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The map label for this gene is yneJ [H]

Identifier: 83592987

GI number: 83592987

Start: 1942998

End: 1943897

Strand: Reverse

Name: yneJ [H]

Synonym: Rru_A1652

Alternate gene names: 83592987

Gene position: 1943897-1942998 (Counterclockwise)

Preceding gene: 83592990

Following gene: 83592986

Centisome position: 44.66

GC content: 67.56

Gene sequence:

>900_bases
ATGGACCTCGCCGCGCTAGCGGTCTTTCGCACGGTGGCCCGCGAACAGAGCGTCACCCGCGCCGCCGAACTGCTTGGCCG
GGTTCCCTCGAATGTCACGACCCGCGTTCAGCAGCTCGAGGCGGAAATCGGCGTGGCGCTGTTTCAACGCGACAAGAAGC
GCATGGTGTTGACGGCGGCAGGCGAGACCTATCTCGACTATGCCGAGCGCATTCTGAATTTGGCCGAGGAGGCGCAACAG
CGTGTCAAGCCGCTCGCTCCGTTTGGAACCTTGCGCGTCGGCTCAATGGAATGCGCGGTCGCCAGCCGGCTCCCCGCCCC
GCTCGCCCGCTACAGCGCCGCTTGGCCGCAGGTGAGGATCGAGCTGACGACCGCGCCGACCCGTCCCCTGATCGAGGCGG
TTCTGGCGCGGCGGATCGATTGCGCGCTGATCGCCCTGCCCTCGGGCGAGGGGTGGCTCGGGCCTACCCCCCTCGACACG
GTGCCGGTATTCCGCGAGGACTTGGTTTTGCTGCTGCCGCACGGCCATCCCGAAGTCCACACAGCCGAAGATATCAAGCC
CCGAACCCTGGCGACCTTCGCGCCGGGGTGCACCTACCGCGGGCTCGCCGAAGACTGGCTGACCGACCAGGGCCGGCGGC
AGATGGCGTTCGCCATCCAGGAGGTTCGGTCCTACCACGCGATGTTCGCCTGCACGGCGGCGGGATCTTGCGTCAGCGTC
ATGCCGCGCGGCTTGGTCGAGCTCATGGATCATCGGGAAGCGGTCGAGGAAAAGCCGCTGAGGACGGTCGACACGTACCT
CGCCTCCCGCCCCGGCTTCGCGACGTCCGCTTTCGCCGCCTTCCGCGAGACGCTGGGCGAATTCTCGGACTTCAAGGAGG
AAGGCGATGGAGCGGGCTGA

Upstream 100 bases:

>100_bases
CACCGTCATGTGCCGGTTCCCGATCCGGGGATTTCAATGGACAGTTATTCTTTTTTAGAGAATTAATGAACTTTCTAATT
CGCCAATGGAGAATTTATCC

Downstream 100 bases:

>100_bases
CCAAACGGCGACAATCGCCGCGGCCCTGGTGTTGGTCATCGGCATGGGCTTTGGTCGCTTCGCCTTCACCGGTCTGTACC
CGCTGATGGTGGCCGACCAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MDLAALAVFRTVAREQSVTRAAELLGRVPSNVTTRVQQLEAEIGVALFQRDKKRMVLTAAGETYLDYAERILNLAEEAQQ
RVKPLAPFGTLRVGSMECAVASRLPAPLARYSAAWPQVRIELTTAPTRPLIEAVLARRIDCALIALPSGEGWLGPTPLDT
VPVFREDLVLLLPHGHPEVHTAEDIKPRTLATFAPGCTYRGLAEDWLTDQGRRQMAFAIQEVRSYHAMFACTAAGSCVSV
MPRGLVELMDHREAVEEKPLRTVDTYLASRPGFATSAFAAFRETLGEFSDFKEEGDGAG

Sequences:

>Translated_299_residues
MDLAALAVFRTVAREQSVTRAAELLGRVPSNVTTRVQQLEAEIGVALFQRDKKRMVLTAAGETYLDYAERILNLAEEAQQ
RVKPLAPFGTLRVGSMECAVASRLPAPLARYSAAWPQVRIELTTAPTRPLIEAVLARRIDCALIALPSGEGWLGPTPLDT
VPVFREDLVLLLPHGHPEVHTAEDIKPRTLATFAPGCTYRGLAEDWLTDQGRRQMAFAIQEVRSYHAMFACTAAGSCVSV
MPRGLVELMDHREAVEEKPLRTVDTYLASRPGFATSAFAAFRETLGEFSDFKEEGDGAG
>Mature_299_residues
MDLAALAVFRTVAREQSVTRAAELLGRVPSNVTTRVQQLEAEIGVALFQRDKKRMVLTAAGETYLDYAERILNLAEEAQQ
RVKPLAPFGTLRVGSMECAVASRLPAPLARYSAAWPQVRIELTTAPTRPLIEAVLARRIDCALIALPSGEGWLGPTPLDT
VPVFREDLVLLLPHGHPEVHTAEDIKPRTLATFAPGCTYRGLAEDWLTDQGRRQMAFAIQEVRSYHAMFACTAAGSCVSV
MPRGLVELMDHREAVEEKPLRTVDTYLASRPGFATSAFAAFRETLGEFSDFKEEGDGAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=289, Percent_Identity=36.3321799307958, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI87082132, Length=251, Percent_Identity=29.0836653386454, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788887, Length=184, Percent_Identity=32.6086956521739, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787879, Length=253, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788296, Length=223, Percent_Identity=27.8026905829596, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788481, Length=171, Percent_Identity=30.4093567251462, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI145693105, Length=247, Percent_Identity=21.4574898785425, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786401, Length=196, Percent_Identity=28.0612244897959, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI48994962, Length=144, Percent_Identity=31.25, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790208, Length=149, Percent_Identity=30.8724832214765, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32765; Mature: 32765

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLAALAVFRTVAREQSVTRAAELLGRVPSNVTTRVQQLEAEIGVALFQRDKKRMVLTAA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GETYLDYAERILNLAEEAQQRVKPLAPFGTLRVGSMECAVASRLPAPLARYSAAWPQVRI
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHCCCHHHHHHCCCCEEEE
ELTTAPTRPLIEAVLARRIDCALIALPSGEGWLGPTPLDTVPVFREDLVLLLPHGHPEVH
EEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHCCEEEEECCCCCCCC
TAEDIKPRTLATFAPGCTYRGLAEDWLTDQGRRQMAFAIQEVRSYHAMFACTAAGSCVSV
CCCCCCCCHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
MPRGLVELMDHREAVEEKPLRTVDTYLASRPGFATSAFAAFRETLGEFSDFKEEGDGAG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDLAALAVFRTVAREQSVTRAAELLGRVPSNVTTRVQQLEAEIGVALFQRDKKRMVLTAA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GETYLDYAERILNLAEEAQQRVKPLAPFGTLRVGSMECAVASRLPAPLARYSAAWPQVRI
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHCCCHHHHHHCCCCEEEE
ELTTAPTRPLIEAVLARRIDCALIALPSGEGWLGPTPLDTVPVFREDLVLLLPHGHPEVH
EEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHCCEEEEECCCCCCCC
TAEDIKPRTLATFAPGCTYRGLAEDWLTDQGRRQMAFAIQEVRSYHAMFACTAAGSCVSV
CCCCCCCCHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
MPRGLVELMDHREAVEEKPLRTVDTYLASRPGFATSAFAAFRETLGEFSDFKEEGDGAG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]