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The map label for this gene is actII-2 [H]

Identifier: 83592450

GI number: 83592450

Start: 1308659

End: 1310119

Strand: Reverse

Name: actII-2 [H]

Synonym: Rru_A1114

Alternate gene names: 83592450

Gene position: 1310119-1308659 (Counterclockwise)

Preceding gene: 83592455

Following gene: 83592447

Centisome position: 30.1

GC content: 65.43

Gene sequence:

>1461_bases
ATGTCCGACACTTGCGTTTCACGAAGCGAAGCTCCTTCCTCGCCATCCCGCCTGGGCGGGGCTTCCGCCCAGTCTCCGCT
CGCCCTTGCCATCCTTTTGCTTGGTGGCTTCATCACCGTCTTCGACCTGTTCGTCGTCAACATCGCCATCCCCTCGATGC
AGAGGGACCTGGGCGCGACGTTCGCCGAGATCGGCTTCGTCATCGCCGGCTATGAACTGGCCTTTGGGGTATTGCTGATC
GCCGGGGGCCGCCTGGGCGACATCCATGGCCGCCGCCGGCTTTTTGCTTTTGGCCTGCTGGGCTTTGGCCTGACGTCACT
TCTGTGCGGTATCGCGCCCAATGCGGCGAGCCTGATCGTCGCCCGCTTCCTGCAAGGGGCGGCGGGGGCGATCTTGTTTC
CCCAGGTCTATGCGTTGTTGCGCGTGATGTACGATGACGCCGGCCGTCGCCGCGCCTTTGGCCTTCTTGGCATGACGCTG
GGCATGGCCGCCATCGCCGGTCAGGTGCTGGGCGGGCTGATCGTCGAAAGCGACCTGTTTGGCCTTGGCTGGCGCATCAT
TTTTTTGATCAATCTGCCCATCAGCCTGGGGGCCGCGCTCTGCGTCCACACCCTGCCGGACTCGAAGGCGCCGGGCGCCA
GCGTCGTCGACTGGCCGGGCGTCGGCCTTGCCACCGCCGGCCTGTTGCTTCTGTTGGTGCCGCTGCTGGAAGGTCCGAAC
AGCGGCTGGCCCGCCTGGACCTGGGCCAGCTTCGCCGCTTCGGCGGCGCTGCTTGCCGCCTTTCTCGCCTGGGAACGCCG
CGCCGCCCATTCGGCCATCGATCTCGGCCTGTTCGCCCGGCGGGGATTCTCGATCGGCGCCGTCGTTGTCTTGTTGATCT
ACTCGACCGCAACGTCGCTGTTTCTCTGCTTCGCCTTGCTCTTGCAATCGGGTTTGGGCCTAACGCCCTTCGCGGCCGGG
GCATTGTTCGCGCCGGCGAGCGTCGGCTTCGTCGCGGCCTCTCTGCTTGCGCCCAAGCTGGTGTCACGCTGGGGCAATGC
CGCCATCTGGGGCGGCGCCCTGATCTACGCCGCGGGGATCGCTTGGTTGATCGCCATGACGTCGCGCCTGACCGGCAAGG
AGGATGCGCTTTCCTTGCTACCGGCGTTGATCTTGTTCGGCTTTGGACAGGGGTTGTCGATGACGCCGCTGCTGAATCTC
GTCATCGGCTTTGTCGAGGACCGTCACGCCGGCATGGCGGCCGGTATCATCGCGACCATGCAGCAGGTGGGCGGCGCTTT
CGGTGTGTCGATCGTTGGCATCTTCTTCGTCTCCCTTCTGTCTGGCCCGTCAAACGGAGCCGAACCCGACCCGAGCCGCT
ACGGCGCCGCTTTTTCCGGGGCGATGCTCTACAACGTCGCCGCCGCTCTTCTCGCTAGCGCGCTGATCGCCTGGATCGCG
CGCAAGAAAGCGGCACGATGA

Upstream 100 bases:

>100_bases
GGCGGCTGTTCTGGGTGTTGCTCATAGTAATAGTATAACTTCCTCTCTAGTAACAGGATTATGCCCGGCCTATCCCGTGG
TTTCAACCACGGGGGTCCCC

Downstream 100 bases:

>100_bases
CGGAAGGGACAGAGGGTGATCTTCCCGCCGTCACACGGTTTCGAGCGGCAGGAAAGCGGCCTCGGGGGCGCCGCAGCGGC
CGGTGGCGCGGAAGGCGCCC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSDTCVSRSEAPSSPSRLGGASAQSPLALAILLLGGFITVFDLFVVNIAIPSMQRDLGATFAEIGFVIAGYELAFGVLLI
AGGRLGDIHGRRRLFAFGLLGFGLTSLLCGIAPNAASLIVARFLQGAAGAILFPQVYALLRVMYDDAGRRRAFGLLGMTL
GMAAIAGQVLGGLIVESDLFGLGWRIIFLINLPISLGAALCVHTLPDSKAPGASVVDWPGVGLATAGLLLLLVPLLEGPN
SGWPAWTWASFAASAALLAAFLAWERRAAHSAIDLGLFARRGFSIGAVVVLLIYSTATSLFLCFALLLQSGLGLTPFAAG
ALFAPASVGFVAASLLAPKLVSRWGNAAIWGGALIYAAGIAWLIAMTSRLTGKEDALSLLPALILFGFGQGLSMTPLLNL
VIGFVEDRHAGMAAGIIATMQQVGGAFGVSIVGIFFVSLLSGPSNGAEPDPSRYGAAFSGAMLYNVAAALLASALIAWIA
RKKAAR

Sequences:

>Translated_486_residues
MSDTCVSRSEAPSSPSRLGGASAQSPLALAILLLGGFITVFDLFVVNIAIPSMQRDLGATFAEIGFVIAGYELAFGVLLI
AGGRLGDIHGRRRLFAFGLLGFGLTSLLCGIAPNAASLIVARFLQGAAGAILFPQVYALLRVMYDDAGRRRAFGLLGMTL
GMAAIAGQVLGGLIVESDLFGLGWRIIFLINLPISLGAALCVHTLPDSKAPGASVVDWPGVGLATAGLLLLLVPLLEGPN
SGWPAWTWASFAASAALLAAFLAWERRAAHSAIDLGLFARRGFSIGAVVVLLIYSTATSLFLCFALLLQSGLGLTPFAAG
ALFAPASVGFVAASLLAPKLVSRWGNAAIWGGALIYAAGIAWLIAMTSRLTGKEDALSLLPALILFGFGQGLSMTPLLNL
VIGFVEDRHAGMAAGIIATMQQVGGAFGVSIVGIFFVSLLSGPSNGAEPDPSRYGAAFSGAMLYNVAAALLASALIAWIA
RKKAAR
>Mature_485_residues
SDTCVSRSEAPSSPSRLGGASAQSPLALAILLLGGFITVFDLFVVNIAIPSMQRDLGATFAEIGFVIAGYELAFGVLLIA
GGRLGDIHGRRRLFAFGLLGFGLTSLLCGIAPNAASLIVARFLQGAAGAILFPQVYALLRVMYDDAGRRRAFGLLGMTLG
MAAIAGQVLGGLIVESDLFGLGWRIIFLINLPISLGAALCVHTLPDSKAPGASVVDWPGVGLATAGLLLLLVPLLEGPNS
GWPAWTWASFAASAALLAAFLAWERRAAHSAIDLGLFARRGFSIGAVVVLLIYSTATSLFLCFALLLQSGLGLTPFAAGA
LFAPASVGFVAASLLAPKLVSRWGNAAIWGGALIYAAGIAWLIAMTSRLTGKEDALSLLPALILFGFGQGLSMTPLLNLV
IGFVEDRHAGMAAGIIATMQQVGGAFGVSIVGIFFVSLLSGPSNGAEPDPSRYGAAFSGAMLYNVAAALLASALIAWIAR
KKAAR

Specific function: Promotes the efflux of actinorhodin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=327, Percent_Identity=30.8868501529052, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789042, Length=303, Percent_Identity=30.03300330033, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1790195, Length=399, Percent_Identity=26.8170426065163, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788392, Length=413, Percent_Identity=26.1501210653753, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081983, Length=417, Percent_Identity=25.8992805755396, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50047; Mature: 49915

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTCVSRSEAPSSPSRLGGASAQSPLALAILLLGGFITVFDLFVVNIAIPSMQRDLGAT
CCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAEIGFVIAGYELAFGVLLIAGGRLGDIHGRRRLFAFGLLGFGLTSLLCGIAPNAASLIV
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ARFLQGAAGAILFPQVYALLRVMYDDAGRRRAFGLLGMTLGMAAIAGQVLGGLIVESDLF
HHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGWRIIFLINLPISLGAALCVHTLPDSKAPGASVVDWPGVGLATAGLLLLLVPLLEGPN
HHHHHHHHEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
SGWPAWTWASFAASAALLAAFLAWERRAAHSAIDLGLFARRGFSIGAVVVLLIYSTATSL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FLCFALLLQSGLGLTPFAAGALFAPASVGFVAASLLAPKLVSRWGNAAIWGGALIYAAGI
HHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
AWLIAMTSRLTGKEDALSLLPALILFGFGQGLSMTPLLNLVIGFVEDRHAGMAAGIIATM
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
QQVGGAFGVSIVGIFFVSLLSGPSNGAEPDPSRYGAAFSGAMLYNVAAALLASALIAWIA
HHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
RKKAAR
HHHCCC
>Mature Secondary Structure 
SDTCVSRSEAPSSPSRLGGASAQSPLALAILLLGGFITVFDLFVVNIAIPSMQRDLGAT
CCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAEIGFVIAGYELAFGVLLIAGGRLGDIHGRRRLFAFGLLGFGLTSLLCGIAPNAASLIV
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ARFLQGAAGAILFPQVYALLRVMYDDAGRRRAFGLLGMTLGMAAIAGQVLGGLIVESDLF
HHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGWRIIFLINLPISLGAALCVHTLPDSKAPGASVVDWPGVGLATAGLLLLLVPLLEGPN
HHHHHHHHEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
SGWPAWTWASFAASAALLAAFLAWERRAAHSAIDLGLFARRGFSIGAVVVLLIYSTATSL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FLCFALLLQSGLGLTPFAAGALFAPASVGFVAASLLAPKLVSRWGNAAIWGGALIYAAGI
HHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
AWLIAMTSRLTGKEDALSLLPALILFGFGQGLSMTPLLNLVIGFVEDRHAGMAAGIIATM
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
QQVGGAFGVSIVGIFFVSLLSGPSNGAEPDPSRYGAAFSGAMLYNVAAALLASALIAWIA
HHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
RKKAAR
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]