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The map label for this gene is pta [H]

Identifier: 83592804

GI number: 83592804

Start: 1734502

End: 1735938

Strand: Reverse

Name: pta [H]

Synonym: Rru_A1468

Alternate gene names: 83592804

Gene position: 1735938-1734502 (Counterclockwise)

Preceding gene: 83592805

Following gene: 83592803

Centisome position: 39.88

GC content: 67.36

Gene sequence:

>1437_bases
ATGACGACGGTTTCGCCTGATGACTCCTCACCTTCAACGCTGACCAGCCTGACCTTCGATGATCTGACCCCCGGTCAGTC
GGCCAGTCTGTCGCGAACCTTGACCCAGGCCGATCTCGATCTGTTCGCCACCGTCTCGGGCAATATCGATCCCTCGCATT
TCGCCGATCCACCGGCGGGAACGACGCTGTTTCCCAAGGCCATCGGTCACGGCATGTGGTCGGCGTCGTTGATCTCGGGG
GTTCTGGGCACCCGGTTGCCCGGACCGGGGACCGTCTATCTGGCCCAGGACCTGCGCTTCCGCGCGCCCGTCACCTTGGG
CGATACGATCACCGCCAGCGTAACCGTTCGCGAGAAGCGCACCGAGCGCAAGACCGTGGTTTTCGAGTGCCTGTGCACCA
ATCAGAACGGCGTCACCGTCGTCTCGGGCACGGCCGAGGTGATCGCCCCGGCCACCCACGCCTCGGTCGCCGCCCAGGCC
CTGCCCGGGCTGCAGATGATCCGTCACGACCGACAAGCCGCCCTGCTGGCGCGCTGCGCCGGCTTGCCCGCCCTGCCGAC
GGCGGTGATCCATCCCTGTGACGGCCCGGCCCTGCAAGGGGCGGTGGAAGCGGCGCAGCGGGGATTGATCGAGCCGATCC
TCGTCGGCCCTTCGTTGCTGATCGCCGCCACCGCCAAGGCGCTGGGCCTGGACATCTCGGCCTTCCGTCTTGTCGAAGCC
GCCAACGGAGCGGCGGCGGCGGCGACCGGCGTCGCCCTGGTCCGCGCCGGCGAGGCGCGAGCGGTGATGAAGGGCGCGCT
GCATACCGACGAGTTGATGGCCGCCGTGGTCAAGCGCGATGGCGGCCTGCGCACGGCGGGGCGGATCAGCCATGTCTTTC
TGATGACCGTGCCGACGTACCCCAAACCGCTGCTGATCACCGATGCGGTGGTCAACATCGCGCCCTCGCTGGAAGACAAG
GTCCACATCGTTCAGAACGCCATCGATCTCGCCCGCATCCTGGGTGTCGAAACGCCCAAGGTGGCGATCCTCTCGGCGGT
CGAGACCATCAATCCCAAGATCTCCTCGACCGTCGATGCCGCGGCCCTGTGCAAGATGGCCCAGCGCGGCCAGATCCGGG
GGGGATTGCTCGATGGCCCTCTGGCTTTCGACAACGCCATTTCCGCCGAGGCGGCGAAGACCAAGGGCATCGTCTCCGAG
GTCGCCGGTCAGGCCGATATCCTGCTCGTGCCCGATCTCGAGTCCGGCAACATGCTGGCCAAGCAGTTGTCCTTCCTCGC
CCATGCCGATGCCGCCGGCATCGTGCTTGGCACCAGCGCGCCGGTGATGCTGACCAGCCGCGCCGACAATCTGGCGGCCC
GTCTCGCCTCCTGCGCCCTCGCCGTTCTGATCGATGCCGCGTCACGGCCTCGTTCCACCGTTCTCGCCGCCGAGTAA

Upstream 100 bases:

>100_bases
CCGTCTCTTTCGTTTTCCCTCCCACCCGCTCCGCCGTCCTGGCGACGACGGAGCGCAAGGCTATTTTTTTGCCCTTTTCC
TCAACCAAAAGGTGATGTTT

Downstream 100 bases:

>100_bases
GGAGACCCGCCCATGTTTCCCCCCTCCCCCAAGCAGGCGTTCCTCGTTCTCAACAGCGGCTCGTCGAGCATCAAGTTCTC
GGTTTTTGAAAAGACCAGCG

Product: bifunctional enoyl-CoA hydratase/phosphate acetyltransferase

Products: NA

Alternate protein names: Phosphotransacetylase [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MTTVSPDDSSPSTLTSLTFDDLTPGQSASLSRTLTQADLDLFATVSGNIDPSHFADPPAGTTLFPKAIGHGMWSASLISG
VLGTRLPGPGTVYLAQDLRFRAPVTLGDTITASVTVREKRTERKTVVFECLCTNQNGVTVVSGTAEVIAPATHASVAAQA
LPGLQMIRHDRQAALLARCAGLPALPTAVIHPCDGPALQGAVEAAQRGLIEPILVGPSLLIAATAKALGLDISAFRLVEA
ANGAAAAATGVALVRAGEARAVMKGALHTDELMAAVVKRDGGLRTAGRISHVFLMTVPTYPKPLLITDAVVNIAPSLEDK
VHIVQNAIDLARILGVETPKVAILSAVETINPKISSTVDAAALCKMAQRGQIRGGLLDGPLAFDNAISAEAAKTKGIVSE
VAGQADILLVPDLESGNMLAKQLSFLAHADAAGIVLGTSAPVMLTSRADNLAARLASCALAVLIDAASRPRSTVLAAE

Sequences:

>Translated_478_residues
MTTVSPDDSSPSTLTSLTFDDLTPGQSASLSRTLTQADLDLFATVSGNIDPSHFADPPAGTTLFPKAIGHGMWSASLISG
VLGTRLPGPGTVYLAQDLRFRAPVTLGDTITASVTVREKRTERKTVVFECLCTNQNGVTVVSGTAEVIAPATHASVAAQA
LPGLQMIRHDRQAALLARCAGLPALPTAVIHPCDGPALQGAVEAAQRGLIEPILVGPSLLIAATAKALGLDISAFRLVEA
ANGAAAAATGVALVRAGEARAVMKGALHTDELMAAVVKRDGGLRTAGRISHVFLMTVPTYPKPLLITDAVVNIAPSLEDK
VHIVQNAIDLARILGVETPKVAILSAVETINPKISSTVDAAALCKMAQRGQIRGGLLDGPLAFDNAISAEAAKTKGIVSE
VAGQADILLVPDLESGNMLAKQLSFLAHADAAGIVLGTSAPVMLTSRADNLAARLASCALAVLIDAASRPRSTVLAAE
>Mature_477_residues
TTVSPDDSSPSTLTSLTFDDLTPGQSASLSRTLTQADLDLFATVSGNIDPSHFADPPAGTTLFPKAIGHGMWSASLISGV
LGTRLPGPGTVYLAQDLRFRAPVTLGDTITASVTVREKRTERKTVVFECLCTNQNGVTVVSGTAEVIAPATHASVAAQAL
PGLQMIRHDRQAALLARCAGLPALPTAVIHPCDGPALQGAVEAAQRGLIEPILVGPSLLIAATAKALGLDISAFRLVEAA
NGAAAAATGVALVRAGEARAVMKGALHTDELMAAVVKRDGGLRTAGRISHVFLMTVPTYPKPLLITDAVVNIAPSLEDKV
HIVQNAIDLARILGVETPKVAILSAVETINPKISSTVDAAALCKMAQRGQIRGGLLDGPLAFDNAISAEAAKTKGIVSEV
AGQADILLVPDLESGNMLAKQLSFLAHADAAGIVLGTSAPVMLTSRADNLAARLASCALAVLIDAASRPRSTVLAAE

Specific function: Conversion of acetate to acetyl-CoA; second step. [C]

COG id: COG0280

COG function: function code C; Phosphotransacetylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788635, Length=309, Percent_Identity=27.5080906148867, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: 1420 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1250 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012147
- InterPro:   IPR002505 [H]

Pfam domain/function: PF01515 PTA_PTB [H]

EC number: =2.3.1.8 [H]

Molecular weight: Translated: 49018; Mature: 48887

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVSPDDSSPSTLTSLTFDDLTPGQSASLSRTLTQADLDLFATVSGNIDPSHFADPPAG
CCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCEEEEEECCCCCHHHCCCCCCC
TTLFPKAIGHGMWSASLISGVLGTRLPGPGTVYLAQDLRFRAPVTLGDTITASVTVREKR
CEECHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCEEECCEEECCCEEEEEEEHHHH
TERKTVVFECLCTNQNGVTVVSGTAEVIAPATHASVAAQALPGLQMIRHDRQAALLARCA
CCCCEEEEEEEECCCCCEEEEECCHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
GLPALPTAVIHPCDGPALQGAVEAAQRGLIEPILVGPSLLIAATAKALGLDISAFRLVEA
CCCCCCHHEEECCCCHHHHHHHHHHHCCCCCEEHHCCHHHHHHHHHHHCCCHHHHHHHHH
ANGAAAAATGVALVRAGEARAVMKGALHTDELMAAVVKRDGGLRTAGRISHVFLMTVPTY
CCCCHHHHHCCCEEECCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCC
PKPLLITDAVVNIAPSLEDKVHIVQNAIDLARILGVETPKVAILSAVETINPKISSTVDA
CCCEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHH
AALCKMAQRGQIRGGLLDGPLAFDNAISAEAAKTKGIVSEVAGQADILLVPDLESGNMLA
HHHHHHHHCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHH
KQLSFLAHADAAGIVLGTSAPVMLTSRADNLAARLASCALAVLIDAASRPRSTVLAAE
HHHHHHHCCCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure 
TTVSPDDSSPSTLTSLTFDDLTPGQSASLSRTLTQADLDLFATVSGNIDPSHFADPPAG
CCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCEEEEEECCCCCHHHCCCCCCC
TTLFPKAIGHGMWSASLISGVLGTRLPGPGTVYLAQDLRFRAPVTLGDTITASVTVREKR
CEECHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCEEECCEEECCCEEEEEEEHHHH
TERKTVVFECLCTNQNGVTVVSGTAEVIAPATHASVAAQALPGLQMIRHDRQAALLARCA
CCCCEEEEEEEECCCCCEEEEECCHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
GLPALPTAVIHPCDGPALQGAVEAAQRGLIEPILVGPSLLIAATAKALGLDISAFRLVEA
CCCCCCHHEEECCCCHHHHHHHHHHHCCCCCEEHHCCHHHHHHHHHHHCCCHHHHHHHHH
ANGAAAAATGVALVRAGEARAVMKGALHTDELMAAVVKRDGGLRTAGRISHVFLMTVPTY
CCCCHHHHHCCCEEECCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCC
PKPLLITDAVVNIAPSLEDKVHIVQNAIDLARILGVETPKVAILSAVETINPKISSTVDA
CCCEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHH
AALCKMAQRGQIRGGLLDGPLAFDNAISAEAAKTKGIVSEVAGQADILLVPDLESGNMLA
HHHHHHHHCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHH
KQLSFLAHADAAGIVLGTSAPVMLTSRADNLAARLASCALAVLIDAASRPRSTVLAAE
HHHHHHHCCCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]