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The map label for this gene is glgX [H]

Identifier: 83592941

GI number: 83592941

Start: 1896674

End: 1898863

Strand: Direct

Name: glgX [H]

Synonym: Rru_A1606

Alternate gene names: 83592941

Gene position: 1896674-1898863 (Clockwise)

Preceding gene: 83592940

Following gene: 83592942

Centisome position: 43.57

GC content: 63.84

Gene sequence:

>2190_bases
ATGCCGCTTACGCGACCGCGAGTCTGGCCCGGCCGCCCCTATCCCCTTGGCGGGACCTGGGATGGCCGGGGGGTCAATTT
TGCGTTGTTTTCCGAGCATGCGGAAAAGGTGGAACTTTGCCTTTTCGACGGGGATGACGGCCGGGAACTCTCGCGCGTTC
CTCTTCCCGAATACACCGATGAGATTTGGCATGGCTATCTGCCCGACGTTCGCCCGGGCCAGCGCTACGGCTATCGGGTT
TACGGCCCCTATGCCCCCCATGCCGGCCATCGTTTCAATCCCAACAAGCTGGTGCTCGACCCTTACGCCCGTTCGTGGGG
CGGGCGCCTGCAGTGGACCGATGCCCATTTCGGCTATCGCTTCGGCGCGGCGTCGGAAGATCTGACCTTCGACACCCGCG
ACAATTCCGCCTTCATGCCCAAATGCGTCATCGTCGAAACCGCCTTCACCTGGGATGACGACCGCGCCCCGCGCCGGCCC
TGGCACGACAGCGTGTTGTACGAGCTTCACGTCAAGGGCTTCACCATCCGCCATCCCGAGGTGCCGCGCGCCATTCGCGG
CACCTTCGCCGGCATGTCGACCCAGGCGGTGGTGTCCTATCTGAAATCCCTGGGCATCACGGCGATCGAGCTTTTGCCGG
TTCAGGCCTTTGTCGATGAGCGCCATCTGGTCAAGAACAACTTGACCAATTATTGGGGTTACGCCTCGCTGGGTTATTTC
GCCCCCCAGCCGTCCTATCTGGCCAGCGGCAAGCTGGGCGAGTTCAAGACCTTCGTGCAGGTCATGCATGACGCCGGCAT
CGAGGTTATCCTTGATGTCGTTTACAACCATACGGCCGAGGGCAATCACCTTGGCCCGACCTTCAGTTTCAAAGGCATCG
ACAACGCCAGCTATTACCGGCTGGTGCCGGGCAACGAGCGTTTCTACGCCGACAGCACGGGCTGCGGCAACGCGCTCAAC
CTGCGCCACCCCCGGGTTTTGCAGATGGTCATGGACTCGCTGCGCTATTGGGTGCGCGAGATGCATGTCGATGGTTTCCG
CTTCGATCTGGCGACGACCCTGGCCCGCGACCACGGCCGCTTCGATCCCCATTCCGCCTTTATCGAGGCCCTGCGCCAGG
ATCCGGTGCTCTCCACCGTCAAGCTGATCGCCGAGCCCTGGGACGTCGGCGATGGCGGCTACCGGCTGGGCGGCTTCCCC
CCGGGCTTCGCCGAATGGAATGACCGTTACCGCGATACCGTGCGGCGCTTTTGGAAGGGCGATCGCGGGCAGGTGGCCGA
TCTGGCGACCCGGCTGACCGGGTCTTCGGACCTGTTCGCCAATCGCGGCCGCTGCCCCTGGGCCAGCATCAATTTCGTCA
CCGCCCATGACGGGTTCACCCTGGCCGATCTTGTCTCCTACAACGGCAAGCACAACGAGGCCAATGGCGAGAACAACCGG
GACGGCACGGATAACAACAATTCGTGGAACCACGGGATTGAAGGGCCGACCTCGGATCCGTCGATCCAGGCCTTGCGCCG
TCGGCAGGTGCGGAACTTCCTTGCCACCTTGCTGTTGTCGCAAGGGGTGCCGATGCTGGTGGCCGGCGATGAATTCGGGC
GCAGCCAGCGCGGCAACAACAATCCCTACTGCCAGGACAACGAGATTTCCTGGATCAATTGGGCGGCGATCGATGCCGAG
GGCCAGTCCCTCGCCCGCATGGTGCGCTGGCTGATACGCTTGCGCCGGCGCCATATCGTCTTTCATCGCAACCGCTTCTT
TCACGGAACGACCCTGCGCGGCACCGATGTCAAGGACATCACCTGGCTTGAGCCCGACGGCCGCGAGCGCTCCGATGCCC
GCGATTGGACCGACCCGGAAGAGCGTTTCCTGGCCTTTCTCATCCGCGGCGAGGCCGGCGAGTACTTTGTGACCGAAATG
GGCGATCCCGAGCCCGATCATAGCTTTTTGGTAGCCCTGAACGCCGATTCCCGCCCGGTGCCGATGCTTCTGCCCGTGCT
GACGGCGGGCACGCGCTGGGTCTTGCTGTTCGACACGGCGCGGCCGGGGGCGGAACGGCGCCCGGCCCCGGCCGACGGCA
AGATGTATTCGGTGGAGGGGCGATCGCTGACGGTGTTCCGGCGCGAGCCGGGACTGCGGGATGGGGATGGGGATGGTCCC
TGGGAATTGGATGGCTTCCCCGAGGTATGA

Upstream 100 bases:

>100_bases
CGCAGATATTTTGATCTGAATTCAATAAGATAGGGTGGCTAAAGCGCGGATTGGCCGGTGACCGGTCGTCCGCTTGCCGC
CCTAAGGAGGACGTCCCAGC

Downstream 100 bases:

>100_bases
AGGGGGGGCCCAAGGGTGGAGAGAGGGACGGATGACGGACGGCACCCCCGGAGTATTGAGGGTTCTTGCGGAACGGGCAG
GGGTTATCGACCGCTGTTAT

Product: glycogen debranching protein GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 729; Mature: 728

Protein sequence:

>729_residues
MPLTRPRVWPGRPYPLGGTWDGRGVNFALFSEHAEKVELCLFDGDDGRELSRVPLPEYTDEIWHGYLPDVRPGQRYGYRV
YGPYAPHAGHRFNPNKLVLDPYARSWGGRLQWTDAHFGYRFGAASEDLTFDTRDNSAFMPKCVIVETAFTWDDDRAPRRP
WHDSVLYELHVKGFTIRHPEVPRAIRGTFAGMSTQAVVSYLKSLGITAIELLPVQAFVDERHLVKNNLTNYWGYASLGYF
APQPSYLASGKLGEFKTFVQVMHDAGIEVILDVVYNHTAEGNHLGPTFSFKGIDNASYYRLVPGNERFYADSTGCGNALN
LRHPRVLQMVMDSLRYWVREMHVDGFRFDLATTLARDHGRFDPHSAFIEALRQDPVLSTVKLIAEPWDVGDGGYRLGGFP
PGFAEWNDRYRDTVRRFWKGDRGQVADLATRLTGSSDLFANRGRCPWASINFVTAHDGFTLADLVSYNGKHNEANGENNR
DGTDNNNSWNHGIEGPTSDPSIQALRRRQVRNFLATLLLSQGVPMLVAGDEFGRSQRGNNNPYCQDNEISWINWAAIDAE
GQSLARMVRWLIRLRRRHIVFHRNRFFHGTTLRGTDVKDITWLEPDGRERSDARDWTDPEERFLAFLIRGEAGEYFVTEM
GDPEPDHSFLVALNADSRPVPMLLPVLTAGTRWVLLFDTARPGAERRPAPADGKMYSVEGRSLTVFRREPGLRDGDGDGP
WELDGFPEV

Sequences:

>Translated_729_residues
MPLTRPRVWPGRPYPLGGTWDGRGVNFALFSEHAEKVELCLFDGDDGRELSRVPLPEYTDEIWHGYLPDVRPGQRYGYRV
YGPYAPHAGHRFNPNKLVLDPYARSWGGRLQWTDAHFGYRFGAASEDLTFDTRDNSAFMPKCVIVETAFTWDDDRAPRRP
WHDSVLYELHVKGFTIRHPEVPRAIRGTFAGMSTQAVVSYLKSLGITAIELLPVQAFVDERHLVKNNLTNYWGYASLGYF
APQPSYLASGKLGEFKTFVQVMHDAGIEVILDVVYNHTAEGNHLGPTFSFKGIDNASYYRLVPGNERFYADSTGCGNALN
LRHPRVLQMVMDSLRYWVREMHVDGFRFDLATTLARDHGRFDPHSAFIEALRQDPVLSTVKLIAEPWDVGDGGYRLGGFP
PGFAEWNDRYRDTVRRFWKGDRGQVADLATRLTGSSDLFANRGRCPWASINFVTAHDGFTLADLVSYNGKHNEANGENNR
DGTDNNNSWNHGIEGPTSDPSIQALRRRQVRNFLATLLLSQGVPMLVAGDEFGRSQRGNNNPYCQDNEISWINWAAIDAE
GQSLARMVRWLIRLRRRHIVFHRNRFFHGTTLRGTDVKDITWLEPDGRERSDARDWTDPEERFLAFLIRGEAGEYFVTEM
GDPEPDHSFLVALNADSRPVPMLLPVLTAGTRWVLLFDTARPGAERRPAPADGKMYSVEGRSLTVFRREPGLRDGDGDGP
WELDGFPEV
>Mature_728_residues
PLTRPRVWPGRPYPLGGTWDGRGVNFALFSEHAEKVELCLFDGDDGRELSRVPLPEYTDEIWHGYLPDVRPGQRYGYRVY
GPYAPHAGHRFNPNKLVLDPYARSWGGRLQWTDAHFGYRFGAASEDLTFDTRDNSAFMPKCVIVETAFTWDDDRAPRRPW
HDSVLYELHVKGFTIRHPEVPRAIRGTFAGMSTQAVVSYLKSLGITAIELLPVQAFVDERHLVKNNLTNYWGYASLGYFA
PQPSYLASGKLGEFKTFVQVMHDAGIEVILDVVYNHTAEGNHLGPTFSFKGIDNASYYRLVPGNERFYADSTGCGNALNL
RHPRVLQMVMDSLRYWVREMHVDGFRFDLATTLARDHGRFDPHSAFIEALRQDPVLSTVKLIAEPWDVGDGGYRLGGFPP
GFAEWNDRYRDTVRRFWKGDRGQVADLATRLTGSSDLFANRGRCPWASINFVTAHDGFTLADLVSYNGKHNEANGENNRD
GTDNNNSWNHGIEGPTSDPSIQALRRRQVRNFLATLLLSQGVPMLVAGDEFGRSQRGNNNPYCQDNEISWINWAAIDAEG
QSLARMVRWLIRLRRRHIVFHRNRFFHGTTLRGTDVKDITWLEPDGRERSDARDWTDPEERFLAFLIRGEAGEYFVTEMG
DPEPDHSFLVALNADSRPVPMLLPVLTAGTRWVLLFDTARPGAERRPAPADGKMYSVEGRSLTVFRREPGLRDGDGDGPW
ELDGFPEV

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=580, Percent_Identity=51.0344827586207, Blast_Score=569, Evalue=1e-163,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 82717; Mature: 82586

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTRPRVWPGRPYPLGGTWDGRGVNFALFSEHAEKVELCLFDGDDGRELSRVPLPEYTD
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCHHHH
EIWHGYLPDVRPGQRYGYRVYGPYAPHAGHRFNPNKLVLDPYARSWGGRLQWTDAHFGYR
HHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEECCHHHCCCCEEEEECCCCCEE
FGAASEDLTFDTRDNSAFMPKCVIVETAFTWDDDRAPRRPWHDSVLYELHVKGFTIRHPE
ECCCCCCCEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEECCC
VPRAIRGTFAGMSTQAVVSYLKSLGITAIELLPVQAFVDERHLVKNNLTNYWGYASLGYF
CCHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCC
APQPSYLASGKLGEFKTFVQVMHDAGIEVILDVVYNHTAEGNHLGPTFSFKGIDNASYYR
CCCCCHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCEEE
LVPGNERFYADSTGCGNALNLRHPRVLQMVMDSLRYWVREMHVDGFRFDLATTLARDHGR
EECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCC
FDPHSAFIEALRQDPVLSTVKLIAEPWDVGDGGYRLGGFPPGFAEWNDRYRDTVRRFWKG
CCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCC
DRGQVADLATRLTGSSDLFANRGRCPWASINFVTAHDGFTLADLVSYNGKHNEANGENNR
CCCHHHHHHHHHCCCCHHHCCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCCCCCCC
DGTDNNNSWNHGIEGPTSDPSIQALRRRQVRNFLATLLLSQGVPMLVAGDEFGRSQRGNN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCC
NPYCQDNEISWINWAAIDAEGQSLARMVRWLIRLRRRHIVFHRNRFFHGTTLRGTDVKDI
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHEEEECCCEEECCEECCCCCCCE
TWLEPDGRERSDARDWTDPEERFLAFLIRGEAGEYFVTEMGDPEPDHSFLVALNADSRPV
EEECCCCCCCCCCCCCCCHHHHEEEEEEECCCCCEEEECCCCCCCCCCEEEEECCCCCCC
PMLLPVLTAGTRWVLLFDTARPGAERRPAPADGKMYSVEGRSLTVFRREPGLRDGDGDGP
CHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCCCCCCC
WELDGFPEV
CCCCCCCCC
>Mature Secondary Structure 
PLTRPRVWPGRPYPLGGTWDGRGVNFALFSEHAEKVELCLFDGDDGRELSRVPLPEYTD
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCHHHH
EIWHGYLPDVRPGQRYGYRVYGPYAPHAGHRFNPNKLVLDPYARSWGGRLQWTDAHFGYR
HHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEECCHHHCCCCEEEEECCCCCEE
FGAASEDLTFDTRDNSAFMPKCVIVETAFTWDDDRAPRRPWHDSVLYELHVKGFTIRHPE
ECCCCCCCEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEECCC
VPRAIRGTFAGMSTQAVVSYLKSLGITAIELLPVQAFVDERHLVKNNLTNYWGYASLGYF
CCHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCC
APQPSYLASGKLGEFKTFVQVMHDAGIEVILDVVYNHTAEGNHLGPTFSFKGIDNASYYR
CCCCCHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCEEE
LVPGNERFYADSTGCGNALNLRHPRVLQMVMDSLRYWVREMHVDGFRFDLATTLARDHGR
EECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCC
FDPHSAFIEALRQDPVLSTVKLIAEPWDVGDGGYRLGGFPPGFAEWNDRYRDTVRRFWKG
CCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCC
DRGQVADLATRLTGSSDLFANRGRCPWASINFVTAHDGFTLADLVSYNGKHNEANGENNR
CCCHHHHHHHHHCCCCHHHCCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCCCCCCC
DGTDNNNSWNHGIEGPTSDPSIQALRRRQVRNFLATLLLSQGVPMLVAGDEFGRSQRGNN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCC
NPYCQDNEISWINWAAIDAEGQSLARMVRWLIRLRRRHIVFHRNRFFHGTTLRGTDVKDI
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHEEEECCCEEECCEECCCCCCCE
TWLEPDGRERSDARDWTDPEERFLAFLIRGEAGEYFVTEMGDPEPDHSFLVALNADSRPV
EEECCCCCCCCCCCCCCCHHHHEEEEEEECCCCCEEEECCCCCCCCCCEEEEECCCCCCC
PMLLPVLTAGTRWVLLFDTARPGAERRPAPADGKMYSVEGRSLTVFRREPGLRDGDGDGP
CHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCCCCCCC
WELDGFPEV
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]