHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AALRGGVAA
HHHCCCCCC
>Mature Secondary Structure 
SRVLRLGVNIDHVATIRNARGGDHPDPLRAAKIAAEAGADGITAHLREDRRHIGDRDIA
CCEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHH
RLRDEIDLPLNLEMAATAEMLAIALRHRPHAACIVPEKREERTTEGGLDVVGGAVHLAPI
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHCCCCCCCHHHCHHHHHHHH
ITALGEAGVRVSLFIEPDLRQLDAARALGAPVVELHTGAYCDAAAGPAREAQFLRIDKAA
HHHHCCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHH
RHAQALGLECHAGHGLTYDTVEPVAALPAIAELNIGHFLIGEAIFVGLHQAIARMRAHMD
HHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AALRGGVAA
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA