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The map label for this gene is mcl2 [H]

Identifier: 83592536

GI number: 83592536

Start: 1417819

End: 1418730

Strand: Direct

Name: mcl2 [H]

Synonym: Rru_A1200

Alternate gene names: 83592536

Gene position: 1417819-1418730 (Clockwise)

Preceding gene: 83592532

Following gene: 83592537

Centisome position: 32.57

GC content: 68.75

Gene sequence:

>912_bases
ATGTCGTTTTCCGTGCGCCCGCGCCGCAGCGTGCTTTATATGCCCGGGTCGAATACCCGGGCCCTTGAAAAGGCCAGGAC
CCTGGCCGCCGACGGGCTGATCCTTGATCTCGAGGACGCCACCGCCCCCGATGCCAAGGAAGACGCCCGGGGCCACGTCT
GCGCCGCCGCCCAGGCCCGCAGCTATGGCCAGCGCGAGGTGCTGATCCGGGTCAATGGCCTGAATACCGCCTGGGGCCAT
GCCGATCTGGTGGCCTGCGCCACCTCGGGCTGCGACGGGGTGCTGCTGCCCAAGGTGGAAAGCGCCGACGGCGTGCGCCA
GGCCGAGGCCATTTTGCGGGCGGCCGGCGCCCCGGACGATCTGGCCCTGTGGTGCATGATGGAAACCCCGCGCGGCGTGC
TTGATGTCGCGGCCATCGCCGGCGCCTCGCCGCGGCTGGCCGGGCTGGTGATGGGAACCTCCGATCTCGCCAAGGATCTG
CATTGCGCCCATACCCTCGAGCGCCTGCCGCTAATCACCGGTCTTGGGCTCTGCCTGCTGGCGGCGCGGGCCAATGGGCT
GGCGATTTTGGACGGGGTGCATCTTGATCTTCAAGACGACGAGGGGTTCGCCCTGTCCTGCGCCCAGGGTCGCGAGCTTG
GCTTTGACGGCAAGACCCTGATCCACCCCAAGACCATCGGCGCCGCCAATGCCGCCTTCGCGCCTTCGGCCGAGGAGGTG
GCGTGGTCGCGCCGGATCATCGAGGCCCATGCCGAGGCGGTGCGCGAGGGCAAGGGCGTGGTTCTGGTCGATGGCAAGTT
GGTGGAGAACCTTCACGTGGAAAGTGCCCGCCGGCTTGTCACGCTCGACGAGAAGATCGCCGCCCTTGGCGGGGATCTGG
CGCAGGACCGGGCGATGGGGAGCCAAGCGTGA

Upstream 100 bases:

>100_bases
GAGGCGCGTTGTTTAAGTGCGTCCCCAAGCGGGCGAAGACCGGGAAGAGTCGCCCCTACCGTCCAATGAGCAGCCCCAAC
AGGCAAGGAGGTTTTACACC

Downstream 100 bases:

>100_bases
TGGGCAAGACCAATCCGGGTAATTTCTTCGAGGACTTCGCTCCCGGCCAGCAGCTTGTCCATGCCACGCCGCGCACGCTG
ACCGAGGGCGACGCCGCCCT

Product: citrate lyase

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSFSVRPRRSVLYMPGSNTRALEKARTLAADGLILDLEDATAPDAKEDARGHVCAAAQARSYGQREVLIRVNGLNTAWGH
ADLVACATSGCDGVLLPKVESADGVRQAEAILRAAGAPDDLALWCMMETPRGVLDVAAIAGASPRLAGLVMGTSDLAKDL
HCAHTLERLPLITGLGLCLLAARANGLAILDGVHLDLQDDEGFALSCAQGRELGFDGKTLIHPKTIGAANAAFAPSAEEV
AWSRRIIEAHAEAVREGKGVVLVDGKLVENLHVESARRLVTLDEKIAALGGDLAQDRAMGSQA

Sequences:

>Translated_303_residues
MSFSVRPRRSVLYMPGSNTRALEKARTLAADGLILDLEDATAPDAKEDARGHVCAAAQARSYGQREVLIRVNGLNTAWGH
ADLVACATSGCDGVLLPKVESADGVRQAEAILRAAGAPDDLALWCMMETPRGVLDVAAIAGASPRLAGLVMGTSDLAKDL
HCAHTLERLPLITGLGLCLLAARANGLAILDGVHLDLQDDEGFALSCAQGRELGFDGKTLIHPKTIGAANAAFAPSAEEV
AWSRRIIEAHAEAVREGKGVVLVDGKLVENLHVESARRLVTLDEKIAALGGDLAQDRAMGSQA
>Mature_302_residues
SFSVRPRRSVLYMPGSNTRALEKARTLAADGLILDLEDATAPDAKEDARGHVCAAAQARSYGQREVLIRVNGLNTAWGHA
DLVACATSGCDGVLLPKVESADGVRQAEAILRAAGAPDDLALWCMMETPRGVLDVAAIAGASPRLAGLVMGTSDLAKDLH
CAHTLERLPLITGLGLCLLAARANGLAILDGVHLDLQDDEGFALSCAQGRELGFDGKTLIHPKTIGAANAAFAPSAEEVA
WSRRIIEAHAEAVREGKGVVLVDGKLVENLHVESARRLVTLDEKIAALGGDLAQDRAMGSQA

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=290, Percent_Identity=27.5862068965517, Blast_Score=127, Evalue=9e-30,
Organism=Escherichia coli, GI87081764, Length=283, Percent_Identity=36.7491166077738, Blast_Score=152, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17557458, Length=304, Percent_Identity=26.3157894736842, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 31733; Mature: 31601

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSVRPRRSVLYMPGSNTRALEKARTLAADGLILDLEDATAPDAKEDARGHVCAAAQAR
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCHHCCCCEEEHHHHH
SYGQREVLIRVNGLNTAWGHADLVACATSGCDGVLLPKVESADGVRQAEAILRAAGAPDD
CCCCEEEEEEEECCCCCCCCHHEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCC
LALWCMMETPRGVLDVAAIAGASPRLAGLVMGTSDLAKDLHCAHTLERLPLITGLGLCLL
EEEEEEECCCCCHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARANGLAILDGVHLDLQDDEGFALSCAQGRELGFDGKTLIHPKTIGAANAAFAPSAEEV
HHCCCCEEEEEEEEEEEECCCCCEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHH
AWSRRIIEAHAEAVREGKGVVLVDGKLVENLHVESARRLVTLDEKIAALGGDLAQDRAMG
HHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHEEEHHHHHHHHCCCHHHHHCCC
SQA
CCC
>Mature Secondary Structure 
SFSVRPRRSVLYMPGSNTRALEKARTLAADGLILDLEDATAPDAKEDARGHVCAAAQAR
CCCCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCHHCCCCEEEHHHHH
SYGQREVLIRVNGLNTAWGHADLVACATSGCDGVLLPKVESADGVRQAEAILRAAGAPDD
CCCCEEEEEEEECCCCCCCCHHEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCC
LALWCMMETPRGVLDVAAIAGASPRLAGLVMGTSDLAKDLHCAHTLERLPLITGLGLCLL
EEEEEEECCCCCHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARANGLAILDGVHLDLQDDEGFALSCAQGRELGFDGKTLIHPKTIGAANAAFAPSAEEV
HHCCCCEEEEEEEEEEEECCCCCEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHH
AWSRRIIEAHAEAVREGKGVVLVDGKLVENLHVESARRLVTLDEKIAALGGDLAQDRAMG
HHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHEEEHHHHHHHHCCCHHHHHCCC
SQA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA