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The map label for this gene is thiE [H]

Identifier: 83592416

GI number: 83592416

Start: 1272670

End: 1273320

Strand: Reverse

Name: thiE [H]

Synonym: Rru_A1080

Alternate gene names: 83592416

Gene position: 1273320-1272670 (Counterclockwise)

Preceding gene: 83592417

Following gene: 83592415

Centisome position: 29.25

GC content: 69.12

Gene sequence:

>651_bases
GTGTCCGATCCCGCTTGCCGCCTTTATCTGATCACCCCGCCACGCCTGGACGACTGGGCCGCCTTCGCCGAGACCTTGAA
GCGGACCCTGGCCGCCGGGGATGTCGCCTGCCTGCAGCTGCGCATGAAGGACGAAAGCGACGACGCCATCCGCCGGGCCG
TCGCGGCGATCCGTCCGGTCTGCCATGGCGCCGATGTGGCGCTGCTGCTCAACGACCGACCCGACCTCGCCAAGGAAACC
GGCTGCGACGGCGTTCACGTCGGCCAAACCGACGCGTCTTACGCCCAGGCCCGCGCCATCGTCGGCGCCGACGCCATCGT
CGGCGTCACCTGCCACGACAGCCGCCATCTGGCGATGATCGCCGGCGAAGCCGGGGCCGATTACGTGGCCTTCGGCGCCT
TCTTCCCCTCCACCACCAAGGAACCGCCGACCCAGGCCGATCCCGAGATCCTGCGCTGGTGGAGCGAGATGATGATCGTT
CCCTCGGTGGCCATCGGCGGCATCACCGTGGAAAACTGCGCGCCCCTGGTGGAAACCGGCACGGATTTTTTGGCCGTGTG
CAATGGCGTCTGGGGCCACGCCGAGGGCCCGGAGGCCGCCGTCCGCGCCTTCGTCCGCGTCATCGCCGAGGTCTACGCCC
AGCCGGACTAA

Upstream 100 bases:

>100_bases
AAGCCGCCTGCCTGCGGCTGCTGTTGCCAGACGGGCCCGGCTGACGCATGGTGAGGCTCCCTTAACCGCCTTTCGGCCAG
ACCTTTTTGGAATCCGCCCC

Downstream 100 bases:

>100_bases
CAGACCGGTGGTGAATCAAAGCGTCGTCGCGATCGGCTTCGCCTCGCCCTCGGATTCGCCCGCAGCGCAAGTCTTTGCCC
GCTTTGCCATGAATGGCCCG

Product: thiamine-phosphate diphosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MSDPACRLYLITPPRLDDWAAFAETLKRTLAAGDVACLQLRMKDESDDAIRRAVAAIRPVCHGADVALLLNDRPDLAKET
GCDGVHVGQTDASYAQARAIVGADAIVGVTCHDSRHLAMIAGEAGADYVAFGAFFPSTTKEPPTQADPEILRWWSEMMIV
PSVAIGGITVENCAPLVETGTDFLAVCNGVWGHAEGPEAAVRAFVRVIAEVYAQPD

Sequences:

>Translated_216_residues
MSDPACRLYLITPPRLDDWAAFAETLKRTLAAGDVACLQLRMKDESDDAIRRAVAAIRPVCHGADVALLLNDRPDLAKET
GCDGVHVGQTDASYAQARAIVGADAIVGVTCHDSRHLAMIAGEAGADYVAFGAFFPSTTKEPPTQADPEILRWWSEMMIV
PSVAIGGITVENCAPLVETGTDFLAVCNGVWGHAEGPEAAVRAFVRVIAEVYAQPD
>Mature_215_residues
SDPACRLYLITPPRLDDWAAFAETLKRTLAAGDVACLQLRMKDESDDAIRRAVAAIRPVCHGADVALLLNDRPDLAKETG
CDGVHVGQTDASYAQARAIVGADAIVGVTCHDSRHLAMIAGEAGADYVAFGAFFPSTTKEPPTQADPEILRWWSEMMIVP
SVAIGGITVENCAPLVETGTDFLAVCNGVWGHAEGPEAAVRAFVRVIAEVYAQPD

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=167, Percent_Identity=32.9341317365269, Blast_Score=80, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325042, Length=143, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22991; Mature: 22860

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPACRLYLITPPRLDDWAAFAETLKRTLAAGDVACLQLRMKDESDDAIRRAVAAIRPV
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHH
CHGADVALLLNDRPDLAKETGCDGVHVGQTDASYAQARAIVGADAIVGVTCHDSRHLAMI
CCCCCEEEEECCCCCHHHHCCCCCEEECCCCHHHHHHHHHCCCCEEEEEEECCCCEEEEE
AGEAGADYVAFGAFFPSTTKEPPTQADPEILRWWSEMMIVPSVAIGGITVENCAPLVETG
ECCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCEEHHCCCHHHHCC
TDFLAVCNGVWGHAEGPEAAVRAFVRVIAEVYAQPD
CHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDPACRLYLITPPRLDDWAAFAETLKRTLAAGDVACLQLRMKDESDDAIRRAVAAIRPV
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHH
CHGADVALLLNDRPDLAKETGCDGVHVGQTDASYAQARAIVGADAIVGVTCHDSRHLAMI
CCCCCEEEEECCCCCHHHHCCCCCEEECCCCHHHHHHHHHCCCCEEEEEEECCCCEEEEE
AGEAGADYVAFGAFFPSTTKEPPTQADPEILRWWSEMMIVPSVAIGGITVENCAPLVETG
ECCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCEEHHCCCHHHHCC
TDFLAVCNGVWGHAEGPEAAVRAFVRVIAEVYAQPD
CHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA