Click here to switch to the map view.

The map label for this gene is anfA [H]

Identifier: 83592724

GI number: 83592724

Start: 1639371

End: 1640972

Strand: Direct

Name: anfA [H]

Synonym: Rru_A1388

Alternate gene names: 83592724

Gene position: 1639371-1640972 (Clockwise)

Preceding gene: 83592722

Following gene: 83592725

Centisome position: 37.66

GC content: 61.86

Gene sequence:

>1602_bases
ATGCTTATGGACTATACCTATGCGATTGATCTCGACGATTTCGTCGATGATTCCACCGACTGCAAGACCGGCGAATGTCG
TGTCGCCGTTCTGCCGATCCTGTTCCAGATCAGCCAGATCATTTCCGAGAGCGAGGATTTGCCCCGGTCTCTGGCGATCA
TTCTCAAGGTCATGCAGCAGCGCATGCGCATCGGCCGCGGAACCGTCAGTCTTTATGACCGCGAAAGCGGCAAGATCTTC
GTTCACGAAAGCTTTGGCGGCCGGGACGACCAGCAGGCCCTGGGCGCCAGCGGGCCGGGGCGCGGCATCACCGCCAAGGT
GGTGGACTCGGGCAAGGCGATCATCGTGCCCGAACTGCGCGACAGCCCGACCAAGCCCAACCGCACCCAGCTTCAGGCCG
ATGGCAGCGATTTGGCGGTGTCGTTCTTCTGCGTGCCGATCCTGCGCGGTCGCAAGGTTCTGGGCACCATCAGCGCCGAG
CGCGTCTATGCCAATCGCCGGCTGTTGAAGCAGGACGTGGAACTGATCGCCACCATCGCCTCGATGATCGCCCCGGCGGT
GGAGCTGTATCTTTTGGAGAACATCGACAAGGTGCGGCTGGAAAACGAGAATCGCCGTCTGCACGACGCCCTGAAAAGCC
GCTTCAAACCTACCAATATCATCGGCACCTCCAAGCCGATGCAAGAGGTCTATGATCTGATCCACAAGGTGGCGATGACC
AAGGCCACCGTGCTGATCCTCGGGGAAAGCGGCGTCGGCAAGGAATTGGTCGCCAGCGCCATCCATTACAACGGCGCCAC
CGCCGAAGGGCCGTTCATCAAGTTCAATTGCGCCGCCTTGCCCGAAAGCCTAGGCGAGTCCGAGTTGTTCGGTCACGAGA
AAGGCGCCTTCACCGGCGCCATCGCCCAGCGCAAGGGGCGCTTCGAGATGGCCGACGGCGGCACCATCTTTCTCGACGAG
GTTGGCGAGCTGAGTTTGGCCATGCAGGCCAAGCTGTTGCGGGTGCTCCAGGAAAAGACCTTCGAGCGCGTCGGCGGCGG
CCGGTCGGTGCGCGTCGACGTGCGGATCATCGCCGCCACCAACCGCAATCTGCCCGAGATGGTCGAGAAGGGCACCTTCC
GCGAGGATCTGTTCTACCGGCTCAATGTCTTCCCCATCACCTTGCCGCCGCTGCGCGACCGGGGCAGCGACGTGATCTTG
CTGGTCGATCATTTCATCGCCCGCCACGCCGCCGAGGGTGGGCGCGAGGCCAAACGGGTGTCGACCCCGGCCTTGACCAT
GCTGATGGCCTATCACTGGCCGGGCAATGTGCGCGAACTGGAAAACGTTATCGAACGCTCGGTGATCTTGTCGGAAGACG
GGGTGATCCACGGCTATAATCTGCCGCCCTCGCTGCAGACGGCAACGGAGACCGGCACGTCGTTTGGCTGCGGCCTGGAA
GCCAAGCTGCAGGCGGTGGAATACGAAATGATCGTCGAGGCCCTGAAAACCCATGGCGGCAACGCCACCGAGGCGGCCAA
GGAGTTGGGGCTGACCCGGCGCATCCTTGGCCTGCGCATGGAAAAATACGCCCTCAATTACAAGACTTATCGCAAGCGCT
GA

Upstream 100 bases:

>100_bases
GGAAGGCGCCGGTGCCGCCATGGGTGCGTAATGCCAAGCCGGCTTTTGGGCCGGGCGAGGGTGGGACGCGATCACGGCGG
CGGATCGGGAGTGATCCGAG

Downstream 100 bases:

>100_bases
GGGGCGCGGCGGGGGCGTTTCGTTCGGCGGGATCGCTTGGGTTGTGCTATGCTGCGCTGGCCCCGCCGTCCTTTCGCCGA
CGAGATATCCCGATCATGGA

Product: NifA subfamily transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MLMDYTYAIDLDDFVDDSTDCKTGECRVAVLPILFQISQIISESEDLPRSLAIILKVMQQRMRIGRGTVSLYDRESGKIF
VHESFGGRDDQQALGASGPGRGITAKVVDSGKAIIVPELRDSPTKPNRTQLQADGSDLAVSFFCVPILRGRKVLGTISAE
RVYANRRLLKQDVELIATIASMIAPAVELYLLENIDKVRLENENRRLHDALKSRFKPTNIIGTSKPMQEVYDLIHKVAMT
KATVLILGESGVGKELVASAIHYNGATAEGPFIKFNCAALPESLGESELFGHEKGAFTGAIAQRKGRFEMADGGTIFLDE
VGELSLAMQAKLLRVLQEKTFERVGGGRSVRVDVRIIAATNRNLPEMVEKGTFREDLFYRLNVFPITLPPLRDRGSDVIL
LVDHFIARHAAEGGREAKRVSTPALTMLMAYHWPGNVRELENVIERSVILSEDGVIHGYNLPPSLQTATETGTSFGCGLE
AKLQAVEYEMIVEALKTHGGNATEAAKELGLTRRILGLRMEKYALNYKTYRKR

Sequences:

>Translated_533_residues
MLMDYTYAIDLDDFVDDSTDCKTGECRVAVLPILFQISQIISESEDLPRSLAIILKVMQQRMRIGRGTVSLYDRESGKIF
VHESFGGRDDQQALGASGPGRGITAKVVDSGKAIIVPELRDSPTKPNRTQLQADGSDLAVSFFCVPILRGRKVLGTISAE
RVYANRRLLKQDVELIATIASMIAPAVELYLLENIDKVRLENENRRLHDALKSRFKPTNIIGTSKPMQEVYDLIHKVAMT
KATVLILGESGVGKELVASAIHYNGATAEGPFIKFNCAALPESLGESELFGHEKGAFTGAIAQRKGRFEMADGGTIFLDE
VGELSLAMQAKLLRVLQEKTFERVGGGRSVRVDVRIIAATNRNLPEMVEKGTFREDLFYRLNVFPITLPPLRDRGSDVIL
LVDHFIARHAAEGGREAKRVSTPALTMLMAYHWPGNVRELENVIERSVILSEDGVIHGYNLPPSLQTATETGTSFGCGLE
AKLQAVEYEMIVEALKTHGGNATEAAKELGLTRRILGLRMEKYALNYKTYRKR
>Mature_533_residues
MLMDYTYAIDLDDFVDDSTDCKTGECRVAVLPILFQISQIISESEDLPRSLAIILKVMQQRMRIGRGTVSLYDRESGKIF
VHESFGGRDDQQALGASGPGRGITAKVVDSGKAIIVPELRDSPTKPNRTQLQADGSDLAVSFFCVPILRGRKVLGTISAE
RVYANRRLLKQDVELIATIASMIAPAVELYLLENIDKVRLENENRRLHDALKSRFKPTNIIGTSKPMQEVYDLIHKVAMT
KATVLILGESGVGKELVASAIHYNGATAEGPFIKFNCAALPESLGESELFGHEKGAFTGAIAQRKGRFEMADGGTIFLDE
VGELSLAMQAKLLRVLQEKTFERVGGGRSVRVDVRIIAATNRNLPEMVEKGTFREDLFYRLNVFPITLPPLRDRGSDVIL
LVDHFIARHAAEGGREAKRVSTPALTMLMAYHWPGNVRELENVIERSVILSEDGVIHGYNLPPSLQTATETGTSFGCGLE
AKLQAVEYEMIVEALKTHGGNATEAAKELGLTRRILGLRMEKYALNYKTYRKR

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=333, Percent_Identity=48.948948948949, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI87082117, Length=379, Percent_Identity=44.3271767810026, Blast_Score=295, Evalue=6e-81,
Organism=Escherichia coli, GI1790437, Length=306, Percent_Identity=48.6928104575163, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI1789087, Length=401, Percent_Identity=43.640897755611, Blast_Score=279, Evalue=4e-76,
Organism=Escherichia coli, GI87082152, Length=254, Percent_Identity=54.7244094488189, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI1790299, Length=332, Percent_Identity=44.2771084337349, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI1789233, Length=319, Percent_Identity=42.0062695924765, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1788905, Length=314, Percent_Identity=41.7197452229299, Blast_Score=235, Evalue=6e-63,
Organism=Escherichia coli, GI87081872, Length=310, Percent_Identity=43.2258064516129, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1787583, Length=318, Percent_Identity=39.3081761006289, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1786524, Length=350, Percent_Identity=38.8571428571429, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI1789828, Length=269, Percent_Identity=37.9182156133829, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI87081858, Length=311, Percent_Identity=33.1189710610932, Blast_Score=155, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 58909; Mature: 58909

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMDYTYAIDLDDFVDDSTDCKTGECRVAVLPILFQISQIISESEDLPRSLAIILKVMQQ
CCEEEEEEEECHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RMRIGRGTVSLYDRESGKIFVHESFGGRDDQQALGASGPGRGITAKVVDSGKAIIVPELR
HHHHCCCEEEEEECCCCEEEEECCCCCCCHHHHCCCCCCCCCEEEEEECCCCEEEEECCC
DSPTKPNRTQLQADGSDLAVSFFCVPILRGRKVLGTISAERVYANRRLLKQDVELIATIA
CCCCCCCCCEEEECCCCCEEHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHH
SMIAPAVELYLLENIDKVRLENENRRLHDALKSRFKPTNIIGTSKPMQEVYDLIHKVAMT
HHHHHHHHHHHHHCCHHEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
KATVLILGESGVGKELVASAIHYNGATAEGPFIKFNCAALPESLGESELFGHEKGAFTGA
HEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEEHHHCCHHCCCHHHCCCCCCCHHHH
IAQRKGRFEMADGGTIFLDEVGELSLAMQAKLLRVLQEKTFERVGGGRSVRVDVRIIAAT
HHHCCCCEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEC
NRNLPEMVEKGTFREDLFYRLNVFPITLPPLRDRGSDVILLVDHFIARHAAEGGREAKRV
CCCCHHHHHCCCCHHHHEEEEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHCCCCHHHHH
STPALTMLMAYHWPGNVRELENVIERSVILSEDGVIHGYNLPPSLQTATETGTSFGCGLE
CCHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCEEECCCCCCCCCHHHHCCCCCCCCCH
AKLQAVEYEMIVEALKTHGGNATEAAKELGLTRRILGLRMEKYALNYKTYRKR
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MLMDYTYAIDLDDFVDDSTDCKTGECRVAVLPILFQISQIISESEDLPRSLAIILKVMQQ
CCEEEEEEEECHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RMRIGRGTVSLYDRESGKIFVHESFGGRDDQQALGASGPGRGITAKVVDSGKAIIVPELR
HHHHCCCEEEEEECCCCEEEEECCCCCCCHHHHCCCCCCCCCEEEEEECCCCEEEEECCC
DSPTKPNRTQLQADGSDLAVSFFCVPILRGRKVLGTISAERVYANRRLLKQDVELIATIA
CCCCCCCCCEEEECCCCCEEHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHH
SMIAPAVELYLLENIDKVRLENENRRLHDALKSRFKPTNIIGTSKPMQEVYDLIHKVAMT
HHHHHHHHHHHHHCCHHEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
KATVLILGESGVGKELVASAIHYNGATAEGPFIKFNCAALPESLGESELFGHEKGAFTGA
HEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEEHHHCCHHCCCHHHCCCCCCCHHHH
IAQRKGRFEMADGGTIFLDEVGELSLAMQAKLLRVLQEKTFERVGGGRSVRVDVRIIAAT
HHHCCCCEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEC
NRNLPEMVEKGTFREDLFYRLNVFPITLPPLRDRGSDVILLVDHFIARHAAEGGREAKRV
CCCCHHHHHCCCCHHHHEEEEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHCCCCHHHHH
STPALTMLMAYHWPGNVRELENVIERSVILSEDGVIHGYNLPPSLQTATETGTSFGCGLE
CCHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCEEECCCCCCCCCHHHHCCCCCCCCCH
AKLQAVEYEMIVEALKTHGGNATEAAKELGLTRRILGLRMEKYALNYKTYRKR
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]