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The map label for this gene is xthA [H]

Identifier: 83593119

GI number: 83593119

Start: 2075264

End: 2076064

Strand: Direct

Name: xthA [H]

Synonym: Rru_A1784

Alternate gene names: 83593119

Gene position: 2075264-2076064 (Clockwise)

Preceding gene: 83593116

Following gene: 83593120

Centisome position: 47.68

GC content: 63.8

Gene sequence:

>801_bases
ATGGTGAGTATCGCCACCTTCAACGTCAACAGCGTCAAGGCCCGCCTGCCCAACCTGCTCGACTGGCTGGGAACACGCTC
TCCCGACGTCGCCTTGCTCCAGGAGATCAAATGCCTGGACGAGGCCTTTCCCCGCGCCGAGATCGAGGCCCTGGGCTACC
GACTCGCCGTCCATGGCCAGAAAGCCTATAATGGCGTGGCCATTCTGTCGCGCCTGCCGCTGAGCGAGGTCACCGCCCGC
CTGCCGGGACTCGATGGCGCCCCGGCCGAGGACGATGATCAGGCCCGCTACCTGGAAGCCGTGGTCGATGGCCGCTTCCG
GGTCTGCTGCCTGTATCTGCCCAATGGCAATCCGGTCGAGGAGGACCGCAAGTTCGGCTACAAGCTGGCCTGGATGGATC
GGCTGATCGTCCGCGCGACACATCTTATGCGCGACTTCCCCGATACACCCGTTATACTCGGGGGCGATTTCAACGTCTGT
CCGGACGATCTCGACGTTTATGACCCCGCCGGCTGGGCCAATGACGCCCTGTGCCGCCCGGAAACCCGGCAACGCTTCCG
CGCCCTTGTCTTCCTTGGCTATACCGATGCCCTGCGCGCCCTGAACCCCGGGGCGCCGCTTTTCACCTTTTGGGATTATC
AAGCCGGCGCTTGGCAAAAGGATCACGGCCTGCGCATCGATCATCTGCTGCTTAATCCGGCAGCGGCGGACCGTTTGAGC
GCCGCTGGCGTCGATCGCGCCACCCGCGGACACGCTAAGTCGAGCGATCATGCACCGTCTTGGGTGACGCTCGAGCTATA
A

Upstream 100 bases:

>100_bases
GACCGGCACGACCATCCGCAGCGCGCGCCGCCCCACCGTGGTAAGAAGGGCCATCCGCCGGTTTCCTTTGTTCATGGACT
ATGGTTCGGAGTCCGTCCGT

Downstream 100 bases:

>100_bases
CCGGTTAAGATATGTGGATTAGCAACCATGGGGACGTGACCATGACAGAGTTTCCCACTCCTAAAAACCCGACCGCCACC
GTGAAGCCCGCTTCGGAGCA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MVSIATFNVNSVKARLPNLLDWLGTRSPDVALLQEIKCLDEAFPRAEIEALGYRLAVHGQKAYNGVAILSRLPLSEVTAR
LPGLDGAPAEDDDQARYLEAVVDGRFRVCCLYLPNGNPVEEDRKFGYKLAWMDRLIVRATHLMRDFPDTPVILGGDFNVC
PDDLDVYDPAGWANDALCRPETRQRFRALVFLGYTDALRALNPGAPLFTFWDYQAGAWQKDHGLRIDHLLLNPAAADRLS
AAGVDRATRGHAKSSDHAPSWVTLEL

Sequences:

>Translated_266_residues
MVSIATFNVNSVKARLPNLLDWLGTRSPDVALLQEIKCLDEAFPRAEIEALGYRLAVHGQKAYNGVAILSRLPLSEVTAR
LPGLDGAPAEDDDQARYLEAVVDGRFRVCCLYLPNGNPVEEDRKFGYKLAWMDRLIVRATHLMRDFPDTPVILGGDFNVC
PDDLDVYDPAGWANDALCRPETRQRFRALVFLGYTDALRALNPGAPLFTFWDYQAGAWQKDHGLRIDHLLLNPAAADRLS
AAGVDRATRGHAKSSDHAPSWVTLEL
>Mature_266_residues
MVSIATFNVNSVKARLPNLLDWLGTRSPDVALLQEIKCLDEAFPRAEIEALGYRLAVHGQKAYNGVAILSRLPLSEVTAR
LPGLDGAPAEDDDQARYLEAVVDGRFRVCCLYLPNGNPVEEDRKFGYKLAWMDRLIVRATHLMRDFPDTPVILGGDFNVC
PDDLDVYDPAGWANDALCRPETRQRFRALVFLGYTDALRALNPGAPLFTFWDYQAGAWQKDHGLRIDHLLLNPAAADRLS
AAGVDRATRGHAKSSDHAPSWVTLEL

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=274, Percent_Identity=29.1970802919708, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI18375503, Length=274, Percent_Identity=29.1970802919708, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI18375501, Length=274, Percent_Identity=29.1970802919708, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1788046, Length=274, Percent_Identity=33.2116788321168, Blast_Score=127, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI71989536, Length=243, Percent_Identity=29.6296296296296, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI221330655, Length=270, Percent_Identity=28.1481481481481, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI17136678, Length=270, Percent_Identity=28.5185185185185, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29541; Mature: 29541

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSIATFNVNSVKARLPNLLDWLGTRSPDVALLQEIKCLDEAFPRAEIEALGYRLAVHGQ
CEEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHCEEEEECCC
KAYNGVAILSRLPLSEVTARLPGLDGAPAEDDDQARYLEAVVDGRFRVCCLYLPNGNPVE
HHHCHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCC
EDRKFGYKLAWMDRLIVRATHLMRDFPDTPVILGGDFNVCPDDLDVYDPAGWANDALCRP
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
ETRQRFRALVFLGYTDALRALNPGAPLFTFWDYQAGAWQKDHGLRIDHLLLNPAAADRLS
HHHHHHHHHHEECHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEEHEEECCHHHHHHH
AAGVDRATRGHAKSSDHAPSWVTLEL
HCCCCHHHCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MVSIATFNVNSVKARLPNLLDWLGTRSPDVALLQEIKCLDEAFPRAEIEALGYRLAVHGQ
CEEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHCEEEEECCC
KAYNGVAILSRLPLSEVTARLPGLDGAPAEDDDQARYLEAVVDGRFRVCCLYLPNGNPVE
HHHCHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCC
EDRKFGYKLAWMDRLIVRATHLMRDFPDTPVILGGDFNVCPDDLDVYDPAGWANDALCRP
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
ETRQRFRALVFLGYTDALRALNPGAPLFTFWDYQAGAWQKDHGLRIDHLLLNPAAADRLS
HHHHHHHHHHEECHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEEHEEECCHHHHHHH
AAGVDRATRGHAKSSDHAPSWVTLEL
HCCCCHHHCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]