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The map label for this gene is tpiA [H]

Identifier: 83592694

GI number: 83592694

Start: 1599769

End: 1600545

Strand: Reverse

Name: tpiA [H]

Synonym: Rru_A1358

Alternate gene names: 83592694

Gene position: 1600545-1599769 (Counterclockwise)

Preceding gene: 83592695

Following gene: 83592693

Centisome position: 36.77

GC content: 69.11

Gene sequence:

>777_bases
ATGAACGCCCGATTGTGGATCGGAACAAGCTGGAAGATGCACAAGACCATCGCCGAGGCCGAGGCCTATGTCGGCGAACT
GACCGCCTCGCCGCTGCTCGACCGGGTCGAGGCGTTCATCGTGCCGCCCTTCACCGCCCTGGCCGCCGTCCACCGGGCTT
TGGGCGACGCCCCGGTCCACCTGGGCGCCCAGACCATGCATTGGCAAGACGAGGGGGCGCAGACCGGCGAGATTTCCCCG
CTGATGCTGCGCGATTGCGGCGTCGGTATCGTCGAACTCGGCCATTCCGAGCGCCGCGCCAGCCATGGGGAAACCGACGC
CACCGTCAACGCCAAGGTGGCCAGCGCCCTGCGCCACGGTTTGCGGCCGCTGATCTGCGTTGGCGACCAGGGCGACGACC
TGGACGCCGGAGCCTCGATCGAAACGGTCCTGCGCCAAGTCAAGCTGGCCATCAAAGGCATCGACACGGCCAGCCTGGGC
CGGGCGCTCATCGCCTATGAACCGGTCTGGGCGATCGGCGAAGCCGGGCGACCGGCCGAACCGGCGCATGTGGCCGAGGT
TCATGGCGCCCTGCGCGCCATGCTCGGCCAACGGGGCTTGGCTGCCCTGCCCCTGCTTTATGGCGGCAGCGTCGATCGCG
CCAATGCCGGGGCTCTCACCGCCCTAGCCAACGTTGACGGCCTGTTCGTCGGCCGCGCCGCCTGGACGGCGCGCGGCTTC
GAAGAGGTGATCGAGGCCGCTTTGCACGGGGCCAAGGACCGGGCGGGTCTTGCGTAA

Upstream 100 bases:

>100_bases
GCATTACAAAAAGCTGGCCAGCTGACCCCCCTATCGCCGATCCCGTCTTGTTCGAGGCCCGGCCGCCGAGAAGCGGCCGG
ATAGAGAAGGAGTTTGTCCG

Downstream 100 bases:

>100_bases
TTCCTTAAATCGCAATCGATTTAGGGATAAATTTATGCGACACATCAAAGCGTTACAGCATCTCAAAGCCGTGCTGCCAA
CACCTTCGGGACGCTGTAAC

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM 2; Triose-phosphate isomerase 2 [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MNARLWIGTSWKMHKTIAEAEAYVGELTASPLLDRVEAFIVPPFTALAAVHRALGDAPVHLGAQTMHWQDEGAQTGEISP
LMLRDCGVGIVELGHSERRASHGETDATVNAKVASALRHGLRPLICVGDQGDDLDAGASIETVLRQVKLAIKGIDTASLG
RALIAYEPVWAIGEAGRPAEPAHVAEVHGALRAMLGQRGLAALPLLYGGSVDRANAGALTALANVDGLFVGRAAWTARGF
EEVIEAALHGAKDRAGLA

Sequences:

>Translated_258_residues
MNARLWIGTSWKMHKTIAEAEAYVGELTASPLLDRVEAFIVPPFTALAAVHRALGDAPVHLGAQTMHWQDEGAQTGEISP
LMLRDCGVGIVELGHSERRASHGETDATVNAKVASALRHGLRPLICVGDQGDDLDAGASIETVLRQVKLAIKGIDTASLG
RALIAYEPVWAIGEAGRPAEPAHVAEVHGALRAMLGQRGLAALPLLYGGSVDRANAGALTALANVDGLFVGRAAWTARGF
EEVIEAALHGAKDRAGLA
>Mature_258_residues
MNARLWIGTSWKMHKTIAEAEAYVGELTASPLLDRVEAFIVPPFTALAAVHRALGDAPVHLGAQTMHWQDEGAQTGEISP
LMLRDCGVGIVELGHSERRASHGETDATVNAKVASALRHGLRPLICVGDQGDDLDAGASIETVLRQVKLAIKGIDTASLG
RALIAYEPVWAIGEAGRPAEPAHVAEVHGALRAMLGQRGLAALPLLYGGSVDRANAGALTALANVDGLFVGRAAWTARGF
EEVIEAALHGAKDRAGLA

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4507645, Length=247, Percent_Identity=36.4372469635627, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI226529917, Length=247, Percent_Identity=36.4372469635627, Blast_Score=138, Evalue=4e-33,
Organism=Escherichia coli, GI1790353, Length=211, Percent_Identity=36.9668246445498, Blast_Score=127, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17536593, Length=239, Percent_Identity=36.8200836820084, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320255, Length=238, Percent_Identity=31.9327731092437, Blast_Score=123, Evalue=4e-29,
Organism=Drosophila melanogaster, GI28572008, Length=255, Percent_Identity=37.6470588235294, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28572006, Length=255, Percent_Identity=37.6470588235294, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28572004, Length=256, Percent_Identity=37.890625, Blast_Score=129, Evalue=2e-30,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 27017; Mature: 27017

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNARLWIGTSWKMHKTIAEAEAYVGELTASPLLDRVEAFIVPPFTALAAVHRALGDAPVH
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEE
LGAQTMHWQDEGAQTGEISPLMLRDCGVGIVELGHSERRASHGETDATVNAKVASALRHG
CCCEEEEECCCCCCCCCCCCEEHHHCCCCCEECCCCHHHCCCCCCCCCHHHHHHHHHHHC
LRPLICVGDQGDDLDAGASIETVLRQVKLAIKGIDTASLGRALIAYEPVWAIGEAGRPAE
CCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCHHEECCHHHCCCCCCCCC
PAHVAEVHGALRAMLGQRGLAALPLLYGGSVDRANAGALTALANVDGLFVGRAAWTARGF
CHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCEEECHHHHHHHHH
EEVIEAALHGAKDRAGLA
HHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNARLWIGTSWKMHKTIAEAEAYVGELTASPLLDRVEAFIVPPFTALAAVHRALGDAPVH
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEE
LGAQTMHWQDEGAQTGEISPLMLRDCGVGIVELGHSERRASHGETDATVNAKVASALRHG
CCCEEEEECCCCCCCCCCCCEEHHHCCCCCEECCCCHHHCCCCCCCCCHHHHHHHHHHHC
LRPLICVGDQGDDLDAGASIETVLRQVKLAIKGIDTASLGRALIAYEPVWAIGEAGRPAE
CCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCHHEECCHHHCCCCCCCCC
PAHVAEVHGALRAMLGQRGLAALPLLYGGSVDRANAGALTALANVDGLFVGRAAWTARGF
CHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCEEECHHHHHHHHH
EEVIEAALHGAKDRAGLA
HHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA