Click here to switch to the map view.

The map label for this gene is ruvB

Identifier: 83592425

GI number: 83592425

Start: 1278609

End: 1279661

Strand: Direct

Name: ruvB

Synonym: Rru_A1089

Alternate gene names: 83592425

Gene position: 1278609-1279661 (Clockwise)

Preceding gene: 83592424

Following gene: 83592426

Centisome position: 29.37

GC content: 68.38

Gene sequence:

>1053_bases
ATGGCCGGGCACGAGGAGGAAGATGAGCGCCTGATCTCGGGGCGCCGCCGCGAGGGCGAGGTCGATGCGGCCCTGCGCCC
GCAGTCGCTGGCCGATTTCGTCGGTCAGCGCCAGACCCGTGAAAACCTGGGCGTTTTCGTTCAGGCCGCCCGGGCGCGCG
GCGAGGCGATGGATCACGTGCTGTTCCATGGTCCGCCGGGCCTGGGGAAAACCACGCTCGCCCAGATCGTGGCGCGCGAG
CTTGGCGTGGGGTTTCGCGGCACCAGTGGGCCGATGATCGTCAAGGCCGGCGACCTCGCGGCGATCTTGACCAATCTCGA
GCCGCGCGATGTGCTGTTCATCGACGAAATCCACCGCCTTAATCCCGCCATAGAGGAAGTTTTGTATCCGGCGATGGAGG
ACTTCCAGTTGGACCTCATCATCGGTGAGGGGCCGGCGGCGCGGAGCGTGCGGATCGACCTGCCGCCCTTCACCCTGGTT
GGGGCGACGACCCGGTCGGGCCTCCTGACAACGCCGCTGCGCGACCGCTTTGGCATTCCGCTGCGACTCGACTTCTATGA
AACCGATGAATTGGTCCAGATCGTGACCCGGGGTTCCCGGCTTCTTGGCATGGCTTTGACCGACGAGGGCGCCCGCGAAG
TGGCGCGACGATCGCGGGGCACGCCGCGGGTGGCCGGTCGGCTGCTGCGCCGGGTGCGCGATTTCGCCGCCGTCGCCGGG
CGTTCGCCCGTTGACGCCTTCATCGCCGATGCCGCGCTCAACCGCCTGGAGGTGGACGGGCGCGGGCTTGATGCCATGGA
CCGGCGCTATCTGTCGCGCATGGCCGACCATTATGGCGGCGGGCCGGTCGGGGTCGATACCCTGGCCGCCGCCCTGGCCG
AGGAACGCGATACGGTGGAAGACGTGATCGAGCCCTATTTGATCCAGCAGGGCTTCATCAAGCGGACGCCGCGCGGCCGG
ATGCTGACGGCGATCGCCTGGACCCATCTTGGGCTGACACCGCCGGCCGAGACGCCGCCGGTTCAGCCCGACCTGTGGTC
CGACGCCCCATGA

Upstream 100 bases:

>100_bases
GGTGGGGGCGGCCAGCCGGGCCGCCGGCTTGGGGGCGACCACCGTGCAAGGGGTGATCGGTCTGGCCCTGCGCGATCTCG
GCCGGGGAGGGGCATAATCC

Downstream 100 bases:

>100_bases
CCCAGTCCTTGACGCCGACCAGCGGCCTCTGGCGCCAGGGGTGCCACTTGTTGCCCGTGCGCGTGCATTGGGAAAACACC
GATGCCGGGGGTATCGTCTA

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MAGHEEEDERLISGRRREGEVDAALRPQSLADFVGQRQTRENLGVFVQAARARGEAMDHVLFHGPPGLGKTTLAQIVARE
LGVGFRGTSGPMIVKAGDLAAILTNLEPRDVLFIDEIHRLNPAIEEVLYPAMEDFQLDLIIGEGPAARSVRIDLPPFTLV
GATTRSGLLTTPLRDRFGIPLRLDFYETDELVQIVTRGSRLLGMALTDEGAREVARRSRGTPRVAGRLLRRVRDFAAVAG
RSPVDAFIADAALNRLEVDGRGLDAMDRRYLSRMADHYGGGPVGVDTLAAALAEERDTVEDVIEPYLIQQGFIKRTPRGR
MLTAIAWTHLGLTPPAETPPVQPDLWSDAP

Sequences:

>Translated_350_residues
MAGHEEEDERLISGRRREGEVDAALRPQSLADFVGQRQTRENLGVFVQAARARGEAMDHVLFHGPPGLGKTTLAQIVARE
LGVGFRGTSGPMIVKAGDLAAILTNLEPRDVLFIDEIHRLNPAIEEVLYPAMEDFQLDLIIGEGPAARSVRIDLPPFTLV
GATTRSGLLTTPLRDRFGIPLRLDFYETDELVQIVTRGSRLLGMALTDEGAREVARRSRGTPRVAGRLLRRVRDFAAVAG
RSPVDAFIADAALNRLEVDGRGLDAMDRRYLSRMADHYGGGPVGVDTLAAALAEERDTVEDVIEPYLIQQGFIKRTPRGR
MLTAIAWTHLGLTPPAETPPVQPDLWSDAP
>Mature_349_residues
AGHEEEDERLISGRRREGEVDAALRPQSLADFVGQRQTRENLGVFVQAARARGEAMDHVLFHGPPGLGKTTLAQIVAREL
GVGFRGTSGPMIVKAGDLAAILTNLEPRDVLFIDEIHRLNPAIEEVLYPAMEDFQLDLIIGEGPAARSVRIDLPPFTLVG
ATTRSGLLTTPLRDRFGIPLRLDFYETDELVQIVTRGSRLLGMALTDEGAREVARRSRGTPRVAGRLLRRVRDFAAVAGR
SPVDAFIADAALNRLEVDGRGLDAMDRRYLSRMADHYGGGPVGVDTLAAALAEERDTVEDVIEPYLIQQGFIKRTPRGRM
LTAIAWTHLGLTPPAETPPVQPDLWSDAP

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=331, Percent_Identity=63.1419939577039, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI1787119, Length=230, Percent_Identity=30, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_RHORT (Q2RVF5)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426177.1
- ProteinModelPortal:   Q2RVF5
- SMR:   Q2RVF5
- STRING:   Q2RVF5
- GeneID:   3833396
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1089
- NMPDR:   fig|1085.1.peg.3282
- eggNOG:   COG2255
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- PhylomeDB:   Q2RVF5
- ProtClustDB:   PRK00080
- BioCyc:   RRUB269796:RRU_A1089-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 38303; Mature: 38172

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGHEEEDERLISGRRREGEVDAALRPQSLADFVGQRQTRENLGVFVQAARARGEAMDHV
CCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFHGPPGLGKTTLAQIVARELGVGFRGTSGPMIVKAGDLAAILTNLEPRDVLFIDEIHRL
EEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHCCCCCCEEEHHHHHHC
NPAIEEVLYPAMEDFQLDLIIGEGPAARSVRIDLPPFTLVGATTRSGLLTTPLRDRFGIP
CHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEECCCEEEEECCCCCCCEECCCHHHCCCC
LRLDFYETDELVQIVTRGSRLLGMALTDEGAREVARRSRGTPRVAGRLLRRVRDFAAVAG
EEEECCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
RSPVDAFIADAALNRLEVDGRGLDAMDRRYLSRMADHYGGGPVGVDTLAAALAEERDTVE
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
DVIEPYLIQQGFIKRTPRGRMLTAIAWTHLGLTPPAETPPVQPDLWSDAP
HHHHHHHHHHHHHHCCCCCCEEHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGHEEEDERLISGRRREGEVDAALRPQSLADFVGQRQTRENLGVFVQAARARGEAMDHV
CCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFHGPPGLGKTTLAQIVARELGVGFRGTSGPMIVKAGDLAAILTNLEPRDVLFIDEIHRL
EEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHCCCCCCEEEHHHHHHC
NPAIEEVLYPAMEDFQLDLIIGEGPAARSVRIDLPPFTLVGATTRSGLLTTPLRDRFGIP
CHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEECCCEEEEECCCCCCCEECCCHHHCCCC
LRLDFYETDELVQIVTRGSRLLGMALTDEGAREVARRSRGTPRVAGRLLRRVRDFAAVAG
EEEECCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
RSPVDAFIADAALNRLEVDGRGLDAMDRRYLSRMADHYGGGPVGVDTLAAALAEERDTVE
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
DVIEPYLIQQGFIKRTPRGRMLTAIAWTHLGLTPPAETPPVQPDLWSDAP
HHHHHHHHHHHHHHCCCCCCEEHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA