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The map label for this gene is phaAB [H]

Identifier: 83592967

GI number: 83592967

Start: 1923094

End: 1924791

Strand: Reverse

Name: phaAB [H]

Synonym: Rru_A1632

Alternate gene names: 83592967

Gene position: 1924791-1923094 (Counterclockwise)

Preceding gene: 83592968

Following gene: 83592964

Centisome position: 44.22

GC content: 66.78

Gene sequence:

>1698_bases
ATGGCGATTGAGGCCCTGCCGCCGGCCCTGCTGATGATCCTGGGCGGGCTGATGCTGCCCCTGCTGCCCAAGCGCGCCCA
GGACCCCCTGATGGTCTTCCTGCCGCTGGCCACCCTGTGGGCGGTCTGGCAGATCGCCGATGGGCCGATGATGCTGGTGC
GCTTCCTGGATCTGGTGCTTGAACCCGTCCGCGGCGATGCGGTGGGCCGGCTGTTTGCCAGCGTGTTTTCACTGATGGCC
TTCGCCGGCGGCCTCTTCGCCCTCAAGCAGCCCCGCGTCCTCGAACGCGCCGCCGCCCTGGTCTATGCCGGAGGCGCCGT
CGGCTGCGCCCTGGCCGGCGATCTGATCAGCCTGTTCGTGTTTTGGGAGATCATGGCCGTCGCCTCGACCCTGGTCATCT
GGTCGTCGGACCAGCCGGCGGCGGCCAAGGCCGGGATGCGCTACGCCCTGATGCATCTGTTTGGCGGGGCGGTGCTGATG
GCCGGGATCGCCGGTCACGCGATCGGCGGCGGCAGTCTCGATGTCACGGCGATGACGCTCGACAGCCCGGCCAATTGGCT
GATCCTGATCGGCGTTCTGGTCAATGCCGGCGCCCCGCCGTTCTCGGCCTGGATCGCCGACGCCTATCCCGAAGGCTCGT
TCTCGGGCAGCGTTTTCCTGTCGGCCTTCACCACCAAAACGGCGGTGATGGTGCTGATCAAGGGCTTTCCGGGCACCGAG
CTGCTGATCTGGATCGGGCTGGCGATGGTGTTCTACGGCATCCTTTACGCCCTGCTTGAAAACGACATGCGGCGCATCCT
CAGCTATTCCATCGTCAATCAGGTGGGGTTCATGGTCTGCGCCGTCGGCATCGGCACCGATATGGCCCTGAACGGCGCCG
CCGCCCATGCCTTCGCCCATATCCTTTACAAGGCCCTGTTGCTGATGAGTGCCGGATCAGTGCTGATGATGACCGGCGGC
AAACGCAAATGCACCGAACTTGGCGGGCTCTACCGCTCGATGCCGATCACCACGGTCTGCGGCATCATCGGCGCCCTGGC
GATCTCGGCCTTTCCCTTCACCAGCGGCTTCGTCAGCAAATCGATGATCGCTCAAGGGGCGGCCGACCAGCATCTGGTCT
GGGTCTGGGCCCTGCTGCAGGCGGCGAGCGCCGGGGTGTTCCTGCATGCTGGCATCAAGTTTCCGTGGTTCGTCTTCTTC
CAGAAAGACAGCGGCCTGCGCCCGGCCGATCCGCCGGCCTCCATGCGCGCGGCGATGATCTTGCTGGCGGCGGCCTGCAT
CGGCCTTGGCGTTTTCCCCGGAGCCCTCTATGCCCTGCTGCCGGCGCCGACAGACTATCAGCCCTATACGGCCGACCATC
TGGTCAGCCAATTCCAATTGCTGCTGTTCGCCGGTCTGGCGTTTTTCGTGCTGCTGCCGTTGATGAAGCGCACCCGCACG
CTCAGCCTGGATATCGACTGGCTCTATCGCCGGCTGCTGCCCGATCTGCTGCGCGCCCTCGCCCCGCCGGCCAACCGCCT
TGGCGCGGCGCTCACCGCCCAGGCCCTGGCCGGGGTCAAGGCGGTGACGGGCGTGGTGGCGCGCAGCCATGGCCCCGACG
GGTCGTTCGGCCGACCCTGGCCGACCGGACGCTCGGTGGCGGTCATCCTCGGTCTGCTGGGCGCCTATCTGATCTTCGCC
GCCCTCGGCCACCCCTGA

Upstream 100 bases:

>100_bases
CTTTTCGCCTTCAACGCTTGGTTCGGCTTCCTCGCCTGCGTCGTGCTGGTGGCCATCGCCAAGCTTTTGGGCAGCGTTCT
TAAGCGCGGGGAAGAGTACT

Downstream 100 bases:

>100_bases
ACCCGATAAGGATCAGGGGCCGGGCGGCGGCGGCTTGGCCTCGAAAACCAGGCAATTCGACAGCGTGTCGTTGTGGAAGT
GGTAGGTCACGTTGTCTTCG

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol

Alternate protein names: pH adaptation potassium efflux system protein A/B; Pha system subunit A/B [H]

Number of amino acids: Translated: 565; Mature: 564

Protein sequence:

>565_residues
MAIEALPPALLMILGGLMLPLLPKRAQDPLMVFLPLATLWAVWQIADGPMMLVRFLDLVLEPVRGDAVGRLFASVFSLMA
FAGGLFALKQPRVLERAAALVYAGGAVGCALAGDLISLFVFWEIMAVASTLVIWSSDQPAAAKAGMRYALMHLFGGAVLM
AGIAGHAIGGGSLDVTAMTLDSPANWLILIGVLVNAGAPPFSAWIADAYPEGSFSGSVFLSAFTTKTAVMVLIKGFPGTE
LLIWIGLAMVFYGILYALLENDMRRILSYSIVNQVGFMVCAVGIGTDMALNGAAAHAFAHILYKALLLMSAGSVLMMTGG
KRKCTELGGLYRSMPITTVCGIIGALAISAFPFTSGFVSKSMIAQGAADQHLVWVWALLQAASAGVFLHAGIKFPWFVFF
QKDSGLRPADPPASMRAAMILLAAACIGLGVFPGALYALLPAPTDYQPYTADHLVSQFQLLLFAGLAFFVLLPLMKRTRT
LSLDIDWLYRRLLPDLLRALAPPANRLGAALTAQALAGVKAVTGVVARSHGPDGSFGRPWPTGRSVAVILGLLGAYLIFA
ALGHP

Sequences:

>Translated_565_residues
MAIEALPPALLMILGGLMLPLLPKRAQDPLMVFLPLATLWAVWQIADGPMMLVRFLDLVLEPVRGDAVGRLFASVFSLMA
FAGGLFALKQPRVLERAAALVYAGGAVGCALAGDLISLFVFWEIMAVASTLVIWSSDQPAAAKAGMRYALMHLFGGAVLM
AGIAGHAIGGGSLDVTAMTLDSPANWLILIGVLVNAGAPPFSAWIADAYPEGSFSGSVFLSAFTTKTAVMVLIKGFPGTE
LLIWIGLAMVFYGILYALLENDMRRILSYSIVNQVGFMVCAVGIGTDMALNGAAAHAFAHILYKALLLMSAGSVLMMTGG
KRKCTELGGLYRSMPITTVCGIIGALAISAFPFTSGFVSKSMIAQGAADQHLVWVWALLQAASAGVFLHAGIKFPWFVFF
QKDSGLRPADPPASMRAAMILLAAACIGLGVFPGALYALLPAPTDYQPYTADHLVSQFQLLLFAGLAFFVLLPLMKRTRT
LSLDIDWLYRRLLPDLLRALAPPANRLGAALTAQALAGVKAVTGVVARSHGPDGSFGRPWPTGRSVAVILGLLGAYLIFA
ALGHP
>Mature_564_residues
AIEALPPALLMILGGLMLPLLPKRAQDPLMVFLPLATLWAVWQIADGPMMLVRFLDLVLEPVRGDAVGRLFASVFSLMAF
AGGLFALKQPRVLERAAALVYAGGAVGCALAGDLISLFVFWEIMAVASTLVIWSSDQPAAAKAGMRYALMHLFGGAVLMA
GIAGHAIGGGSLDVTAMTLDSPANWLILIGVLVNAGAPPFSAWIADAYPEGSFSGSVFLSAFTTKTAVMVLIKGFPGTEL
LIWIGLAMVFYGILYALLENDMRRILSYSIVNQVGFMVCAVGIGTDMALNGAAAHAFAHILYKALLLMSAGSVLMMTGGK
RKCTELGGLYRSMPITTVCGIIGALAISAFPFTSGFVSKSMIAQGAADQHLVWVWALLQAASAGVFLHAGIKFPWFVFFQ
KDSGLRPADPPASMRAAMILLAAACIGLGVFPGALYALLPAPTDYQPYTADHLVSQFQLLLFAGLAFFVLLPLMKRTRTL
SLDIDWLYRRLLPDLLRALAPPANRLGAALTAQALAGVKAVTGVVARSHGPDGSFGRPWPTGRSVAVILGLLGAYLIFAA
LGHP

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the CPA3 antiporters (TC 2.A.63) subunit B family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=281, Percent_Identity=27.0462633451957, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1788827, Length=349, Percent_Identity=26.9340974212034, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788614, Length=319, Percent_Identity=28.5266457680251, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788613, Length=292, Percent_Identity=27.0547945205479, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788829, Length=348, Percent_Identity=26.4367816091954, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788831, Length=211, Percent_Identity=33.6492890995261, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI2367154, Length=139, Percent_Identity=31.6546762589928, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR005281
- InterPro:   IPR007182
- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF04039 MnhB; PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 59653; Mature: 59522

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEALPPALLMILGGLMLPLLPKRAQDPLMVFLPLATLWAVWQIADGPMMLVRFLDLVL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EPVRGDAVGRLFASVFSLMAFAGGLFALKQPRVLERAAALVYAGGAVGCALAGDLISLFV
HHCCCHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FWEIMAVASTLVIWSSDQPAAAKAGMRYALMHLFGGAVLMAGIAGHAIGGGSLDVTAMTL
HHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
DSPANWLILIGVLVNAGAPPFSAWIADAYPEGSFSGSVFLSAFTTKTAVMVLIKGFPGTE
CCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHEEECCCCCH
LLIWIGLAMVFYGILYALLENDMRRILSYSIVNQVGFMVCAVGIGTDMALNGAAAHAFAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHH
ILYKALLLMSAGSVLMMTGGKRKCTELGGLYRSMPITTVCGIIGALAISAFPFTSGFVSK
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHH
SMIAQGAADQHLVWVWALLQAASAGVFLHAGIKFPWFVFFQKDSGLRPADPPASMRAAMI
HHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHH
LLAAACIGLGVFPGALYALLPAPTDYQPYTADHLVSQFQLLLFAGLAFFVLLPLMKRTRT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
LSLDIDWLYRRLLPDLLRALAPPANRLGAALTAQALAGVKAVTGVVARSHGPDGSFGRPW
EEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PTGRSVAVILGLLGAYLIFAALGHP
CCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIEALPPALLMILGGLMLPLLPKRAQDPLMVFLPLATLWAVWQIADGPMMLVRFLDLVL
CCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EPVRGDAVGRLFASVFSLMAFAGGLFALKQPRVLERAAALVYAGGAVGCALAGDLISLFV
HHCCCHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FWEIMAVASTLVIWSSDQPAAAKAGMRYALMHLFGGAVLMAGIAGHAIGGGSLDVTAMTL
HHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
DSPANWLILIGVLVNAGAPPFSAWIADAYPEGSFSGSVFLSAFTTKTAVMVLIKGFPGTE
CCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHEEECCCCCH
LLIWIGLAMVFYGILYALLENDMRRILSYSIVNQVGFMVCAVGIGTDMALNGAAAHAFAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHH
ILYKALLLMSAGSVLMMTGGKRKCTELGGLYRSMPITTVCGIIGALAISAFPFTSGFVSK
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHH
SMIAQGAADQHLVWVWALLQAASAGVFLHAGIKFPWFVFFQKDSGLRPADPPASMRAAMI
HHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHH
LLAAACIGLGVFPGALYALLPAPTDYQPYTADHLVSQFQLLLFAGLAFFVLLPLMKRTRT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
LSLDIDWLYRRLLPDLLRALAPPANRLGAALTAQALAGVKAVTGVVARSHGPDGSFGRPW
EEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PTGRSVAVILGLLGAYLIFAALGHP
CCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]