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The map label for this gene is cheB2

Identifier: 83592742

GI number: 83592742

Start: 1660519

End: 1661610

Strand: Direct

Name: cheB2

Synonym: Rru_A1406

Alternate gene names: 83592742

Gene position: 1660519-1661610 (Clockwise)

Preceding gene: 83592741

Following gene: 83592743

Centisome position: 38.15

GC content: 65.57

Gene sequence:

>1092_bases
ATGCCCCGCAAAATCCGTGTCCTGATTGTCGATGATTCCGCCTCCGTTCGCCAGACGATGACCGAGATCCTGGAATCCGA
TCCGGGGATCGAGGTGATCGGCACCGCGCCCGACCCTTATGTCGCCGCCCGGCGCATCCAGCAGGAAGTGCCCGATGTCA
TCACCCTTGATGTCGAGATGCCGCGCATGGACGGCATCACCTTTCTGCGCAAGATCATGGCCCAGCGGCCGATTCCGGTG
GTCGTGTGCTCCTCGTTGACCGAAACGGATTCGGAAACCGCCTCGCAGGCCTGGGAGGCCGGGGCGGTCGAGATCATTCT
CAAGCCCCGCGTCGGCACCGCCCAGTTCCTTCTGGAATCGAAAATCCATATCTGCGACGTGGTCAAGGCCGCCGCCGGCG
CCCGTCTGCGCTCCATGCCCGCTGCGGGGCGCGCCAACCGGCCAAGGGTGCCCGAAAAGAAGCTGACCGCCGACGCCGTT
CTGCCGCCGCCGGTGGCCGGGCGCAACGCCATGGCCAGAACCACGGAATCGGTCATCTGCATCGGCGCCTCGACCGGCGG
CACCGAATCCCTGCGCGAGGTGCTGGAAGCCCTGCCGGCGGCCAGCCCGGCCATCGTCATCGTCCAGCACATGCCCGAGA
AATTCACCGAGGCGTTCGCCCGCCGCCTCGATAGCCTGTGTGACATGGAGGTCAAGGAGGCCGTCGATGGCGATACGGTG
ATGCGCGGCCGGGTGCTGATCGCGCCGGGCAACCACCACATGTTGCTCCAGCGCAGCGGAGCGCGGTACTATGTCTCGGT
CAAGGATGGTCCCTTGGTCTCGCGTCACCGTCCTTCGGTCGATGTGTTGTTCCGCTCGGCGGCCAGTCACGCCGGTTCGA
ACGCCGTGGGCATCATCATGACCGGCATGGGTGACGATGGGGCGCGCGGATTGCTCGAGATGCGAAAGGCCGGCGCCTAT
ACCATCGCCCAGGACGAAGCCACCTCGGTGGTTTTCGGCATGCCGAAGGAAGCGATCGCCCTGGGGGCGGCCGACAAGAT
CCTGCCGCTCGAGATGCTGGCGATGGAAATTCTGCGCGCCGGTAACCGATGA

Upstream 100 bases:

>100_bases
CCCCGGAGGATATCTGTTCCTTGGACATTCGGAATCCCTGGCGGGGTTTGGCTTGCCGCTGAAAGCGGTCGGTACCACCG
TTTTTCAACGCGAGTAGCCC

Downstream 100 bases:

>100_bases
CCTGTCTTTCCCCGTTGTGGCAGGGCTTTTCGCCGATGGTCAATTCGATGCCCAAGCCCTTTCTGACCCCTGGAACGCTC
TATTGCGGCGCCGCGCCGGC

Product: two component CheB methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MPRKIRVLIVDDSASVRQTMTEILESDPGIEVIGTAPDPYVAARRIQQEVPDVITLDVEMPRMDGITFLRKIMAQRPIPV
VVCSSLTETDSETASQAWEAGAVEIILKPRVGTAQFLLESKIHICDVVKAAAGARLRSMPAAGRANRPRVPEKKLTADAV
LPPPVAGRNAMARTTESVICIGASTGGTESLREVLEALPAASPAIVIVQHMPEKFTEAFARRLDSLCDMEVKEAVDGDTV
MRGRVLIAPGNHHMLLQRSGARYYVSVKDGPLVSRHRPSVDVLFRSAASHAGSNAVGIIMTGMGDDGARGLLEMRKAGAY
TIAQDEATSVVFGMPKEAIALGAADKILPLEMLAMEILRAGNR

Sequences:

>Translated_363_residues
MPRKIRVLIVDDSASVRQTMTEILESDPGIEVIGTAPDPYVAARRIQQEVPDVITLDVEMPRMDGITFLRKIMAQRPIPV
VVCSSLTETDSETASQAWEAGAVEIILKPRVGTAQFLLESKIHICDVVKAAAGARLRSMPAAGRANRPRVPEKKLTADAV
LPPPVAGRNAMARTTESVICIGASTGGTESLREVLEALPAASPAIVIVQHMPEKFTEAFARRLDSLCDMEVKEAVDGDTV
MRGRVLIAPGNHHMLLQRSGARYYVSVKDGPLVSRHRPSVDVLFRSAASHAGSNAVGIIMTGMGDDGARGLLEMRKAGAY
TIAQDEATSVVFGMPKEAIALGAADKILPLEMLAMEILRAGNR
>Mature_362_residues
PRKIRVLIVDDSASVRQTMTEILESDPGIEVIGTAPDPYVAARRIQQEVPDVITLDVEMPRMDGITFLRKIMAQRPIPVV
VCSSLTETDSETASQAWEAGAVEIILKPRVGTAQFLLESKIHICDVVKAAAGARLRSMPAAGRANRPRVPEKKLTADAVL
PPPVAGRNAMARTTESVICIGASTGGTESLREVLEALPAASPAIVIVQHMPEKFTEAFARRLDSLCDMEVKEAVDGDTVM
RGRVLIAPGNHHMLLQRSGARYYVSVKDGPLVSRHRPSVDVLFRSAASHAGSNAVGIIMTGMGDDGARGLLEMRKAGAYT
IAQDEATSVVFGMPKEAIALGAADKILPLEMLAMEILRAGNR

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=355, Percent_Identity=50.7042253521127, Blast_Score=344, Evalue=5e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB2_RHORT (Q2RUI8)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426494.1
- ProteinModelPortal:   Q2RUI8
- SMR:   Q2RUI8
- STRING:   Q2RUI8
- GeneID:   3834821
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1406
- NMPDR:   fig|1085.1.peg.2960
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   AENNDTV
- PhylomeDB:   Q2RUI8
- ProtClustDB:   CLSK2773215
- BioCyc:   RRUB269796:RRU_A1406-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 38945; Mature: 38814

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 184-184 ACT_SITE 210-210 ACT_SITE 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRKIRVLIVDDSASVRQTMTEILESDPGIEVIGTAPDPYVAARRIQQEVPDVITLDVEM
CCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEEEC
PRMDGITFLRKIMAQRPIPVVVCSSLTETDSETASQAWEAGAVEIILKPRVGTAQFLLES
CCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHC
KIHICDVVKAAAGARLRSMPAAGRANRPRVPEKKLTADAVLPPPVAGRNAMARTTESVIC
CHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHCCCCEEE
IGASTGGTESLREVLEALPAASPAIVIVQHMPEKFTEAFARRLDSLCDMEVKEAVDGDTV
EECCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHCCCCCE
MRGRVLIAPGNHHMLLQRSGARYYVSVKDGPLVSRHRPSVDVLFRSAASHAGSNAVGIIM
EECEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEE
TGMGDDGARGLLEMRKAGAYTIAQDEATSVVFGMPKEAIALGAADKILPLEMLAMEILRA
ECCCCCHHHHHHHHHHCCCEEEECCCCCEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHC
GNR
CCC
>Mature Secondary Structure 
PRKIRVLIVDDSASVRQTMTEILESDPGIEVIGTAPDPYVAARRIQQEVPDVITLDVEM
CCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEEEC
PRMDGITFLRKIMAQRPIPVVVCSSLTETDSETASQAWEAGAVEIILKPRVGTAQFLLES
CCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHC
KIHICDVVKAAAGARLRSMPAAGRANRPRVPEKKLTADAVLPPPVAGRNAMARTTESVIC
CHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHCCCCEEE
IGASTGGTESLREVLEALPAASPAIVIVQHMPEKFTEAFARRLDSLCDMEVKEAVDGDTV
EECCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHCCCCCE
MRGRVLIAPGNHHMLLQRSGARYYVSVKDGPLVSRHRPSVDVLFRSAASHAGSNAVGIIM
EECEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEE
TGMGDDGARGLLEMRKAGAYTIAQDEATSVVFGMPKEAIALGAADKILPLEMLAMEILRA
ECCCCCHHHHHHHHHHCCCEEEECCCCCEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHC
GNR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA