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The map label for this gene is anfK [H]

Identifier: 83592728

GI number: 83592728

Start: 1643287

End: 1644675

Strand: Reverse

Name: anfK [H]

Synonym: Rru_A1392

Alternate gene names: 83592728

Gene position: 1644675-1643287 (Counterclockwise)

Preceding gene: 83592729

Following gene: 83592727

Centisome position: 37.78

GC content: 60.26

Gene sequence:

>1389_bases
ATGTCCTGCGAATTAAGGAAGAAAGAGCGCATCGGCACGATCAATCCGATCTTCACCTGCCAGCCGGCCGGCGCCCAATA
CGCCAGCATCGGCATCAAGGATTGCATCGGCATCGTTCACGGCGGCCAGGGCTGCGTCATGTTCGTCCGCCTGCTGATCT
CCCAGCACCTCAAGGAAAGCTTCGAAATCGCCTCGTCCTCGGTTCACGAAGACGGCGCGGTGTTCGGCGCCCTTGACCGG
GTGGAACAGGCCGTCGACGTGCTGCTGATGCGCTACCCCGACGTCAAGGTCATCCCGATCATCACCACCTGCTCGACCGA
GGTCATCGGCGATGATGTCGATGGCGTGGTCCGCAAGCTGGAAGAAGGCCTGCTCAAGGAGAAGTTTCCCGACCGCGAGG
TCCATCTGATCCCGATCCACACGCCCAGCTTCGTCGGGTCGATGGTCACCGGATACGACGTGGCGGTGCGCGACTTCGTC
AAGGCCTTCGCCAAAAAGGGCAAACCCAGCCTGAAGATCAATCTGATCACCGGCTGGGTCAATCCGGGCGACGTGAAGGA
ACTCAAGCATCTGCTCGCCGAAATGCGGGTCGAGGCCAATGTCTTGTTCGAGATCGAAAGCTTCGATTCGCCGTTGATGC
CCGATAAAAGCGCCGTCTCGCACGGCAGCACGACGATCGACGATCTGATCGACACCGCCAATGCCCAGGGCACCATCGCC
CTGAACCGCTATGAAGGGGCCAAGGCCGCCGAGTTCTTGCAAAAGGGCTTCGACGTGCCCGCCGTCATCGGGCCGACGCC
GATCGGCATCCGCAACACCGACACCTTCTTGCAGAACCTGAAGATCATGACCGGCAAGGCGATCCCCCCATCGCTGGTCC
GCGAACGCGGCATCGCCATCGACGCCCTGACCGATCTTGTCCATATGTTCCTGGCCGACAAGAAGGTGGCGATCTATGGC
AATCCCGATCTGGTGATCGGTCTGGCCGAGTTCTGCCTCGATCTGGAAATGAAGCCGATGCTGTTGCTGCTCGGCGACGA
GAACGCCGGCTATGAGACCGATCCCCGCATCAAGGCGCTTCAGGAGCGCGTCGACCACGATATGGAGATCATCACCAACG
CCGATCTGTGGGAACTGGAGGACCGGGTTAAGAACAAGGGTCTCGAACTCGACCTGATCCTTGGCCATTCCAAGGGCCGC
TTCACCGCCATCGACAACAACATCCCGATGGTCCGCGTCGGCTTCCCCACCTATGACCGCGCCGGCCTCTATCGCCATCC
GGTCGTCGGCTACGCCGGAGCCACCTGGTTGGCCGAAGCCATGGCCAATACCCTGTTCACCGACATGGAATACAAGAAGA
ACAAGGAGTGGATCCTCAACGTCTGGTAA

Upstream 100 bases:

>100_bases
AGGCCTTGATGGGCGCGCTGCACGAGCGCCTCGACCACCTGACCATCACCGGGTCTCTCAACCTGGAACTGACCGACCAA
CATTATTAGGGGATCGCACC

Downstream 100 bases:

>100_bases
GCCTCCTCCCTTGCGTGTCAGGCCCTCCCCCCGGCCCGGGGATGCCTGACACCCCCGCTCTGGGCCCGCCCCCTGGAAAG
GAGCCAGCATGCAAATCGCC

Product: nitrogenase

Products: NA

Alternate protein names: Dinitrogenase 3 subunit beta; Nitrogenase component I [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSCELRKKERIGTINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLISQHLKESFEIASSSVHEDGAVFGALDR
VEQAVDVLLMRYPDVKVIPIITTCSTEVIGDDVDGVVRKLEEGLLKEKFPDREVHLIPIHTPSFVGSMVTGYDVAVRDFV
KAFAKKGKPSLKINLITGWVNPGDVKELKHLLAEMRVEANVLFEIESFDSPLMPDKSAVSHGSTTIDDLIDTANAQGTIA
LNRYEGAKAAEFLQKGFDVPAVIGPTPIGIRNTDTFLQNLKIMTGKAIPPSLVRERGIAIDALTDLVHMFLADKKVAIYG
NPDLVIGLAEFCLDLEMKPMLLLLGDENAGYETDPRIKALQERVDHDMEIITNADLWELEDRVKNKGLELDLILGHSKGR
FTAIDNNIPMVRVGFPTYDRAGLYRHPVVGYAGATWLAEAMANTLFTDMEYKKNKEWILNVW

Sequences:

>Translated_462_residues
MSCELRKKERIGTINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLISQHLKESFEIASSSVHEDGAVFGALDR
VEQAVDVLLMRYPDVKVIPIITTCSTEVIGDDVDGVVRKLEEGLLKEKFPDREVHLIPIHTPSFVGSMVTGYDVAVRDFV
KAFAKKGKPSLKINLITGWVNPGDVKELKHLLAEMRVEANVLFEIESFDSPLMPDKSAVSHGSTTIDDLIDTANAQGTIA
LNRYEGAKAAEFLQKGFDVPAVIGPTPIGIRNTDTFLQNLKIMTGKAIPPSLVRERGIAIDALTDLVHMFLADKKVAIYG
NPDLVIGLAEFCLDLEMKPMLLLLGDENAGYETDPRIKALQERVDHDMEIITNADLWELEDRVKNKGLELDLILGHSKGR
FTAIDNNIPMVRVGFPTYDRAGLYRHPVVGYAGATWLAEAMANTLFTDMEYKKNKEWILNVW
>Mature_461_residues
SCELRKKERIGTINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLISQHLKESFEIASSSVHEDGAVFGALDRV
EQAVDVLLMRYPDVKVIPIITTCSTEVIGDDVDGVVRKLEEGLLKEKFPDREVHLIPIHTPSFVGSMVTGYDVAVRDFVK
AFAKKGKPSLKINLITGWVNPGDVKELKHLLAEMRVEANVLFEIESFDSPLMPDKSAVSHGSTTIDDLIDTANAQGTIAL
NRYEGAKAAEFLQKGFDVPAVIGPTPIGIRNTDTFLQNLKIMTGKAIPPSLVRERGIAIDALTDLVHMFLADKKVAIYGN
PDLVIGLAEFCLDLEMKPMLLLLGDENAGYETDPRIKALQERVDHDMEIITNADLWELEDRVKNKGLELDLILGHSKGRF
TAIDNNIPMVRVGFPTYDRAGLYRHPVVGYAGATWLAEAMANTLFTDMEYKKNKEWILNVW

Specific function: This iron-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation. Other nitrogenase complexes utilize a molybdenum-iron protein or a vanadium-iron protein [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR014280 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 51075; Mature: 50944

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCELRKKERIGTINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLISQHLKES
CCCCCCCHHCCCCCCCEEEECCCCCCHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FEIASSSVHEDGAVFGALDRVEQAVDVLLMRYPDVKVIPIITTCSTEVIGDDVDGVVRKL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHCCCCHHHHHHHH
EEGLLKEKFPDREVHLIPIHTPSFVGSMVTGYDVAVRDFVKAFAKKGKPSLKINLITGWV
HHHHHHHCCCCCCEEEEEECCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEEECC
NPGDVKELKHLLAEMRVEANVLFEIESFDSPLMPDKSAVSHGSTTIDDLIDTANAQGTIA
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHCCCCCCHHHHHHCCCCCEEEE
LNRYEGAKAAEFLQKGFDVPAVIGPTPIGIRNTDTFLQNLKIMTGKAIPPSLVRERGIAI
EECCCCCHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHEECCCCCHHHHHHCCCCH
DALTDLVHMFLADKKVAIYGNPDLVIGLAEFCLDLEMKPMLLLLGDENAGYETDPRIKAL
HHHHHHHHHHHCCCEEEEECCCCHHHEHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHH
QERVDHDMEIITNADLWELEDRVKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDR
HHHHCCCCEEEECCCHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCCEEEECCCCCCC
AGLYRHPVVGYAGATWLAEAMANTLFTDMEYKKNKEWILNVW
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCEEEEEC
>Mature Secondary Structure 
SCELRKKERIGTINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLISQHLKES
CCCCCCHHCCCCCCCEEEECCCCCCHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FEIASSSVHEDGAVFGALDRVEQAVDVLLMRYPDVKVIPIITTCSTEVIGDDVDGVVRKL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHCCCCHHHHHHHH
EEGLLKEKFPDREVHLIPIHTPSFVGSMVTGYDVAVRDFVKAFAKKGKPSLKINLITGWV
HHHHHHHCCCCCCEEEEEECCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEEECC
NPGDVKELKHLLAEMRVEANVLFEIESFDSPLMPDKSAVSHGSTTIDDLIDTANAQGTIA
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHCCCCCCHHHHHHCCCCCEEEE
LNRYEGAKAAEFLQKGFDVPAVIGPTPIGIRNTDTFLQNLKIMTGKAIPPSLVRERGIAI
EECCCCCHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHEECCCCCHHHHHHCCCCH
DALTDLVHMFLADKKVAIYGNPDLVIGLAEFCLDLEMKPMLLLLGDENAGYETDPRIKAL
HHHHHHHHHHHCCCEEEEECCCCHHHEHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHH
QERVDHDMEIITNADLWELEDRVKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDR
HHHHCCCCEEEECCCHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCCEEEECCCCCCC
AGLYRHPVVGYAGATWLAEAMANTLFTDMEYKKNKEWILNVW
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644222; 8392330 [H]